Efeito fotodinâmico da curcumina no tratamento da periodontite induzida em ratos: estudo histométrico

• Autor(a) principal: Ferro-Alves, Márcio Luiz [UNESP]
• Data de Publicação: 2013
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149612; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-30/000856487.pdf
• topic: Curcumina; Periodontite; Ratos; Curcumin

The objective of this study was to evaluate the viewpoint histometric the influence of curcumin as a photosensitizing agent associated with LED in irradiating APDT (antimicrobial photodynamic therapy) for the treatment of experimentally induced periodontitis in rats. Ninety-six animals were subjected to induction of periodontitis installation via a cotton thread around the first molars, which was maintained for 7 days. Then the animals were randomly divided into 4 groups of 24 animals each, which received the following treatments: G1 (n = 24) - represented by animals suffered no local treatment G2 (n = 24) - irrigation solution curcumin (40μM), G3 (n = 24) - irradiation with LED (Light emitting diode), G4 (n = 24) - irrigation with a solution of curcumin (40μM) followed by irradiation with LED. Local treatments were carried out 7 days after the induction of periodontitis. The LED, which was used in this study to achieve the APDT is a semiconductor emitter of indium gallium nitridium ( InGaN ) with a wavelength of 465 to 485nm and irradiance of 600mW/cm ². The LED irradiation was performed for 60 seconds in the buccal surface of the first lower molar on the left. Treatment with the LED G4 was performed after 60 seconds of irrigation with the photosensitizer curcumin . The animals, eight in number for each group / time were sacrificed at intervals of 7, 15 and 30 days after treatment. After euthanasia, the specimens were histologically processed and stained with hematoxylin and eosin technique. We assessed the OPs Histometry in the furcation area of the first molar on the left side. The data of bone loss were analyzed statistically with a significance level of 5% (ANOVA and Tukey test). The comparative analysis of the measures between the treatments, it was observed that there was a greater alveolar bone loss in the furcation in G1 at 15...