Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.micapath.2018.03.050 http://hdl.handle.net/11449/164353 |
Resumo: | Mast cells (MCs) have important immunoregulatory roles in skin inflammation. Annexin A1 (ANXA1) is an endogenous anti-inflammatory protein that can be expressed by mast cells, neutrophils, eosinophils, monocytes, epithelial and T cells. This study investigated MCs heterogeneity and ANXA1 expression in human dermatoses with special emphasis in leprosy. Sixty one skin biopsies from 2 groups were investigated: 40 newly diagnosed untreated leprosy patients (18 reaction-free, 11 type 1 reaction/T1R, 11 type 2 reaction/T2R); 21 patients with other dermatoses. Tryptase/try + and chymase/chy + phenotypic markers and toluidine blue stained intact/degranulated MC counts/mm(2) were evaluated. Try(+)/chy(+) MCs and ANXA1 were identified by streptavidin-biotin-peroxidase immunostaining and density was reported. In leprosy, degranulated MCs outnumbered intact ones regardless of the leprosy form (from tuberculoid/TT to lepromatous/LL), leprosy reactions (reactional/reaction-free) and type of reaction (T1R/T2R). Compared to other dermatoses, leprosy skin lesions showed lower numbers of degranulated and intact MCs. Try(+) MCs outnumbered chy(+) in leprosy lesions (reaction-free/reactional, particularly in T2R), but not in other dermatoses. Compared to other dermatoses, ANXA1 expression, which is also expressed in mast cells, was higher in the epidermis of leprosy skin lesions, independently of reactional episode. In leprosy, higher MC degranulation and differential expression of try(+)/chy(+) subsets independent of leprosy type and reaction suggest that the Mycobacterium leprae infection itself dictates the inflammatory MCs activation in skin lesions. Higher expression of ANXA1 in leprosy suggests its potential anti-inflammatory role to maintain homeostasis preventing tissue and nerve damage. |
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Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesionsMast cellAnnexin A1TryptaseChymaseLeprosySkin diseasesMast cells (MCs) have important immunoregulatory roles in skin inflammation. Annexin A1 (ANXA1) is an endogenous anti-inflammatory protein that can be expressed by mast cells, neutrophils, eosinophils, monocytes, epithelial and T cells. This study investigated MCs heterogeneity and ANXA1 expression in human dermatoses with special emphasis in leprosy. Sixty one skin biopsies from 2 groups were investigated: 40 newly diagnosed untreated leprosy patients (18 reaction-free, 11 type 1 reaction/T1R, 11 type 2 reaction/T2R); 21 patients with other dermatoses. Tryptase/try + and chymase/chy + phenotypic markers and toluidine blue stained intact/degranulated MC counts/mm(2) were evaluated. Try(+)/chy(+) MCs and ANXA1 were identified by streptavidin-biotin-peroxidase immunostaining and density was reported. In leprosy, degranulated MCs outnumbered intact ones regardless of the leprosy form (from tuberculoid/TT to lepromatous/LL), leprosy reactions (reactional/reaction-free) and type of reaction (T1R/T2R). Compared to other dermatoses, leprosy skin lesions showed lower numbers of degranulated and intact MCs. Try(+) MCs outnumbered chy(+) in leprosy lesions (reaction-free/reactional, particularly in T2R), but not in other dermatoses. Compared to other dermatoses, ANXA1 expression, which is also expressed in mast cells, was higher in the epidermis of leprosy skin lesions, independently of reactional episode. In leprosy, higher MC degranulation and differential expression of try(+)/chy(+) subsets independent of leprosy type and reaction suggest that the Mycobacterium leprae infection itself dictates the inflammatory MCs activation in skin lesions. Higher expression of ANXA1 in leprosy suggests its potential anti-inflammatory role to maintain homeostasis preventing tissue and nerve damage.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Goias, Trop Pathol & Publ Hlth Inst, Rua 235 S-N Setor Univ, BR-74605050 Goiania, Go, BrazilSao Paulo State Univ, UNESP, Dept Biol, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, SP, BrazilSao Paulo State Univ, UNESP, Dept Biol, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, SP, BrazilCNPq: 304869/2008-2CNPq: 308144/2014-7CNPq: 141554/2013-4FAPESP: 2012/21603-2FAPESP: 2012/13041-4CAPES: 1054292Elsevier B.V.Universidade Federal de Goiás (UFG)Universidade Estadual Paulista (Unesp)Costa, Mauricio B.Mimura, Kallyne K. O. [UNESP]Freitas, Aline A.Hungria, Emerith M.Sousa, Ana Lucia O. M.Oliani, Sonia M. [UNESP]Stefani, Mariane M. A.2018-11-26T17:52:15Z2018-11-26T17:52:15Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article277-284application/pdfhttp://dx.doi.org/10.1016/j.micapath.2018.03.050Microbial Pathogenesis. London: Academic Press Ltd- Elsevier Science Ltd, v. 118, p. 277-284, 2018.0882-4010http://hdl.handle.net/11449/16435310.1016/j.micapath.2018.03.050WOS:000436528000038WOS000436528000038.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Pathogenesis0,751info:eu-repo/semantics/openAccess2024-01-29T06:24:11Zoai:repositorio.unesp.br:11449/164353Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:11:28.471346Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions |
title |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions |
spellingShingle |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions Costa, Mauricio B. Mast cell Annexin A1 Tryptase Chymase Leprosy Skin diseases |
title_short |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions |
title_full |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions |
title_fullStr |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions |
title_full_unstemmed |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions |
title_sort |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions |
author |
Costa, Mauricio B. |
author_facet |
Costa, Mauricio B. Mimura, Kallyne K. O. [UNESP] Freitas, Aline A. Hungria, Emerith M. Sousa, Ana Lucia O. M. Oliani, Sonia M. [UNESP] Stefani, Mariane M. A. |
author_role |
author |
author2 |
Mimura, Kallyne K. O. [UNESP] Freitas, Aline A. Hungria, Emerith M. Sousa, Ana Lucia O. M. Oliani, Sonia M. [UNESP] Stefani, Mariane M. A. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Goiás (UFG) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Costa, Mauricio B. Mimura, Kallyne K. O. [UNESP] Freitas, Aline A. Hungria, Emerith M. Sousa, Ana Lucia O. M. Oliani, Sonia M. [UNESP] Stefani, Mariane M. A. |
dc.subject.por.fl_str_mv |
Mast cell Annexin A1 Tryptase Chymase Leprosy Skin diseases |
topic |
Mast cell Annexin A1 Tryptase Chymase Leprosy Skin diseases |
description |
Mast cells (MCs) have important immunoregulatory roles in skin inflammation. Annexin A1 (ANXA1) is an endogenous anti-inflammatory protein that can be expressed by mast cells, neutrophils, eosinophils, monocytes, epithelial and T cells. This study investigated MCs heterogeneity and ANXA1 expression in human dermatoses with special emphasis in leprosy. Sixty one skin biopsies from 2 groups were investigated: 40 newly diagnosed untreated leprosy patients (18 reaction-free, 11 type 1 reaction/T1R, 11 type 2 reaction/T2R); 21 patients with other dermatoses. Tryptase/try + and chymase/chy + phenotypic markers and toluidine blue stained intact/degranulated MC counts/mm(2) were evaluated. Try(+)/chy(+) MCs and ANXA1 were identified by streptavidin-biotin-peroxidase immunostaining and density was reported. In leprosy, degranulated MCs outnumbered intact ones regardless of the leprosy form (from tuberculoid/TT to lepromatous/LL), leprosy reactions (reactional/reaction-free) and type of reaction (T1R/T2R). Compared to other dermatoses, leprosy skin lesions showed lower numbers of degranulated and intact MCs. Try(+) MCs outnumbered chy(+) in leprosy lesions (reaction-free/reactional, particularly in T2R), but not in other dermatoses. Compared to other dermatoses, ANXA1 expression, which is also expressed in mast cells, was higher in the epidermis of leprosy skin lesions, independently of reactional episode. In leprosy, higher MC degranulation and differential expression of try(+)/chy(+) subsets independent of leprosy type and reaction suggest that the Mycobacterium leprae infection itself dictates the inflammatory MCs activation in skin lesions. Higher expression of ANXA1 in leprosy suggests its potential anti-inflammatory role to maintain homeostasis preventing tissue and nerve damage. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:52:15Z 2018-11-26T17:52:15Z 2018-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.micapath.2018.03.050 Microbial Pathogenesis. London: Academic Press Ltd- Elsevier Science Ltd, v. 118, p. 277-284, 2018. 0882-4010 http://hdl.handle.net/11449/164353 10.1016/j.micapath.2018.03.050 WOS:000436528000038 WOS000436528000038.pdf |
url |
http://dx.doi.org/10.1016/j.micapath.2018.03.050 http://hdl.handle.net/11449/164353 |
identifier_str_mv |
Microbial Pathogenesis. London: Academic Press Ltd- Elsevier Science Ltd, v. 118, p. 277-284, 2018. 0882-4010 10.1016/j.micapath.2018.03.050 WOS:000436528000038 WOS000436528000038.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microbial Pathogenesis 0,751 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
277-284 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129593309134848 |