Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response

Detalhes bibliográficos
Autor(a) principal: Vicente, Mariane C. [UNESP]
Data de Publicação: 2016
Outros Autores: Dias, Mirela B. [UNESP], Fonseca, Elisa M. [UNESP], Bícego, Kênia C. [UNESP], Gargaglioni, Luciane H. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00424-016-1793-x
http://hdl.handle.net/11449/172858
Resumo: The orexins are hypothalamic neuropeptides involved in an array of functions such as regulation of sleep/wake states and chemoreception to CO2/pH. The locus coeruleus (LC) is a chemosensitive site and expresses an extensive population of orexin receptor 1 (OX1R). We tested the hypothesis that OX1Rs located in the LC participate in the ventilatory response to hypercapnia in a vigilance state and diurnal cycle-dependent manner. For this, we performed unilateral injections of SB-334867 (OX1R antagonist, 5 mM) into the LC of male Wistar rats and evaluated the ventilatory response to 7 % CO2 during wakefulness and sleep in the dark and light phases of the diurnal cycle. Hypercapnia induced an increase in ventilation (VE) in all groups compared to normocapnic values. However, during the dark phase, but not in the light phase, SB-334867 injection promoted an attenuation of the hypercapnic chemoreflex during wakefulness (VE: vehicle, 1502.6 ± 100 mL kg−1 min−1 vs SB-334867, 1200.3 ± 70.0 mL kg−1 min−1) but not during sleep (VE: vehicle, 1383.0 ± 113.9 vs SB-334687, 1287.6 ± 92.1 mL kg−1 min−1), due to changes in tidal volume (VT). We suggest that projections of orexin-containing neurons to the LC contribute, via OX1Rs, to the hypercapnic chemoreflex during wakefulness in the dark phase.
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spelling Orexinergic system in the locus coeruleus modulates the CO2 ventilatory responseAwakeChemoreceptionHypercapniaOrexinSleepVentilationThe orexins are hypothalamic neuropeptides involved in an array of functions such as regulation of sleep/wake states and chemoreception to CO2/pH. The locus coeruleus (LC) is a chemosensitive site and expresses an extensive population of orexin receptor 1 (OX1R). We tested the hypothesis that OX1Rs located in the LC participate in the ventilatory response to hypercapnia in a vigilance state and diurnal cycle-dependent manner. For this, we performed unilateral injections of SB-334867 (OX1R antagonist, 5 mM) into the LC of male Wistar rats and evaluated the ventilatory response to 7 % CO2 during wakefulness and sleep in the dark and light phases of the diurnal cycle. Hypercapnia induced an increase in ventilation (VE) in all groups compared to normocapnic values. However, during the dark phase, but not in the light phase, SB-334867 injection promoted an attenuation of the hypercapnic chemoreflex during wakefulness (VE: vehicle, 1502.6 ± 100 mL kg−1 min−1 vs SB-334867, 1200.3 ± 70.0 mL kg−1 min−1) but not during sleep (VE: vehicle, 1383.0 ± 113.9 vs SB-334687, 1287.6 ± 92.1 mL kg−1 min−1), due to changes in tidal volume (VT). We suggest that projections of orexin-containing neurons to the LC contribute, via OX1Rs, to the hypercapnic chemoreflex during wakefulness in the dark phase.Department of Animal Morphology and Physiology Sao Paulo State University—UNESP/FCAV at JaboticabalDepartment of Physiology Institute of Bioscience São Paulo State University—UNESPDepartment of Animal Morphology and Physiology Sao Paulo State University—UNESP/FCAV at JaboticabalDepartment of Physiology Institute of Bioscience São Paulo State University—UNESPUniversidade Estadual Paulista (Unesp)Vicente, Mariane C. [UNESP]Dias, Mirela B. [UNESP]Fonseca, Elisa M. [UNESP]Bícego, Kênia C. [UNESP]Gargaglioni, Luciane H. [UNESP]2018-12-11T17:02:27Z2018-12-11T17:02:27Z2016-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article763-774application/pdfhttp://dx.doi.org/10.1007/s00424-016-1793-xPflugers Archiv European Journal of Physiology, v. 468, n. 5, p. 763-774, 2016.1432-20130031-6768http://hdl.handle.net/11449/17285810.1007/s00424-016-1793-x2-s2.0-849642031282-s2.0-84964203128.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPflugers Archiv European Journal of Physiology1,4791,479info:eu-repo/semantics/openAccess2024-06-06T18:42:28Zoai:repositorio.unesp.br:11449/172858Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:26:48.807125Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response
title Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response
spellingShingle Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response
Vicente, Mariane C. [UNESP]
Awake
Chemoreception
Hypercapnia
Orexin
Sleep
Ventilation
title_short Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response
title_full Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response
title_fullStr Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response
title_full_unstemmed Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response
title_sort Orexinergic system in the locus coeruleus modulates the CO2 ventilatory response
author Vicente, Mariane C. [UNESP]
author_facet Vicente, Mariane C. [UNESP]
Dias, Mirela B. [UNESP]
Fonseca, Elisa M. [UNESP]
Bícego, Kênia C. [UNESP]
Gargaglioni, Luciane H. [UNESP]
author_role author
author2 Dias, Mirela B. [UNESP]
Fonseca, Elisa M. [UNESP]
Bícego, Kênia C. [UNESP]
Gargaglioni, Luciane H. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Vicente, Mariane C. [UNESP]
Dias, Mirela B. [UNESP]
Fonseca, Elisa M. [UNESP]
Bícego, Kênia C. [UNESP]
Gargaglioni, Luciane H. [UNESP]
dc.subject.por.fl_str_mv Awake
Chemoreception
Hypercapnia
Orexin
Sleep
Ventilation
topic Awake
Chemoreception
Hypercapnia
Orexin
Sleep
Ventilation
description The orexins are hypothalamic neuropeptides involved in an array of functions such as regulation of sleep/wake states and chemoreception to CO2/pH. The locus coeruleus (LC) is a chemosensitive site and expresses an extensive population of orexin receptor 1 (OX1R). We tested the hypothesis that OX1Rs located in the LC participate in the ventilatory response to hypercapnia in a vigilance state and diurnal cycle-dependent manner. For this, we performed unilateral injections of SB-334867 (OX1R antagonist, 5 mM) into the LC of male Wistar rats and evaluated the ventilatory response to 7 % CO2 during wakefulness and sleep in the dark and light phases of the diurnal cycle. Hypercapnia induced an increase in ventilation (VE) in all groups compared to normocapnic values. However, during the dark phase, but not in the light phase, SB-334867 injection promoted an attenuation of the hypercapnic chemoreflex during wakefulness (VE: vehicle, 1502.6 ± 100 mL kg−1 min−1 vs SB-334867, 1200.3 ± 70.0 mL kg−1 min−1) but not during sleep (VE: vehicle, 1383.0 ± 113.9 vs SB-334687, 1287.6 ± 92.1 mL kg−1 min−1), due to changes in tidal volume (VT). We suggest that projections of orexin-containing neurons to the LC contribute, via OX1Rs, to the hypercapnic chemoreflex during wakefulness in the dark phase.
publishDate 2016
dc.date.none.fl_str_mv 2016-05-01
2018-12-11T17:02:27Z
2018-12-11T17:02:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00424-016-1793-x
Pflugers Archiv European Journal of Physiology, v. 468, n. 5, p. 763-774, 2016.
1432-2013
0031-6768
http://hdl.handle.net/11449/172858
10.1007/s00424-016-1793-x
2-s2.0-84964203128
2-s2.0-84964203128.pdf
url http://dx.doi.org/10.1007/s00424-016-1793-x
http://hdl.handle.net/11449/172858
identifier_str_mv Pflugers Archiv European Journal of Physiology, v. 468, n. 5, p. 763-774, 2016.
1432-2013
0031-6768
10.1007/s00424-016-1793-x
2-s2.0-84964203128
2-s2.0-84964203128.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pflugers Archiv European Journal of Physiology
1,479
1,479
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 763-774
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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