Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy

Detalhes bibliográficos
Autor(a) principal: Abreu, Mariana Gouvêa Latini
Data de Publicação: 2019
Outros Autores: Kawamoto, Dione, Mayer, Marcia Pinto Alves, Pascoal, Vinicius D’Avila Bitencourt, Caiaffa, Karina Sampaio, Zuza, Elizangela P., Duque, Cristiane, Camargo, Gabriela Alessandra da Cruz Galhardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of applied oral science (Online)
Texto Completo: https://www.revistas.usp.br/jaos/article/view/158662
Resumo: Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives: The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods: Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results: Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion: The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA.
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spelling Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapyPeriodontal diseaseSmokingPorphyromonas gingivalisPorphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives: The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods: Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results: Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion: The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA.Universidade de São Paulo. Faculdade de Odontologia de Bauru2019-06-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/jaos/article/view/15866210.1590/1678-7757-2018-0205Journal of Applied Oral Science; Vol. 27 (2019); e20180205Journal of Applied Oral Science; Vol. 27 (2019); e20180205Journal of Applied Oral Science; v. 27 (2019); e201802051678-77651678-7757reponame:Journal of applied oral science (Online)instname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/jaos/article/view/158662/153679Copyright (c) 2019 Journal of Applied Oral Scienceinfo:eu-repo/semantics/openAccessAbreu, Mariana Gouvêa LatiniKawamoto, DioneMayer, Marcia Pinto AlvesPascoal, Vinicius D’Avila BitencourtCaiaffa, Karina SampaioZuza, Elizangela P.Duque, CristianeCamargo, Gabriela Alessandra da Cruz Galhardo2019-06-06T16:06:27Zoai:revistas.usp.br:article/158662Revistahttp://www.scielo.br/jaosPUBhttps://www.revistas.usp.br/jaos/oai||jaos@usp.br1678-77651678-7757opendoar:2019-06-06T16:06:27Journal of applied oral science (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
title Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
spellingShingle Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
Abreu, Mariana Gouvêa Latini
Periodontal disease
Smoking
Porphyromonas gingivalis
title_short Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
title_full Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
title_fullStr Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
title_full_unstemmed Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
title_sort Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy
author Abreu, Mariana Gouvêa Latini
author_facet Abreu, Mariana Gouvêa Latini
Kawamoto, Dione
Mayer, Marcia Pinto Alves
Pascoal, Vinicius D’Avila Bitencourt
Caiaffa, Karina Sampaio
Zuza, Elizangela P.
Duque, Cristiane
Camargo, Gabriela Alessandra da Cruz Galhardo
author_role author
author2 Kawamoto, Dione
Mayer, Marcia Pinto Alves
Pascoal, Vinicius D’Avila Bitencourt
Caiaffa, Karina Sampaio
Zuza, Elizangela P.
Duque, Cristiane
Camargo, Gabriela Alessandra da Cruz Galhardo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Abreu, Mariana Gouvêa Latini
Kawamoto, Dione
Mayer, Marcia Pinto Alves
Pascoal, Vinicius D’Avila Bitencourt
Caiaffa, Karina Sampaio
Zuza, Elizangela P.
Duque, Cristiane
Camargo, Gabriela Alessandra da Cruz Galhardo
dc.subject.por.fl_str_mv Periodontal disease
Smoking
Porphyromonas gingivalis
topic Periodontal disease
Smoking
Porphyromonas gingivalis
description Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives: The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods: Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results: Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion: The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/jaos/article/view/158662
10.1590/1678-7757-2018-0205
url https://www.revistas.usp.br/jaos/article/view/158662
identifier_str_mv 10.1590/1678-7757-2018-0205
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/jaos/article/view/158662/153679
dc.rights.driver.fl_str_mv Copyright (c) 2019 Journal of Applied Oral Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Journal of Applied Oral Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Odontologia de Bauru
dc.source.none.fl_str_mv Journal of Applied Oral Science; Vol. 27 (2019); e20180205
Journal of Applied Oral Science; Vol. 27 (2019); e20180205
Journal of Applied Oral Science; v. 27 (2019); e20180205
1678-7765
1678-7757
reponame:Journal of applied oral science (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Journal of applied oral science (Online)
collection Journal of applied oral science (Online)
repository.name.fl_str_mv Journal of applied oral science (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||jaos@usp.br
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