A look into Phaffia rhodozyma biorefinery: From the recovery and fractionation of carotenoids, lipids and proteins to the sustainable manufacturing of biologically active bioplastics

• Autor(a) principal: Mussagy, Cassamo U.
• Data de Publicação: 2022
• Outros Autores: Remonatto, Daniela [UNESP], Picheli, Flavio P. [UNESP], Paula, Ariela V. [UNESP], Herculano, Rondinelli D. [UNESP], Santos-Ebinuma, Valéria C. [UNESP], Farias, Renan L., Onishi, Bruno S. D. [UNESP], Ribeiro, Sidney J. L. [UNESP], Pereira, Jorge F. B. [UNESP], Pessoa, Adalberto
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.biortech.2022.127785; http://hdl.handle.net/11449/240657
• topic: Astaxanthin; Bioplastics; Biorefinery; Biosolvents; Phaffia rhodozyma; β-carotene

Carotenoids over-producing yeast has become a focus of interest of the biorefineries, in which the integration of the bioproduction with the following downstream processing units for the recovery and purification of carotenoids and other value-added byproducts is crucial to improve the sustainability and profitability of the overall bioprocess. Aiming the future implementation of Phaffia rhodozyma-based biorefineries, in this work, an integrative process for fractionation of intracellular compounds from P. rhodozyma biomass using non-hazardous bio-based solvents was developed. After one-extraction step, the total amount of astaxanthin, β-carotene, lipids and proteins recovered was 63.11 µg/gDCW, 42.81 µg/gDCW, 53.75 mg/gDCW and 10.93 mg/g, respectively. The implementation of sequential back-extraction processes and integration with saponification and precipitation operations allowed the efficient fractionation and recovery (% w/w) of astaxanthin (∼72.5 %), β-carotene ∼90.17 %), proteins (21.04 %) and lipids (23.72 %). After fractionation, the manufacture of carotenoids-based products was demonstrated, through the mixture of carotenoids-rich extracts with bacterial cellulose to obtain biologically active bioplastics.