Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus

• Autor(a) principal: Oliveira, Ruberney S.
• Data de Publicação: 2019
• Outros Autores: Biscaia, Stellee M. P., Bellan, Daniel L., Viana, Sthefany R. F. [UNESP], Di-Medeiros Leal, Maria Carolina, Vasconcelos, Ana Flora D. [UNESP], Liao, Luciano M., Trindade, Edvaldo S., Carbonero, Elaine R.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.carbpol.2019.115203; http://hdl.handle.net/11449/186171
• topic: Hypsizygus marmoreus; Mushroom; Fucomannogalactan; Chemical structure; Antimelanoma; B16-F10 cell

A fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects. FMG-Hm was not cytotoxic, nor did it alter the cell morphology and proliferation, but was able to inhibit colony-forming ability and cell migration in B16-F10 murine melanoma cells. An analysis of the monosaccharide composition indicated that FMG-Hm was composed of fucose, mannose, and galactose in a ratio of 1.00:1.08:3.17. The FMG-Hm was structurally characterized based on methylation analysis, partial acid hydrolysis, and NMR experiments. The results indicated that FMG-Hm contained a alpha-(1 -> 6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of alpha-L-fucopyranose and beta-D-mannopyranose. The predicted structure of the heteropolysaccharide was established as: [GRAPHICS] .