Purification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ:1501)

Detalhes bibliográficos
Autor(a) principal: Gomes, Eleni [UNESP]
Data de Publicação: 1998
Outros Autores: Serzedello, Alcides [UNESP], Da Silva, Roberto [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/224099
Resumo: A RNase of Aspergillus flavlpes (IZ:1501) was purified from culture medium by chromatography on DEAE-cellulose and Sephadex G50 columns, after 96 h of cultivation. The molecular weight of the RNase was estimated to be 15 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 55°C, respectively. Catalytic activity was inhibited by Hg2+, Ag+, Fe3+, Co2+ and Zn2+. The enzyme showed guanosine specificity producing only 3′-GMP from yeast RNA.
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spelling Purification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ:1501)AspergillusNucleotidesRibonucleaseA RNase of Aspergillus flavlpes (IZ:1501) was purified from culture medium by chromatography on DEAE-cellulose and Sephadex G50 columns, after 96 h of cultivation. The molecular weight of the RNase was estimated to be 15 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 55°C, respectively. Catalytic activity was inhibited by Hg2+, Ag+, Fe3+, Co2+ and Zn2+. The enzyme showed guanosine specificity producing only 3′-GMP from yeast RNA.Lab. Bioquim. dos Processus M. Departamento de Biologia Univ. Estadual Paulista-IBILCE, CP 163, CEP 15054-000Depto. de Quim. e Geociências UNESP-IBILCEDepto. de Bioquim. e Microbiologia UNESP, Rio Claro, SPLab. Bioquim. dos Processus M. Departamento de Biologia Univ. Estadual Paulista-IBILCE, CP 163, CEP 15054-000Depto. de Quim. e Geociências UNESP-IBILCEDepto. de Bioquim. e Microbiologia UNESP, Rio Claro, SPUniversidade Estadual Paulista (UNESP)Gomes, Eleni [UNESP]Serzedello, Alcides [UNESP]Da Silva, Roberto [UNESP]2022-04-28T19:54:36Z2022-04-28T19:54:36Z1998-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article39-50Microbios, v. 96, n. 383, p. 39-50, 1998.0026-2633http://hdl.handle.net/11449/2240992-s2.0-0032262633Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobiosinfo:eu-repo/semantics/openAccess2022-04-28T19:54:36Zoai:repositorio.unesp.br:11449/224099Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:02:46.853239Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Purification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ:1501)
title Purification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ:1501)
spellingShingle Purification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ:1501)
Gomes, Eleni [UNESP]
Aspergillus
Nucleotides
Ribonuclease
title_short Purification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ:1501)
title_full Purification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ:1501)
title_fullStr Purification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ:1501)
title_full_unstemmed Purification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ:1501)
title_sort Purification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ:1501)
author Gomes, Eleni [UNESP]
author_facet Gomes, Eleni [UNESP]
Serzedello, Alcides [UNESP]
Da Silva, Roberto [UNESP]
author_role author
author2 Serzedello, Alcides [UNESP]
Da Silva, Roberto [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Gomes, Eleni [UNESP]
Serzedello, Alcides [UNESP]
Da Silva, Roberto [UNESP]
dc.subject.por.fl_str_mv Aspergillus
Nucleotides
Ribonuclease
topic Aspergillus
Nucleotides
Ribonuclease
description A RNase of Aspergillus flavlpes (IZ:1501) was purified from culture medium by chromatography on DEAE-cellulose and Sephadex G50 columns, after 96 h of cultivation. The molecular weight of the RNase was estimated to be 15 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 55°C, respectively. Catalytic activity was inhibited by Hg2+, Ag+, Fe3+, Co2+ and Zn2+. The enzyme showed guanosine specificity producing only 3′-GMP from yeast RNA.
publishDate 1998
dc.date.none.fl_str_mv 1998-12-01
2022-04-28T19:54:36Z
2022-04-28T19:54:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Microbios, v. 96, n. 383, p. 39-50, 1998.
0026-2633
http://hdl.handle.net/11449/224099
2-s2.0-0032262633
identifier_str_mv Microbios, v. 96, n. 383, p. 39-50, 1998.
0026-2633
2-s2.0-0032262633
url http://hdl.handle.net/11449/224099
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microbios
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 39-50
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128308193263616

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