The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vetpar.2011.07.051 http://hdl.handle.net/11449/18983 |
Resumo: | The indiscriminate use of anthelmintics has resulted in the establishment of parasite resistance. Thus, this study aimed to evaluate the in vitro antiparasitic effect of plant extracts on Haemonchus contortus in sheep and the in vivo effect on Strongyloides venezuelensis in Rattus norvegicus. The plant extracts from Piper tuberculatum, Lippia sidoides, Mentha piperita, Hura crepitans and Carapa guianensis, produced at different research institutions, were chemically analyzed and evaluated through the egg hatch test (EHT) and larval development test (LDT) in H. contortus. P. tuberculatum (150 and 250 mg kg(-1) of body weight) was evaluated for its anthelmintic action on R. norvegicus experimentally infected with S. venezuelensis. In the EHT, the LC50 and LC90 of the extracts were respectively as follows: 0.031 and 0.09 mg mL(-1) for P. tuberculatum, 0.04 and 0.13 mg mL(-1) for L sidoides, 0.037 and 0.10 mg mL(-1) for M. piperita, 2.16 and 17.13 mg mL(-1) for H. crepitans and 2.03 x 10(-6) and 1.22 x 10(-12) mg mL(-1) for C guianensis. In the LDT, the LC50 and LC90 were respectively: 0.02 and 0.031 mg mL(-1) for P. tuberculatum, 0.002 and 0.04 mg mL(-1) for L. sidoides, 0.018 and 0.03 mg mL(-1) for M. piperita, 0.36 and 0.91 mg mL(-1) for H. crepitans and 17.65 and 1890 mg mL(-1) for C. guianensis. The extract of P. tuberculatum showed the following substances: piperamides as (Z)-piplartine, (E)-piplartine, 8,9-dihydropiplartine, piperine, 10,11-dihydropiperine, 5,6 dihydropiperlongumine and pellitorine. The major compounds of the oils were thymol (76.6%) for L sidoides, menthol (27.5%) for M. piperita and oleic acid (46.8%) for C guianensis. Regarding the in vivo test, neither dose of P. tuberculatum caused any significant reduction (P > 0.05) in worm burden and fecal egg counts compared with the control group. We conclude that the extracts of P. tuberculatum, L sidoides and M. piperita have effective activity when tested in vitro, but the doses of the extract of P. tuberculatum have no effect when employed in in vivo tests. (C) 2011 Elsevier B.V. All rights reserved. |
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The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensisGastrointestinal nematodesFolk medicinePlant extractsControlThe indiscriminate use of anthelmintics has resulted in the establishment of parasite resistance. Thus, this study aimed to evaluate the in vitro antiparasitic effect of plant extracts on Haemonchus contortus in sheep and the in vivo effect on Strongyloides venezuelensis in Rattus norvegicus. The plant extracts from Piper tuberculatum, Lippia sidoides, Mentha piperita, Hura crepitans and Carapa guianensis, produced at different research institutions, were chemically analyzed and evaluated through the egg hatch test (EHT) and larval development test (LDT) in H. contortus. P. tuberculatum (150 and 250 mg kg(-1) of body weight) was evaluated for its anthelmintic action on R. norvegicus experimentally infected with S. venezuelensis. In the EHT, the LC50 and LC90 of the extracts were respectively as follows: 0.031 and 0.09 mg mL(-1) for P. tuberculatum, 0.04 and 0.13 mg mL(-1) for L sidoides, 0.037 and 0.10 mg mL(-1) for M. piperita, 2.16 and 17.13 mg mL(-1) for H. crepitans and 2.03 x 10(-6) and 1.22 x 10(-12) mg mL(-1) for C guianensis. In the LDT, the LC50 and LC90 were respectively: 0.02 and 0.031 mg mL(-1) for P. tuberculatum, 0.002 and 0.04 mg mL(-1) for L. sidoides, 0.018 and 0.03 mg mL(-1) for M. piperita, 0.36 and 0.91 mg mL(-1) for H. crepitans and 17.65 and 1890 mg mL(-1) for C. guianensis. The extract of P. tuberculatum showed the following substances: piperamides as (Z)-piplartine, (E)-piplartine, 8,9-dihydropiplartine, piperine, 10,11-dihydropiperine, 5,6 dihydropiperlongumine and pellitorine. The major compounds of the oils were thymol (76.6%) for L sidoides, menthol (27.5%) for M. piperita and oleic acid (46.8%) for C guianensis. Regarding the in vivo test, neither dose of P. tuberculatum caused any significant reduction (P > 0.05) in worm burden and fecal egg counts compared with the control group. We conclude that the extracts of P. tuberculatum, L sidoides and M. piperita have effective activity when tested in vitro, but the doses of the extract of P. tuberculatum have no effect when employed in in vivo tests. (C) 2011 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Parasitol, Inst Biociencias, BR-18618000 Botucatu, SP, BrazilEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Pecuaria Sudeste, São Carlos, SP, BrazilUniv Estadual Paulista, Dept Quim Organ, Inst Quim, Araraquara, SP, BrazilEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Rondonia, Porto Velho, RO, BrazilEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Amazonia Ocidental, Manaus, AM, BrazilEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Agroind Alimentos, Rio de Janeiro, RJ, BrazilUniv Estadual Paulista, Dept Parasitol, Inst Biociencias, BR-18618000 Botucatu, SP, BrazilUniv Estadual Paulista, Dept Quim Organ, Inst Quim, Araraquara, SP, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Carvalho, Camila O. [UNESP]Chagas, Ana Carolina S.Cotinguiba, Fernando [UNESP]Furlan, Maysa [UNESP]Brito, Luciana G.Chaves, Francisco C. M.Stephan, Marilia P.Bizzo, Humberto R.Amarante, Alessandro Francisco Talamini do [UNESP]2014-05-20T13:53:13Z2014-05-20T13:53:13Z2012-02-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article260-268application/pdfhttp://dx.doi.org/10.1016/j.vetpar.2011.07.051Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 183, n. 3-4, p. 260-268, 2012.0304-4017http://hdl.handle.net/11449/1898310.1016/j.vetpar.2011.07.051WOS:000299458100009WOS000299458100009.pdf26772316633297061308042794786872Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Parasitology2.4221,275info:eu-repo/semantics/openAccess2023-12-11T06:12:57Zoai:repositorio.unesp.br:11449/18983Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:00:21.481771Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis |
title |
The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis |
spellingShingle |
The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis Carvalho, Camila O. [UNESP] Gastrointestinal nematodes Folk medicine Plant extracts Control |
title_short |
The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis |
title_full |
The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis |
title_fullStr |
The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis |
title_full_unstemmed |
The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis |
title_sort |
The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis |
author |
Carvalho, Camila O. [UNESP] |
author_facet |
Carvalho, Camila O. [UNESP] Chagas, Ana Carolina S. Cotinguiba, Fernando [UNESP] Furlan, Maysa [UNESP] Brito, Luciana G. Chaves, Francisco C. M. Stephan, Marilia P. Bizzo, Humberto R. Amarante, Alessandro Francisco Talamini do [UNESP] |
author_role |
author |
author2 |
Chagas, Ana Carolina S. Cotinguiba, Fernando [UNESP] Furlan, Maysa [UNESP] Brito, Luciana G. Chaves, Francisco C. M. Stephan, Marilia P. Bizzo, Humberto R. Amarante, Alessandro Francisco Talamini do [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Carvalho, Camila O. [UNESP] Chagas, Ana Carolina S. Cotinguiba, Fernando [UNESP] Furlan, Maysa [UNESP] Brito, Luciana G. Chaves, Francisco C. M. Stephan, Marilia P. Bizzo, Humberto R. Amarante, Alessandro Francisco Talamini do [UNESP] |
dc.subject.por.fl_str_mv |
Gastrointestinal nematodes Folk medicine Plant extracts Control |
topic |
Gastrointestinal nematodes Folk medicine Plant extracts Control |
description |
The indiscriminate use of anthelmintics has resulted in the establishment of parasite resistance. Thus, this study aimed to evaluate the in vitro antiparasitic effect of plant extracts on Haemonchus contortus in sheep and the in vivo effect on Strongyloides venezuelensis in Rattus norvegicus. The plant extracts from Piper tuberculatum, Lippia sidoides, Mentha piperita, Hura crepitans and Carapa guianensis, produced at different research institutions, were chemically analyzed and evaluated through the egg hatch test (EHT) and larval development test (LDT) in H. contortus. P. tuberculatum (150 and 250 mg kg(-1) of body weight) was evaluated for its anthelmintic action on R. norvegicus experimentally infected with S. venezuelensis. In the EHT, the LC50 and LC90 of the extracts were respectively as follows: 0.031 and 0.09 mg mL(-1) for P. tuberculatum, 0.04 and 0.13 mg mL(-1) for L sidoides, 0.037 and 0.10 mg mL(-1) for M. piperita, 2.16 and 17.13 mg mL(-1) for H. crepitans and 2.03 x 10(-6) and 1.22 x 10(-12) mg mL(-1) for C guianensis. In the LDT, the LC50 and LC90 were respectively: 0.02 and 0.031 mg mL(-1) for P. tuberculatum, 0.002 and 0.04 mg mL(-1) for L. sidoides, 0.018 and 0.03 mg mL(-1) for M. piperita, 0.36 and 0.91 mg mL(-1) for H. crepitans and 17.65 and 1890 mg mL(-1) for C. guianensis. The extract of P. tuberculatum showed the following substances: piperamides as (Z)-piplartine, (E)-piplartine, 8,9-dihydropiplartine, piperine, 10,11-dihydropiperine, 5,6 dihydropiperlongumine and pellitorine. The major compounds of the oils were thymol (76.6%) for L sidoides, menthol (27.5%) for M. piperita and oleic acid (46.8%) for C guianensis. Regarding the in vivo test, neither dose of P. tuberculatum caused any significant reduction (P > 0.05) in worm burden and fecal egg counts compared with the control group. We conclude that the extracts of P. tuberculatum, L sidoides and M. piperita have effective activity when tested in vitro, but the doses of the extract of P. tuberculatum have no effect when employed in in vivo tests. (C) 2011 Elsevier B.V. All rights reserved. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-02-10 2014-05-20T13:53:13Z 2014-05-20T13:53:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vetpar.2011.07.051 Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 183, n. 3-4, p. 260-268, 2012. 0304-4017 http://hdl.handle.net/11449/18983 10.1016/j.vetpar.2011.07.051 WOS:000299458100009 WOS000299458100009.pdf 2677231663329706 1308042794786872 |
url |
http://dx.doi.org/10.1016/j.vetpar.2011.07.051 http://hdl.handle.net/11449/18983 |
identifier_str_mv |
Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 183, n. 3-4, p. 260-268, 2012. 0304-4017 10.1016/j.vetpar.2011.07.051 WOS:000299458100009 WOS000299458100009.pdf 2677231663329706 1308042794786872 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Parasitology 2.422 1,275 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
260-268 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129148681453568 |