Gamma-oryzanol as a potential modulator of oxidative stress and inflammation via PPAR-y in adipose tissue: a hypothetical therapeutic for cytokine storm in COVID-19?

• Autor(a) principal: Francisqueti-Ferron, Fabiane Valentini [UNESP]
• Data de Publicação: 2021
• Outros Autores: Garcia, Jéssica Leite [UNESP], Ferron, Artur Junio Togneri [UNESP], Nakandakare-Maia, Erika Tiemi [UNESP], Gregolin, Cristina Schmitt [UNESP], Silva, Janaina Paixão das Chagas [UNESP], dos Santos, Klinsmann Carolo [UNESP], Lo, Ângelo Thompson Colombo [UNESP], Siqueira, Juliana Silva [UNESP], de Mattei, Letícia [UNESP], de Paula, Bruno Henrique [UNESP], Sarzi, Felipe, Silva, Carol Cristina Vágula de Almeida [UNESP], Moreto, Fernando [UNESP], Costa, Mariane Róvero [UNESP], Ferreira, Ana Lucia A. [UNESP], Minatel, Igor Otávio [UNESP], Corrêa, Camila Renata [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.mce.2020.111095; http://hdl.handle.net/11449/205568
• topic: COVID-19; Gamma-oryzanol; Obesity; PPAR-γ

The literature has reported a higher prevalence of negative clinical outcomes due to Coronavirus disease 19 (COVID-19) in obese individuals. This can be explained by the cytokine storm, result from the cytokine production from both obesity and viral infection. Gamma-oryzanol (γOz) is a compound with anti-inflammatory and antioxidant activities. However, little is known about the γOz action as a possible agonist of peroxisome proliferator-activated receptor gamma (PPAR-γ). The aim of this study was to test the hypothesis that γOz attenuates the cytokine storm by stimulating PPAR-γ in the adipose tissue. Methods: Male Wistar rats were randomly divided into three experimental groups and fed ad libitum for 30 weeks with control diet (C, n = 6), high sugar-fat diet (HSF, n = 6) or high sugar-fat diet + γOz (HSF + γOz, n = 6). HSF groups also received water + sucrose (25%). The γOz dose was 0.5% in the chow. Evaluation in animals included caloric intake, body weight, adiposity index, plasma triglycerides, and HOMA-IR. In adipose tissue was evaluated: PPAR-γ gene and protein expression, inflammatory and oxidative stress parameters, and histological analysis. Results: Adipose tissue dysfunction was observed in HSF group, which presented remarkable PPAR-γ underexpression and increased levels of cytokines, other inflammatory markers and oxidative stress. The γOz treatment prevented adipose tissue dysfunction and promoted PPAR-γ overexpression. Conclusion: Natural compounds as γOz can be considered a coadjutant therapy to prevent the cytokine storm in COVID-19 patients with obesity conditions.