Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3897/CompCytogen.v14i2.49513 http://hdl.handle.net/11449/206559 |
Resumo: | Astyanax Baird et Girard, 1854, is one of the largest genera in the family Characidae and comprises 177 valid species. This genus has been the focus of cytogenetic studies primarily owing to the presence of B chromosomes and high karyotypic diversity among different populations. The intense genetic variability in Astyanax is one of the factors responsible for the occurrence of species complexes, which are groups (1) with certain difficulties in establishing common genetic pools or (2) belonging to different cryptic species. To evaluate cytogenetic marker inheritance and the possibility of the identification of these hybrids, this study aimed to describe cytogenetic hybrids from three strains of species of the genera Astyanax and Hyphessobrycon Eigenmann, 1908. A. lacustris Lutken, 1875, A. schubarti Britski, 1964, A. fasciatus Cuvier, 1819, and H. anisitsi Eigenmann, 1907 were used to generate three hybrid lineages. The diploid number, heterochromatin sites, and ribosomal genes (18S and 5S rDNA) of the parental strains and the hybrids were analyzed. The results indicated that the three hybrid lineages had cytogenetic markers of both parents, presenting Mendelian inheritance. However, differences in distribution of heterochromatic blocks were observed between the hybrids and the parent strains. Our results allowed the identification of the hybrid strains based on the cytogenetic markers applied, reinforcing the efficiency of cytogenetic markers as tools for identification and indicating that such events may increase the karyotypic diversity in the genera Astyanax and Hyphessobrycon. |
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Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907B chromosomesChromosome polymorphismNeotropical fishesRepetitive DNAsSpecies complexAstyanax Baird et Girard, 1854, is one of the largest genera in the family Characidae and comprises 177 valid species. This genus has been the focus of cytogenetic studies primarily owing to the presence of B chromosomes and high karyotypic diversity among different populations. The intense genetic variability in Astyanax is one of the factors responsible for the occurrence of species complexes, which are groups (1) with certain difficulties in establishing common genetic pools or (2) belonging to different cryptic species. To evaluate cytogenetic marker inheritance and the possibility of the identification of these hybrids, this study aimed to describe cytogenetic hybrids from three strains of species of the genera Astyanax and Hyphessobrycon Eigenmann, 1908. A. lacustris Lutken, 1875, A. schubarti Britski, 1964, A. fasciatus Cuvier, 1819, and H. anisitsi Eigenmann, 1907 were used to generate three hybrid lineages. The diploid number, heterochromatin sites, and ribosomal genes (18S and 5S rDNA) of the parental strains and the hybrids were analyzed. The results indicated that the three hybrid lineages had cytogenetic markers of both parents, presenting Mendelian inheritance. However, differences in distribution of heterochromatic blocks were observed between the hybrids and the parent strains. Our results allowed the identification of the hybrid strains based on the cytogenetic markers applied, reinforcing the efficiency of cytogenetic markers as tools for identification and indicating that such events may increase the karyotypic diversity in the genera Astyanax and Hyphessobrycon.Universidade Estadual Paulista (UNESP) Júlio de Mesquita Filho Faculdade de Ciências Edmundo Carrijo Coube AvenueCentro nacional de Pesquisa e Conservação da Biota Aquatica Continental (CEPTA-ICMBIO) Prefeito Euberto Nemésio Pereira Godói HighwayUniversidade Estadual de Ponta Grossa Setor de Ciências Biológicas e da Saúde, Santos Andrade SquareUniversidade Estadual Paulista (UNESP) Júlio de Mesquita Filho Centro de Aquicultura da UNESP, Prof. Paulo Donato Castelane Acess wayUniversidade Estadual Paulista (UNESP) Júlio de Mesquita Filho Instituto de Biociências, Prof. Montenegro AvenueUniversidade Estadual Paulista (UNESP) Júlio de Mesquita Filho Faculdade de Ciências Edmundo Carrijo Coube AvenueUniversidade Estadual Paulista (UNESP) Júlio de Mesquita Filho Centro de Aquicultura da UNESP, Prof. Paulo Donato Castelane Acess wayUniversidade Estadual Paulista (UNESP) Júlio de Mesquita Filho Instituto de Biociências, Prof. Montenegro AvenueUniversidade Estadual Paulista (Unesp)Prefeito Euberto Nemésio Pereira Godói HighwayUniversidade Estadual de Ponta Grossa (UEPG)Goes, Caio Augusto Gomes [UNESP]Daniel, Sandro Natal [UNESP]Piva, Lucas HenriqueYasui, George ShiguekiArtoni, Roberto FerreiraHashimoto, Diogo Teruo [UNESP]Foresti, Fausto [UNESP]Porto-Foresti, Fábio [UNESP]2021-06-25T10:34:19Z2021-06-25T10:34:19Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article231-242http://dx.doi.org/10.3897/CompCytogen.v14i2.49513Comparative Cytogenetics, v. 14, n. 2, p. 231-242, 2020.1993-078X1993-0771http://hdl.handle.net/11449/20655910.3897/CompCytogen.v14i2.495132-s2.0-85091496725Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Cytogeneticsinfo:eu-repo/semantics/openAccess2024-04-09T15:29:36Zoai:repositorio.unesp.br:11449/206559Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:29:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 |
title |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 |
spellingShingle |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 Goes, Caio Augusto Gomes [UNESP] B chromosomes Chromosome polymorphism Neotropical fishes Repetitive DNAs Species complex |
title_short |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 |
title_full |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 |
title_fullStr |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 |
title_full_unstemmed |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 |
title_sort |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 |
author |
Goes, Caio Augusto Gomes [UNESP] |
author_facet |
Goes, Caio Augusto Gomes [UNESP] Daniel, Sandro Natal [UNESP] Piva, Lucas Henrique Yasui, George Shigueki Artoni, Roberto Ferreira Hashimoto, Diogo Teruo [UNESP] Foresti, Fausto [UNESP] Porto-Foresti, Fábio [UNESP] |
author_role |
author |
author2 |
Daniel, Sandro Natal [UNESP] Piva, Lucas Henrique Yasui, George Shigueki Artoni, Roberto Ferreira Hashimoto, Diogo Teruo [UNESP] Foresti, Fausto [UNESP] Porto-Foresti, Fábio [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Prefeito Euberto Nemésio Pereira Godói Highway Universidade Estadual de Ponta Grossa (UEPG) |
dc.contributor.author.fl_str_mv |
Goes, Caio Augusto Gomes [UNESP] Daniel, Sandro Natal [UNESP] Piva, Lucas Henrique Yasui, George Shigueki Artoni, Roberto Ferreira Hashimoto, Diogo Teruo [UNESP] Foresti, Fausto [UNESP] Porto-Foresti, Fábio [UNESP] |
dc.subject.por.fl_str_mv |
B chromosomes Chromosome polymorphism Neotropical fishes Repetitive DNAs Species complex |
topic |
B chromosomes Chromosome polymorphism Neotropical fishes Repetitive DNAs Species complex |
description |
Astyanax Baird et Girard, 1854, is one of the largest genera in the family Characidae and comprises 177 valid species. This genus has been the focus of cytogenetic studies primarily owing to the presence of B chromosomes and high karyotypic diversity among different populations. The intense genetic variability in Astyanax is one of the factors responsible for the occurrence of species complexes, which are groups (1) with certain difficulties in establishing common genetic pools or (2) belonging to different cryptic species. To evaluate cytogenetic marker inheritance and the possibility of the identification of these hybrids, this study aimed to describe cytogenetic hybrids from three strains of species of the genera Astyanax and Hyphessobrycon Eigenmann, 1908. A. lacustris Lutken, 1875, A. schubarti Britski, 1964, A. fasciatus Cuvier, 1819, and H. anisitsi Eigenmann, 1907 were used to generate three hybrid lineages. The diploid number, heterochromatin sites, and ribosomal genes (18S and 5S rDNA) of the parental strains and the hybrids were analyzed. The results indicated that the three hybrid lineages had cytogenetic markers of both parents, presenting Mendelian inheritance. However, differences in distribution of heterochromatic blocks were observed between the hybrids and the parent strains. Our results allowed the identification of the hybrid strains based on the cytogenetic markers applied, reinforcing the efficiency of cytogenetic markers as tools for identification and indicating that such events may increase the karyotypic diversity in the genera Astyanax and Hyphessobrycon. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 2021-06-25T10:34:19Z 2021-06-25T10:34:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3897/CompCytogen.v14i2.49513 Comparative Cytogenetics, v. 14, n. 2, p. 231-242, 2020. 1993-078X 1993-0771 http://hdl.handle.net/11449/206559 10.3897/CompCytogen.v14i2.49513 2-s2.0-85091496725 |
url |
http://dx.doi.org/10.3897/CompCytogen.v14i2.49513 http://hdl.handle.net/11449/206559 |
identifier_str_mv |
Comparative Cytogenetics, v. 14, n. 2, p. 231-242, 2020. 1993-078X 1993-0771 10.3897/CompCytogen.v14i2.49513 2-s2.0-85091496725 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Cytogenetics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
231-242 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046246917603328 |