Microdissection and Chromosome Painting

• Autor(a) principal: Vicari, M. R.
• Data de Publicação: 2015
• Outros Autores: Pansonato-Alves, J. C. [UNESP], Foresti, F. [UNESP]
• Tipo de documento: Capítulo de livro
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1201/b18534-21; http://hdl.handle.net/11449/247569

The principle of chromosome microdissection technique is based on the identification of either a chromosome, chromosome arm or chromosome band for the subsequent removal of such target with the aid of a micromanipulator attached to an inverted microscope. Once the target is defined, whole chromosomes, or chromosomal regions, are directly isolated under an inverted microscope linked to a micromanipulator. Following dissection, chromosomal fragments pass through a step of amplification, using adapted methods of whole genome amplification (WGA) in order to increase the limited amount of DNA (Telenius et al. 1992; Zhang et al. 1992) and reduce the starting copies material (Fig. 1).