Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy

Detalhes bibliográficos
Autor(a) principal: Abreu, Mariana Gouvêa Latini
Data de Publicação: 2019
Outros Autores: Kawamoto, Dione, Mayer, Marcia Pinto Alves, Pascoal, Vinicius D’Avila Bitencourt, Caiaffa, Karina Sampaio [UNESP], Zuza, Elizangela P., Duque, Cristiane [UNESP], Camargo, Gabriela Alessandra da Cruz Galhardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1678-7757-2018-0205
http://hdl.handle.net/11449/189047
Resumo: Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives: The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods: Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results: Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion: The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA.
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spelling Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapyPeriodontal diseasePorphyromonasSmokingPorphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives: The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods: Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results: Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion: The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal Fluminense Departamento de Odontologia Área de PeriodontiaUniversidade de São Paulo Instituto de Ciências Biomédicas Departamento de MicrobiologiaUniversidade Federal Fluminense Departamento de Ciências BásicasUniversidade Estadual Paulista (UNESP) Faculdade de Odontologia de Araçatuba Departamento de Endodontia AraçatubaUniversidade Estadual Paulista (UNESP) Faculdade de Odontologia de Araçatuba Departamento de Odontopediatria e Saúde PúblicaUniversidade Estadual Paulista (UNESP) Faculdade de Odontologia de Araçatuba Departamento de Endodontia AraçatubaUniversidade Estadual Paulista (UNESP) Faculdade de Odontologia de Araçatuba Departamento de Odontopediatria e Saúde PúblicaUniversidade Federal Fluminense (UFF)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Abreu, Mariana Gouvêa LatiniKawamoto, DioneMayer, Marcia Pinto AlvesPascoal, Vinicius D’Avila BitencourtCaiaffa, Karina Sampaio [UNESP]Zuza, Elizangela P.Duque, Cristiane [UNESP]Camargo, Gabriela Alessandra da Cruz Galhardo2019-10-06T16:28:05Z2019-10-06T16:28:05Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/1678-7757-2018-0205Journal of Applied Oral Science, v. 27.1678-77651678-7757http://hdl.handle.net/11449/18904710.1590/1678-7757-2018-0205S1678-775720190001004412-s2.0-85064975217S1678-77572019000100441.pdf56518745094936170000-0002-2575-279XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Scienceinfo:eu-repo/semantics/openAccess2024-09-19T18:31:51Zoai:repositorio.unesp.br:11449/189047Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:31:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
title Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
spellingShingle Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
Abreu, Mariana Gouvêa Latini
Periodontal disease
Porphyromonas
Smoking
title_short Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
title_full Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
title_fullStr Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
title_full_unstemmed Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
title_sort Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
author Abreu, Mariana Gouvêa Latini
author_facet Abreu, Mariana Gouvêa Latini
Kawamoto, Dione
Mayer, Marcia Pinto Alves
Pascoal, Vinicius D’Avila Bitencourt
Caiaffa, Karina Sampaio [UNESP]
Zuza, Elizangela P.
Duque, Cristiane [UNESP]
Camargo, Gabriela Alessandra da Cruz Galhardo
author_role author
author2 Kawamoto, Dione
Mayer, Marcia Pinto Alves
Pascoal, Vinicius D’Avila Bitencourt
Caiaffa, Karina Sampaio [UNESP]
Zuza, Elizangela P.
Duque, Cristiane [UNESP]
Camargo, Gabriela Alessandra da Cruz Galhardo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal Fluminense (UFF)
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Abreu, Mariana Gouvêa Latini
Kawamoto, Dione
Mayer, Marcia Pinto Alves
Pascoal, Vinicius D’Avila Bitencourt
Caiaffa, Karina Sampaio [UNESP]
Zuza, Elizangela P.
Duque, Cristiane [UNESP]
Camargo, Gabriela Alessandra da Cruz Galhardo
dc.subject.por.fl_str_mv Periodontal disease
Porphyromonas
Smoking
topic Periodontal disease
Porphyromonas
Smoking
description Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives: The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods: Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results: Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion: The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:28:05Z
2019-10-06T16:28:05Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1678-7757-2018-0205
Journal of Applied Oral Science, v. 27.
1678-7765
1678-7757
http://hdl.handle.net/11449/189047
10.1590/1678-7757-2018-0205
S1678-77572019000100441
2-s2.0-85064975217
S1678-77572019000100441.pdf
5651874509493617
0000-0002-2575-279X
url http://dx.doi.org/10.1590/1678-7757-2018-0205
http://hdl.handle.net/11449/189047
identifier_str_mv Journal of Applied Oral Science, v. 27.
1678-7765
1678-7757
10.1590/1678-7757-2018-0205
S1678-77572019000100441
2-s2.0-85064975217
S1678-77572019000100441.pdf
5651874509493617
0000-0002-2575-279X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Applied Oral Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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