Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3897/CompCytogen.v14i2.49513 http://hdl.handle.net/11449/196965 |
Resumo: | Astyanax Baird et Girard, 1854, is one of the largest genera in the family Characidac and comprises 177 valid species. This genus has been the focus of cytogenetic studies primarily owing to the presence of B chromosomes and high karyotypic diversity among different populations. The intense genetic variability in Astyanax is one of the factors responsible for the occurrence of species complexes, which are groups (1) with certain difficulties in establishing common genetic pools or (2) belonging to different cryptic species. To evaluate cytogenetic marker inheritance and the possibility of the identification of these hybrids, this study aimed to describe cytogenetic hybrids from three strains of species of the genera Astyanax and Hyphessobrycon Eigenmann, 1908. A. lacustris Lutken, 1875, A. schubarti Britski, 1964, A. fasciatus Cuvier, 1819, and H. anisitsi Eigenmann, 1907 were used to generate three hybrid lineages. The diploid number, heterochromatin sites, and ribosomal genes (18S and 5S rDNA) of the parental strains and the hybrids were analyzed. The results indicated that the three hybrid lineages had cytogenetic markers of both parents, presenting Mendelian inheritance. However, differences in distribution of heterochromatic blocks were observed between the hybrids and the parent strains. Our results allowed the identification of the hybrid strains based on the cytogenetic markers applied, reinforcing the efficiency of cytogenetic markers as tools for identification and indicating that such events may increase the karyotypic diversity in the genera Astyanax and Hyphessobrycon. |
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Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907neotropical fishesB chromosomeschromosome polymorphismrepetitive DNAsspecies complexAstyanax Baird et Girard, 1854, is one of the largest genera in the family Characidac and comprises 177 valid species. This genus has been the focus of cytogenetic studies primarily owing to the presence of B chromosomes and high karyotypic diversity among different populations. The intense genetic variability in Astyanax is one of the factors responsible for the occurrence of species complexes, which are groups (1) with certain difficulties in establishing common genetic pools or (2) belonging to different cryptic species. To evaluate cytogenetic marker inheritance and the possibility of the identification of these hybrids, this study aimed to describe cytogenetic hybrids from three strains of species of the genera Astyanax and Hyphessobrycon Eigenmann, 1908. A. lacustris Lutken, 1875, A. schubarti Britski, 1964, A. fasciatus Cuvier, 1819, and H. anisitsi Eigenmann, 1907 were used to generate three hybrid lineages. The diploid number, heterochromatin sites, and ribosomal genes (18S and 5S rDNA) of the parental strains and the hybrids were analyzed. The results indicated that the three hybrid lineages had cytogenetic markers of both parents, presenting Mendelian inheritance. However, differences in distribution of heterochromatic blocks were observed between the hybrids and the parent strains. Our results allowed the identification of the hybrid strains based on the cytogenetic markers applied, reinforcing the efficiency of cytogenetic markers as tools for identification and indicating that such events may increase the karyotypic diversity in the genera Astyanax and Hyphessobrycon.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista UNESP Julio de Mesquita Fi, Fac Ciencias, Edmundo Carrijo Coube Ave, Bauru, SP, BrazilCtr Nacl Pesquisa & Conservacao Biota Aquat Conti, Prefeito Uberto Nemesio Pereira Godoi Highways, Pirassununga, SP, BrazilUniv Estadual Ponta Grossa, Setor Ciencias Biol & Saude, Santos Andrade Sq, Ponta Grossa, Parana, BrazilUniv Estadual Paulista UNESP Julio de Mesquita Fi, Ctr Aquicultura UNESP, Prof Paulo Donato Castelane Acess Way, Jaboticabal, SP, BrazilUniv Estadual Paulista UNESP Julio de Mesquita Fi, Inst Biociencias, Prof Montenegro Ave, Botucatu, SP, BrazilUniv Estadual Paulista UNESP Julio de Mesquita Fi, Fac Ciencias, Edmundo Carrijo Coube Ave, Bauru, SP, BrazilUniv Estadual Paulista UNESP Julio de Mesquita Fi, Ctr Aquicultura UNESP, Prof Paulo Donato Castelane Acess Way, Jaboticabal, SP, BrazilUniv Estadual Paulista UNESP Julio de Mesquita Fi, Inst Biociencias, Prof Montenegro Ave, Botucatu, SP, BrazilFAPESP: 2015/12902-4Pensoft PublishersUniversidade Estadual Paulista (Unesp)Ctr Nacl Pesquisa & Conservacao Biota Aquat ContiUniversidade Estadual de Ponta Grossa (UEPG)Gomes Goes, Caio Augusto [UNESP]Daniel, Sandro Natal [UNESP]Piva, Lucas HenriqueYasui, George ShiguekiArtoni, Roberto FerreiraHashimoto, Diogo Teruo [UNESP]Foresti, Fausto [UNESP]Porto-Foresti, Fabio [UNESP]2020-12-10T20:01:57Z2020-12-10T20:01:57Z2020-05-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article231-242http://dx.doi.org/10.3897/CompCytogen.v14i2.49513Comparative Cytogenetics. Sofia: Pensoft Publishers, v. 14, n. 2, p. 231-242, 2020.1993-0771http://hdl.handle.net/11449/19696510.3897/CompCytogen.v14i2.49513WOS:000539067300001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Cytogeneticsinfo:eu-repo/semantics/openAccess2024-04-09T15:29:36Zoai:repositorio.unesp.br:11449/196965Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:29:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 |
title |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 |
spellingShingle |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 Gomes Goes, Caio Augusto [UNESP] neotropical fishes B chromosomes chromosome polymorphism repetitive DNAs species complex |
title_short |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 |
title_full |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 |
title_fullStr |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 |
title_full_unstemmed |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 |
title_sort |
Cytogenetic markers as a tool for characterization of hybrids of Astyanax Baird & Girard, 1854 and Hyphessobrycon Eigenmann, 1907 |
author |
Gomes Goes, Caio Augusto [UNESP] |
author_facet |
Gomes Goes, Caio Augusto [UNESP] Daniel, Sandro Natal [UNESP] Piva, Lucas Henrique Yasui, George Shigueki Artoni, Roberto Ferreira Hashimoto, Diogo Teruo [UNESP] Foresti, Fausto [UNESP] Porto-Foresti, Fabio [UNESP] |
author_role |
author |
author2 |
Daniel, Sandro Natal [UNESP] Piva, Lucas Henrique Yasui, George Shigueki Artoni, Roberto Ferreira Hashimoto, Diogo Teruo [UNESP] Foresti, Fausto [UNESP] Porto-Foresti, Fabio [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Ctr Nacl Pesquisa & Conservacao Biota Aquat Conti Universidade Estadual de Ponta Grossa (UEPG) |
dc.contributor.author.fl_str_mv |
Gomes Goes, Caio Augusto [UNESP] Daniel, Sandro Natal [UNESP] Piva, Lucas Henrique Yasui, George Shigueki Artoni, Roberto Ferreira Hashimoto, Diogo Teruo [UNESP] Foresti, Fausto [UNESP] Porto-Foresti, Fabio [UNESP] |
dc.subject.por.fl_str_mv |
neotropical fishes B chromosomes chromosome polymorphism repetitive DNAs species complex |
topic |
neotropical fishes B chromosomes chromosome polymorphism repetitive DNAs species complex |
description |
Astyanax Baird et Girard, 1854, is one of the largest genera in the family Characidac and comprises 177 valid species. This genus has been the focus of cytogenetic studies primarily owing to the presence of B chromosomes and high karyotypic diversity among different populations. The intense genetic variability in Astyanax is one of the factors responsible for the occurrence of species complexes, which are groups (1) with certain difficulties in establishing common genetic pools or (2) belonging to different cryptic species. To evaluate cytogenetic marker inheritance and the possibility of the identification of these hybrids, this study aimed to describe cytogenetic hybrids from three strains of species of the genera Astyanax and Hyphessobrycon Eigenmann, 1908. A. lacustris Lutken, 1875, A. schubarti Britski, 1964, A. fasciatus Cuvier, 1819, and H. anisitsi Eigenmann, 1907 were used to generate three hybrid lineages. The diploid number, heterochromatin sites, and ribosomal genes (18S and 5S rDNA) of the parental strains and the hybrids were analyzed. The results indicated that the three hybrid lineages had cytogenetic markers of both parents, presenting Mendelian inheritance. However, differences in distribution of heterochromatic blocks were observed between the hybrids and the parent strains. Our results allowed the identification of the hybrid strains based on the cytogenetic markers applied, reinforcing the efficiency of cytogenetic markers as tools for identification and indicating that such events may increase the karyotypic diversity in the genera Astyanax and Hyphessobrycon. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T20:01:57Z 2020-12-10T20:01:57Z 2020-05-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3897/CompCytogen.v14i2.49513 Comparative Cytogenetics. Sofia: Pensoft Publishers, v. 14, n. 2, p. 231-242, 2020. 1993-0771 http://hdl.handle.net/11449/196965 10.3897/CompCytogen.v14i2.49513 WOS:000539067300001 |
url |
http://dx.doi.org/10.3897/CompCytogen.v14i2.49513 http://hdl.handle.net/11449/196965 |
identifier_str_mv |
Comparative Cytogenetics. Sofia: Pensoft Publishers, v. 14, n. 2, p. 231-242, 2020. 1993-0771 10.3897/CompCytogen.v14i2.49513 WOS:000539067300001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Cytogenetics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
231-242 |
dc.publisher.none.fl_str_mv |
Pensoft Publishers |
publisher.none.fl_str_mv |
Pensoft Publishers |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803046287436677120 |