Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00424-016-1793-x http://hdl.handle.net/11449/172858 |
Resumo: | The orexins are hypothalamic neuropeptides involved in an array of functions such as regulation of sleep/wake states and chemoreception to CO2/pH. The locus coeruleus (LC) is a chemosensitive site and expresses an extensive population of orexin receptor 1 (OX1R). We tested the hypothesis that OX1Rs located in the LC participate in the ventilatory response to hypercapnia in a vigilance state and diurnal cycle-dependent manner. For this, we performed unilateral injections of SB-334867 (OX1R antagonist, 5 mM) into the LC of male Wistar rats and evaluated the ventilatory response to 7 % CO2 during wakefulness and sleep in the dark and light phases of the diurnal cycle. Hypercapnia induced an increase in ventilation (VE) in all groups compared to normocapnic values. However, during the dark phase, but not in the light phase, SB-334867 injection promoted an attenuation of the hypercapnic chemoreflex during wakefulness (VE: vehicle, 1502.6 ± 100 mL kg−1 min−1 vs SB-334867, 1200.3 ± 70.0 mL kg−1 min−1) but not during sleep (VE: vehicle, 1383.0 ± 113.9 vs SB-334687, 1287.6 ± 92.1 mL kg−1 min−1), due to changes in tidal volume (VT). We suggest that projections of orexin-containing neurons to the LC contribute, via OX1Rs, to the hypercapnic chemoreflex during wakefulness in the dark phase. |
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Orexinergic system in the locus coeruleus modulates the CO2 ventilatory responseAwakeChemoreceptionHypercapniaOrexinSleepVentilationThe orexins are hypothalamic neuropeptides involved in an array of functions such as regulation of sleep/wake states and chemoreception to CO2/pH. The locus coeruleus (LC) is a chemosensitive site and expresses an extensive population of orexin receptor 1 (OX1R). We tested the hypothesis that OX1Rs located in the LC participate in the ventilatory response to hypercapnia in a vigilance state and diurnal cycle-dependent manner. For this, we performed unilateral injections of SB-334867 (OX1R antagonist, 5 mM) into the LC of male Wistar rats and evaluated the ventilatory response to 7 % CO2 during wakefulness and sleep in the dark and light phases of the diurnal cycle. Hypercapnia induced an increase in ventilation (VE) in all groups compared to normocapnic values. However, during the dark phase, but not in the light phase, SB-334867 injection promoted an attenuation of the hypercapnic chemoreflex during wakefulness (VE: vehicle, 1502.6 ± 100 mL kg−1 min−1 vs SB-334867, 1200.3 ± 70.0 mL kg−1 min−1) but not during sleep (VE: vehicle, 1383.0 ± 113.9 vs SB-334687, 1287.6 ± 92.1 mL kg−1 min−1), due to changes in tidal volume (VT). We suggest that projections of orexin-containing neurons to the LC contribute, via OX1Rs, to the hypercapnic chemoreflex during wakefulness in the dark phase.Department of Animal Morphology and Physiology Sao Paulo State University—UNESP/FCAV at JaboticabalDepartment of Physiology Institute of Bioscience São Paulo State University—UNESPDepartment of Animal Morphology and Physiology Sao Paulo State University—UNESP/FCAV at JaboticabalDepartment of Physiology Institute of Bioscience São Paulo State University—UNESPUniversidade Estadual Paulista (Unesp)Vicente, Mariane C. [UNESP]Dias, Mirela B. [UNESP]Fonseca, Elisa M. [UNESP]Bícego, Kênia C. [UNESP]Gargaglioni, Luciane H. [UNESP]2018-12-11T17:02:27Z2018-12-11T17:02:27Z2016-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article763-774application/pdfhttp://dx.doi.org/10.1007/s00424-016-1793-xPflugers Archiv European Journal of Physiology, v. 468, n. 5, p. 763-774, 2016.1432-20130031-6768http://hdl.handle.net/11449/17285810.1007/s00424-016-1793-x2-s2.0-849642031282-s2.0-84964203128.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPflugers Archiv European Journal of Physiology1,4791,479info:eu-repo/semantics/openAccess2024-06-06T18:42:28Zoai:repositorio.unesp.br:11449/172858Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:26:48.807125Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response |
title |
Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response |
spellingShingle |
Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response Vicente, Mariane C. [UNESP] Awake Chemoreception Hypercapnia Orexin Sleep Ventilation |
title_short |
Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response |
title_full |
Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response |
title_fullStr |
Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response |
title_full_unstemmed |
Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response |
title_sort |
Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response |
author |
Vicente, Mariane C. [UNESP] |
author_facet |
Vicente, Mariane C. [UNESP] Dias, Mirela B. [UNESP] Fonseca, Elisa M. [UNESP] Bícego, Kênia C. [UNESP] Gargaglioni, Luciane H. [UNESP] |
author_role |
author |
author2 |
Dias, Mirela B. [UNESP] Fonseca, Elisa M. [UNESP] Bícego, Kênia C. [UNESP] Gargaglioni, Luciane H. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Vicente, Mariane C. [UNESP] Dias, Mirela B. [UNESP] Fonseca, Elisa M. [UNESP] Bícego, Kênia C. [UNESP] Gargaglioni, Luciane H. [UNESP] |
dc.subject.por.fl_str_mv |
Awake Chemoreception Hypercapnia Orexin Sleep Ventilation |
topic |
Awake Chemoreception Hypercapnia Orexin Sleep Ventilation |
description |
The orexins are hypothalamic neuropeptides involved in an array of functions such as regulation of sleep/wake states and chemoreception to CO2/pH. The locus coeruleus (LC) is a chemosensitive site and expresses an extensive population of orexin receptor 1 (OX1R). We tested the hypothesis that OX1Rs located in the LC participate in the ventilatory response to hypercapnia in a vigilance state and diurnal cycle-dependent manner. For this, we performed unilateral injections of SB-334867 (OX1R antagonist, 5 mM) into the LC of male Wistar rats and evaluated the ventilatory response to 7 % CO2 during wakefulness and sleep in the dark and light phases of the diurnal cycle. Hypercapnia induced an increase in ventilation (VE) in all groups compared to normocapnic values. However, during the dark phase, but not in the light phase, SB-334867 injection promoted an attenuation of the hypercapnic chemoreflex during wakefulness (VE: vehicle, 1502.6 ± 100 mL kg−1 min−1 vs SB-334867, 1200.3 ± 70.0 mL kg−1 min−1) but not during sleep (VE: vehicle, 1383.0 ± 113.9 vs SB-334687, 1287.6 ± 92.1 mL kg−1 min−1), due to changes in tidal volume (VT). We suggest that projections of orexin-containing neurons to the LC contribute, via OX1Rs, to the hypercapnic chemoreflex during wakefulness in the dark phase. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-05-01 2018-12-11T17:02:27Z 2018-12-11T17:02:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00424-016-1793-x Pflugers Archiv European Journal of Physiology, v. 468, n. 5, p. 763-774, 2016. 1432-2013 0031-6768 http://hdl.handle.net/11449/172858 10.1007/s00424-016-1793-x 2-s2.0-84964203128 2-s2.0-84964203128.pdf |
url |
http://dx.doi.org/10.1007/s00424-016-1793-x http://hdl.handle.net/11449/172858 |
identifier_str_mv |
Pflugers Archiv European Journal of Physiology, v. 468, n. 5, p. 763-774, 2016. 1432-2013 0031-6768 10.1007/s00424-016-1793-x 2-s2.0-84964203128 2-s2.0-84964203128.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pflugers Archiv European Journal of Physiology 1,479 1,479 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
763-774 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129521926275072 |