Fertility of male adult rats submitted to forced swimming stress

Detalhes bibliográficos
Autor(a) principal: Mingoti, Gisele Zoccal [UNESP]
Data de Publicação: 2003
Outros Autores: Pereira, R. N. [UNESP], Monteiro, Cristina Maria Rodrigues [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-879X2003000500016
http://hdl.handle.net/11449/28070
Resumo: We investigated whether stress interferes with fertility during adulthood. Male Wistar rats (weighing 220 g in the beginning of the experiment) were forced to swim for 3 min in water at 32ºC daily for 15 days. Stress was assessed by the hot-plate test after the last stressing session. To assess fertility, control and stressed males (N = 15 per group) were mated with sexually mature normal females. Males were sacrificed after copulation. Stress caused by forced swimming was demonstrated by a significant increase in the latency of the pain response in the hot-plate test (14.6 ± 1.25 s for control males vs 26.0 ± 1.53 s for stressed males, P = 0.0004). No changes were observed in body weight, testicular weight, seminal vesicle weight, ventral prostate weight or gross histological features of the testes of stressed males. Similarly, no changes were observed in fertility rate, measured by counting live fetuses in the uterus of normal females mated with control and stressed males; no dead or incompletely developed fetuses were observed in the uterus of either group. In contrast, there was a statistically significant decrease in spermatid production demonstrated by histometric evaluation (154.96 ± 5.41 vs 127.02 ± 3.95 spermatids per tubular section for control and stressed rats, respectively, P = 0.001). These data demonstrate that 15 days of forced swimming stress applied to adult male rats did not impair fertility, but significantly decreased spermatid production. This suggests that the effect of stress on fertility should not be assessed before at least the time required for one cycle of spermatogenesis.
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spelling Fertility of male adult rats submitted to forced swimming stressForced swimming stressMaleFertilityRatSpermatogoniaWe investigated whether stress interferes with fertility during adulthood. Male Wistar rats (weighing 220 g in the beginning of the experiment) were forced to swim for 3 min in water at 32ºC daily for 15 days. Stress was assessed by the hot-plate test after the last stressing session. To assess fertility, control and stressed males (N = 15 per group) were mated with sexually mature normal females. Males were sacrificed after copulation. Stress caused by forced swimming was demonstrated by a significant increase in the latency of the pain response in the hot-plate test (14.6 ± 1.25 s for control males vs 26.0 ± 1.53 s for stressed males, P = 0.0004). No changes were observed in body weight, testicular weight, seminal vesicle weight, ventral prostate weight or gross histological features of the testes of stressed males. Similarly, no changes were observed in fertility rate, measured by counting live fetuses in the uterus of normal females mated with control and stressed males; no dead or incompletely developed fetuses were observed in the uterus of either group. In contrast, there was a statistically significant decrease in spermatid production demonstrated by histometric evaluation (154.96 ± 5.41 vs 127.02 ± 3.95 spermatids per tubular section for control and stressed rats, respectively, P = 0.001). These data demonstrate that 15 days of forced swimming stress applied to adult male rats did not impair fertility, but significantly decreased spermatid production. This suggests that the effect of stress on fertility should not be assessed before at least the time required for one cycle of spermatogenesis.Universidade Estadual Paulista Medicina Veterinária Departamento de Produção e Saúde AnimalUniversidade Estadual Paulista Medicina Veterinária Departamento de Produção e Saúde AnimalAssociação Brasileira de Divulgação Científica (ABRADIC)Universidade Estadual Paulista (Unesp)Mingoti, Gisele Zoccal [UNESP]Pereira, R. N. [UNESP]Monteiro, Cristina Maria Rodrigues [UNESP]2014-05-20T15:11:32Z2014-05-20T15:11:32Z2003-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article677-682application/pdfhttp://dx.doi.org/10.1590/S0100-879X2003000500016Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 5, p. 677-682, 2003.0100-879Xhttp://hdl.handle.net/11449/2807010.1590/S0100-879X2003000500016S0100-879X2003000500016S0100-879X2003000500016.pdf419195176433476665863313433543430000-0002-3059-4458SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Medical and Biological Research1.492info:eu-repo/semantics/openAccess2024-09-04T19:15:26Zoai:repositorio.unesp.br:11449/28070Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Fertility of male adult rats submitted to forced swimming stress
title Fertility of male adult rats submitted to forced swimming stress
spellingShingle Fertility of male adult rats submitted to forced swimming stress
Mingoti, Gisele Zoccal [UNESP]
Forced swimming stress
Male
Fertility
Rat
Spermatogonia
title_short Fertility of male adult rats submitted to forced swimming stress
title_full Fertility of male adult rats submitted to forced swimming stress
title_fullStr Fertility of male adult rats submitted to forced swimming stress
title_full_unstemmed Fertility of male adult rats submitted to forced swimming stress
title_sort Fertility of male adult rats submitted to forced swimming stress
author Mingoti, Gisele Zoccal [UNESP]
author_facet Mingoti, Gisele Zoccal [UNESP]
Pereira, R. N. [UNESP]
Monteiro, Cristina Maria Rodrigues [UNESP]
author_role author
author2 Pereira, R. N. [UNESP]
Monteiro, Cristina Maria Rodrigues [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Mingoti, Gisele Zoccal [UNESP]
Pereira, R. N. [UNESP]
Monteiro, Cristina Maria Rodrigues [UNESP]
dc.subject.por.fl_str_mv Forced swimming stress
Male
Fertility
Rat
Spermatogonia
topic Forced swimming stress
Male
Fertility
Rat
Spermatogonia
description We investigated whether stress interferes with fertility during adulthood. Male Wistar rats (weighing 220 g in the beginning of the experiment) were forced to swim for 3 min in water at 32ºC daily for 15 days. Stress was assessed by the hot-plate test after the last stressing session. To assess fertility, control and stressed males (N = 15 per group) were mated with sexually mature normal females. Males were sacrificed after copulation. Stress caused by forced swimming was demonstrated by a significant increase in the latency of the pain response in the hot-plate test (14.6 ± 1.25 s for control males vs 26.0 ± 1.53 s for stressed males, P = 0.0004). No changes were observed in body weight, testicular weight, seminal vesicle weight, ventral prostate weight or gross histological features of the testes of stressed males. Similarly, no changes were observed in fertility rate, measured by counting live fetuses in the uterus of normal females mated with control and stressed males; no dead or incompletely developed fetuses were observed in the uterus of either group. In contrast, there was a statistically significant decrease in spermatid production demonstrated by histometric evaluation (154.96 ± 5.41 vs 127.02 ± 3.95 spermatids per tubular section for control and stressed rats, respectively, P = 0.001). These data demonstrate that 15 days of forced swimming stress applied to adult male rats did not impair fertility, but significantly decreased spermatid production. This suggests that the effect of stress on fertility should not be assessed before at least the time required for one cycle of spermatogenesis.
publishDate 2003
dc.date.none.fl_str_mv 2003-05-01
2014-05-20T15:11:32Z
2014-05-20T15:11:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-879X2003000500016
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 5, p. 677-682, 2003.
0100-879X
http://hdl.handle.net/11449/28070
10.1590/S0100-879X2003000500016
S0100-879X2003000500016
S0100-879X2003000500016.pdf
4191951764334766
6586331343354343
0000-0002-3059-4458
url http://dx.doi.org/10.1590/S0100-879X2003000500016
http://hdl.handle.net/11449/28070
identifier_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 5, p. 677-682, 2003.
0100-879X
10.1590/S0100-879X2003000500016
S0100-879X2003000500016
S0100-879X2003000500016.pdf
4191951764334766
6586331343354343
0000-0002-3059-4458
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
1.492
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 677-682
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica (ABRADIC)
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica (ABRADIC)
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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