Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua

Detalhes bibliográficos
Autor(a) principal: Lorençoni, Roselene Modolo Regueiro
Data de Publicação: 2015
Outros Autores: Pelai, Elisa Bizetti, Godoy, Moacir Fernandes, Dos Santos, Alice Cristina Antonio, Miyoshi, Lívia Hissae, De Oliveira, Rebeca Nicolás Paronetto, Padovani, Carlos Roberto, Fregonesi, Cristina Elena Prado Teles, Camargo Filho, José Carlos Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4067/S0717-95022015000200039
http://hdl.handle.net/11449/220427
Resumo: Physical effort stimulates an increase in oxygen consumption in tissues, generating toxic chemical species derived from oxygen (ROS), which are considered the initiators of the lipid peroxidation process (LPO), the major mechanism of cellular injury. As the essential mechanism for maintaining the electrolyte balance depends on an effective kidney function, oxidative stress in this organ can be a key factor in the development and persistence of hypertension. This study aimed to determine the kidney changes induced by a combination of fluid restriction and exercise in rats. The study consisted of 24 male Wistar rats of 90 days of age, divided into four groups, two of which were submitted to water restriction and exercise on a treadmill. Twenty-four hours after the last training session, the animals were euthanized and the left kidney was removed. The upper part of the kidney was used for the histological procedures and the lower part for the quantification of membrane lipoperoxides. Analysis of variance was applied after testing the normality of data, and the comparison between groups was performed using the Bonferroni test, adopting a significance of p<0.05. The restriction had an influence on body weight; kidney weight; proximal tubule maximum diameter; and area, perimeter and diameter of glomeruli, whereas the exercise affected the weight and the minimum diameter of the proximal tubule. According to the TBARS (thiobarbituric acid reactive substances) method, there was a difference between the G1 (control) and G3 (sedentary with water restriction) and between the G2 (exercised with water) and G4 (exercised with water restriction) compared to G1. The treadmill exercise combined with the water restriction promoted structural changes in the glomeruli and promoted oxidative stress, although neither variable corroborated for the potentiating of lipid peroxidation.
id UNSP_0c57a0ac6154bfc94937816b73b31e00
oai_identifier_str oai:repositorio.unesp.br:11449/220427
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de aguaHistology and renal lipoperoxidation in rats exercised on a treadmill and submitted to water restrictionKidneyMorphologyOxidative stressPhysical effort stimulates an increase in oxygen consumption in tissues, generating toxic chemical species derived from oxygen (ROS), which are considered the initiators of the lipid peroxidation process (LPO), the major mechanism of cellular injury. As the essential mechanism for maintaining the electrolyte balance depends on an effective kidney function, oxidative stress in this organ can be a key factor in the development and persistence of hypertension. This study aimed to determine the kidney changes induced by a combination of fluid restriction and exercise in rats. The study consisted of 24 male Wistar rats of 90 days of age, divided into four groups, two of which were submitted to water restriction and exercise on a treadmill. Twenty-four hours after the last training session, the animals were euthanized and the left kidney was removed. The upper part of the kidney was used for the histological procedures and the lower part for the quantification of membrane lipoperoxides. Analysis of variance was applied after testing the normality of data, and the comparison between groups was performed using the Bonferroni test, adopting a significance of p<0.05. The restriction had an influence on body weight; kidney weight; proximal tubule maximum diameter; and area, perimeter and diameter of glomeruli, whereas the exercise affected the weight and the minimum diameter of the proximal tubule. According to the TBARS (thiobarbituric acid reactive substances) method, there was a difference between the G1 (control) and G3 (sedentary with water restriction) and between the G2 (exercised with water) and G4 (exercised with water restriction) compared to G1. The treadmill exercise combined with the water restriction promoted structural changes in the glomeruli and promoted oxidative stress, although neither variable corroborated for the potentiating of lipid peroxidation.Physiotherapy Department at the University of Science and Technology FCT Universidad Estatal PaulistaPhysiotherapy from the University of Science and Technology FCT Universidad Estatal PaulistaUniversity of Science and Technology FCT Universidad Estatal PaulistaDepartment of Biostatistics Institute of Biosciences Universidad Estatal PaulistaUniversidad Estatal PaulistaLorençoni, Roselene Modolo RegueiroPelai, Elisa BizettiGodoy, Moacir FernandesDos Santos, Alice Cristina AntonioMiyoshi, Lívia HissaeDe Oliveira, Rebeca Nicolás ParonettoPadovani, Carlos RobertoFregonesi, Cristina Elena Prado TelesCamargo Filho, José Carlos Silva2022-04-28T19:01:29Z2022-04-28T19:01:29Z2015-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article660-665http://dx.doi.org/10.4067/S0717-95022015000200039International Journal of Morphology, v. 33, n. 2, p. 660-665, 2015.0717-95020717-9367http://hdl.handle.net/11449/22042710.4067/S0717-950220150002000392-s2.0-84939480064Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Morphologyinfo:eu-repo/semantics/openAccess2022-04-28T19:01:29Zoai:repositorio.unesp.br:11449/220427Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:01:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua
Histology and renal lipoperoxidation in rats exercised on a treadmill and submitted to water restriction
title Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua
spellingShingle Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua
Lorençoni, Roselene Modolo Regueiro
Kidney
Morphology
Oxidative stress
title_short Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua
title_full Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua
title_fullStr Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua
title_full_unstemmed Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua
title_sort Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua
author Lorençoni, Roselene Modolo Regueiro
author_facet Lorençoni, Roselene Modolo Regueiro
Pelai, Elisa Bizetti
Godoy, Moacir Fernandes
Dos Santos, Alice Cristina Antonio
Miyoshi, Lívia Hissae
De Oliveira, Rebeca Nicolás Paronetto
Padovani, Carlos Roberto
Fregonesi, Cristina Elena Prado Teles
Camargo Filho, José Carlos Silva
author_role author
author2 Pelai, Elisa Bizetti
Godoy, Moacir Fernandes
Dos Santos, Alice Cristina Antonio
Miyoshi, Lívia Hissae
De Oliveira, Rebeca Nicolás Paronetto
Padovani, Carlos Roberto
Fregonesi, Cristina Elena Prado Teles
Camargo Filho, José Carlos Silva
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Estatal Paulista
dc.contributor.author.fl_str_mv Lorençoni, Roselene Modolo Regueiro
Pelai, Elisa Bizetti
Godoy, Moacir Fernandes
Dos Santos, Alice Cristina Antonio
Miyoshi, Lívia Hissae
De Oliveira, Rebeca Nicolás Paronetto
Padovani, Carlos Roberto
Fregonesi, Cristina Elena Prado Teles
Camargo Filho, José Carlos Silva
dc.subject.por.fl_str_mv Kidney
Morphology
Oxidative stress
topic Kidney
Morphology
Oxidative stress
description Physical effort stimulates an increase in oxygen consumption in tissues, generating toxic chemical species derived from oxygen (ROS), which are considered the initiators of the lipid peroxidation process (LPO), the major mechanism of cellular injury. As the essential mechanism for maintaining the electrolyte balance depends on an effective kidney function, oxidative stress in this organ can be a key factor in the development and persistence of hypertension. This study aimed to determine the kidney changes induced by a combination of fluid restriction and exercise in rats. The study consisted of 24 male Wistar rats of 90 days of age, divided into four groups, two of which were submitted to water restriction and exercise on a treadmill. Twenty-four hours after the last training session, the animals were euthanized and the left kidney was removed. The upper part of the kidney was used for the histological procedures and the lower part for the quantification of membrane lipoperoxides. Analysis of variance was applied after testing the normality of data, and the comparison between groups was performed using the Bonferroni test, adopting a significance of p<0.05. The restriction had an influence on body weight; kidney weight; proximal tubule maximum diameter; and area, perimeter and diameter of glomeruli, whereas the exercise affected the weight and the minimum diameter of the proximal tubule. According to the TBARS (thiobarbituric acid reactive substances) method, there was a difference between the G1 (control) and G3 (sedentary with water restriction) and between the G2 (exercised with water) and G4 (exercised with water restriction) compared to G1. The treadmill exercise combined with the water restriction promoted structural changes in the glomeruli and promoted oxidative stress, although neither variable corroborated for the potentiating of lipid peroxidation.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
2022-04-28T19:01:29Z
2022-04-28T19:01:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4067/S0717-95022015000200039
International Journal of Morphology, v. 33, n. 2, p. 660-665, 2015.
0717-9502
0717-9367
http://hdl.handle.net/11449/220427
10.4067/S0717-95022015000200039
2-s2.0-84939480064
url http://dx.doi.org/10.4067/S0717-95022015000200039
http://hdl.handle.net/11449/220427
identifier_str_mv International Journal of Morphology, v. 33, n. 2, p. 660-665, 2015.
0717-9502
0717-9367
10.4067/S0717-95022015000200039
2-s2.0-84939480064
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Morphology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 660-665
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965296751017984