Molecular cytogenetic analysis and the establishment of a cell culture in the fish species Hollandichthys multifasciatus (Eigenmann & Norris, 1900) (Characiformes, Characidae)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-4685-GMB-2020-0260 http://hdl.handle.net/11449/210259 |
Resumo: | Hollandichthys is a fish genus of the family Characidae that was until recently considered to be monotypic, with cytogenetic, morphological, and molecular data being restricted to a few local populations. In the present study, the karyotype of a population of Hollandichthys multifasciatus was analyzed using classical and molecular cytogenetic approaches for the investigation of potential markers that could provide new perspectives on the cytotaxonomy. H. multifasciatus presented a diploid number of 2n=50 chromosomes and a karyotype formula of 8m+10sm+32st. A single pair of chromosomes presented Ag-NORs signals, which coincided with the 18S rDNA sites visualized by FISH, whilst the 5S rDNA sequences were mapped in two chromosome pairs. The distribution of the U snRNA genes was mapped on the Hollandichthys chromosomes for the first time, with the probes revealing the presence of the U1 snDNA on the chromosomes of pair 20, U2 on pairs 6 and 19, U4 on pair 16, and U6 on the chromosomes of pair 11. The results of the present study indicated karyotypic differences in comparison with the other populations of H. multifasciatus studied previously, reinforcing the need for further research to identify isolated populations or the potential existence of cryptic Hollandichthys species. |
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Molecular cytogenetic analysis and the establishment of a cell culture in the fish species Hollandichthys multifasciatus (Eigenmann & Norris, 1900) (Characiformes, Characidae)Neotropical fishcell culturechromosomesFISHrepetitive DNAsHollandichthys is a fish genus of the family Characidae that was until recently considered to be monotypic, with cytogenetic, morphological, and molecular data being restricted to a few local populations. In the present study, the karyotype of a population of Hollandichthys multifasciatus was analyzed using classical and molecular cytogenetic approaches for the investigation of potential markers that could provide new perspectives on the cytotaxonomy. H. multifasciatus presented a diploid number of 2n=50 chromosomes and a karyotype formula of 8m+10sm+32st. A single pair of chromosomes presented Ag-NORs signals, which coincided with the 18S rDNA sites visualized by FISH, whilst the 5S rDNA sequences were mapped in two chromosome pairs. The distribution of the U snRNA genes was mapped on the Hollandichthys chromosomes for the first time, with the probes revealing the presence of the U1 snDNA on the chromosomes of pair 20, U2 on pairs 6 and 19, U4 on pair 16, and U6 on the chromosomes of pair 11. The results of the present study indicated karyotypic differences in comparison with the other populations of H. multifasciatus studied previously, reinforcing the need for further research to identify isolated populations or the potential existence of cryptic Hollandichthys species.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Inst Biociencias, Lab Biol & Genet Peixes, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Lab Biol & Genet Peixes, Botucatu, SP, BrazilFAPESP: 2018/20610-1FAPESP: 2016/09204-6FAPESP: 2014/26508-3CNPq: 306054/2006-0Soc Brasil GeneticaUniversidade Estadual Paulista (Unesp)Soares, Leticia Batista [UNESP]Paim, Fabilene Gomes [UNESP]Ramos, Lucas Peres [UNESP]Foresti, Fausto [UNESP]Oliveira, Claudio [UNESP]2021-06-25T15:02:56Z2021-06-25T15:02:56Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://dx.doi.org/10.1590/1678-4685-GMB-2020-0260Genetics And Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 44, n. 2, 7 p., 2021.1415-4757http://hdl.handle.net/11449/21025910.1590/1678-4685-GMB-2020-0260S1415-47572021000300207WOS:000642407600001S1415-47572021000300207.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics And Molecular Biologyinfo:eu-repo/semantics/openAccess2024-01-17T06:23:04Zoai:repositorio.unesp.br:11449/210259Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:11:53.841808Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular cytogenetic analysis and the establishment of a cell culture in the fish species Hollandichthys multifasciatus (Eigenmann & Norris, 1900) (Characiformes, Characidae) |
title |
Molecular cytogenetic analysis and the establishment of a cell culture in the fish species Hollandichthys multifasciatus (Eigenmann & Norris, 1900) (Characiformes, Characidae) |
spellingShingle |
Molecular cytogenetic analysis and the establishment of a cell culture in the fish species Hollandichthys multifasciatus (Eigenmann & Norris, 1900) (Characiformes, Characidae) Soares, Leticia Batista [UNESP] Neotropical fish cell culture chromosomes FISH repetitive DNAs |
title_short |
Molecular cytogenetic analysis and the establishment of a cell culture in the fish species Hollandichthys multifasciatus (Eigenmann & Norris, 1900) (Characiformes, Characidae) |
title_full |
Molecular cytogenetic analysis and the establishment of a cell culture in the fish species Hollandichthys multifasciatus (Eigenmann & Norris, 1900) (Characiformes, Characidae) |
title_fullStr |
Molecular cytogenetic analysis and the establishment of a cell culture in the fish species Hollandichthys multifasciatus (Eigenmann & Norris, 1900) (Characiformes, Characidae) |
title_full_unstemmed |
Molecular cytogenetic analysis and the establishment of a cell culture in the fish species Hollandichthys multifasciatus (Eigenmann & Norris, 1900) (Characiformes, Characidae) |
title_sort |
Molecular cytogenetic analysis and the establishment of a cell culture in the fish species Hollandichthys multifasciatus (Eigenmann & Norris, 1900) (Characiformes, Characidae) |
author |
Soares, Leticia Batista [UNESP] |
author_facet |
Soares, Leticia Batista [UNESP] Paim, Fabilene Gomes [UNESP] Ramos, Lucas Peres [UNESP] Foresti, Fausto [UNESP] Oliveira, Claudio [UNESP] |
author_role |
author |
author2 |
Paim, Fabilene Gomes [UNESP] Ramos, Lucas Peres [UNESP] Foresti, Fausto [UNESP] Oliveira, Claudio [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Soares, Leticia Batista [UNESP] Paim, Fabilene Gomes [UNESP] Ramos, Lucas Peres [UNESP] Foresti, Fausto [UNESP] Oliveira, Claudio [UNESP] |
dc.subject.por.fl_str_mv |
Neotropical fish cell culture chromosomes FISH repetitive DNAs |
topic |
Neotropical fish cell culture chromosomes FISH repetitive DNAs |
description |
Hollandichthys is a fish genus of the family Characidae that was until recently considered to be monotypic, with cytogenetic, morphological, and molecular data being restricted to a few local populations. In the present study, the karyotype of a population of Hollandichthys multifasciatus was analyzed using classical and molecular cytogenetic approaches for the investigation of potential markers that could provide new perspectives on the cytotaxonomy. H. multifasciatus presented a diploid number of 2n=50 chromosomes and a karyotype formula of 8m+10sm+32st. A single pair of chromosomes presented Ag-NORs signals, which coincided with the 18S rDNA sites visualized by FISH, whilst the 5S rDNA sequences were mapped in two chromosome pairs. The distribution of the U snRNA genes was mapped on the Hollandichthys chromosomes for the first time, with the probes revealing the presence of the U1 snDNA on the chromosomes of pair 20, U2 on pairs 6 and 19, U4 on pair 16, and U6 on the chromosomes of pair 11. The results of the present study indicated karyotypic differences in comparison with the other populations of H. multifasciatus studied previously, reinforcing the need for further research to identify isolated populations or the potential existence of cryptic Hollandichthys species. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T15:02:56Z 2021-06-25T15:02:56Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-4685-GMB-2020-0260 Genetics And Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 44, n. 2, 7 p., 2021. 1415-4757 http://hdl.handle.net/11449/210259 10.1590/1678-4685-GMB-2020-0260 S1415-47572021000300207 WOS:000642407600001 S1415-47572021000300207.pdf |
url |
http://dx.doi.org/10.1590/1678-4685-GMB-2020-0260 http://hdl.handle.net/11449/210259 |
identifier_str_mv |
Genetics And Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 44, n. 2, 7 p., 2021. 1415-4757 10.1590/1678-4685-GMB-2020-0260 S1415-47572021000300207 WOS:000642407600001 S1415-47572021000300207.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics And Molecular Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasil Genetica |
publisher.none.fl_str_mv |
Soc Brasil Genetica |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129498596507648 |