Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4067/S0717-95022015000200039 http://hdl.handle.net/11449/220427 |
Resumo: | Physical effort stimulates an increase in oxygen consumption in tissues, generating toxic chemical species derived from oxygen (ROS), which are considered the initiators of the lipid peroxidation process (LPO), the major mechanism of cellular injury. As the essential mechanism for maintaining the electrolyte balance depends on an effective kidney function, oxidative stress in this organ can be a key factor in the development and persistence of hypertension. This study aimed to determine the kidney changes induced by a combination of fluid restriction and exercise in rats. The study consisted of 24 male Wistar rats of 90 days of age, divided into four groups, two of which were submitted to water restriction and exercise on a treadmill. Twenty-four hours after the last training session, the animals were euthanized and the left kidney was removed. The upper part of the kidney was used for the histological procedures and the lower part for the quantification of membrane lipoperoxides. Analysis of variance was applied after testing the normality of data, and the comparison between groups was performed using the Bonferroni test, adopting a significance of p<0.05. The restriction had an influence on body weight; kidney weight; proximal tubule maximum diameter; and area, perimeter and diameter of glomeruli, whereas the exercise affected the weight and the minimum diameter of the proximal tubule. According to the TBARS (thiobarbituric acid reactive substances) method, there was a difference between the G1 (control) and G3 (sedentary with water restriction) and between the G2 (exercised with water) and G4 (exercised with water restriction) compared to G1. The treadmill exercise combined with the water restriction promoted structural changes in the glomeruli and promoted oxidative stress, although neither variable corroborated for the potentiating of lipid peroxidation. |
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Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de aguaHistology and renal lipoperoxidation in rats exercised on a treadmill and submitted to water restrictionKidneyMorphologyOxidative stressPhysical effort stimulates an increase in oxygen consumption in tissues, generating toxic chemical species derived from oxygen (ROS), which are considered the initiators of the lipid peroxidation process (LPO), the major mechanism of cellular injury. As the essential mechanism for maintaining the electrolyte balance depends on an effective kidney function, oxidative stress in this organ can be a key factor in the development and persistence of hypertension. This study aimed to determine the kidney changes induced by a combination of fluid restriction and exercise in rats. The study consisted of 24 male Wistar rats of 90 days of age, divided into four groups, two of which were submitted to water restriction and exercise on a treadmill. Twenty-four hours after the last training session, the animals were euthanized and the left kidney was removed. The upper part of the kidney was used for the histological procedures and the lower part for the quantification of membrane lipoperoxides. Analysis of variance was applied after testing the normality of data, and the comparison between groups was performed using the Bonferroni test, adopting a significance of p<0.05. The restriction had an influence on body weight; kidney weight; proximal tubule maximum diameter; and area, perimeter and diameter of glomeruli, whereas the exercise affected the weight and the minimum diameter of the proximal tubule. According to the TBARS (thiobarbituric acid reactive substances) method, there was a difference between the G1 (control) and G3 (sedentary with water restriction) and between the G2 (exercised with water) and G4 (exercised with water restriction) compared to G1. The treadmill exercise combined with the water restriction promoted structural changes in the glomeruli and promoted oxidative stress, although neither variable corroborated for the potentiating of lipid peroxidation.Physiotherapy Department at the University of Science and Technology FCT Universidad Estatal PaulistaPhysiotherapy from the University of Science and Technology FCT Universidad Estatal PaulistaUniversity of Science and Technology FCT Universidad Estatal PaulistaDepartment of Biostatistics Institute of Biosciences Universidad Estatal PaulistaUniversidad Estatal PaulistaLorençoni, Roselene Modolo RegueiroPelai, Elisa BizettiGodoy, Moacir FernandesDos Santos, Alice Cristina AntonioMiyoshi, Lívia HissaeDe Oliveira, Rebeca Nicolás ParonettoPadovani, Carlos RobertoFregonesi, Cristina Elena Prado TelesCamargo Filho, José Carlos Silva2022-04-28T19:01:29Z2022-04-28T19:01:29Z2015-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article660-665http://dx.doi.org/10.4067/S0717-95022015000200039International Journal of Morphology, v. 33, n. 2, p. 660-665, 2015.0717-95020717-9367http://hdl.handle.net/11449/22042710.4067/S0717-950220150002000392-s2.0-84939480064Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Morphologyinfo:eu-repo/semantics/openAccess2022-04-28T19:01:29Zoai:repositorio.unesp.br:11449/220427Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:25:42.964538Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua Histology and renal lipoperoxidation in rats exercised on a treadmill and submitted to water restriction |
title |
Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua |
spellingShingle |
Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua Lorençoni, Roselene Modolo Regueiro Kidney Morphology Oxidative stress |
title_short |
Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua |
title_full |
Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua |
title_fullStr |
Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua |
title_full_unstemmed |
Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua |
title_sort |
Histología y lipoperoxidación renal en ratas ejercitadas sobre una caminadora y sometidas a restricción de agua |
author |
Lorençoni, Roselene Modolo Regueiro |
author_facet |
Lorençoni, Roselene Modolo Regueiro Pelai, Elisa Bizetti Godoy, Moacir Fernandes Dos Santos, Alice Cristina Antonio Miyoshi, Lívia Hissae De Oliveira, Rebeca Nicolás Paronetto Padovani, Carlos Roberto Fregonesi, Cristina Elena Prado Teles Camargo Filho, José Carlos Silva |
author_role |
author |
author2 |
Pelai, Elisa Bizetti Godoy, Moacir Fernandes Dos Santos, Alice Cristina Antonio Miyoshi, Lívia Hissae De Oliveira, Rebeca Nicolás Paronetto Padovani, Carlos Roberto Fregonesi, Cristina Elena Prado Teles Camargo Filho, José Carlos Silva |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidad Estatal Paulista |
dc.contributor.author.fl_str_mv |
Lorençoni, Roselene Modolo Regueiro Pelai, Elisa Bizetti Godoy, Moacir Fernandes Dos Santos, Alice Cristina Antonio Miyoshi, Lívia Hissae De Oliveira, Rebeca Nicolás Paronetto Padovani, Carlos Roberto Fregonesi, Cristina Elena Prado Teles Camargo Filho, José Carlos Silva |
dc.subject.por.fl_str_mv |
Kidney Morphology Oxidative stress |
topic |
Kidney Morphology Oxidative stress |
description |
Physical effort stimulates an increase in oxygen consumption in tissues, generating toxic chemical species derived from oxygen (ROS), which are considered the initiators of the lipid peroxidation process (LPO), the major mechanism of cellular injury. As the essential mechanism for maintaining the electrolyte balance depends on an effective kidney function, oxidative stress in this organ can be a key factor in the development and persistence of hypertension. This study aimed to determine the kidney changes induced by a combination of fluid restriction and exercise in rats. The study consisted of 24 male Wistar rats of 90 days of age, divided into four groups, two of which were submitted to water restriction and exercise on a treadmill. Twenty-four hours after the last training session, the animals were euthanized and the left kidney was removed. The upper part of the kidney was used for the histological procedures and the lower part for the quantification of membrane lipoperoxides. Analysis of variance was applied after testing the normality of data, and the comparison between groups was performed using the Bonferroni test, adopting a significance of p<0.05. The restriction had an influence on body weight; kidney weight; proximal tubule maximum diameter; and area, perimeter and diameter of glomeruli, whereas the exercise affected the weight and the minimum diameter of the proximal tubule. According to the TBARS (thiobarbituric acid reactive substances) method, there was a difference between the G1 (control) and G3 (sedentary with water restriction) and between the G2 (exercised with water) and G4 (exercised with water restriction) compared to G1. The treadmill exercise combined with the water restriction promoted structural changes in the glomeruli and promoted oxidative stress, although neither variable corroborated for the potentiating of lipid peroxidation. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 2022-04-28T19:01:29Z 2022-04-28T19:01:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4067/S0717-95022015000200039 International Journal of Morphology, v. 33, n. 2, p. 660-665, 2015. 0717-9502 0717-9367 http://hdl.handle.net/11449/220427 10.4067/S0717-95022015000200039 2-s2.0-84939480064 |
url |
http://dx.doi.org/10.4067/S0717-95022015000200039 http://hdl.handle.net/11449/220427 |
identifier_str_mv |
International Journal of Morphology, v. 33, n. 2, p. 660-665, 2015. 0717-9502 0717-9367 10.4067/S0717-95022015000200039 2-s2.0-84939480064 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Morphology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
660-665 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129200367861760 |