Immunosensor for diagnosis of Alzheimer disease using amyloid-β 1-40 peptide and silk fibroin thin films
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.1016/j.msec.2016.05.101 http://hdl.handle.net/11449/168712 |
Resumo: | Layer-by-Layer (LbL) films containing silk fibroin (SF) and the 40 aminoacid-long amyloid-β peptide (Aβ1-40) were prepared with the purpose of developing a new prototype of an electrochemical immunosensor. The film showed a satisfactory growth in quartz substrate and screen-printed carbon electrodes, as observed by UV-vis spectroscopy and cyclic voltammetric, respectively. The peptide immobilized in LbL films in junction with SF shows secondary structure induced, as shown by circular dichroism measurements, favoring the interaction SF/peptide LbL film with the specific antibody. Immunosensor showed a linear response in the presence of the antibody with concentrations from 0 to 10 ng mL- 1 both analyzed by current changes in 0.3 V and voltammogram area. This system can be applied as a new prototype for preliminary diagnosis of Alzheimer's disease. |
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Immunosensor for diagnosis of Alzheimer disease using amyloid-β 1-40 peptide and silk fibroin thin filmsAlzheimer's diseaseAmyloid-β peptideImmunosensorLayer-by-layerSilk fibroinLayer-by-Layer (LbL) films containing silk fibroin (SF) and the 40 aminoacid-long amyloid-β peptide (Aβ1-40) were prepared with the purpose of developing a new prototype of an electrochemical immunosensor. The film showed a satisfactory growth in quartz substrate and screen-printed carbon electrodes, as observed by UV-vis spectroscopy and cyclic voltammetric, respectively. The peptide immobilized in LbL films in junction with SF shows secondary structure induced, as shown by circular dichroism measurements, favoring the interaction SF/peptide LbL film with the specific antibody. Immunosensor showed a linear response in the presence of the antibody with concentrations from 0 to 10 ng mL- 1 both analyzed by current changes in 0.3 V and voltammogram area. This system can be applied as a new prototype for preliminary diagnosis of Alzheimer's disease.Instituto de Química Universidade Estadual Paulista Júlio de Mesquita Filho UNESP, Rua Professor Francisco Degni, 55Instituto de Ciência e Tecnologia Universidade Federal de São Paulo UNIFESP, Rua Talim, 330Instituto de Química Universidade Estadual Paulista Júlio de Mesquita Filho UNESP, Rua Professor Francisco Degni, 55Universidade Estadual Paulista (Unesp)Universidade Federal de São Paulo (UNIFESP)Gonçalves, J. M. [UNESP]Lima, L. R. [UNESP]Moraes, M. L.Ribeiro, S. J.L. [UNESP]2018-12-11T16:42:40Z2018-12-11T16:42:40Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article338-342application/pdfhttp://dx.doi.org/10.1016/j.msec.2016.05.101Materials Science and Engineering C, v. 68, p. 338-342.0928-4931http://hdl.handle.net/11449/16871210.1016/j.msec.2016.05.1012-s2.0-849732778312-s2.0-84973277831.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Science and Engineering C1,110info:eu-repo/semantics/openAccess2023-11-06T06:06:51Zoai:repositorio.unesp.br:11449/168712Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:00:13.218048Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Immunosensor for diagnosis of Alzheimer disease using amyloid-β 1-40 peptide and silk fibroin thin films |
| title |
Immunosensor for diagnosis of Alzheimer disease using amyloid-β 1-40 peptide and silk fibroin thin films |
| spellingShingle |
Immunosensor for diagnosis of Alzheimer disease using amyloid-β 1-40 peptide and silk fibroin thin films Gonçalves, J. M. [UNESP] Alzheimer's disease Amyloid-β peptide Immunosensor Layer-by-layer Silk fibroin |
| title_short |
Immunosensor for diagnosis of Alzheimer disease using amyloid-β 1-40 peptide and silk fibroin thin films |
| title_full |
Immunosensor for diagnosis of Alzheimer disease using amyloid-β 1-40 peptide and silk fibroin thin films |
| title_fullStr |
Immunosensor for diagnosis of Alzheimer disease using amyloid-β 1-40 peptide and silk fibroin thin films |
| title_full_unstemmed |
Immunosensor for diagnosis of Alzheimer disease using amyloid-β 1-40 peptide and silk fibroin thin films |
| title_sort |
Immunosensor for diagnosis of Alzheimer disease using amyloid-β 1-40 peptide and silk fibroin thin films |
| author |
Gonçalves, J. M. [UNESP] |
| author_facet |
Gonçalves, J. M. [UNESP] Lima, L. R. [UNESP] Moraes, M. L. Ribeiro, S. J.L. [UNESP] |
| author_role |
author |
| author2 |
Lima, L. R. [UNESP] Moraes, M. L. Ribeiro, S. J.L. [UNESP] |
| author2_role |
author author author |
| dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Paulo (UNIFESP) |
| dc.contributor.author.fl_str_mv |
Gonçalves, J. M. [UNESP] Lima, L. R. [UNESP] Moraes, M. L. Ribeiro, S. J.L. [UNESP] |
| dc.subject.por.fl_str_mv |
Alzheimer's disease Amyloid-β peptide Immunosensor Layer-by-layer Silk fibroin |
| topic |
Alzheimer's disease Amyloid-β peptide Immunosensor Layer-by-layer Silk fibroin |
| description |
Layer-by-Layer (LbL) films containing silk fibroin (SF) and the 40 aminoacid-long amyloid-β peptide (Aβ1-40) were prepared with the purpose of developing a new prototype of an electrochemical immunosensor. The film showed a satisfactory growth in quartz substrate and screen-printed carbon electrodes, as observed by UV-vis spectroscopy and cyclic voltammetric, respectively. The peptide immobilized in LbL films in junction with SF shows secondary structure induced, as shown by circular dichroism measurements, favoring the interaction SF/peptide LbL film with the specific antibody. Immunosensor showed a linear response in the presence of the antibody with concentrations from 0 to 10 ng mL- 1 both analyzed by current changes in 0.3 V and voltammogram area. This system can be applied as a new prototype for preliminary diagnosis of Alzheimer's disease. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016-11-01 2018-12-11T16:42:40Z 2018-12-11T16:42:40Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.msec.2016.05.101 Materials Science and Engineering C, v. 68, p. 338-342. 0928-4931 http://hdl.handle.net/11449/168712 10.1016/j.msec.2016.05.101 2-s2.0-84973277831 2-s2.0-84973277831.pdf |
| url |
http://dx.doi.org/10.1016/j.msec.2016.05.101 http://hdl.handle.net/11449/168712 |
| identifier_str_mv |
Materials Science and Engineering C, v. 68, p. 338-342. 0928-4931 10.1016/j.msec.2016.05.101 2-s2.0-84973277831 2-s2.0-84973277831.pdf |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Materials Science and Engineering C 1,110 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
338-342 application/pdf |
| dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
| instname_str |
Universidade Estadual Paulista (UNESP) |
| instacron_str |
UNESP |
| institution |
UNESP |
| reponame_str |
Repositório Institucional da UNESP |
| collection |
Repositório Institucional da UNESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
| repository.mail.fl_str_mv |
|
| _version_ |
1808128735702941696 |