Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of applied oral science (Online) |
Texto Completo: | https://www.revistas.usp.br/jaos/article/view/158662 |
Resumo: | Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives: The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods: Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results: Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion: The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA. |
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Journal of applied oral science (Online) |
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Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapyPeriodontal diseaseSmokingPorphyromonas gingivalisPorphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives: The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods: Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results: Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion: The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA.Universidade de São Paulo. Faculdade de Odontologia de Bauru2019-06-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/15866210.1590/1678-7757-2018-0205Journal of Applied Oral Science; Vol. 27 (2019); e20180205Journal of Applied Oral Science; Vol. 27 (2019); e20180205Journal of Applied Oral Science; v. 27 (2019); e201802051678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/158662/153679Copyright (c) 2019 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessAbreu, Mariana Gouvêa LatiniKawamoto, DioneMayer, Marcia Pinto AlvesPascoal, Vinicius D’Avila BitencourtCaiaffa, Karina SampaioZuza, Elizangela P.Duque, CristianeCamargo, Gabriela Alessandra da Cruz Galhardo2019-06-06T16:06:27Zoai:revistas.usp.br:article/158662Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2019-06-06T16:06:27Journal of applied oral science (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
title |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
spellingShingle |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy Abreu, Mariana Gouvêa Latini Periodontal disease Smoking Porphyromonas gingivalis |
title_short |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
title_full |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
title_fullStr |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
title_full_unstemmed |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
title_sort |
Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy |
author |
Abreu, Mariana Gouvêa Latini |
author_facet |
Abreu, Mariana Gouvêa Latini Kawamoto, Dione Mayer, Marcia Pinto Alves Pascoal, Vinicius D’Avila Bitencourt Caiaffa, Karina Sampaio Zuza, Elizangela P. Duque, Cristiane Camargo, Gabriela Alessandra da Cruz Galhardo |
author_role |
author |
author2 |
Kawamoto, Dione Mayer, Marcia Pinto Alves Pascoal, Vinicius D’Avila Bitencourt Caiaffa, Karina Sampaio Zuza, Elizangela P. Duque, Cristiane Camargo, Gabriela Alessandra da Cruz Galhardo |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Abreu, Mariana Gouvêa Latini Kawamoto, Dione Mayer, Marcia Pinto Alves Pascoal, Vinicius D’Avila Bitencourt Caiaffa, Karina Sampaio Zuza, Elizangela P. Duque, Cristiane Camargo, Gabriela Alessandra da Cruz Galhardo |
dc.subject.por.fl_str_mv |
Periodontal disease Smoking Porphyromonas gingivalis |
topic |
Periodontal disease Smoking Porphyromonas gingivalis |
description |
Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives: The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods: Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results: Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion: The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-06-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/158662 10.1590/1678-7757-2018-0205 |
url |
https://www.revistas.usp.br/jaos/article/view/158662 |
identifier_str_mv |
10.1590/1678-7757-2018-0205 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/jaos/article/view/158662/153679 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Journal of Applied Oral Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Journal of Applied Oral Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Odontologia de Bauru |
dc.source.none.fl_str_mv |
Journal of Applied Oral Science; Vol. 27 (2019); e20180205 Journal of Applied Oral Science; Vol. 27 (2019); e20180205 Journal of Applied Oral Science; v. 27 (2019); e20180205 1678-7765 1678-7757 reponame:Journal of applied oral science (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Journal of applied oral science (Online) |
collection |
Journal of applied oral science (Online) |
repository.name.fl_str_mv |
Journal of applied oral science (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||jaos@usp.br |
_version_ |
1800221681185193984 |