Partial purification and some properties of a T2 ribonuclease from Aspergillus flavipes

Detalhes bibliográficos
Autor(a) principal: Gomes, Eleni [UNESP]
Data de Publicação: 1996
Outros Autores: Serzëdello, Alcides [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/224044
Resumo: A ribonuclease was partially purified from the culture medium of Aspergillus flavipes (IZ:1501), after 96 h of cultivation by chromatography on DEAE-cellulose and Sephadex G100 columns. The molecular weight of the RNase was estimated to be 40 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 50-55°C, respectively. Catalytic activity was inhibited by Zn+2, Fe+3, Hg+2 and Ag+ ions. The enzyme did not show an exact base specificity and produced four kinds of 3′-nucleotides from yeast RNA.
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spelling Partial purification and some properties of a T2 ribonuclease from Aspergillus flavipesAspergillusNucleotidesRibonucleaseA ribonuclease was partially purified from the culture medium of Aspergillus flavipes (IZ:1501), after 96 h of cultivation by chromatography on DEAE-cellulose and Sephadex G100 columns. The molecular weight of the RNase was estimated to be 40 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 50-55°C, respectively. Catalytic activity was inhibited by Zn+2, Fe+3, Hg+2 and Ag+ ions. The enzyme did not show an exact base specificity and produced four kinds of 3′-nucleotides from yeast RNA.Departamento de Biologia Inst. Biocencias, Letras Cie. Exatas Universidade Estadual Paulista, Sao Jose do Rio Preto, SPDepto. de Bioquim. e Microbiologia Instituto de Biocências Universidade Estadual Paulista, CEP 13506-900, Rio Claro, SPDepartamento de Biologia Inst. Biocencias, Letras Cie. Exatas Universidade Estadual Paulista, Sao Jose do Rio Preto, SPDepto. de Bioquim. e Microbiologia Instituto de Biocências Universidade Estadual Paulista, CEP 13506-900, Rio Claro, SPUniversidade Estadual Paulista (UNESP)Gomes, Eleni [UNESP]Serzëdello, Alcides [UNESP]2022-04-28T19:54:26Z2022-04-28T19:54:26Z1996-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article227-237Microbios, v. 87, n. 353, p. 227-237, 1996.0026-2633http://hdl.handle.net/11449/2240442-s2.0-0030361622Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobiosinfo:eu-repo/semantics/openAccess2022-04-28T19:54:26Zoai:repositorio.unesp.br:11449/224044Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:10:24.652625Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Partial purification and some properties of a T2 ribonuclease from Aspergillus flavipes
title Partial purification and some properties of a T2 ribonuclease from Aspergillus flavipes
spellingShingle Partial purification and some properties of a T2 ribonuclease from Aspergillus flavipes
Gomes, Eleni [UNESP]
Aspergillus
Nucleotides
Ribonuclease
title_short Partial purification and some properties of a T2 ribonuclease from Aspergillus flavipes
title_full Partial purification and some properties of a T2 ribonuclease from Aspergillus flavipes
title_fullStr Partial purification and some properties of a T2 ribonuclease from Aspergillus flavipes
title_full_unstemmed Partial purification and some properties of a T2 ribonuclease from Aspergillus flavipes
title_sort Partial purification and some properties of a T2 ribonuclease from Aspergillus flavipes
author Gomes, Eleni [UNESP]
author_facet Gomes, Eleni [UNESP]
Serzëdello, Alcides [UNESP]
author_role author
author2 Serzëdello, Alcides [UNESP]
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Gomes, Eleni [UNESP]
Serzëdello, Alcides [UNESP]
dc.subject.por.fl_str_mv Aspergillus
Nucleotides
Ribonuclease
topic Aspergillus
Nucleotides
Ribonuclease
description A ribonuclease was partially purified from the culture medium of Aspergillus flavipes (IZ:1501), after 96 h of cultivation by chromatography on DEAE-cellulose and Sephadex G100 columns. The molecular weight of the RNase was estimated to be 40 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 50-55°C, respectively. Catalytic activity was inhibited by Zn+2, Fe+3, Hg+2 and Ag+ ions. The enzyme did not show an exact base specificity and produced four kinds of 3′-nucleotides from yeast RNA.
publishDate 1996
dc.date.none.fl_str_mv 1996-12-01
2022-04-28T19:54:26Z
2022-04-28T19:54:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Microbios, v. 87, n. 353, p. 227-237, 1996.
0026-2633
http://hdl.handle.net/11449/224044
2-s2.0-0030361622
identifier_str_mv Microbios, v. 87, n. 353, p. 227-237, 1996.
0026-2633
2-s2.0-0030361622
url http://hdl.handle.net/11449/224044
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microbios
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 227-237
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129592468176896

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