Pure and mixed biofilms formation of Listeria monocytogenes and Salmonella Typhimurium on polypropylene surfaces
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.lwt.2022.113469 http://hdl.handle.net/11449/239955 |
Resumo: | This study evaluated the capacity of Listeria monocytogenes and Salmonella Typhimurium to form pure and mixed biofilms on polypropylene surface and analyzed the expression of genes related to biofilm formation within 24 h and 120 h. The planktonic and sessile growth of L. monocytogenes and S. Typhimurium were related to incubation temperature. There was no statistical difference between the total population not only in pure L. monocytogenes and pure S. Typhimurium biofilms, but also in mixed biofilms at 12 °C and 37 °C. Pure and mixed biofilms were able to maintain viable cells for 3000 h at 10 °C with counts greater than when incubated for 120 h, with a greater population increase in mixed biofilms. Regarding the 24 h and 120 h biofilms of L. monocytogenes in both pure and mixed conditions at 12 °C and 37 °C, we were able to find expression of prfA, agrB, agrC and agrD genes. Concerning S. Typhimurium biofilms, we found expression of agfA, adrA and csgD genes in all situations evaluated, except for mixed S. Typhimurium biofilm at 12 °C for 24 h. This article documents the ability of L. monocytogenes and S. Typhimurium to form mixed biofilms and suggests that these two microorganisms may act synergistically. |
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Pure and mixed biofilms formation of Listeria monocytogenes and Salmonella Typhimurium on polypropylene surfacesBiofilmsFood industryGene expressionPersistenceThis study evaluated the capacity of Listeria monocytogenes and Salmonella Typhimurium to form pure and mixed biofilms on polypropylene surface and analyzed the expression of genes related to biofilm formation within 24 h and 120 h. The planktonic and sessile growth of L. monocytogenes and S. Typhimurium were related to incubation temperature. There was no statistical difference between the total population not only in pure L. monocytogenes and pure S. Typhimurium biofilms, but also in mixed biofilms at 12 °C and 37 °C. Pure and mixed biofilms were able to maintain viable cells for 3000 h at 10 °C with counts greater than when incubated for 120 h, with a greater population increase in mixed biofilms. Regarding the 24 h and 120 h biofilms of L. monocytogenes in both pure and mixed conditions at 12 °C and 37 °C, we were able to find expression of prfA, agrB, agrC and agrD genes. Concerning S. Typhimurium biofilms, we found expression of agfA, adrA and csgD genes in all situations evaluated, except for mixed S. Typhimurium biofilm at 12 °C for 24 h. This article documents the ability of L. monocytogenes and S. Typhimurium to form mixed biofilms and suggests that these two microorganisms may act synergistically.Universidade Federal do Paraná Setor Palotina Departamento de Ciências Veterinárias, Rua Pioneiro, 2153, Jardim Dallas, PRUniversidade Estadual Paulista (UNESP) Campus de Botucatu Faculdade de Medicina Veterinária e Zootecnia, Distrito de Rubião Jr, SN, São PauloUniversidade Federal de Viçosa Departamento de Veterinária, Avenida PH Rolfs, s/n, Campus Universitário, MGUniversidade Estadual Paulista (UNESP) Campus de Botucatu Faculdade de Medicina Veterinária e Zootecnia, Distrito de Rubião Jr, SN, São PauloUniversidade Federal do Paraná (UFPR)Universidade Estadual Paulista (UNESP)Universidade Federal de Viçosa (UFV)Tadielo, Leonardo Ereno [UNESP]Bellé, Thiago HenriqueSantos, Emanoelli Aparecida Rodrigues dos [UNESP]Schmiedt, Jhennifer ArrudaCerqueira-Cézar, Camila Koutsodontis [UNESP]Nero, Luis AugustoYamatogi, Ricardo SeitiPereira, Juliano Gonçalves [UNESP]Bersot, Luciano dos Santos2023-03-01T19:54:58Z2023-03-01T19:54:58Z2022-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.lwt.2022.113469LWT, v. 162.0023-6438http://hdl.handle.net/11449/23995510.1016/j.lwt.2022.1134692-s2.0-85129027806Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLWTinfo:eu-repo/semantics/openAccess2023-03-01T19:54:59Zoai:repositorio.unesp.br:11449/239955Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-05-23T13:19:52.807085Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Pure and mixed biofilms formation of Listeria monocytogenes and Salmonella Typhimurium on polypropylene surfaces |
title |
Pure and mixed biofilms formation of Listeria monocytogenes and Salmonella Typhimurium on polypropylene surfaces |
spellingShingle |
Pure and mixed biofilms formation of Listeria monocytogenes and Salmonella Typhimurium on polypropylene surfaces Tadielo, Leonardo Ereno [UNESP] Biofilms Food industry Gene expression Persistence |
title_short |
Pure and mixed biofilms formation of Listeria monocytogenes and Salmonella Typhimurium on polypropylene surfaces |
title_full |
Pure and mixed biofilms formation of Listeria monocytogenes and Salmonella Typhimurium on polypropylene surfaces |
title_fullStr |
Pure and mixed biofilms formation of Listeria monocytogenes and Salmonella Typhimurium on polypropylene surfaces |
title_full_unstemmed |
Pure and mixed biofilms formation of Listeria monocytogenes and Salmonella Typhimurium on polypropylene surfaces |
title_sort |
Pure and mixed biofilms formation of Listeria monocytogenes and Salmonella Typhimurium on polypropylene surfaces |
author |
Tadielo, Leonardo Ereno [UNESP] |
author_facet |
Tadielo, Leonardo Ereno [UNESP] Bellé, Thiago Henrique Santos, Emanoelli Aparecida Rodrigues dos [UNESP] Schmiedt, Jhennifer Arruda Cerqueira-Cézar, Camila Koutsodontis [UNESP] Nero, Luis Augusto Yamatogi, Ricardo Seiti Pereira, Juliano Gonçalves [UNESP] Bersot, Luciano dos Santos |
author_role |
author |
author2 |
Bellé, Thiago Henrique Santos, Emanoelli Aparecida Rodrigues dos [UNESP] Schmiedt, Jhennifer Arruda Cerqueira-Cézar, Camila Koutsodontis [UNESP] Nero, Luis Augusto Yamatogi, Ricardo Seiti Pereira, Juliano Gonçalves [UNESP] Bersot, Luciano dos Santos |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Paraná (UFPR) Universidade Estadual Paulista (UNESP) Universidade Federal de Viçosa (UFV) |
dc.contributor.author.fl_str_mv |
Tadielo, Leonardo Ereno [UNESP] Bellé, Thiago Henrique Santos, Emanoelli Aparecida Rodrigues dos [UNESP] Schmiedt, Jhennifer Arruda Cerqueira-Cézar, Camila Koutsodontis [UNESP] Nero, Luis Augusto Yamatogi, Ricardo Seiti Pereira, Juliano Gonçalves [UNESP] Bersot, Luciano dos Santos |
dc.subject.por.fl_str_mv |
Biofilms Food industry Gene expression Persistence |
topic |
Biofilms Food industry Gene expression Persistence |
description |
This study evaluated the capacity of Listeria monocytogenes and Salmonella Typhimurium to form pure and mixed biofilms on polypropylene surface and analyzed the expression of genes related to biofilm formation within 24 h and 120 h. The planktonic and sessile growth of L. monocytogenes and S. Typhimurium were related to incubation temperature. There was no statistical difference between the total population not only in pure L. monocytogenes and pure S. Typhimurium biofilms, but also in mixed biofilms at 12 °C and 37 °C. Pure and mixed biofilms were able to maintain viable cells for 3000 h at 10 °C with counts greater than when incubated for 120 h, with a greater population increase in mixed biofilms. Regarding the 24 h and 120 h biofilms of L. monocytogenes in both pure and mixed conditions at 12 °C and 37 °C, we were able to find expression of prfA, agrB, agrC and agrD genes. Concerning S. Typhimurium biofilms, we found expression of agfA, adrA and csgD genes in all situations evaluated, except for mixed S. Typhimurium biofilm at 12 °C for 24 h. This article documents the ability of L. monocytogenes and S. Typhimurium to form mixed biofilms and suggests that these two microorganisms may act synergistically. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-01 2023-03-01T19:54:58Z 2023-03-01T19:54:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.lwt.2022.113469 LWT, v. 162. 0023-6438 http://hdl.handle.net/11449/239955 10.1016/j.lwt.2022.113469 2-s2.0-85129027806 |
url |
http://dx.doi.org/10.1016/j.lwt.2022.113469 http://hdl.handle.net/11449/239955 |
identifier_str_mv |
LWT, v. 162. 0023-6438 10.1016/j.lwt.2022.113469 2-s2.0-85129027806 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
LWT |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045606702186496 |