Protective effects of piperlongumin in the prevention of inflammatory damage caused by pulmonary exposure to benzopyrene carcinogen
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.intimp.2021.108285 http://hdl.handle.net/11449/233816 |
Resumo: | Benzopyrene is one of the main polycyclic aromatic hydrocarbons with carcinogenic capacity. Research has shown that anti-inflammatory drugs can reduce the incidence of lung cancer. In this scenario, we highlight piperlongumin (PL), an alkaloid from Piper longum with anti-inflammatory properties. Therefore, our aim was to study the effect of PL administration in a model of pulmonary carcinogenesis induced by benzopyrene in Balb/c mice. Animals were divided into 3 groups (n = 10/group): sham (10% DMSO), induced by benzopyrene (100 mg/kg, diluted in DMSO) without treatment (BaP) for 12 weeks and induced by benzopyrene and treated with PL (BaP/PL) (2 mg/kg in 10% DMSO) from the eighth week post-induction. Animals were weighed daily and pletsmography was performed in the 12th week. Genotoxicity and hemoglobin levels were analyzed in blood and quantification of leukocytes in bronchoalveolar lavage (BAL). Lungs were collected for histopathological evaluation, immunohistochemical studies of annexin A1 (AnxA1), cyclooxygenase 2 (COX-2), anti-apoptotic protein Bcl-2 and nuclear transcription factor (NF-kB) and also the measurement of interleukin cytokines (IL)-1β, IL-17 and tumor necrosis factor (TNF) -α. Treatment with PL reduced the pulmonary parameters (p < 0,001) of frequency, volume and pulmonary ventilation, decreased lymphocytes, monocytes and neutrophils in BAL (p < 0,05) as well as blood hemoglobin levels (p < 0,01). PL administration also reduced DNA damage and preserved the pulmonary architecture compared to the BaP group. Moreover, the anti-inflammatory effect of PL was evidenced by the maintenance of AnxA1 levels, reduction of COX-2 (p < 0,05), Bcl-2 (p < 0,01) and NF-kB (p < 0,001) expressions and decreased IL-1β, IL-17 (p < 0,01) and TNF-α (p < 0,05) levels. The results show the therapeutic potential of PL in the treatment of pulmonary anti-inflammatory and anti-tumor diseases with promising therapeutic implications. |
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Protective effects of piperlongumin in the prevention of inflammatory damage caused by pulmonary exposure to benzopyrene carcinogenAnxA1CytokinesLung cancerNatural bioactive compoundSmokingBenzopyrene is one of the main polycyclic aromatic hydrocarbons with carcinogenic capacity. Research has shown that anti-inflammatory drugs can reduce the incidence of lung cancer. In this scenario, we highlight piperlongumin (PL), an alkaloid from Piper longum with anti-inflammatory properties. Therefore, our aim was to study the effect of PL administration in a model of pulmonary carcinogenesis induced by benzopyrene in Balb/c mice. Animals were divided into 3 groups (n = 10/group): sham (10% DMSO), induced by benzopyrene (100 mg/kg, diluted in DMSO) without treatment (BaP) for 12 weeks and induced by benzopyrene and treated with PL (BaP/PL) (2 mg/kg in 10% DMSO) from the eighth week post-induction. Animals were weighed daily and pletsmography was performed in the 12th week. Genotoxicity and hemoglobin levels were analyzed in blood and quantification of leukocytes in bronchoalveolar lavage (BAL). Lungs were collected for histopathological evaluation, immunohistochemical studies of annexin A1 (AnxA1), cyclooxygenase 2 (COX-2), anti-apoptotic protein Bcl-2 and nuclear transcription factor (NF-kB) and also the measurement of interleukin cytokines (IL)-1β, IL-17 and tumor necrosis factor (TNF) -α. Treatment with PL reduced the pulmonary parameters (p < 0,001) of frequency, volume and pulmonary ventilation, decreased lymphocytes, monocytes and neutrophils in BAL (p < 0,05) as well as blood hemoglobin levels (p < 0,01). PL administration also reduced DNA damage and preserved the pulmonary architecture compared to the BaP group. Moreover, the anti-inflammatory effect of PL was evidenced by the maintenance of AnxA1 levels, reduction of COX-2 (p < 0,05), Bcl-2 (p < 0,01) and NF-kB (p < 0,001) expressions and decreased IL-1β, IL-17 (p < 0,01) and TNF-α (p < 0,05) levels. The results show the therapeutic potential of PL in the treatment of pulmonary anti-inflammatory and anti-tumor diseases with promising therapeutic implications.São Paulo State University (UNESP) Department of Biology Laboratory of Immunomorphology, Cristovão Colombo Street, 2265University Center Padre Albino (UNIFIPA), Estudantes Street, 225Federal University of São Paulo (UNIFESP) Graduate Program in Structural and Functional Biology, Sena Madureira Street, 1500São José do Rio Preto School of Medicine (FAMERP), Brigadeiro Faria Lima Avenue, 5416Federal University of São Paulo Department of Gynecology Escola Paulista de Medicina (UNIFESP-EPM), Sena Madureira Street, 1500São Paulo State University (UNESP) Department of Biology Laboratory of Immunomorphology, Cristovão Colombo Street, 2265Universidade Estadual Paulista (UNESP)University Center Padre Albino (UNIFIPA)Universidade de São Paulo (USP)São José do Rio Preto School of Medicine (FAMERP)Ashino, Tissiane Eid Barbosa [UNESP]Sant́ Ana, Monielle LealYoshikawa, Ariane HarumiPossebon, Lucas [UNESP]de Souza Costa, Sara [UNESP]Iyomasa-Pilon, Melina MizusakiSouza, Helena Ribeiro [UNESP]Gonçalves, Giovana AparecidaOliani, Sonia Maria [UNESP]Girol, Ana Paula [UNESP]2022-05-01T10:35:01Z2022-05-01T10:35:01Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.intimp.2021.108285International Immunopharmacology.1878-17051567-5769http://hdl.handle.net/11449/23381610.1016/j.intimp.2021.1082852-s2.0-85119401734Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Immunopharmacologyinfo:eu-repo/semantics/openAccess2022-05-01T10:35:01Zoai:repositorio.unesp.br:11449/233816Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:12:08.371115Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Protective effects of piperlongumin in the prevention of inflammatory damage caused by pulmonary exposure to benzopyrene carcinogen |
title |
Protective effects of piperlongumin in the prevention of inflammatory damage caused by pulmonary exposure to benzopyrene carcinogen |
spellingShingle |
Protective effects of piperlongumin in the prevention of inflammatory damage caused by pulmonary exposure to benzopyrene carcinogen Ashino, Tissiane Eid Barbosa [UNESP] AnxA1 Cytokines Lung cancer Natural bioactive compound Smoking |
title_short |
Protective effects of piperlongumin in the prevention of inflammatory damage caused by pulmonary exposure to benzopyrene carcinogen |
title_full |
Protective effects of piperlongumin in the prevention of inflammatory damage caused by pulmonary exposure to benzopyrene carcinogen |
title_fullStr |
Protective effects of piperlongumin in the prevention of inflammatory damage caused by pulmonary exposure to benzopyrene carcinogen |
title_full_unstemmed |
Protective effects of piperlongumin in the prevention of inflammatory damage caused by pulmonary exposure to benzopyrene carcinogen |
title_sort |
Protective effects of piperlongumin in the prevention of inflammatory damage caused by pulmonary exposure to benzopyrene carcinogen |
author |
Ashino, Tissiane Eid Barbosa [UNESP] |
author_facet |
Ashino, Tissiane Eid Barbosa [UNESP] Sant́ Ana, Monielle Leal Yoshikawa, Ariane Harumi Possebon, Lucas [UNESP] de Souza Costa, Sara [UNESP] Iyomasa-Pilon, Melina Mizusaki Souza, Helena Ribeiro [UNESP] Gonçalves, Giovana Aparecida Oliani, Sonia Maria [UNESP] Girol, Ana Paula [UNESP] |
author_role |
author |
author2 |
Sant́ Ana, Monielle Leal Yoshikawa, Ariane Harumi Possebon, Lucas [UNESP] de Souza Costa, Sara [UNESP] Iyomasa-Pilon, Melina Mizusaki Souza, Helena Ribeiro [UNESP] Gonçalves, Giovana Aparecida Oliani, Sonia Maria [UNESP] Girol, Ana Paula [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University Center Padre Albino (UNIFIPA) Universidade de São Paulo (USP) São José do Rio Preto School of Medicine (FAMERP) |
dc.contributor.author.fl_str_mv |
Ashino, Tissiane Eid Barbosa [UNESP] Sant́ Ana, Monielle Leal Yoshikawa, Ariane Harumi Possebon, Lucas [UNESP] de Souza Costa, Sara [UNESP] Iyomasa-Pilon, Melina Mizusaki Souza, Helena Ribeiro [UNESP] Gonçalves, Giovana Aparecida Oliani, Sonia Maria [UNESP] Girol, Ana Paula [UNESP] |
dc.subject.por.fl_str_mv |
AnxA1 Cytokines Lung cancer Natural bioactive compound Smoking |
topic |
AnxA1 Cytokines Lung cancer Natural bioactive compound Smoking |
description |
Benzopyrene is one of the main polycyclic aromatic hydrocarbons with carcinogenic capacity. Research has shown that anti-inflammatory drugs can reduce the incidence of lung cancer. In this scenario, we highlight piperlongumin (PL), an alkaloid from Piper longum with anti-inflammatory properties. Therefore, our aim was to study the effect of PL administration in a model of pulmonary carcinogenesis induced by benzopyrene in Balb/c mice. Animals were divided into 3 groups (n = 10/group): sham (10% DMSO), induced by benzopyrene (100 mg/kg, diluted in DMSO) without treatment (BaP) for 12 weeks and induced by benzopyrene and treated with PL (BaP/PL) (2 mg/kg in 10% DMSO) from the eighth week post-induction. Animals were weighed daily and pletsmography was performed in the 12th week. Genotoxicity and hemoglobin levels were analyzed in blood and quantification of leukocytes in bronchoalveolar lavage (BAL). Lungs were collected for histopathological evaluation, immunohistochemical studies of annexin A1 (AnxA1), cyclooxygenase 2 (COX-2), anti-apoptotic protein Bcl-2 and nuclear transcription factor (NF-kB) and also the measurement of interleukin cytokines (IL)-1β, IL-17 and tumor necrosis factor (TNF) -α. Treatment with PL reduced the pulmonary parameters (p < 0,001) of frequency, volume and pulmonary ventilation, decreased lymphocytes, monocytes and neutrophils in BAL (p < 0,05) as well as blood hemoglobin levels (p < 0,01). PL administration also reduced DNA damage and preserved the pulmonary architecture compared to the BaP group. Moreover, the anti-inflammatory effect of PL was evidenced by the maintenance of AnxA1 levels, reduction of COX-2 (p < 0,05), Bcl-2 (p < 0,01) and NF-kB (p < 0,001) expressions and decreased IL-1β, IL-17 (p < 0,01) and TNF-α (p < 0,05) levels. The results show the therapeutic potential of PL in the treatment of pulmonary anti-inflammatory and anti-tumor diseases with promising therapeutic implications. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-05-01T10:35:01Z 2022-05-01T10:35:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.intimp.2021.108285 International Immunopharmacology. 1878-1705 1567-5769 http://hdl.handle.net/11449/233816 10.1016/j.intimp.2021.108285 2-s2.0-85119401734 |
url |
http://dx.doi.org/10.1016/j.intimp.2021.108285 http://hdl.handle.net/11449/233816 |
identifier_str_mv |
International Immunopharmacology. 1878-1705 1567-5769 10.1016/j.intimp.2021.108285 2-s2.0-85119401734 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Immunopharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128770318532608 |