Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.eti.2023.103029 http://hdl.handle.net/11449/246759 |
Resumo: | Microbial-based pigments have attracted the interest of the cleaning and cosmeceutical industries as greener and more effective alternatives to the synthetic antioxidants and antimicrobials used in commercially colored soaps. Astaxanthin and β-carotene are natural carotenoids with strong antioxidant activity produced by yeast Phaffia rhodozyma (P. rhodozyma), but its use in industrial-scale processes is still very scarce. The optimization of downstream processing operations for the recovery of carotenoids from P. rhodozyma using environmentally and energetically favorable processes has not yet been fully achieved. In the present work, we propose a simple and sustainable one-pot process using biocompatible and renewable solvents (ethanolic carboxylic acids mixtures) for the recovery of astaxanthin and β-carotene from P. rhodozyma biomass and direct formulation of bioactive soaps, thus minimizing the processing units. For this purpose, carotenoids-enriched ethanolic carboxylic acids solutions were directly (“one-pot”) used in the following saponification process for the formulation of bioactive soaps without the need for additional purification units. The recovery aptitude of ethanolic carboxylic acids solutions and the corresponding solvation mechanisms of different solvent mixtures for astaxanthin and β-carotene were screened using the COSMO-SAC model. Operating parameters (e.g., solvent ratio, time extraction, temperature, and re-extraction cycles) were optimized for the solvent mixture (ethanol:lauric acid) that exhibited the highest recovery capacity for both carotenoids. The incorporation of microbial carotenoids in formulated soaps decreases the moisture content (from 10.04 to 6.85%), the pH (from 8.83 to 8.68), and the surface hardness (from 0.68 to 0.60 mm) and increase the foam height (from 5.83 to 7.07 cm). The biological activity of carotenoids-rich soaps was confirmed by antioxidant tests (60.53%). This work demonstrated that mixing natural yeast-based carotenoids with biosolvents is a simple, effective and sustainable solution for the formulation of functional and colored soaps. |
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Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulationAstaxanthinCarboxylic acidsNatural antioxidantsPhaffia rhodozymaSoap formulationβ-caroteneMicrobial-based pigments have attracted the interest of the cleaning and cosmeceutical industries as greener and more effective alternatives to the synthetic antioxidants and antimicrobials used in commercially colored soaps. Astaxanthin and β-carotene are natural carotenoids with strong antioxidant activity produced by yeast Phaffia rhodozyma (P. rhodozyma), but its use in industrial-scale processes is still very scarce. The optimization of downstream processing operations for the recovery of carotenoids from P. rhodozyma using environmentally and energetically favorable processes has not yet been fully achieved. In the present work, we propose a simple and sustainable one-pot process using biocompatible and renewable solvents (ethanolic carboxylic acids mixtures) for the recovery of astaxanthin and β-carotene from P. rhodozyma biomass and direct formulation of bioactive soaps, thus minimizing the processing units. For this purpose, carotenoids-enriched ethanolic carboxylic acids solutions were directly (“one-pot”) used in the following saponification process for the formulation of bioactive soaps without the need for additional purification units. The recovery aptitude of ethanolic carboxylic acids solutions and the corresponding solvation mechanisms of different solvent mixtures for astaxanthin and β-carotene were screened using the COSMO-SAC model. Operating parameters (e.g., solvent ratio, time extraction, temperature, and re-extraction cycles) were optimized for the solvent mixture (ethanol:lauric acid) that exhibited the highest recovery capacity for both carotenoids. The incorporation of microbial carotenoids in formulated soaps decreases the moisture content (from 10.04 to 6.85%), the pH (from 8.83 to 8.68), and the surface hardness (from 0.68 to 0.60 mm) and increase the foam height (from 5.83 to 7.07 cm). The biological activity of carotenoids-rich soaps was confirmed by antioxidant tests (60.53%). This work demonstrated that mixing natural yeast-based carotenoids with biosolvents is a simple, effective and sustainable solution for the formulation of functional and colored soaps.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Escuela de Agronomía Facultad de Ciencias Agronómicas y de los Alimentos Pontificia Universidad Católica de ValparaísoDepartment of Chemical Engineering Federal University of ParanáDepartment of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP) AraraquaraUniv Coimbra CIEPQPF Department of Chemical Engineering, Rua Sílvio Lima, Pólo II – Pinhal de MarrocosDepartment of Pharmaceutical-Biochemical Technology School of Pharmaceutical Sciences University of São PauloDepartment of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical Sciences São Paulo State University (UNESP) AraraquaraCNPq: 312463/2021-9Pontificia Universidad Católica de ValparaísoFederal University of ParanáUniversidade Estadual Paulista (UNESP)CIEPQPFUniversidade de São Paulo (USP)Mussagy, Cassamo U.Farias, Fabiane O.Santos-Ebinuma, Valeria C. [UNESP]Pereira, Jorge F.B.Pessoa, Adalberto2023-07-29T12:49:47Z2023-07-29T12:49:47Z2023-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.eti.2023.103029Environmental Technology and Innovation, v. 29.2352-1864http://hdl.handle.net/11449/24675910.1016/j.eti.2023.1030292-s2.0-85147327652Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Technology and Innovationinfo:eu-repo/semantics/openAccess2023-07-29T12:49:47Zoai:repositorio.unesp.br:11449/246759Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:47:57.047466Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation |
title |
Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation |
spellingShingle |
Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation Mussagy, Cassamo U. Astaxanthin Carboxylic acids Natural antioxidants Phaffia rhodozyma Soap formulation β-carotene |
title_short |
Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation |
title_full |
Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation |
title_fullStr |
Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation |
title_full_unstemmed |
Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation |
title_sort |
Sustainable one-pot platform for the green recovery of carotenoids from Phaffia rhodozyma yeast and their use as natural additives in soap formulation |
author |
Mussagy, Cassamo U. |
author_facet |
Mussagy, Cassamo U. Farias, Fabiane O. Santos-Ebinuma, Valeria C. [UNESP] Pereira, Jorge F.B. Pessoa, Adalberto |
author_role |
author |
author2 |
Farias, Fabiane O. Santos-Ebinuma, Valeria C. [UNESP] Pereira, Jorge F.B. Pessoa, Adalberto |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Pontificia Universidad Católica de Valparaíso Federal University of Paraná Universidade Estadual Paulista (UNESP) CIEPQPF Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Mussagy, Cassamo U. Farias, Fabiane O. Santos-Ebinuma, Valeria C. [UNESP] Pereira, Jorge F.B. Pessoa, Adalberto |
dc.subject.por.fl_str_mv |
Astaxanthin Carboxylic acids Natural antioxidants Phaffia rhodozyma Soap formulation β-carotene |
topic |
Astaxanthin Carboxylic acids Natural antioxidants Phaffia rhodozyma Soap formulation β-carotene |
description |
Microbial-based pigments have attracted the interest of the cleaning and cosmeceutical industries as greener and more effective alternatives to the synthetic antioxidants and antimicrobials used in commercially colored soaps. Astaxanthin and β-carotene are natural carotenoids with strong antioxidant activity produced by yeast Phaffia rhodozyma (P. rhodozyma), but its use in industrial-scale processes is still very scarce. The optimization of downstream processing operations for the recovery of carotenoids from P. rhodozyma using environmentally and energetically favorable processes has not yet been fully achieved. In the present work, we propose a simple and sustainable one-pot process using biocompatible and renewable solvents (ethanolic carboxylic acids mixtures) for the recovery of astaxanthin and β-carotene from P. rhodozyma biomass and direct formulation of bioactive soaps, thus minimizing the processing units. For this purpose, carotenoids-enriched ethanolic carboxylic acids solutions were directly (“one-pot”) used in the following saponification process for the formulation of bioactive soaps without the need for additional purification units. The recovery aptitude of ethanolic carboxylic acids solutions and the corresponding solvation mechanisms of different solvent mixtures for astaxanthin and β-carotene were screened using the COSMO-SAC model. Operating parameters (e.g., solvent ratio, time extraction, temperature, and re-extraction cycles) were optimized for the solvent mixture (ethanol:lauric acid) that exhibited the highest recovery capacity for both carotenoids. The incorporation of microbial carotenoids in formulated soaps decreases the moisture content (from 10.04 to 6.85%), the pH (from 8.83 to 8.68), and the surface hardness (from 0.68 to 0.60 mm) and increase the foam height (from 5.83 to 7.07 cm). The biological activity of carotenoids-rich soaps was confirmed by antioxidant tests (60.53%). This work demonstrated that mixing natural yeast-based carotenoids with biosolvents is a simple, effective and sustainable solution for the formulation of functional and colored soaps. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:49:47Z 2023-07-29T12:49:47Z 2023-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.eti.2023.103029 Environmental Technology and Innovation, v. 29. 2352-1864 http://hdl.handle.net/11449/246759 10.1016/j.eti.2023.103029 2-s2.0-85147327652 |
url |
http://dx.doi.org/10.1016/j.eti.2023.103029 http://hdl.handle.net/11449/246759 |
identifier_str_mv |
Environmental Technology and Innovation, v. 29. 2352-1864 10.1016/j.eti.2023.103029 2-s2.0-85147327652 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Technology and Innovation |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128564207288320 |