Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.carbpol.2019.115203 http://hdl.handle.net/11449/186171 |
Resumo: | A fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects. FMG-Hm was not cytotoxic, nor did it alter the cell morphology and proliferation, but was able to inhibit colony-forming ability and cell migration in B16-F10 murine melanoma cells. An analysis of the monosaccharide composition indicated that FMG-Hm was composed of fucose, mannose, and galactose in a ratio of 1.00:1.08:3.17. The FMG-Hm was structurally characterized based on methylation analysis, partial acid hydrolysis, and NMR experiments. The results indicated that FMG-Hm contained a alpha-(1 -> 6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of alpha-L-fucopyranose and beta-D-mannopyranose. The predicted structure of the heteropolysaccharide was established as: [GRAPHICS] . |
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Repositório Institucional da UNESP |
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Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreusHypsizygus marmoreusMushroomFucomannogalactanChemical structureAntimelanomaB16-F10 cellA fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects. FMG-Hm was not cytotoxic, nor did it alter the cell morphology and proliferation, but was able to inhibit colony-forming ability and cell migration in B16-F10 murine melanoma cells. An analysis of the monosaccharide composition indicated that FMG-Hm was composed of fucose, mannose, and galactose in a ratio of 1.00:1.08:3.17. The FMG-Hm was structurally characterized based on methylation analysis, partial acid hydrolysis, and NMR experiments. The results indicated that FMG-Hm contained a alpha-(1 -> 6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of alpha-L-fucopyranose and beta-D-mannopyranose. The predicted structure of the heteropolysaccharide was established as: [GRAPHICS] .Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Goias, Dept Quim, BR-75704020 Regional Catalao, Catalao, BrazilUniv Fed Parana, Dept Biol Celular, BR-81531980 Curitiba, Parana, BrazilUniv Estadual Paulista, Fac Ciencias Agron, Dept Engn Rural, BR-18610307 Botucatu, SP, BrazilUniv Fed Goias, Inst Quim, Lab Ressonancia Magnet Nucl, Campus Samambaia, BR-74001970 Goiania, Go, BrazilUniv Estadual Paulista, Fac Ciencias & Tecnol, Dept Quim & Bioquim, BR-19060900 Presidente Prudente, BrazilUniv Estadual Paulista, Fac Ciencias Agron, Dept Engn Rural, BR-18610307 Botucatu, SP, BrazilUniv Estadual Paulista, Fac Ciencias & Tecnol, Dept Quim & Bioquim, BR-19060900 Presidente Prudente, BrazilCAPES: 001Elsevier B.V.Universidade Federal de Goiás (UFG)Univ Fed ParanaUniversidade Estadual Paulista (Unesp)Oliveira, Ruberney S.Biscaia, Stellee M. P.Bellan, Daniel L.Viana, Sthefany R. F. [UNESP]Di-Medeiros Leal, Maria CarolinaVasconcelos, Ana Flora D. [UNESP]Liao, Luciano M.Trindade, Edvaldo S.Carbonero, Elaine R.2019-10-04T12:42:23Z2019-10-04T12:42:23Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9http://dx.doi.org/10.1016/j.carbpol.2019.115203Carbohydrate Polymers. Oxford: Elsevier Sci Ltd, v. 225, 9 p., 2019.0144-8617http://hdl.handle.net/11449/18617110.1016/j.carbpol.2019.115203WOS:000485109100052Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCarbohydrate Polymersinfo:eu-repo/semantics/openAccess2024-06-19T12:44:01Zoai:repositorio.unesp.br:11449/186171Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:35:00.311871Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus |
title |
Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus |
spellingShingle |
Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus Oliveira, Ruberney S. Hypsizygus marmoreus Mushroom Fucomannogalactan Chemical structure Antimelanoma B16-F10 cell |
title_short |
Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus |
title_full |
Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus |
title_fullStr |
Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus |
title_full_unstemmed |
Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus |
title_sort |
Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus |
author |
Oliveira, Ruberney S. |
author_facet |
Oliveira, Ruberney S. Biscaia, Stellee M. P. Bellan, Daniel L. Viana, Sthefany R. F. [UNESP] Di-Medeiros Leal, Maria Carolina Vasconcelos, Ana Flora D. [UNESP] Liao, Luciano M. Trindade, Edvaldo S. Carbonero, Elaine R. |
author_role |
author |
author2 |
Biscaia, Stellee M. P. Bellan, Daniel L. Viana, Sthefany R. F. [UNESP] Di-Medeiros Leal, Maria Carolina Vasconcelos, Ana Flora D. [UNESP] Liao, Luciano M. Trindade, Edvaldo S. Carbonero, Elaine R. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Goiás (UFG) Univ Fed Parana Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Oliveira, Ruberney S. Biscaia, Stellee M. P. Bellan, Daniel L. Viana, Sthefany R. F. [UNESP] Di-Medeiros Leal, Maria Carolina Vasconcelos, Ana Flora D. [UNESP] Liao, Luciano M. Trindade, Edvaldo S. Carbonero, Elaine R. |
dc.subject.por.fl_str_mv |
Hypsizygus marmoreus Mushroom Fucomannogalactan Chemical structure Antimelanoma B16-F10 cell |
topic |
Hypsizygus marmoreus Mushroom Fucomannogalactan Chemical structure Antimelanoma B16-F10 cell |
description |
A fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects. FMG-Hm was not cytotoxic, nor did it alter the cell morphology and proliferation, but was able to inhibit colony-forming ability and cell migration in B16-F10 murine melanoma cells. An analysis of the monosaccharide composition indicated that FMG-Hm was composed of fucose, mannose, and galactose in a ratio of 1.00:1.08:3.17. The FMG-Hm was structurally characterized based on methylation analysis, partial acid hydrolysis, and NMR experiments. The results indicated that FMG-Hm contained a alpha-(1 -> 6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of alpha-L-fucopyranose and beta-D-mannopyranose. The predicted structure of the heteropolysaccharide was established as: [GRAPHICS] . |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:42:23Z 2019-10-04T12:42:23Z 2019-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.carbpol.2019.115203 Carbohydrate Polymers. Oxford: Elsevier Sci Ltd, v. 225, 9 p., 2019. 0144-8617 http://hdl.handle.net/11449/186171 10.1016/j.carbpol.2019.115203 WOS:000485109100052 |
url |
http://dx.doi.org/10.1016/j.carbpol.2019.115203 http://hdl.handle.net/11449/186171 |
identifier_str_mv |
Carbohydrate Polymers. Oxford: Elsevier Sci Ltd, v. 225, 9 p., 2019. 0144-8617 10.1016/j.carbpol.2019.115203 WOS:000485109100052 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Carbohydrate Polymers |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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_version_ |
1808129532089073664 |