Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats

Detalhes bibliográficos
Autor(a) principal: Martinez, M.
Data de Publicação: 2019
Outros Autores: Rossetto, I. M.U., Arantes, R. M.S., Lizarte, F. S.N., Tirapelli, L. F., Tirapelli, D. P.C., Chuffa, L. G.A. [UNESP], Martinez, F. E. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1039/c9tx00069k
http://hdl.handle.net/11449/198277
Resumo: Alcoholism is a multifactorial disease with high risk for dependence determined by genetic background, environmental factors and neuroadaptations. The excessive consumption of this substance is related to psychiatric problems, epilepsy, cardiovascular disease, cirrhosis and cancers. Caffeine is one of the most popular psychostimulants currently consumed in the world. The combination of ethanol and caffeine ingested by consuming energy drinks is becoming increasingly popular among young people. We analyzed the effect of simultaneous consumption of ethanol and caffeine on the serum profile of miRNAs differentially expressed in the ethanol-drinking rat model (UChB strain). Adult rats were divided into three groups (n = 5 per group): UChB group (rats fed with 1 : 10 (v/v) ethanol ad libitum); UChB + caffeine group (rats fed with 1 : 10 (v/v) ethanol ad libitum + 3 g L-1 of caffeine); control group (rats drinking water used as the control for UChB). The treatment with caffeine occurred from day 95 to 150 days old, totalizing 55 days of ethanol + caffeine ingestion. The expressions of microRNAs (miR) -9-3p, -15b-5p, -16-5p, -21-5p, -200a-3p and -222-3p were detected by Real Time-PCR (RT-PCR). The expressions of miR-9-3p, -15b-5p, -16-5p and -222-3p were upregulated in the UChB group. Conversely, simultaneous ingestion of ethanol and caffeine significantly reversed these expressions to similar levels to control animals, thus emphasizing that caffeine had a protective effect in the presence of ethanol. In addition, miR-21-5p was downregulated with ethanol consumption whereas miR-222-3p was unchanged. Ethanol and caffeine consumption was capable of altering serum miRNAs, which are potential biomarkers for the systemic effects of these addictive substances.
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spelling Serum miRNAs are differentially altered by ethanol and caffeine consumption in ratsAlcoholism is a multifactorial disease with high risk for dependence determined by genetic background, environmental factors and neuroadaptations. The excessive consumption of this substance is related to psychiatric problems, epilepsy, cardiovascular disease, cirrhosis and cancers. Caffeine is one of the most popular psychostimulants currently consumed in the world. The combination of ethanol and caffeine ingested by consuming energy drinks is becoming increasingly popular among young people. We analyzed the effect of simultaneous consumption of ethanol and caffeine on the serum profile of miRNAs differentially expressed in the ethanol-drinking rat model (UChB strain). Adult rats were divided into three groups (n = 5 per group): UChB group (rats fed with 1 : 10 (v/v) ethanol ad libitum); UChB + caffeine group (rats fed with 1 : 10 (v/v) ethanol ad libitum + 3 g L-1 of caffeine); control group (rats drinking water used as the control for UChB). The treatment with caffeine occurred from day 95 to 150 days old, totalizing 55 days of ethanol + caffeine ingestion. The expressions of microRNAs (miR) -9-3p, -15b-5p, -16-5p, -21-5p, -200a-3p and -222-3p were detected by Real Time-PCR (RT-PCR). The expressions of miR-9-3p, -15b-5p, -16-5p and -222-3p were upregulated in the UChB group. Conversely, simultaneous ingestion of ethanol and caffeine significantly reversed these expressions to similar levels to control animals, thus emphasizing that caffeine had a protective effect in the presence of ethanol. In addition, miR-21-5p was downregulated with ethanol consumption whereas miR-222-3p was unchanged. Ethanol and caffeine consumption was capable of altering serum miRNAs, which are potential biomarkers for the systemic effects of these addictive substances.Department of Morphology and Pathology Federal University of São Carlos (UFSCar)Department Structural and Functional Biology University of Campinas (UNICAMP)Department of Surgery and Anatomy University of São Paulo (USP)Department of Anatomy State University of São Paulo (UNESP)Department of Anatomy State University of São Paulo (UNESP)Universidade Estadual Paulista (Unesp)Martinez, M.Rossetto, I. M.U.Arantes, R. M.S.Lizarte, F. S.N.Tirapelli, L. F.Tirapelli, D. P.C.Chuffa, L. G.A. [UNESP]Martinez, F. E. [UNESP]2020-12-12T01:08:23Z2020-12-12T01:08:23Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article842-849http://dx.doi.org/10.1039/c9tx00069kToxicology Research, v. 8, n. 6, p. 842-849, 2019.2045-45382045-452Xhttp://hdl.handle.net/11449/19827710.1039/c9tx00069k2-s2.0-85076484406Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxicology Researchinfo:eu-repo/semantics/openAccess2021-10-23T10:11:21Zoai:repositorio.unesp.br:11449/198277Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:14:20.324532Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats
title Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats
spellingShingle Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats
Martinez, M.
title_short Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats
title_full Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats
title_fullStr Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats
title_full_unstemmed Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats
title_sort Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats
author Martinez, M.
author_facet Martinez, M.
Rossetto, I. M.U.
Arantes, R. M.S.
Lizarte, F. S.N.
Tirapelli, L. F.
Tirapelli, D. P.C.
Chuffa, L. G.A. [UNESP]
Martinez, F. E. [UNESP]
author_role author
author2 Rossetto, I. M.U.
Arantes, R. M.S.
Lizarte, F. S.N.
Tirapelli, L. F.
Tirapelli, D. P.C.
Chuffa, L. G.A. [UNESP]
Martinez, F. E. [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Martinez, M.
Rossetto, I. M.U.
Arantes, R. M.S.
Lizarte, F. S.N.
Tirapelli, L. F.
Tirapelli, D. P.C.
Chuffa, L. G.A. [UNESP]
Martinez, F. E. [UNESP]
description Alcoholism is a multifactorial disease with high risk for dependence determined by genetic background, environmental factors and neuroadaptations. The excessive consumption of this substance is related to psychiatric problems, epilepsy, cardiovascular disease, cirrhosis and cancers. Caffeine is one of the most popular psychostimulants currently consumed in the world. The combination of ethanol and caffeine ingested by consuming energy drinks is becoming increasingly popular among young people. We analyzed the effect of simultaneous consumption of ethanol and caffeine on the serum profile of miRNAs differentially expressed in the ethanol-drinking rat model (UChB strain). Adult rats were divided into three groups (n = 5 per group): UChB group (rats fed with 1 : 10 (v/v) ethanol ad libitum); UChB + caffeine group (rats fed with 1 : 10 (v/v) ethanol ad libitum + 3 g L-1 of caffeine); control group (rats drinking water used as the control for UChB). The treatment with caffeine occurred from day 95 to 150 days old, totalizing 55 days of ethanol + caffeine ingestion. The expressions of microRNAs (miR) -9-3p, -15b-5p, -16-5p, -21-5p, -200a-3p and -222-3p were detected by Real Time-PCR (RT-PCR). The expressions of miR-9-3p, -15b-5p, -16-5p and -222-3p were upregulated in the UChB group. Conversely, simultaneous ingestion of ethanol and caffeine significantly reversed these expressions to similar levels to control animals, thus emphasizing that caffeine had a protective effect in the presence of ethanol. In addition, miR-21-5p was downregulated with ethanol consumption whereas miR-222-3p was unchanged. Ethanol and caffeine consumption was capable of altering serum miRNAs, which are potential biomarkers for the systemic effects of these addictive substances.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
2020-12-12T01:08:23Z
2020-12-12T01:08:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1039/c9tx00069k
Toxicology Research, v. 8, n. 6, p. 842-849, 2019.
2045-4538
2045-452X
http://hdl.handle.net/11449/198277
10.1039/c9tx00069k
2-s2.0-85076484406
url http://dx.doi.org/10.1039/c9tx00069k
http://hdl.handle.net/11449/198277
identifier_str_mv Toxicology Research, v. 8, n. 6, p. 842-849, 2019.
2045-4538
2045-452X
10.1039/c9tx00069k
2-s2.0-85076484406
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxicology Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 842-849
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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