Quantificação estereológica de neurônios reativos às proteínas ligantes de cálcio calbindina, calretinina e parvalbumina no córtex pré-frontal de ratos submetidos a anóxia neonatal

• Autor(a) principal: Pimenta, Taís Fernandes [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/155209; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-05-08/000880904.pdf
• topic: Córtex pré-frontal; Proteínas de ligação ao cálcio; Calbindina 2; Estereologia; Parvalbuminas; Prefrontal cortex

Hypoxic (NH) or anoxic (NA) neonatal events, where there is a decrease in the oxygen content of organic tissues, represent a major public health concern. Recent studies indicate that hypoxic / anoxic events during childbirth, besides other immediate consequences, may represent risk factors for late onset of psychiatric conditions in which the prefrontal cortex plays a fundamental role, as the Deficit Disorder Attention Deficit Hyperactivity Disorder (ADHD), autism, and schizophrenia. This study aims to establish if the structural changes observed in the prefrontal cortex of patients with these medical conditions can be reproduced in experimental models of NA. For this, using a model of NA in rats and unbiased stereological techniques, we analyzed cellular populations of the PFC commonly affected in these situations. This analysis included the quantification of the Calcium Binding Proteins calbindin, calretinin and parvalbumin. Our results indicate a significant decrease in the parvalbumin positive neurons at P21 in anoxic animals, with subsequent statistical normalization at P60. Calbindin and calretinin populations showed no significant differences between anoxic and control animals