Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.drugalcdep.2017.12.045 http://hdl.handle.net/11449/164808 |
Resumo: | Background: The high rate of relapse to drug use remains a central challenge to treating drug addiction. In human and rat models of addiction, environmental stimuli in contexts associated with previous drug use can provoke a relapse of drug seeking. Pre-clinical studies have used the ABA renewal procedure to study context induced reinstatement of drug seeking. In the current study, we studied the role of the orbitofrontal cortex (OFC) in context-induced reinstatement to alcohol. Methods: We trained male and female rats to self-administer alcohol in context A, extinguished drug-reinforced responding in a distinct context B, and assessed context-induced reinstatement in context A or B (control group). Next, we determined the effect of context-induced renewal of alcohol-seeking behavior on the expression of Fos (a neuronal activity marker) in the OFC. Finally, we determined the effect of reversible inactivation by GABAa and GABAb receptor agonists (i.e., muscimol and baclofen, respectively) in the OFC. Results and conclusions: There were no differences between male and female rats in context-induced reinstatement of alcohol-seeking behavior. Re-exposure to Context A, but not Context B, reinstated alcohol-seeking behavior and increased expression of the neural activity marker Fos in the OFC. Reversible inactivation of the OFC with muscimol and baclofen attenuated context-induced reinstatement. Our data indicated that the OFC mediates context-induced reinstatement of alcohol-seeking behavior. |
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Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in ratsAlcohol self-administrationOrbitofrontal cortexMale and female ratsFunctional inactivationBackground: The high rate of relapse to drug use remains a central challenge to treating drug addiction. In human and rat models of addiction, environmental stimuli in contexts associated with previous drug use can provoke a relapse of drug seeking. Pre-clinical studies have used the ABA renewal procedure to study context induced reinstatement of drug seeking. In the current study, we studied the role of the orbitofrontal cortex (OFC) in context-induced reinstatement to alcohol. Methods: We trained male and female rats to self-administer alcohol in context A, extinguished drug-reinforced responding in a distinct context B, and assessed context-induced reinstatement in context A or B (control group). Next, we determined the effect of context-induced renewal of alcohol-seeking behavior on the expression of Fos (a neuronal activity marker) in the OFC. Finally, we determined the effect of reversible inactivation by GABAa and GABAb receptor agonists (i.e., muscimol and baclofen, respectively) in the OFC. Results and conclusions: There were no differences between male and female rats in context-induced reinstatement of alcohol-seeking behavior. Re-exposure to Context A, but not Context B, reinstated alcohol-seeking behavior and increased expression of the neural activity marker Fos in the OFC. Reversible inactivation of the OFC with muscimol and baclofen attenuated context-induced reinstatement. Our data indicated that the OFC mediates context-induced reinstatement of alcohol-seeking behavior.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Sao Paulo State Univ, Lab Pharmacol, Rod Araraquara Jau Km 1, BR-14801902 Araraquara, SP, BrazilUniv Fed Sao Carlos, Joint Grad Program Physiol Sci, Rod Washington Luis Km 235, BR-13565905 Sao Carlos, SP, BrazilSao Paulo State Univ Araraquara, Rod Washington Luis Km 235, BR-13565905 Sao Carlos, SP, BrazilUniv Fed Bahia, UFBA, Bioregulat Dept, Av Reitor Miguel Calmon S-N, Salvador, BA, BrazilSao Paulo Fed Univ, Dept Psychiat, R Dr Ovidio Pires de Campos, BR-78505403 Sao Paulo, SP, BrazilSao Paulo Fed Univ, Dept Pharmacol, Leal Prado Bldg,Botucatu 862 St, BR-04024002 Sao Paulo, SP, BrazilSao Paulo State Univ, Lab Pharmacol, Rod Araraquara Jau Km 1, BR-14801902 Araraquara, SP, BrazilSao Paulo State Univ Araraquara, Rod Washington Luis Km 235, BR-13565905 Sao Carlos, SP, BrazilFAPESP: 2013/24896-2CNPq: 164060/2014-6Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Universidade Federal da Bahia (UFBA)Sao Paulo Fed UnivBianchi, Paula Cristina [UNESP]Carneiro de Oliveira, Paulo Eduardo [UNESP]Palombo, Paola [UNESP]Leao, Rodrigo MoliniCogo-Moreira, HugoPlaneta, Cleopatra da Silva [UNESP]Cruz, Fabio Cardoso2018-11-26T22:38:13Z2018-11-26T22:38:13Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article102-112application/pdfhttp://dx.doi.org/10.1016/j.drugalcdep.2017.12.045Drug And Alcohol Dependence. Clare: Elsevier Ireland Ltd, v. 186, p. 102-112, 2018.0376-8716http://hdl.handle.net/11449/16480810.1016/j.drugalcdep.2017.12.045WOS:000432616400016WOS000432616400016.pdf25147625452809420000-0002-1378-6327Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDrug And Alcohol Dependence1,715info:eu-repo/semantics/openAccess2024-01-14T06:18:32Zoai:repositorio.unesp.br:11449/164808Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:55:33.307900Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats |
title |
Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats |
spellingShingle |
Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats Bianchi, Paula Cristina [UNESP] Alcohol self-administration Orbitofrontal cortex Male and female rats Functional inactivation |
title_short |
Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats |
title_full |
Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats |
title_fullStr |
Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats |
title_full_unstemmed |
Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats |
title_sort |
Functional inactivation of the orbitofrontal cortex disrupts context-induced reinstatement of alcohol seeking in rats |
author |
Bianchi, Paula Cristina [UNESP] |
author_facet |
Bianchi, Paula Cristina [UNESP] Carneiro de Oliveira, Paulo Eduardo [UNESP] Palombo, Paola [UNESP] Leao, Rodrigo Molini Cogo-Moreira, Hugo Planeta, Cleopatra da Silva [UNESP] Cruz, Fabio Cardoso |
author_role |
author |
author2 |
Carneiro de Oliveira, Paulo Eduardo [UNESP] Palombo, Paola [UNESP] Leao, Rodrigo Molini Cogo-Moreira, Hugo Planeta, Cleopatra da Silva [UNESP] Cruz, Fabio Cardoso |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) Universidade Federal da Bahia (UFBA) Sao Paulo Fed Univ |
dc.contributor.author.fl_str_mv |
Bianchi, Paula Cristina [UNESP] Carneiro de Oliveira, Paulo Eduardo [UNESP] Palombo, Paola [UNESP] Leao, Rodrigo Molini Cogo-Moreira, Hugo Planeta, Cleopatra da Silva [UNESP] Cruz, Fabio Cardoso |
dc.subject.por.fl_str_mv |
Alcohol self-administration Orbitofrontal cortex Male and female rats Functional inactivation |
topic |
Alcohol self-administration Orbitofrontal cortex Male and female rats Functional inactivation |
description |
Background: The high rate of relapse to drug use remains a central challenge to treating drug addiction. In human and rat models of addiction, environmental stimuli in contexts associated with previous drug use can provoke a relapse of drug seeking. Pre-clinical studies have used the ABA renewal procedure to study context induced reinstatement of drug seeking. In the current study, we studied the role of the orbitofrontal cortex (OFC) in context-induced reinstatement to alcohol. Methods: We trained male and female rats to self-administer alcohol in context A, extinguished drug-reinforced responding in a distinct context B, and assessed context-induced reinstatement in context A or B (control group). Next, we determined the effect of context-induced renewal of alcohol-seeking behavior on the expression of Fos (a neuronal activity marker) in the OFC. Finally, we determined the effect of reversible inactivation by GABAa and GABAb receptor agonists (i.e., muscimol and baclofen, respectively) in the OFC. Results and conclusions: There were no differences between male and female rats in context-induced reinstatement of alcohol-seeking behavior. Re-exposure to Context A, but not Context B, reinstated alcohol-seeking behavior and increased expression of the neural activity marker Fos in the OFC. Reversible inactivation of the OFC with muscimol and baclofen attenuated context-induced reinstatement. Our data indicated that the OFC mediates context-induced reinstatement of alcohol-seeking behavior. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T22:38:13Z 2018-11-26T22:38:13Z 2018-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.drugalcdep.2017.12.045 Drug And Alcohol Dependence. Clare: Elsevier Ireland Ltd, v. 186, p. 102-112, 2018. 0376-8716 http://hdl.handle.net/11449/164808 10.1016/j.drugalcdep.2017.12.045 WOS:000432616400016 WOS000432616400016.pdf 2514762545280942 0000-0002-1378-6327 |
url |
http://dx.doi.org/10.1016/j.drugalcdep.2017.12.045 http://hdl.handle.net/11449/164808 |
identifier_str_mv |
Drug And Alcohol Dependence. Clare: Elsevier Ireland Ltd, v. 186, p. 102-112, 2018. 0376-8716 10.1016/j.drugalcdep.2017.12.045 WOS:000432616400016 WOS000432616400016.pdf 2514762545280942 0000-0002-1378-6327 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Drug And Alcohol Dependence 1,715 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
102-112 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129473992720384 |