Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100441 |
Resumo: | Abstract Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA. |
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Journal of applied oral science (Online) |
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Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapyPeriodontal diseaseSmokingPorphyromonas gingivalisAbstract Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA.Faculdade De Odontologia De Bauru - USP2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100441Journal of Applied Oral Science v.27 2019reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/1678-7757-2018-0205info:eu-repo/semantics/openAccessABREU,Mariana Gouvêa LatiniKAWAMOTO,DioneMAYER,Marcia Pinto AlvesPASCOAL,Vinicius D’Avila BitencourtCAIAFFA,Karina SampaioZUZA,Elizangela P.DUQUE,CristianeCAMARGO,Gabriela Alessandra da Cruz Galhardoeng2019-04-24T00:00:00Zoai:scielo:S1678-77572019000100441Revistahttp://www.scielo.br/jaosPUBhttps://old.scielo.br/oai/scielo-oai.php||jaos@usp.br1678-77651678-7757opendoar:2019-04-24T00:00Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
title |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
spellingShingle |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy ABREU,Mariana Gouvêa Latini Periodontal disease Smoking Porphyromonas gingivalis |
title_short |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
title_full |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
title_fullStr |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
title_full_unstemmed |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
title_sort |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
author |
ABREU,Mariana Gouvêa Latini |
author_facet |
ABREU,Mariana Gouvêa Latini KAWAMOTO,Dione MAYER,Marcia Pinto Alves PASCOAL,Vinicius D’Avila Bitencourt CAIAFFA,Karina Sampaio ZUZA,Elizangela P. DUQUE,Cristiane CAMARGO,Gabriela Alessandra da Cruz Galhardo |
author_role |
author |
author2 |
KAWAMOTO,Dione MAYER,Marcia Pinto Alves PASCOAL,Vinicius D’Avila Bitencourt CAIAFFA,Karina Sampaio ZUZA,Elizangela P. DUQUE,Cristiane CAMARGO,Gabriela Alessandra da Cruz Galhardo |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
ABREU,Mariana Gouvêa Latini KAWAMOTO,Dione MAYER,Marcia Pinto Alves PASCOAL,Vinicius D’Avila Bitencourt CAIAFFA,Karina Sampaio ZUZA,Elizangela P. DUQUE,Cristiane CAMARGO,Gabriela Alessandra da Cruz Galhardo |
dc.subject.por.fl_str_mv |
Periodontal disease Smoking Porphyromonas gingivalis |
topic |
Periodontal disease Smoking Porphyromonas gingivalis |
description |
Abstract Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100441 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100441 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-7757-2018-0205 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
publisher.none.fl_str_mv |
Faculdade De Odontologia De Bauru - USP |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science v.27 2019 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1748936440067129344 |