Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1021/acs.jproteome.2c00652 http://hdl.handle.net/11449/248374 |
Resumo: | The use of saliva as a protein source prior to microbiological and biological assays requires previous processing. However, the effect of these processing methods on the proteomic profile of saliva has not been tested. Stimulated human saliva was collected from eight healthy volunteers. Non-processed saliva was compared with 0.22 μm filtered, 0.45 μm filtered, and pasteurized saliva, by liquid chromatography-mass spectrometry. Data are available via ProteomeXchange with identifier PXD039248. The effect of processed saliva on microbial adhesion was tested using bacterial and fungus species and in biological cell behavior using HaCaT immortalized human keratinocytes. Two hundred and seventy-eight proteins were identified in non-processed saliva, of which 54 proteins (≈19%) were exclusive. Saliva processing reduced identified proteins to 222 (≈80%) for the 0.22 μm group, 219 (≈79%) for the 0.45 μm group, and 201 (≈72%) for the pasteurized saliva, compared to non-processed saliva. The proteomic profile showed similar molecular functions and biological processes. The different saliva processing methods did not alter microbial adhesion (ANOVA, p > 0.05). Interestingly, pasteurized saliva reduced keratinocyte cell viability. Saliva processing methods tested reduced the proteomic profile diversity of saliva but maintained similar molecular functions and biological processes, not interfering with microbial adhesion and cell viability, except for pasteurization, which reduced cell viability. |
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Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processesmicrobiologyproteomicsalivasterilizationstudy modelThe use of saliva as a protein source prior to microbiological and biological assays requires previous processing. However, the effect of these processing methods on the proteomic profile of saliva has not been tested. Stimulated human saliva was collected from eight healthy volunteers. Non-processed saliva was compared with 0.22 μm filtered, 0.45 μm filtered, and pasteurized saliva, by liquid chromatography-mass spectrometry. Data are available via ProteomeXchange with identifier PXD039248. The effect of processed saliva on microbial adhesion was tested using bacterial and fungus species and in biological cell behavior using HaCaT immortalized human keratinocytes. Two hundred and seventy-eight proteins were identified in non-processed saliva, of which 54 proteins (≈19%) were exclusive. Saliva processing reduced identified proteins to 222 (≈80%) for the 0.22 μm group, 219 (≈79%) for the 0.45 μm group, and 201 (≈72%) for the pasteurized saliva, compared to non-processed saliva. The proteomic profile showed similar molecular functions and biological processes. The different saliva processing methods did not alter microbial adhesion (ANOVA, p > 0.05). Interestingly, pasteurized saliva reduced keratinocyte cell viability. Saliva processing methods tested reduced the proteomic profile diversity of saliva but maintained similar molecular functions and biological processes, not interfering with microbial adhesion and cell viability, except for pasteurization, which reduced cell viability.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Prosthodontics and Periodontology Piracicaba Dental School University of Campinas (UNICAMP), Sa̅o PauloDepartment of Restorative Dentistry Federal University of Paraná (UFPR), ParanáDepartment of Periodontology Dental Research Division Guarulhos University (UnG), Sa̅o PauloDepartment of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP), São PauloDepartment of Dental Materials and Prosthodontics School of Dentistry at Araçatuba São Paulo State University (UNESP), São PauloDepartment of Periodontics and Preventive Dentistry School of Dental Medicine University of PittsburghDental Science School (Faculdade de Ciências Odontológicas - FCO), Minas GeraisDepartment of Dental Materials and Prosthodontics School of Dentistry at Araraquara São Paulo State University (UNESP), São PauloDepartment of Dental Materials and Prosthodontics School of Dentistry at Araçatuba São Paulo State University (UNESP), São PauloUniversidade Estadual de Campinas (UNICAMP)Universidade Federal do Paraná (UFPR)Guarulhos University (UnG)Universidade Estadual Paulista (UNESP)University of PittsburghDental Science School (Faculdade de Ciências Odontológicas - FCO)Miranda, Luis Fernando B.Lima, Carolina V.Pagin, RafaelaCosta, Raphael C.Pereira, Marta Maria A. [UNESP]de Avila, Erica D. [UNESP]Bertolini, MartinnaRetamal-Valdes, BelénShibli, Jamil A.Feres, MagdaBarão, Valentim A. R.Souza, João Gabriel S.2023-07-29T13:42:19Z2023-07-29T13:42:19Z2023-03-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article857-870http://dx.doi.org/10.1021/acs.jproteome.2c00652Journal of Proteome Research, v. 22, n. 3, p. 857-870, 2023.1535-39071535-3893http://hdl.handle.net/11449/24837410.1021/acs.jproteome.2c006522-s2.0-85148299432Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Proteome Researchinfo:eu-repo/semantics/openAccess2023-07-29T13:42:20Zoai:repositorio.unesp.br:11449/248374Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:44:40.340849Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes |
title |
Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes |
spellingShingle |
Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes Miranda, Luis Fernando B. microbiology proteomic saliva sterilization study model |
title_short |
Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes |
title_full |
Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes |
title_fullStr |
Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes |
title_full_unstemmed |
Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes |
title_sort |
Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes |
author |
Miranda, Luis Fernando B. |
author_facet |
Miranda, Luis Fernando B. Lima, Carolina V. Pagin, Rafaela Costa, Raphael C. Pereira, Marta Maria A. [UNESP] de Avila, Erica D. [UNESP] Bertolini, Martinna Retamal-Valdes, Belén Shibli, Jamil A. Feres, Magda Barão, Valentim A. R. Souza, João Gabriel S. |
author_role |
author |
author2 |
Lima, Carolina V. Pagin, Rafaela Costa, Raphael C. Pereira, Marta Maria A. [UNESP] de Avila, Erica D. [UNESP] Bertolini, Martinna Retamal-Valdes, Belén Shibli, Jamil A. Feres, Magda Barão, Valentim A. R. Souza, João Gabriel S. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Federal do Paraná (UFPR) Guarulhos University (UnG) Universidade Estadual Paulista (UNESP) University of Pittsburgh Dental Science School (Faculdade de Ciências Odontológicas - FCO) |
dc.contributor.author.fl_str_mv |
Miranda, Luis Fernando B. Lima, Carolina V. Pagin, Rafaela Costa, Raphael C. Pereira, Marta Maria A. [UNESP] de Avila, Erica D. [UNESP] Bertolini, Martinna Retamal-Valdes, Belén Shibli, Jamil A. Feres, Magda Barão, Valentim A. R. Souza, João Gabriel S. |
dc.subject.por.fl_str_mv |
microbiology proteomic saliva sterilization study model |
topic |
microbiology proteomic saliva sterilization study model |
description |
The use of saliva as a protein source prior to microbiological and biological assays requires previous processing. However, the effect of these processing methods on the proteomic profile of saliva has not been tested. Stimulated human saliva was collected from eight healthy volunteers. Non-processed saliva was compared with 0.22 μm filtered, 0.45 μm filtered, and pasteurized saliva, by liquid chromatography-mass spectrometry. Data are available via ProteomeXchange with identifier PXD039248. The effect of processed saliva on microbial adhesion was tested using bacterial and fungus species and in biological cell behavior using HaCaT immortalized human keratinocytes. Two hundred and seventy-eight proteins were identified in non-processed saliva, of which 54 proteins (≈19%) were exclusive. Saliva processing reduced identified proteins to 222 (≈80%) for the 0.22 μm group, 219 (≈79%) for the 0.45 μm group, and 201 (≈72%) for the pasteurized saliva, compared to non-processed saliva. The proteomic profile showed similar molecular functions and biological processes. The different saliva processing methods did not alter microbial adhesion (ANOVA, p > 0.05). Interestingly, pasteurized saliva reduced keratinocyte cell viability. Saliva processing methods tested reduced the proteomic profile diversity of saliva but maintained similar molecular functions and biological processes, not interfering with microbial adhesion and cell viability, except for pasteurization, which reduced cell viability. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:42:19Z 2023-07-29T13:42:19Z 2023-03-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1021/acs.jproteome.2c00652 Journal of Proteome Research, v. 22, n. 3, p. 857-870, 2023. 1535-3907 1535-3893 http://hdl.handle.net/11449/248374 10.1021/acs.jproteome.2c00652 2-s2.0-85148299432 |
url |
http://dx.doi.org/10.1021/acs.jproteome.2c00652 http://hdl.handle.net/11449/248374 |
identifier_str_mv |
Journal of Proteome Research, v. 22, n. 3, p. 857-870, 2023. 1535-3907 1535-3893 10.1021/acs.jproteome.2c00652 2-s2.0-85148299432 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Proteome Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
857-870 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129112563253248 |