Efeito da desnutrição proteica perinatal sobre a população de neurônios sintetizadores das proteínas ligantes de cálcio calbindina, calretinina e parvalbumina no córtex pré-frontal de ratos

• Autor(a) principal: Paiva, Isabela Rogério de [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/155200; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-05-03/000880810.pdf
• topic: Córtex pré-frontal; Desnutrição; Esquizofrenia; Neuronios; Calbindina 2; Ratos; Prefrontal cortex

Perinatal protein malnutrition (PPM) is considered a serious public health concern, affecting a significant portion of the world's population. Epidemiological studies indicate that protein perinatal deprivation may represent risk factors for the late onset of some mental and psychiatric disorders, fundamentally schizophrenia, condition where the prefrontal cortex (PFC) plays a fundamental role. In view of the correlation between these neuropsychiatric conditions and PPM, it remains to be determined if some of the structural changes found mainly in the PFC of patients in these situations may be reproduced in PPM experimental models. Between the structural changes observed in schizophrenic patients, it has been reported alterations in the PFC volume and in the GABAergic system, including here neurons that also express the calcium binding proteins (CBPs) parvalbumin, calbindin and calretinin. Thus, in the present study, using a model PPM in rats, immunohistochemical techniques and unbiased stereological analysis we quantify the PFC volume, the overall neuronal population evidenced by NeuN immunohistochemistry, as well as the calbindin (Cb +), calretinin (Cr +) and parvalbumin (PV +) immunorecative neurons, in the rat PFC at P21 and P60. Free-floating sections were processed for NeuN and CBPs immunohistochemistry. The total neuron numbers was estimated using the optical fractionator, with the region of interest (ROI) including all layers and areas of the PFC. Our stereological analysis showed that PPM did not significantly alter the number of NeuN+ neurons in the rat mPFC in any of the ages studied. However, we observed a significant decrease in the PFC volume as well as in the number of Pv+ neurons in the PFC at P21. Considering that there are no differences in the total number of neurons in the mPFC of control and malnourished rats, the changes found in the number of Pv+ neurons indicate a decrease...