Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil

Detalhes bibliográficos
Autor(a) principal: Franco, Marília Masello Junqueira [UNESP]
Data de Publicação: 2013
Outros Autores: Paes, Antonio Carlos [UNESP], Ribeiro, Márcio Garcia [UNESP], de Figueiredo Pantoja, José Carlos [UNESP], Santos, Adolfo Carlos Barreto [UNESP], Miyata, Marcelo [UNESP], Leite, Clarice Queico Fujimura [UNESP], Motta, Rodrigo Garcia [UNESP], Listoni, Fernando José Paganini [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
DOI: 10.1186/1746-6148-9-85
Texto Completo: http://dx.doi.org/10.1186/1746-6148-9-85
http://hdl.handle.net/11449/75165
Resumo: Background: Mycobacterium spp. is one of the most important species of zoonotic pathogens that can be transmitted from cattle to humans. The presence of these opportunistic, pathogenic bacteria in bovine milk has emerged as a public-health concern, especially among individuals who consume raw milk and related dairy products. To address this concern, the Brazilian control and eradication program focusing on bovine tuberculosis, was established in 2001. However, bovine tuberculosis continues to afflict approximately 1,3 percent of the cattle in Brazil. In the present study, 300 samples of milk from bovine herds, obtained from both individual and collective bulk tanks and informal points of sale, were cultured on Löwenstein-Jensen and Stonebrink media. Polymerase chain reaction (PCR)-based tests and restriction-enzyme pattern analysis were then performed on the colonies exhibiting phenotypes suggestive of Mycobacterium spp., which were characterized as acid-fast bacilli.Results: Of the 300 bovine milk samples that were processed, 24 were positively identified as Mycobacterium spp.Molecular identification detected 15 unique mycobacterial species: Mycobacterium bovis, M. gordonae, M. fortuitum, M. intracellulare, M. flavescens, M. duvalii, M. haemophilum, M. immunogenum, M. lentiflavum, M. mucogenicum, M. novocastrense, M. parafortuitum, M. smegmatis, M. terrae and M. vaccae. The isolation of bacteria from the various locations occurred in the following proportions: 9 percent of the individual bulk-tank samples, 7 percent of the collective bulk-tank samples and 8 percent of the informal-trade samples. No statistically significant difference was observed between the presence of Mycobacterium spp. in the three types of samples collected, the milk production profiles, the presence of veterinary assistance and the reported concerns about bovine tuberculosis prevention in the herds.Conclusion: The microbiological cultures associated with PCR-based identification tests are possible tools for the investigation of the presence of Mycobacterium spp. in milk samples. Using these methods, we found that the Brazilian population may be regularly exposed to mycobacteria by consuming raw bovine milk and related dairy products. These evidences reinforces the need to optimize quality programs of dairy products, to intensify the sanitary inspection of these products and the necessity of further studies on the presence of Mycobacterium spp. in milk and milk-based products. © 2013 Franco et al.; licensee BioMed Central Ltd.
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spelling Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, BrazilBovineBrazilBulk tanksMycobacterium sppPublic healthRaw milkBacteria (microorganisms)BosBovinaeCorynebacterineaeMycobacteriumMycobacterium bovisMycobacterium duvaliiMycobacterium flavescensMycobacterium fortuitumMycobacterium gordonaeMycobacterium haemophilumMycobacterium immunogenumMycobacterium intracellulareMycobacterium lentiflavumMycobacterium mucogenicumMycobacterium novocastrenseMycobacterium parafortuitumMycobacterium smegmatisMycobacterium terraeMycobacterium vaccaeBackground: Mycobacterium spp. is one of the most important species of zoonotic pathogens that can be transmitted from cattle to humans. The presence of these opportunistic, pathogenic bacteria in bovine milk has emerged as a public-health concern, especially among individuals who consume raw milk and related dairy products. To address this concern, the Brazilian control and eradication program focusing on bovine tuberculosis, was established in 2001. However, bovine tuberculosis continues to afflict approximately 1,3 percent of the cattle in Brazil. In the present study, 300 samples of milk from bovine herds, obtained from both individual and collective bulk tanks and informal points of sale, were cultured on Löwenstein-Jensen and Stonebrink media. Polymerase chain reaction (PCR)-based tests and restriction-enzyme pattern analysis were then performed on the colonies exhibiting phenotypes suggestive of Mycobacterium spp., which were characterized as acid-fast bacilli.Results: Of the 300 bovine milk samples that were processed, 24 were positively identified as Mycobacterium spp.Molecular identification detected 15 unique mycobacterial species: Mycobacterium bovis, M. gordonae, M. fortuitum, M. intracellulare, M. flavescens, M. duvalii, M. haemophilum, M. immunogenum, M. lentiflavum, M. mucogenicum, M. novocastrense, M. parafortuitum, M. smegmatis, M. terrae and M. vaccae. The isolation of bacteria from the various locations occurred in the following proportions: 9 percent of the individual bulk-tank samples, 7 percent of the collective bulk-tank samples and 8 percent of the informal-trade samples. No statistically significant difference was observed between the presence of Mycobacterium spp. in the three types of samples collected, the milk production profiles, the presence of veterinary assistance and the reported concerns about bovine tuberculosis prevention in the herds.Conclusion: The microbiological cultures associated with PCR-based identification tests are possible tools for the investigation of the presence of Mycobacterium spp. in milk samples. Using these methods, we found that the Brazilian population may be regularly exposed to mycobacteria by consuming raw bovine milk and related dairy products. These evidences reinforces the need to optimize quality programs of dairy products, to intensify the sanitary inspection of these products and the necessity of further studies on the presence of Mycobacterium spp. in milk and milk-based products. © 2013 Franco et al.; licensee BioMed Central Ltd.Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Science Universidade Estadual Paulista (UNESP), Box 56018618-970, Botucatu, State of Sao PauloLaboratory of Micobacteriology School of Pharmacy Sciences Universidade Estadual Paulista (UNESP), 14800-901, Araraquara, State of Sao PauloDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Science Universidade Estadual Paulista (UNESP), Box 56018618-970, Botucatu, State of Sao PauloLaboratory of Micobacteriology School of Pharmacy Sciences Universidade Estadual Paulista (UNESP), 14800-901, Araraquara, State of Sao PauloUniversidade Estadual Paulista (Unesp)Franco, Marília Masello Junqueira [UNESP]Paes, Antonio Carlos [UNESP]Ribeiro, Márcio Garcia [UNESP]de Figueiredo Pantoja, José Carlos [UNESP]Santos, Adolfo Carlos Barreto [UNESP]Miyata, Marcelo [UNESP]Leite, Clarice Queico Fujimura [UNESP]Motta, Rodrigo Garcia [UNESP]Listoni, Fernando José Paganini [UNESP]2014-05-27T11:28:59Z2014-05-27T11:28:59Z2013-04-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/1746-6148-9-85BMC Veterinary Research, v. 9.1746-6148http://hdl.handle.net/11449/7516510.1186/1746-6148-9-85WOS:0003186602000012-s2.0-848766765322-s2.0-84876676532.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Veterinary Research1.9580,934info:eu-repo/semantics/openAccess2024-09-03T14:12:03Zoai:repositorio.unesp.br:11449/75165Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:12:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil
title Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil
spellingShingle Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil
Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil
Franco, Marília Masello Junqueira [UNESP]
Bovine
Brazil
Bulk tanks
Mycobacterium spp
Public health
Raw milk
Bacteria (microorganisms)
Bos
Bovinae
Corynebacterineae
Mycobacterium
Mycobacterium bovis
Mycobacterium duvalii
Mycobacterium flavescens
Mycobacterium fortuitum
Mycobacterium gordonae
Mycobacterium haemophilum
Mycobacterium immunogenum
Mycobacterium intracellulare
Mycobacterium lentiflavum
Mycobacterium mucogenicum
Mycobacterium novocastrense
Mycobacterium parafortuitum
Mycobacterium smegmatis
Mycobacterium terrae
Mycobacterium vaccae
Franco, Marília Masello Junqueira [UNESP]
Bovine
Brazil
Bulk tanks
Mycobacterium spp
Public health
Raw milk
Bacteria (microorganisms)
Bos
Bovinae
Corynebacterineae
Mycobacterium
Mycobacterium bovis
Mycobacterium duvalii
Mycobacterium flavescens
Mycobacterium fortuitum
Mycobacterium gordonae
Mycobacterium haemophilum
Mycobacterium immunogenum
Mycobacterium intracellulare
Mycobacterium lentiflavum
Mycobacterium mucogenicum
Mycobacterium novocastrense
Mycobacterium parafortuitum
Mycobacterium smegmatis
Mycobacterium terrae
Mycobacterium vaccae
title_short Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil
title_full Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil
title_fullStr Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil
Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil
title_full_unstemmed Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil
Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil
title_sort Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil
author Franco, Marília Masello Junqueira [UNESP]
author_facet Franco, Marília Masello Junqueira [UNESP]
Franco, Marília Masello Junqueira [UNESP]
Paes, Antonio Carlos [UNESP]
Ribeiro, Márcio Garcia [UNESP]
de Figueiredo Pantoja, José Carlos [UNESP]
Santos, Adolfo Carlos Barreto [UNESP]
Miyata, Marcelo [UNESP]
Leite, Clarice Queico Fujimura [UNESP]
Motta, Rodrigo Garcia [UNESP]
Listoni, Fernando José Paganini [UNESP]
Paes, Antonio Carlos [UNESP]
Ribeiro, Márcio Garcia [UNESP]
de Figueiredo Pantoja, José Carlos [UNESP]
Santos, Adolfo Carlos Barreto [UNESP]
Miyata, Marcelo [UNESP]
Leite, Clarice Queico Fujimura [UNESP]
Motta, Rodrigo Garcia [UNESP]
Listoni, Fernando José Paganini [UNESP]
author_role author
author2 Paes, Antonio Carlos [UNESP]
Ribeiro, Márcio Garcia [UNESP]
de Figueiredo Pantoja, José Carlos [UNESP]
Santos, Adolfo Carlos Barreto [UNESP]
Miyata, Marcelo [UNESP]
Leite, Clarice Queico Fujimura [UNESP]
Motta, Rodrigo Garcia [UNESP]
Listoni, Fernando José Paganini [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Franco, Marília Masello Junqueira [UNESP]
Paes, Antonio Carlos [UNESP]
Ribeiro, Márcio Garcia [UNESP]
de Figueiredo Pantoja, José Carlos [UNESP]
Santos, Adolfo Carlos Barreto [UNESP]
Miyata, Marcelo [UNESP]
Leite, Clarice Queico Fujimura [UNESP]
Motta, Rodrigo Garcia [UNESP]
Listoni, Fernando José Paganini [UNESP]
dc.subject.por.fl_str_mv Bovine
Brazil
Bulk tanks
Mycobacterium spp
Public health
Raw milk
Bacteria (microorganisms)
Bos
Bovinae
Corynebacterineae
Mycobacterium
Mycobacterium bovis
Mycobacterium duvalii
Mycobacterium flavescens
Mycobacterium fortuitum
Mycobacterium gordonae
Mycobacterium haemophilum
Mycobacterium immunogenum
Mycobacterium intracellulare
Mycobacterium lentiflavum
Mycobacterium mucogenicum
Mycobacterium novocastrense
Mycobacterium parafortuitum
Mycobacterium smegmatis
Mycobacterium terrae
Mycobacterium vaccae
topic Bovine
Brazil
Bulk tanks
Mycobacterium spp
Public health
Raw milk
Bacteria (microorganisms)
Bos
Bovinae
Corynebacterineae
Mycobacterium
Mycobacterium bovis
Mycobacterium duvalii
Mycobacterium flavescens
Mycobacterium fortuitum
Mycobacterium gordonae
Mycobacterium haemophilum
Mycobacterium immunogenum
Mycobacterium intracellulare
Mycobacterium lentiflavum
Mycobacterium mucogenicum
Mycobacterium novocastrense
Mycobacterium parafortuitum
Mycobacterium smegmatis
Mycobacterium terrae
Mycobacterium vaccae
description Background: Mycobacterium spp. is one of the most important species of zoonotic pathogens that can be transmitted from cattle to humans. The presence of these opportunistic, pathogenic bacteria in bovine milk has emerged as a public-health concern, especially among individuals who consume raw milk and related dairy products. To address this concern, the Brazilian control and eradication program focusing on bovine tuberculosis, was established in 2001. However, bovine tuberculosis continues to afflict approximately 1,3 percent of the cattle in Brazil. In the present study, 300 samples of milk from bovine herds, obtained from both individual and collective bulk tanks and informal points of sale, were cultured on Löwenstein-Jensen and Stonebrink media. Polymerase chain reaction (PCR)-based tests and restriction-enzyme pattern analysis were then performed on the colonies exhibiting phenotypes suggestive of Mycobacterium spp., which were characterized as acid-fast bacilli.Results: Of the 300 bovine milk samples that were processed, 24 were positively identified as Mycobacterium spp.Molecular identification detected 15 unique mycobacterial species: Mycobacterium bovis, M. gordonae, M. fortuitum, M. intracellulare, M. flavescens, M. duvalii, M. haemophilum, M. immunogenum, M. lentiflavum, M. mucogenicum, M. novocastrense, M. parafortuitum, M. smegmatis, M. terrae and M. vaccae. The isolation of bacteria from the various locations occurred in the following proportions: 9 percent of the individual bulk-tank samples, 7 percent of the collective bulk-tank samples and 8 percent of the informal-trade samples. No statistically significant difference was observed between the presence of Mycobacterium spp. in the three types of samples collected, the milk production profiles, the presence of veterinary assistance and the reported concerns about bovine tuberculosis prevention in the herds.Conclusion: The microbiological cultures associated with PCR-based identification tests are possible tools for the investigation of the presence of Mycobacterium spp. in milk samples. Using these methods, we found that the Brazilian population may be regularly exposed to mycobacteria by consuming raw bovine milk and related dairy products. These evidences reinforces the need to optimize quality programs of dairy products, to intensify the sanitary inspection of these products and the necessity of further studies on the presence of Mycobacterium spp. in milk and milk-based products. © 2013 Franco et al.; licensee BioMed Central Ltd.
publishDate 2013
dc.date.none.fl_str_mv 2013-04-24
2014-05-27T11:28:59Z
2014-05-27T11:28:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/1746-6148-9-85
BMC Veterinary Research, v. 9.
1746-6148
http://hdl.handle.net/11449/75165
10.1186/1746-6148-9-85
WOS:000318660200001
2-s2.0-84876676532
2-s2.0-84876676532.pdf
url http://dx.doi.org/10.1186/1746-6148-9-85
http://hdl.handle.net/11449/75165
identifier_str_mv BMC Veterinary Research, v. 9.
1746-6148
10.1186/1746-6148-9-85
WOS:000318660200001
2-s2.0-84876676532
2-s2.0-84876676532.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv BMC Veterinary Research
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reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
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institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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dc.identifier.doi.none.fl_str_mv 10.1186/1746-6148-9-85