Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.micpath.2018.03.050 http://hdl.handle.net/11449/176099 |
Resumo: | Mast cells (MCs) have important immunoregulatory roles in skin inflammation. Annexin A1 (ANXA1) is an endogenous anti-inflammatory protein that can be expressed by mast cells, neutrophils, eosinophils, monocytes, epithelial and T cells. This study investigated MCs heterogeneity and ANXA1 expression in human dermatoses with special emphasis in leprosy. Sixty one skin biopsies from 2 groups were investigated: 40 newly diagnosed untreated leprosy patients (18 reaction-free, 11 type 1 reaction/T1R, 11 type 2 reaction/T2R); 21 patients with other dermatoses. Tryptase/try+ and chymase/chy + phenotypic markers and toluidine blue stained intact/degranulated MC counts/mm2 were evaluated. Try+/chy+ MCs and ANXA1 were identified by streptavidin-biotin-peroxidase immunostaining and density was reported. In leprosy, degranulated MCs outnumbered intact ones regardless of the leprosy form (from tuberculoid/TT to lepromatous/LL), leprosy reactions (reactional/reaction-free) and type of reaction (T1R/T2R). Compared to other dermatoses, leprosy skin lesions showed lower numbers of degranulated and intact MCs. Try+ MCs outnumbered chy+ in leprosy lesions (reaction-free/reactional, particularly in T2R), but not in other dermatoses. Compared to other dermatoses, ANXA1 expression, which is also expressed in mast cells, was higher in the epidermis of leprosy skin lesions, independently of reactional episode. In leprosy, higher MC degranulation and differential expression of try+/chy+ subsets independent of leprosy type and reaction suggest that the Mycobacterium leprae infection itself dictates the inflammatory MCs activation in skin lesions. Higher expression of ANXA1 in leprosy suggests its potential anti-inflammatory role to maintain homeostasis preventing tissue and nerve damage. |
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Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesionsAnnexin A1ChymaseLeprosyMast cellSkin diseasesTryptaseMast cells (MCs) have important immunoregulatory roles in skin inflammation. Annexin A1 (ANXA1) is an endogenous anti-inflammatory protein that can be expressed by mast cells, neutrophils, eosinophils, monocytes, epithelial and T cells. This study investigated MCs heterogeneity and ANXA1 expression in human dermatoses with special emphasis in leprosy. Sixty one skin biopsies from 2 groups were investigated: 40 newly diagnosed untreated leprosy patients (18 reaction-free, 11 type 1 reaction/T1R, 11 type 2 reaction/T2R); 21 patients with other dermatoses. Tryptase/try+ and chymase/chy + phenotypic markers and toluidine blue stained intact/degranulated MC counts/mm2 were evaluated. Try+/chy+ MCs and ANXA1 were identified by streptavidin-biotin-peroxidase immunostaining and density was reported. In leprosy, degranulated MCs outnumbered intact ones regardless of the leprosy form (from tuberculoid/TT to lepromatous/LL), leprosy reactions (reactional/reaction-free) and type of reaction (T1R/T2R). Compared to other dermatoses, leprosy skin lesions showed lower numbers of degranulated and intact MCs. Try+ MCs outnumbered chy+ in leprosy lesions (reaction-free/reactional, particularly in T2R), but not in other dermatoses. Compared to other dermatoses, ANXA1 expression, which is also expressed in mast cells, was higher in the epidermis of leprosy skin lesions, independently of reactional episode. In leprosy, higher MC degranulation and differential expression of try+/chy+ subsets independent of leprosy type and reaction suggest that the Mycobacterium leprae infection itself dictates the inflammatory MCs activation in skin lesions. Higher expression of ANXA1 in leprosy suggests its potential anti-inflammatory role to maintain homeostasis preventing tissue and nerve damage.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Tropical Pathology and Public Health Institute Federal University of GoiásDepartment of Biology Instituto de Biociências Letras e Ciências Exatas Sao Paulo State University UNESPDepartment of Biology Instituto de Biociências Letras e Ciências Exatas Sao Paulo State University UNESPCAPES: #1054292Universidade Federal de Goiás (UFG)Universidade Estadual Paulista (Unesp)Costa, Maurício B.Mimura, Kallyne K.O. [UNESP]Freitas, Aline A.Hungria, Emerith M.Sousa, Ana Lúcia O.M.Oliani, Sonia M. [UNESP]Stefani, Mariane M.A.2018-12-11T17:19:03Z2018-12-11T17:19:03Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article277-284application/pdfhttp://dx.doi.org/10.1016/j.micpath.2018.03.050Microbial Pathogenesis, v. 118, p. 277-284.1096-12080882-4010http://hdl.handle.net/11449/17609910.1016/j.micpath.2018.03.0502-s2.0-850445899522-s2.0-85044589952.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Pathogenesis0,7510,751info:eu-repo/semantics/openAccess2024-01-11T06:30:22Zoai:repositorio.unesp.br:11449/176099Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:42:52.153729Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions |
title |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions |
spellingShingle |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions Costa, Maurício B. Annexin A1 Chymase Leprosy Mast cell Skin diseases Tryptase |
title_short |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions |
title_full |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions |
title_fullStr |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions |
title_full_unstemmed |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions |
title_sort |
Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions |
author |
Costa, Maurício B. |
author_facet |
Costa, Maurício B. Mimura, Kallyne K.O. [UNESP] Freitas, Aline A. Hungria, Emerith M. Sousa, Ana Lúcia O.M. Oliani, Sonia M. [UNESP] Stefani, Mariane M.A. |
author_role |
author |
author2 |
Mimura, Kallyne K.O. [UNESP] Freitas, Aline A. Hungria, Emerith M. Sousa, Ana Lúcia O.M. Oliani, Sonia M. [UNESP] Stefani, Mariane M.A. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Goiás (UFG) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Costa, Maurício B. Mimura, Kallyne K.O. [UNESP] Freitas, Aline A. Hungria, Emerith M. Sousa, Ana Lúcia O.M. Oliani, Sonia M. [UNESP] Stefani, Mariane M.A. |
dc.subject.por.fl_str_mv |
Annexin A1 Chymase Leprosy Mast cell Skin diseases Tryptase |
topic |
Annexin A1 Chymase Leprosy Mast cell Skin diseases Tryptase |
description |
Mast cells (MCs) have important immunoregulatory roles in skin inflammation. Annexin A1 (ANXA1) is an endogenous anti-inflammatory protein that can be expressed by mast cells, neutrophils, eosinophils, monocytes, epithelial and T cells. This study investigated MCs heterogeneity and ANXA1 expression in human dermatoses with special emphasis in leprosy. Sixty one skin biopsies from 2 groups were investigated: 40 newly diagnosed untreated leprosy patients (18 reaction-free, 11 type 1 reaction/T1R, 11 type 2 reaction/T2R); 21 patients with other dermatoses. Tryptase/try+ and chymase/chy + phenotypic markers and toluidine blue stained intact/degranulated MC counts/mm2 were evaluated. Try+/chy+ MCs and ANXA1 were identified by streptavidin-biotin-peroxidase immunostaining and density was reported. In leprosy, degranulated MCs outnumbered intact ones regardless of the leprosy form (from tuberculoid/TT to lepromatous/LL), leprosy reactions (reactional/reaction-free) and type of reaction (T1R/T2R). Compared to other dermatoses, leprosy skin lesions showed lower numbers of degranulated and intact MCs. Try+ MCs outnumbered chy+ in leprosy lesions (reaction-free/reactional, particularly in T2R), but not in other dermatoses. Compared to other dermatoses, ANXA1 expression, which is also expressed in mast cells, was higher in the epidermis of leprosy skin lesions, independently of reactional episode. In leprosy, higher MC degranulation and differential expression of try+/chy+ subsets independent of leprosy type and reaction suggest that the Mycobacterium leprae infection itself dictates the inflammatory MCs activation in skin lesions. Higher expression of ANXA1 in leprosy suggests its potential anti-inflammatory role to maintain homeostasis preventing tissue and nerve damage. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:19:03Z 2018-12-11T17:19:03Z 2018-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.micpath.2018.03.050 Microbial Pathogenesis, v. 118, p. 277-284. 1096-1208 0882-4010 http://hdl.handle.net/11449/176099 10.1016/j.micpath.2018.03.050 2-s2.0-85044589952 2-s2.0-85044589952.pdf |
url |
http://dx.doi.org/10.1016/j.micpath.2018.03.050 http://hdl.handle.net/11449/176099 |
identifier_str_mv |
Microbial Pathogenesis, v. 118, p. 277-284. 1096-1208 0882-4010 10.1016/j.micpath.2018.03.050 2-s2.0-85044589952 2-s2.0-85044589952.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microbial Pathogenesis 0,751 0,751 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
277-284 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129453346258944 |