Microdissection and Chromosome Painting
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Capítulo de livro |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1201/b18534-21 http://hdl.handle.net/11449/247569 |
Resumo: | The principle of chromosome microdissection technique is based on the identification of either a chromosome, chromosome arm or chromosome band for the subsequent removal of such target with the aid of a micromanipulator attached to an inverted microscope. Once the target is defined, whole chromosomes, or chromosomal regions, are directly isolated under an inverted microscope linked to a micromanipulator. Following dissection, chromosomal fragments pass through a step of amplification, using adapted methods of whole genome amplification (WGA) in order to increase the limited amount of DNA (Telenius et al. 1992; Zhang et al. 1992) and reduce the starting copies material (Fig. 1). |
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Microdissection and Chromosome PaintingThe principle of chromosome microdissection technique is based on the identification of either a chromosome, chromosome arm or chromosome band for the subsequent removal of such target with the aid of a micromanipulator attached to an inverted microscope. Once the target is defined, whole chromosomes, or chromosomal regions, are directly isolated under an inverted microscope linked to a micromanipulator. Following dissection, chromosomal fragments pass through a step of amplification, using adapted methods of whole genome amplification (WGA) in order to increase the limited amount of DNA (Telenius et al. 1992; Zhang et al. 1992) and reduce the starting copies material (Fig. 1).Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Departamento de Biologia Estrutural Laboratório de Citogenética e Evolução Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, ParanáDepartamento de Morfologia Universidade Estadual Paulista, Distrito de Rubião Júnior, s/n, São PauloDepartamento de Morfologia Universidade Estadual Paulista, Distrito de Rubião Júnior, s/n, São PauloUniversidade Estadual de Ponta Grossa (UEPG)Universidade Estadual Paulista (UNESP)Vicari, M. R.Pansonato-Alves, J. C. [UNESP]Foresti, F. [UNESP]2023-07-29T13:19:41Z2023-07-29T13:19:41Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookPart144-165http://dx.doi.org/10.1201/b18534-21Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans, p. 144-165.http://hdl.handle.net/11449/24756910.1201/b18534-212-s2.0-85075122135Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyansinfo:eu-repo/semantics/openAccess2023-07-29T13:19:41Zoai:repositorio.unesp.br:11449/247569Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:23:51.727551Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Microdissection and Chromosome Painting |
title |
Microdissection and Chromosome Painting |
spellingShingle |
Microdissection and Chromosome Painting Vicari, M. R. |
title_short |
Microdissection and Chromosome Painting |
title_full |
Microdissection and Chromosome Painting |
title_fullStr |
Microdissection and Chromosome Painting |
title_full_unstemmed |
Microdissection and Chromosome Painting |
title_sort |
Microdissection and Chromosome Painting |
author |
Vicari, M. R. |
author_facet |
Vicari, M. R. Pansonato-Alves, J. C. [UNESP] Foresti, F. [UNESP] |
author_role |
author |
author2 |
Pansonato-Alves, J. C. [UNESP] Foresti, F. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Ponta Grossa (UEPG) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Vicari, M. R. Pansonato-Alves, J. C. [UNESP] Foresti, F. [UNESP] |
description |
The principle of chromosome microdissection technique is based on the identification of either a chromosome, chromosome arm or chromosome band for the subsequent removal of such target with the aid of a micromanipulator attached to an inverted microscope. Once the target is defined, whole chromosomes, or chromosomal regions, are directly isolated under an inverted microscope linked to a micromanipulator. Following dissection, chromosomal fragments pass through a step of amplification, using adapted methods of whole genome amplification (WGA) in order to increase the limited amount of DNA (Telenius et al. 1992; Zhang et al. 1992) and reduce the starting copies material (Fig. 1). |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2023-07-29T13:19:41Z 2023-07-29T13:19:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/bookPart |
format |
bookPart |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1201/b18534-21 Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans, p. 144-165. http://hdl.handle.net/11449/247569 10.1201/b18534-21 2-s2.0-85075122135 |
url |
http://dx.doi.org/10.1201/b18534-21 http://hdl.handle.net/11449/247569 |
identifier_str_mv |
Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans, p. 144-165. 10.1201/b18534-21 2-s2.0-85075122135 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fish Cytogenetic Techniques: Ray-Fin Fishes and Chondrichthyans |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
144-165 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129516560711680 |