Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats

Detalhes bibliográficos
Autor(a) principal: Bianchi, Paula Cristina [UNESP]
Data de Publicação: 2018
Outros Autores: Carneiro de Oliveira, Paulo Eduardo [UNESP], Palombo, Paola [UNESP], Leao, Rodrigo Molini, Cogo-Moreira, Hugo, Planeta, Cleopatra da Silva [UNESP], Cruz, Fabio Cardoso
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.drugalcdep.2017.12.045
http://hdl.handle.net/11449/164808
Resumo: Background: The high rate of relapse to drug use remains a central challenge to treating drug addiction. In human and rat models of addiction, environmental stimuli in contexts associated with previous drug use can provoke a relapse of drug seeking. Pre-clinical studies have used the ABA renewal procedure to study context induced reinstatement of drug seeking. In the current study, we studied the role of the orbitofrontal cortex (OFC) in context-induced reinstatement to alcohol. Methods: We trained male and female rats to self-administer alcohol in context A, extinguished drug-reinforced responding in a distinct context B, and assessed context-induced reinstatement in context A or B (control group). Next, we determined the effect of context-induced renewal of alcohol-seeking behavior on the expression of Fos (a neuronal activity marker) in the OFC. Finally, we determined the effect of reversible inactivation by GABAa and GABAb receptor agonists (i.e., muscimol and baclofen, respectively) in the OFC. Results and conclusions: There were no differences between male and female rats in context-induced reinstatement of alcohol-seeking behavior. Re-exposure to Context A, but not Context B, reinstated alcohol-seeking behavior and increased expression of the neural activity marker Fos in the OFC. Reversible inactivation of the OFC with muscimol and baclofen attenuated context-induced reinstatement. Our data indicated that the OFC mediates context-induced reinstatement of alcohol-seeking behavior.
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spelling Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in ratsAlcohol self-administrationOrbitofrontal cortexMale and female ratsFunctional inactivationBackground: The high rate of relapse to drug use remains a central challenge to treating drug addiction. In human and rat models of addiction, environmental stimuli in contexts associated with previous drug use can provoke a relapse of drug seeking. Pre-clinical studies have used the ABA renewal procedure to study context induced reinstatement of drug seeking. In the current study, we studied the role of the orbitofrontal cortex (OFC) in context-induced reinstatement to alcohol. Methods: We trained male and female rats to self-administer alcohol in context A, extinguished drug-reinforced responding in a distinct context B, and assessed context-induced reinstatement in context A or B (control group). Next, we determined the effect of context-induced renewal of alcohol-seeking behavior on the expression of Fos (a neuronal activity marker) in the OFC. Finally, we determined the effect of reversible inactivation by GABAa and GABAb receptor agonists (i.e., muscimol and baclofen, respectively) in the OFC. Results and conclusions: There were no differences between male and female rats in context-induced reinstatement of alcohol-seeking behavior. Re-exposure to Context A, but not Context B, reinstated alcohol-seeking behavior and increased expression of the neural activity marker Fos in the OFC. Reversible inactivation of the OFC with muscimol and baclofen attenuated context-induced reinstatement. Our data indicated that the OFC mediates context-induced reinstatement of alcohol-seeking behavior.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sao Paulo State Univ, Lab Pharmacol, Rod Araraquara Jau Km 1, BR-14801902 Araraquara, SP, BrazilUniv Fed Sao Carlos, Joint Grad Program Physiol Sci, Rod Washington Luis Km 235, BR-13565905 Sao Carlos, SP, BrazilSao Paulo State Univ Araraquara, Rod Washington Luis Km 235, BR-13565905 Sao Carlos, SP, BrazilUniv Fed Bahia, UFBA, Bioregulat Dept, Av Reitor Miguel Calmon S-N, Salvador, BA, BrazilSao Paulo Fed Univ, Dept Psychiat, R Dr Ovidio Pires de Campos, BR-78505403 Sao Paulo, SP, BrazilSao Paulo Fed Univ, Dept Pharmacol, Leal Prado Bldg,Botucatu 862 St, BR-04024002 Sao Paulo, SP, BrazilSao Paulo State Univ, Lab Pharmacol, Rod Araraquara Jau Km 1, BR-14801902 Araraquara, SP, BrazilSao Paulo State Univ Araraquara, Rod Washington Luis Km 235, BR-13565905 Sao Carlos, SP, BrazilFAPESP: 2013/24896-2CNPq: 164060/2014-6Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Universidade Federal da Bahia (UFBA)Sao Paulo Fed UnivBianchi, Paula Cristina [UNESP]Carneiro de Oliveira, Paulo Eduardo [UNESP]Palombo, Paola [UNESP]Leao, Rodrigo MoliniCogo-Moreira, HugoPlaneta, Cleopatra da Silva [UNESP]Cruz, Fabio Cardoso2018-11-26T22:38:13Z2018-11-26T22:38:13Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article102-112application/pdfhttp://dx.doi.org/10.1016/j.drugalcdep.2017.12.045Drug And Alcohol Dependence. Clare: Elsevier Ireland Ltd, v. 186, p. 102-112, 2018.0376-8716http://hdl.handle.net/11449/16480810.1016/j.drugalcdep.2017.12.045WOS:000432616400016WOS000432616400016.pdf25147625452809420000-0002-1378-6327Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDrug And Alcohol Dependence1,715info:eu-repo/semantics/openAccess2024-01-14T06:18:32Zoai:repositorio.unesp.br:11449/164808Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:55:33.307900Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats
title Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats
spellingShingle Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats
Bianchi, Paula Cristina [UNESP]
Alcohol self-administration
Orbitofrontal cortex
Male and female rats
Functional inactivation
title_short Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats
title_full Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats
title_fullStr Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats
title_full_unstemmed Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats
title_sort Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats
author Bianchi, Paula Cristina [UNESP]
author_facet Bianchi, Paula Cristina [UNESP]
Carneiro de Oliveira, Paulo Eduardo [UNESP]
Palombo, Paola [UNESP]
Leao, Rodrigo Molini
Cogo-Moreira, Hugo
Planeta, Cleopatra da Silva [UNESP]
Cruz, Fabio Cardoso
author_role author
author2 Carneiro de Oliveira, Paulo Eduardo [UNESP]
Palombo, Paola [UNESP]
Leao, Rodrigo Molini
Cogo-Moreira, Hugo
Planeta, Cleopatra da Silva [UNESP]
Cruz, Fabio Cardoso
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de São Carlos (UFSCar)
Universidade Federal da Bahia (UFBA)
Sao Paulo Fed Univ
dc.contributor.author.fl_str_mv Bianchi, Paula Cristina [UNESP]
Carneiro de Oliveira, Paulo Eduardo [UNESP]
Palombo, Paola [UNESP]
Leao, Rodrigo Molini
Cogo-Moreira, Hugo
Planeta, Cleopatra da Silva [UNESP]
Cruz, Fabio Cardoso
dc.subject.por.fl_str_mv Alcohol self-administration
Orbitofrontal cortex
Male and female rats
Functional inactivation
topic Alcohol self-administration
Orbitofrontal cortex
Male and female rats
Functional inactivation
description Background: The high rate of relapse to drug use remains a central challenge to treating drug addiction. In human and rat models of addiction, environmental stimuli in contexts associated with previous drug use can provoke a relapse of drug seeking. Pre-clinical studies have used the ABA renewal procedure to study context induced reinstatement of drug seeking. In the current study, we studied the role of the orbitofrontal cortex (OFC) in context-induced reinstatement to alcohol. Methods: We trained male and female rats to self-administer alcohol in context A, extinguished drug-reinforced responding in a distinct context B, and assessed context-induced reinstatement in context A or B (control group). Next, we determined the effect of context-induced renewal of alcohol-seeking behavior on the expression of Fos (a neuronal activity marker) in the OFC. Finally, we determined the effect of reversible inactivation by GABAa and GABAb receptor agonists (i.e., muscimol and baclofen, respectively) in the OFC. Results and conclusions: There were no differences between male and female rats in context-induced reinstatement of alcohol-seeking behavior. Re-exposure to Context A, but not Context B, reinstated alcohol-seeking behavior and increased expression of the neural activity marker Fos in the OFC. Reversible inactivation of the OFC with muscimol and baclofen attenuated context-induced reinstatement. Our data indicated that the OFC mediates context-induced reinstatement of alcohol-seeking behavior.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-26T22:38:13Z
2018-11-26T22:38:13Z
2018-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.drugalcdep.2017.12.045
Drug And Alcohol Dependence. Clare: Elsevier Ireland Ltd, v. 186, p. 102-112, 2018.
0376-8716
http://hdl.handle.net/11449/164808
10.1016/j.drugalcdep.2017.12.045
WOS:000432616400016
WOS000432616400016.pdf
2514762545280942
0000-0002-1378-6327
url http://dx.doi.org/10.1016/j.drugalcdep.2017.12.045
http://hdl.handle.net/11449/164808
identifier_str_mv Drug And Alcohol Dependence. Clare: Elsevier Ireland Ltd, v. 186, p. 102-112, 2018.
0376-8716
10.1016/j.drugalcdep.2017.12.045
WOS:000432616400016
WOS000432616400016.pdf
2514762545280942
0000-0002-1378-6327
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Drug And Alcohol Dependence
1,715
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 102-112
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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