The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis

Detalhes bibliográficos
Autor(a) principal: Carvalho, Camila O. [UNESP]
Data de Publicação: 2012
Outros Autores: Chagas, Ana Carolina S., Cotinguiba, Fernando [UNESP], Furlan, Maysa [UNESP], Brito, Luciana G., Chaves, Francisco C. M., Stephan, Marilia P., Bizzo, Humberto R., Amarante, Alessandro Francisco Talamini do [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.vetpar.2011.07.051
http://hdl.handle.net/11449/18983
Resumo: The indiscriminate use of anthelmintics has resulted in the establishment of parasite resistance. Thus, this study aimed to evaluate the in vitro antiparasitic effect of plant extracts on Haemonchus contortus in sheep and the in vivo effect on Strongyloides venezuelensis in Rattus norvegicus. The plant extracts from Piper tuberculatum, Lippia sidoides, Mentha piperita, Hura crepitans and Carapa guianensis, produced at different research institutions, were chemically analyzed and evaluated through the egg hatch test (EHT) and larval development test (LDT) in H. contortus. P. tuberculatum (150 and 250 mg kg(-1) of body weight) was evaluated for its anthelmintic action on R. norvegicus experimentally infected with S. venezuelensis. In the EHT, the LC50 and LC90 of the extracts were respectively as follows: 0.031 and 0.09 mg mL(-1) for P. tuberculatum, 0.04 and 0.13 mg mL(-1) for L sidoides, 0.037 and 0.10 mg mL(-1) for M. piperita, 2.16 and 17.13 mg mL(-1) for H. crepitans and 2.03 x 10(-6) and 1.22 x 10(-12) mg mL(-1) for C guianensis. In the LDT, the LC50 and LC90 were respectively: 0.02 and 0.031 mg mL(-1) for P. tuberculatum, 0.002 and 0.04 mg mL(-1) for L. sidoides, 0.018 and 0.03 mg mL(-1) for M. piperita, 0.36 and 0.91 mg mL(-1) for H. crepitans and 17.65 and 1890 mg mL(-1) for C. guianensis. The extract of P. tuberculatum showed the following substances: piperamides as (Z)-piplartine, (E)-piplartine, 8,9-dihydropiplartine, piperine, 10,11-dihydropiperine, 5,6 dihydropiperlongumine and pellitorine. The major compounds of the oils were thymol (76.6%) for L sidoides, menthol (27.5%) for M. piperita and oleic acid (46.8%) for C guianensis. Regarding the in vivo test, neither dose of P. tuberculatum caused any significant reduction (P > 0.05) in worm burden and fecal egg counts compared with the control group. We conclude that the extracts of P. tuberculatum, L sidoides and M. piperita have effective activity when tested in vitro, but the doses of the extract of P. tuberculatum have no effect when employed in in vivo tests. (C) 2011 Elsevier B.V. All rights reserved.
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spelling The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensisGastrointestinal nematodesFolk medicinePlant extractsControlThe indiscriminate use of anthelmintics has resulted in the establishment of parasite resistance. Thus, this study aimed to evaluate the in vitro antiparasitic effect of plant extracts on Haemonchus contortus in sheep and the in vivo effect on Strongyloides venezuelensis in Rattus norvegicus. The plant extracts from Piper tuberculatum, Lippia sidoides, Mentha piperita, Hura crepitans and Carapa guianensis, produced at different research institutions, were chemically analyzed and evaluated through the egg hatch test (EHT) and larval development test (LDT) in H. contortus. P. tuberculatum (150 and 250 mg kg(-1) of body weight) was evaluated for its anthelmintic action on R. norvegicus experimentally infected with S. venezuelensis. In the EHT, the LC50 and LC90 of the extracts were respectively as follows: 0.031 and 0.09 mg mL(-1) for P. tuberculatum, 0.04 and 0.13 mg mL(-1) for L sidoides, 0.037 and 0.10 mg mL(-1) for M. piperita, 2.16 and 17.13 mg mL(-1) for H. crepitans and 2.03 x 10(-6) and 1.22 x 10(-12) mg mL(-1) for C guianensis. In the LDT, the LC50 and LC90 were respectively: 0.02 and 0.031 mg mL(-1) for P. tuberculatum, 0.002 and 0.04 mg mL(-1) for L. sidoides, 0.018 and 0.03 mg mL(-1) for M. piperita, 0.36 and 0.91 mg mL(-1) for H. crepitans and 17.65 and 1890 mg mL(-1) for C. guianensis. The extract of P. tuberculatum showed the following substances: piperamides as (Z)-piplartine, (E)-piplartine, 8,9-dihydropiplartine, piperine, 10,11-dihydropiperine, 5,6 dihydropiperlongumine and pellitorine. The major compounds of the oils were thymol (76.6%) for L sidoides, menthol (27.5%) for M. piperita and oleic acid (46.8%) for C guianensis. Regarding the in vivo test, neither dose of P. tuberculatum caused any significant reduction (P > 0.05) in worm burden and fecal egg counts compared with the control group. We conclude that the extracts of P. tuberculatum, L sidoides and M. piperita have effective activity when tested in vitro, but the doses of the extract of P. tuberculatum have no effect when employed in in vivo tests. (C) 2011 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Parasitol, Inst Biociencias, BR-18618000 Botucatu, SP, BrazilEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Pecuaria Sudeste, São Carlos, SP, BrazilUniv Estadual Paulista, Dept Quim Organ, Inst Quim, Araraquara, SP, BrazilEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Rondonia, Porto Velho, RO, BrazilEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Amazonia Ocidental, Manaus, AM, BrazilEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Agroind Alimentos, Rio de Janeiro, RJ, BrazilUniv Estadual Paulista, Dept Parasitol, Inst Biociencias, BR-18618000 Botucatu, SP, BrazilUniv Estadual Paulista, Dept Quim Organ, Inst Quim, Araraquara, SP, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Carvalho, Camila O. [UNESP]Chagas, Ana Carolina S.Cotinguiba, Fernando [UNESP]Furlan, Maysa [UNESP]Brito, Luciana G.Chaves, Francisco C. M.Stephan, Marilia P.Bizzo, Humberto R.Amarante, Alessandro Francisco Talamini do [UNESP]2014-05-20T13:53:13Z2014-05-20T13:53:13Z2012-02-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article260-268application/pdfhttp://dx.doi.org/10.1016/j.vetpar.2011.07.051Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 183, n. 3-4, p. 260-268, 2012.0304-4017http://hdl.handle.net/11449/1898310.1016/j.vetpar.2011.07.051WOS:000299458100009WOS000299458100009.pdf26772316633297061308042794786872Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Parasitology2.4221,275info:eu-repo/semantics/openAccess2023-12-11T06:12:57Zoai:repositorio.unesp.br:11449/18983Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:00:21.481771Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis
title The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis
spellingShingle The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis
Carvalho, Camila O. [UNESP]
Gastrointestinal nematodes
Folk medicine
Plant extracts
Control
title_short The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis
title_full The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis
title_fullStr The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis
title_full_unstemmed The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis
title_sort The anthelmintic effect of plant extracts on Haemonchus contortus and Strongyloides venezuelensis
author Carvalho, Camila O. [UNESP]
author_facet Carvalho, Camila O. [UNESP]
Chagas, Ana Carolina S.
Cotinguiba, Fernando [UNESP]
Furlan, Maysa [UNESP]
Brito, Luciana G.
Chaves, Francisco C. M.
Stephan, Marilia P.
Bizzo, Humberto R.
Amarante, Alessandro Francisco Talamini do [UNESP]
author_role author
author2 Chagas, Ana Carolina S.
Cotinguiba, Fernando [UNESP]
Furlan, Maysa [UNESP]
Brito, Luciana G.
Chaves, Francisco C. M.
Stephan, Marilia P.
Bizzo, Humberto R.
Amarante, Alessandro Francisco Talamini do [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Carvalho, Camila O. [UNESP]
Chagas, Ana Carolina S.
Cotinguiba, Fernando [UNESP]
Furlan, Maysa [UNESP]
Brito, Luciana G.
Chaves, Francisco C. M.
Stephan, Marilia P.
Bizzo, Humberto R.
Amarante, Alessandro Francisco Talamini do [UNESP]
dc.subject.por.fl_str_mv Gastrointestinal nematodes
Folk medicine
Plant extracts
Control
topic Gastrointestinal nematodes
Folk medicine
Plant extracts
Control
description The indiscriminate use of anthelmintics has resulted in the establishment of parasite resistance. Thus, this study aimed to evaluate the in vitro antiparasitic effect of plant extracts on Haemonchus contortus in sheep and the in vivo effect on Strongyloides venezuelensis in Rattus norvegicus. The plant extracts from Piper tuberculatum, Lippia sidoides, Mentha piperita, Hura crepitans and Carapa guianensis, produced at different research institutions, were chemically analyzed and evaluated through the egg hatch test (EHT) and larval development test (LDT) in H. contortus. P. tuberculatum (150 and 250 mg kg(-1) of body weight) was evaluated for its anthelmintic action on R. norvegicus experimentally infected with S. venezuelensis. In the EHT, the LC50 and LC90 of the extracts were respectively as follows: 0.031 and 0.09 mg mL(-1) for P. tuberculatum, 0.04 and 0.13 mg mL(-1) for L sidoides, 0.037 and 0.10 mg mL(-1) for M. piperita, 2.16 and 17.13 mg mL(-1) for H. crepitans and 2.03 x 10(-6) and 1.22 x 10(-12) mg mL(-1) for C guianensis. In the LDT, the LC50 and LC90 were respectively: 0.02 and 0.031 mg mL(-1) for P. tuberculatum, 0.002 and 0.04 mg mL(-1) for L. sidoides, 0.018 and 0.03 mg mL(-1) for M. piperita, 0.36 and 0.91 mg mL(-1) for H. crepitans and 17.65 and 1890 mg mL(-1) for C. guianensis. The extract of P. tuberculatum showed the following substances: piperamides as (Z)-piplartine, (E)-piplartine, 8,9-dihydropiplartine, piperine, 10,11-dihydropiperine, 5,6 dihydropiperlongumine and pellitorine. The major compounds of the oils were thymol (76.6%) for L sidoides, menthol (27.5%) for M. piperita and oleic acid (46.8%) for C guianensis. Regarding the in vivo test, neither dose of P. tuberculatum caused any significant reduction (P > 0.05) in worm burden and fecal egg counts compared with the control group. We conclude that the extracts of P. tuberculatum, L sidoides and M. piperita have effective activity when tested in vitro, but the doses of the extract of P. tuberculatum have no effect when employed in in vivo tests. (C) 2011 Elsevier B.V. All rights reserved.
publishDate 2012
dc.date.none.fl_str_mv 2012-02-10
2014-05-20T13:53:13Z
2014-05-20T13:53:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.vetpar.2011.07.051
Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 183, n. 3-4, p. 260-268, 2012.
0304-4017
http://hdl.handle.net/11449/18983
10.1016/j.vetpar.2011.07.051
WOS:000299458100009
WOS000299458100009.pdf
2677231663329706
1308042794786872
url http://dx.doi.org/10.1016/j.vetpar.2011.07.051
http://hdl.handle.net/11449/18983
identifier_str_mv Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 183, n. 3-4, p. 260-268, 2012.
0304-4017
10.1016/j.vetpar.2011.07.051
WOS:000299458100009
WOS000299458100009.pdf
2677231663329706
1308042794786872
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary Parasitology
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1,275
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 260-268
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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