RAPD optimization for genetic studies with Citrulus lanatus and Sesamum indicum genotypes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2006 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.12702/1984-7033.v06n02a08 http://hdl.handle.net/11449/69276 |
Resumo: | The Random Amplified Polymorphic DNA (RAPD) technique is powerful for DNA polymorphism determinations and is widely used in research involving different organisms, but it is known that RAPD can be affected by many factors that may result in false positive bands and non-reproducible assays. In this study, we analyzed the effect of several factors such as DNA template, primer and Taq DNA polymerase concentrations to optimize and standardize the RAPD technique for further genetic studies with Citrulus lanattus and Sesamum indicum L. The best combination of DNA, Taq DNA polymerase enzyme and primer concentrations in RAPD amplification procedures for sesame and watermelon genotypes was established. |
| id |
UNSP_0149fe9769bcda6c6e9162a1a5b7255b |
|---|---|
| oai_identifier_str |
oai:repositorio.unesp.br:11449/69276 |
| network_acronym_str |
UNSP |
| network_name_str |
Repositório Institucional da UNESP |
| repository_id_str |
2946 |
| spelling |
RAPD optimization for genetic studies with Citrulus lanatus and Sesamum indicum genotypesConditions, watermelon, sesameDNA amplificationCitrullus lanatus var. lanatusSesamum indicumThe Random Amplified Polymorphic DNA (RAPD) technique is powerful for DNA polymorphism determinations and is widely used in research involving different organisms, but it is known that RAPD can be affected by many factors that may result in false positive bands and non-reproducible assays. In this study, we analyzed the effect of several factors such as DNA template, primer and Taq DNA polymerase concentrations to optimize and standardize the RAPD technique for further genetic studies with Citrulus lanattus and Sesamum indicum L. The best combination of DNA, Taq DNA polymerase enzyme and primer concentrations in RAPD amplification procedures for sesame and watermelon genotypes was established.Departamento de Produção Vegetal Faculdade de Ciências Agrárias e Veterinárias (UNESP) Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14.884-900, Jaboticabal, SPDepartamento de Tecnologia UNESPEmbrapa Algodão, Rua Oswaldo Cruz, 1143, Centenário, 58.107-720, Campina Grande, PBAPTA Regional Centro Leste, C. P. 271, 14.001-970, Ribeirão Preto, SPDepartamento de Produção Vegetal Faculdade de Ciências Agrárias e Veterinárias (UNESP) Campus de Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14.884-900, Jaboticabal, SPDepartamento de Tecnologia UNESPUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Agência Paulista de Tecnologia dos Agronegócios (APTA)Di Mauro, Antonio Orlando [UNESP]Di Mauro, Daniela Cristina [UNESP]Capeloto, Andrea [UNESP]Arriel, Nair Helena de CastroCosta, Marcelo Marchi [UNESP]Trevisoli, Sandra Helena UnedaDi Mauro, Sonia Marli Zingaretti [UNESP]2014-05-27T11:22:03Z2014-05-27T11:22:03Z2006-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article167-170application/pdfhttp://dx.doi.org/10.12702/1984-7033.v06n02a08Crop Breeding and Applied Biotechnology, v. 6, n. 2, p. 167-170, 2006.1518-7853http://hdl.handle.net/11449/6927610.12702/1984-7033.v06n02a082-s2.0-411491273632-s2.0-41149127363.pdf1275652518822095Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCrop Breeding and Applied Biotechnology0,609info:eu-repo/semantics/openAccess2024-06-07T13:57:21Zoai:repositorio.unesp.br:11449/69276Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-07T13:57:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
RAPD optimization for genetic studies with Citrulus lanatus and Sesamum indicum genotypes |
| title |
RAPD optimization for genetic studies with Citrulus lanatus and Sesamum indicum genotypes |
| spellingShingle |
RAPD optimization for genetic studies with Citrulus lanatus and Sesamum indicum genotypes Di Mauro, Antonio Orlando [UNESP] Conditions, watermelon, sesame DNA amplification Citrullus lanatus var. lanatus Sesamum indicum |
| title_short |
RAPD optimization for genetic studies with Citrulus lanatus and Sesamum indicum genotypes |
| title_full |
RAPD optimization for genetic studies with Citrulus lanatus and Sesamum indicum genotypes |
| title_fullStr |
RAPD optimization for genetic studies with Citrulus lanatus and Sesamum indicum genotypes |
| title_full_unstemmed |
RAPD optimization for genetic studies with Citrulus lanatus and Sesamum indicum genotypes |
| title_sort |
RAPD optimization for genetic studies with Citrulus lanatus and Sesamum indicum genotypes |
| author |
Di Mauro, Antonio Orlando [UNESP] |
| author_facet |
Di Mauro, Antonio Orlando [UNESP] Di Mauro, Daniela Cristina [UNESP] Capeloto, Andrea [UNESP] Arriel, Nair Helena de Castro Costa, Marcelo Marchi [UNESP] Trevisoli, Sandra Helena Uneda Di Mauro, Sonia Marli Zingaretti [UNESP] |
| author_role |
author |
| author2 |
Di Mauro, Daniela Cristina [UNESP] Capeloto, Andrea [UNESP] Arriel, Nair Helena de Castro Costa, Marcelo Marchi [UNESP] Trevisoli, Sandra Helena Uneda Di Mauro, Sonia Marli Zingaretti [UNESP] |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Agência Paulista de Tecnologia dos Agronegócios (APTA) |
| dc.contributor.author.fl_str_mv |
Di Mauro, Antonio Orlando [UNESP] Di Mauro, Daniela Cristina [UNESP] Capeloto, Andrea [UNESP] Arriel, Nair Helena de Castro Costa, Marcelo Marchi [UNESP] Trevisoli, Sandra Helena Uneda Di Mauro, Sonia Marli Zingaretti [UNESP] |
| dc.subject.por.fl_str_mv |
Conditions, watermelon, sesame DNA amplification Citrullus lanatus var. lanatus Sesamum indicum |
| topic |
Conditions, watermelon, sesame DNA amplification Citrullus lanatus var. lanatus Sesamum indicum |
| description |
The Random Amplified Polymorphic DNA (RAPD) technique is powerful for DNA polymorphism determinations and is widely used in research involving different organisms, but it is known that RAPD can be affected by many factors that may result in false positive bands and non-reproducible assays. In this study, we analyzed the effect of several factors such as DNA template, primer and Taq DNA polymerase concentrations to optimize and standardize the RAPD technique for further genetic studies with Citrulus lanattus and Sesamum indicum L. The best combination of DNA, Taq DNA polymerase enzyme and primer concentrations in RAPD amplification procedures for sesame and watermelon genotypes was established. |
| publishDate |
2006 |
| dc.date.none.fl_str_mv |
2006-12-01 2014-05-27T11:22:03Z 2014-05-27T11:22:03Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.12702/1984-7033.v06n02a08 Crop Breeding and Applied Biotechnology, v. 6, n. 2, p. 167-170, 2006. 1518-7853 http://hdl.handle.net/11449/69276 10.12702/1984-7033.v06n02a08 2-s2.0-41149127363 2-s2.0-41149127363.pdf 1275652518822095 |
| url |
http://dx.doi.org/10.12702/1984-7033.v06n02a08 http://hdl.handle.net/11449/69276 |
| identifier_str_mv |
Crop Breeding and Applied Biotechnology, v. 6, n. 2, p. 167-170, 2006. 1518-7853 10.12702/1984-7033.v06n02a08 2-s2.0-41149127363 2-s2.0-41149127363.pdf 1275652518822095 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Crop Breeding and Applied Biotechnology 0,609 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
167-170 application/pdf |
| dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
| instname_str |
Universidade Estadual Paulista (UNESP) |
| instacron_str |
UNESP |
| institution |
UNESP |
| reponame_str |
Repositório Institucional da UNESP |
| collection |
Repositório Institucional da UNESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
| repository.mail.fl_str_mv |
|
| _version_ |
1803650183642218496 |