Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions

Detalhes bibliográficos
Autor(a) principal: Costa, Mauricio B.
Data de Publicação: 2018
Outros Autores: Mimura, Kallyne K. O. [UNESP], Freitas, Aline A., Hungria, Emerith M., Sousa, Ana Lucia O. M., Oliani, Sonia M. [UNESP], Stefani, Mariane M. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.micapath.2018.03.050
http://hdl.handle.net/11449/164353
Resumo: Mast cells (MCs) have important immunoregulatory roles in skin inflammation. Annexin A1 (ANXA1) is an endogenous anti-inflammatory protein that can be expressed by mast cells, neutrophils, eosinophils, monocytes, epithelial and T cells. This study investigated MCs heterogeneity and ANXA1 expression in human dermatoses with special emphasis in leprosy. Sixty one skin biopsies from 2 groups were investigated: 40 newly diagnosed untreated leprosy patients (18 reaction-free, 11 type 1 reaction/T1R, 11 type 2 reaction/T2R); 21 patients with other dermatoses. Tryptase/try + and chymase/chy + phenotypic markers and toluidine blue stained intact/degranulated MC counts/mm(2) were evaluated. Try(+)/chy(+) MCs and ANXA1 were identified by streptavidin-biotin-peroxidase immunostaining and density was reported. In leprosy, degranulated MCs outnumbered intact ones regardless of the leprosy form (from tuberculoid/TT to lepromatous/LL), leprosy reactions (reactional/reaction-free) and type of reaction (T1R/T2R). Compared to other dermatoses, leprosy skin lesions showed lower numbers of degranulated and intact MCs. Try(+) MCs outnumbered chy(+) in leprosy lesions (reaction-free/reactional, particularly in T2R), but not in other dermatoses. Compared to other dermatoses, ANXA1 expression, which is also expressed in mast cells, was higher in the epidermis of leprosy skin lesions, independently of reactional episode. In leprosy, higher MC degranulation and differential expression of try(+)/chy(+) subsets independent of leprosy type and reaction suggest that the Mycobacterium leprae infection itself dictates the inflammatory MCs activation in skin lesions. Higher expression of ANXA1 in leprosy suggests its potential anti-inflammatory role to maintain homeostasis preventing tissue and nerve damage.
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spelling Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesionsMast cellAnnexin A1TryptaseChymaseLeprosySkin diseasesMast cells (MCs) have important immunoregulatory roles in skin inflammation. Annexin A1 (ANXA1) is an endogenous anti-inflammatory protein that can be expressed by mast cells, neutrophils, eosinophils, monocytes, epithelial and T cells. This study investigated MCs heterogeneity and ANXA1 expression in human dermatoses with special emphasis in leprosy. Sixty one skin biopsies from 2 groups were investigated: 40 newly diagnosed untreated leprosy patients (18 reaction-free, 11 type 1 reaction/T1R, 11 type 2 reaction/T2R); 21 patients with other dermatoses. Tryptase/try + and chymase/chy + phenotypic markers and toluidine blue stained intact/degranulated MC counts/mm(2) were evaluated. Try(+)/chy(+) MCs and ANXA1 were identified by streptavidin-biotin-peroxidase immunostaining and density was reported. In leprosy, degranulated MCs outnumbered intact ones regardless of the leprosy form (from tuberculoid/TT to lepromatous/LL), leprosy reactions (reactional/reaction-free) and type of reaction (T1R/T2R). Compared to other dermatoses, leprosy skin lesions showed lower numbers of degranulated and intact MCs. Try(+) MCs outnumbered chy(+) in leprosy lesions (reaction-free/reactional, particularly in T2R), but not in other dermatoses. Compared to other dermatoses, ANXA1 expression, which is also expressed in mast cells, was higher in the epidermis of leprosy skin lesions, independently of reactional episode. In leprosy, higher MC degranulation and differential expression of try(+)/chy(+) subsets independent of leprosy type and reaction suggest that the Mycobacterium leprae infection itself dictates the inflammatory MCs activation in skin lesions. Higher expression of ANXA1 in leprosy suggests its potential anti-inflammatory role to maintain homeostasis preventing tissue and nerve damage.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Goias, Trop Pathol & Publ Hlth Inst, Rua 235 S-N Setor Univ, BR-74605050 Goiania, Go, BrazilSao Paulo State Univ, UNESP, Dept Biol, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, SP, BrazilSao Paulo State Univ, UNESP, Dept Biol, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, SP, BrazilCNPq: 304869/2008-2CNPq: 308144/2014-7CNPq: 141554/2013-4FAPESP: 2012/21603-2FAPESP: 2012/13041-4CAPES: 1054292Elsevier B.V.Universidade Federal de Goiás (UFG)Universidade Estadual Paulista (Unesp)Costa, Mauricio B.Mimura, Kallyne K. O. [UNESP]Freitas, Aline A.Hungria, Emerith M.Sousa, Ana Lucia O. M.Oliani, Sonia M. [UNESP]Stefani, Mariane M. A.2018-11-26T17:52:15Z2018-11-26T17:52:15Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article277-284application/pdfhttp://dx.doi.org/10.1016/j.micapath.2018.03.050Microbial Pathogenesis. London: Academic Press Ltd- Elsevier Science Ltd, v. 118, p. 277-284, 2018.0882-4010http://hdl.handle.net/11449/16435310.1016/j.micapath.2018.03.050WOS:000436528000038WOS000436528000038.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Pathogenesis0,751info:eu-repo/semantics/openAccess2024-01-29T06:24:11Zoai:repositorio.unesp.br:11449/164353Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:11:28.471346Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
title Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
spellingShingle Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
Costa, Mauricio B.
Mast cell
Annexin A1
Tryptase
Chymase
Leprosy
Skin diseases
title_short Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
title_full Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
title_fullStr Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
title_full_unstemmed Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
title_sort Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
author Costa, Mauricio B.
author_facet Costa, Mauricio B.
Mimura, Kallyne K. O. [UNESP]
Freitas, Aline A.
Hungria, Emerith M.
Sousa, Ana Lucia O. M.
Oliani, Sonia M. [UNESP]
Stefani, Mariane M. A.
author_role author
author2 Mimura, Kallyne K. O. [UNESP]
Freitas, Aline A.
Hungria, Emerith M.
Sousa, Ana Lucia O. M.
Oliani, Sonia M. [UNESP]
Stefani, Mariane M. A.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Goiás (UFG)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Costa, Mauricio B.
Mimura, Kallyne K. O. [UNESP]
Freitas, Aline A.
Hungria, Emerith M.
Sousa, Ana Lucia O. M.
Oliani, Sonia M. [UNESP]
Stefani, Mariane M. A.
dc.subject.por.fl_str_mv Mast cell
Annexin A1
Tryptase
Chymase
Leprosy
Skin diseases
topic Mast cell
Annexin A1
Tryptase
Chymase
Leprosy
Skin diseases
description Mast cells (MCs) have important immunoregulatory roles in skin inflammation. Annexin A1 (ANXA1) is an endogenous anti-inflammatory protein that can be expressed by mast cells, neutrophils, eosinophils, monocytes, epithelial and T cells. This study investigated MCs heterogeneity and ANXA1 expression in human dermatoses with special emphasis in leprosy. Sixty one skin biopsies from 2 groups were investigated: 40 newly diagnosed untreated leprosy patients (18 reaction-free, 11 type 1 reaction/T1R, 11 type 2 reaction/T2R); 21 patients with other dermatoses. Tryptase/try + and chymase/chy + phenotypic markers and toluidine blue stained intact/degranulated MC counts/mm(2) were evaluated. Try(+)/chy(+) MCs and ANXA1 were identified by streptavidin-biotin-peroxidase immunostaining and density was reported. In leprosy, degranulated MCs outnumbered intact ones regardless of the leprosy form (from tuberculoid/TT to lepromatous/LL), leprosy reactions (reactional/reaction-free) and type of reaction (T1R/T2R). Compared to other dermatoses, leprosy skin lesions showed lower numbers of degranulated and intact MCs. Try(+) MCs outnumbered chy(+) in leprosy lesions (reaction-free/reactional, particularly in T2R), but not in other dermatoses. Compared to other dermatoses, ANXA1 expression, which is also expressed in mast cells, was higher in the epidermis of leprosy skin lesions, independently of reactional episode. In leprosy, higher MC degranulation and differential expression of try(+)/chy(+) subsets independent of leprosy type and reaction suggest that the Mycobacterium leprae infection itself dictates the inflammatory MCs activation in skin lesions. Higher expression of ANXA1 in leprosy suggests its potential anti-inflammatory role to maintain homeostasis preventing tissue and nerve damage.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-26T17:52:15Z
2018-11-26T17:52:15Z
2018-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.micapath.2018.03.050
Microbial Pathogenesis. London: Academic Press Ltd- Elsevier Science Ltd, v. 118, p. 277-284, 2018.
0882-4010
http://hdl.handle.net/11449/164353
10.1016/j.micapath.2018.03.050
WOS:000436528000038
WOS000436528000038.pdf
url http://dx.doi.org/10.1016/j.micapath.2018.03.050
http://hdl.handle.net/11449/164353
identifier_str_mv Microbial Pathogenesis. London: Academic Press Ltd- Elsevier Science Ltd, v. 118, p. 277-284, 2018.
0882-4010
10.1016/j.micapath.2018.03.050
WOS:000436528000038
WOS000436528000038.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microbial Pathogenesis
0,751
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 277-284
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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