Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1039/c9tx00069k http://hdl.handle.net/11449/198277 |
Resumo: | Alcoholism is a multifactorial disease with high risk for dependence determined by genetic background, environmental factors and neuroadaptations. The excessive consumption of this substance is related to psychiatric problems, epilepsy, cardiovascular disease, cirrhosis and cancers. Caffeine is one of the most popular psychostimulants currently consumed in the world. The combination of ethanol and caffeine ingested by consuming energy drinks is becoming increasingly popular among young people. We analyzed the effect of simultaneous consumption of ethanol and caffeine on the serum profile of miRNAs differentially expressed in the ethanol-drinking rat model (UChB strain). Adult rats were divided into three groups (n = 5 per group): UChB group (rats fed with 1 : 10 (v/v) ethanol ad libitum); UChB + caffeine group (rats fed with 1 : 10 (v/v) ethanol ad libitum + 3 g L-1 of caffeine); control group (rats drinking water used as the control for UChB). The treatment with caffeine occurred from day 95 to 150 days old, totalizing 55 days of ethanol + caffeine ingestion. The expressions of microRNAs (miR) -9-3p, -15b-5p, -16-5p, -21-5p, -200a-3p and -222-3p were detected by Real Time-PCR (RT-PCR). The expressions of miR-9-3p, -15b-5p, -16-5p and -222-3p were upregulated in the UChB group. Conversely, simultaneous ingestion of ethanol and caffeine significantly reversed these expressions to similar levels to control animals, thus emphasizing that caffeine had a protective effect in the presence of ethanol. In addition, miR-21-5p was downregulated with ethanol consumption whereas miR-222-3p was unchanged. Ethanol and caffeine consumption was capable of altering serum miRNAs, which are potential biomarkers for the systemic effects of these addictive substances. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Serum miRNAs are differentially altered by ethanol and caffeine consumption in ratsAlcoholism is a multifactorial disease with high risk for dependence determined by genetic background, environmental factors and neuroadaptations. The excessive consumption of this substance is related to psychiatric problems, epilepsy, cardiovascular disease, cirrhosis and cancers. Caffeine is one of the most popular psychostimulants currently consumed in the world. The combination of ethanol and caffeine ingested by consuming energy drinks is becoming increasingly popular among young people. We analyzed the effect of simultaneous consumption of ethanol and caffeine on the serum profile of miRNAs differentially expressed in the ethanol-drinking rat model (UChB strain). Adult rats were divided into three groups (n = 5 per group): UChB group (rats fed with 1 : 10 (v/v) ethanol ad libitum); UChB + caffeine group (rats fed with 1 : 10 (v/v) ethanol ad libitum + 3 g L-1 of caffeine); control group (rats drinking water used as the control for UChB). The treatment with caffeine occurred from day 95 to 150 days old, totalizing 55 days of ethanol + caffeine ingestion. The expressions of microRNAs (miR) -9-3p, -15b-5p, -16-5p, -21-5p, -200a-3p and -222-3p were detected by Real Time-PCR (RT-PCR). The expressions of miR-9-3p, -15b-5p, -16-5p and -222-3p were upregulated in the UChB group. Conversely, simultaneous ingestion of ethanol and caffeine significantly reversed these expressions to similar levels to control animals, thus emphasizing that caffeine had a protective effect in the presence of ethanol. In addition, miR-21-5p was downregulated with ethanol consumption whereas miR-222-3p was unchanged. Ethanol and caffeine consumption was capable of altering serum miRNAs, which are potential biomarkers for the systemic effects of these addictive substances.Department of Morphology and Pathology Federal University of São Carlos (UFSCar)Department Structural and Functional Biology University of Campinas (UNICAMP)Department of Surgery and Anatomy University of São Paulo (USP)Department of Anatomy State University of São Paulo (UNESP)Department of Anatomy State University of São Paulo (UNESP)Universidade Estadual Paulista (Unesp)Martinez, M.Rossetto, I. M.U.Arantes, R. M.S.Lizarte, F. S.N.Tirapelli, L. F.Tirapelli, D. P.C.Chuffa, L. G.A. [UNESP]Martinez, F. E. [UNESP]2020-12-12T01:08:23Z2020-12-12T01:08:23Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article842-849http://dx.doi.org/10.1039/c9tx00069kToxicology Research, v. 8, n. 6, p. 842-849, 2019.2045-45382045-452Xhttp://hdl.handle.net/11449/19827710.1039/c9tx00069k2-s2.0-85076484406Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxicology Researchinfo:eu-repo/semantics/openAccess2021-10-23T10:11:21Zoai:repositorio.unesp.br:11449/198277Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:14:20.324532Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats |
title |
Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats |
spellingShingle |
Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats Martinez, M. |
title_short |
Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats |
title_full |
Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats |
title_fullStr |
Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats |
title_full_unstemmed |
Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats |
title_sort |
Serum miRNAs are differentially altered by ethanol and caffeine consumption in rats |
author |
Martinez, M. |
author_facet |
Martinez, M. Rossetto, I. M.U. Arantes, R. M.S. Lizarte, F. S.N. Tirapelli, L. F. Tirapelli, D. P.C. Chuffa, L. G.A. [UNESP] Martinez, F. E. [UNESP] |
author_role |
author |
author2 |
Rossetto, I. M.U. Arantes, R. M.S. Lizarte, F. S.N. Tirapelli, L. F. Tirapelli, D. P.C. Chuffa, L. G.A. [UNESP] Martinez, F. E. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Martinez, M. Rossetto, I. M.U. Arantes, R. M.S. Lizarte, F. S.N. Tirapelli, L. F. Tirapelli, D. P.C. Chuffa, L. G.A. [UNESP] Martinez, F. E. [UNESP] |
description |
Alcoholism is a multifactorial disease with high risk for dependence determined by genetic background, environmental factors and neuroadaptations. The excessive consumption of this substance is related to psychiatric problems, epilepsy, cardiovascular disease, cirrhosis and cancers. Caffeine is one of the most popular psychostimulants currently consumed in the world. The combination of ethanol and caffeine ingested by consuming energy drinks is becoming increasingly popular among young people. We analyzed the effect of simultaneous consumption of ethanol and caffeine on the serum profile of miRNAs differentially expressed in the ethanol-drinking rat model (UChB strain). Adult rats were divided into three groups (n = 5 per group): UChB group (rats fed with 1 : 10 (v/v) ethanol ad libitum); UChB + caffeine group (rats fed with 1 : 10 (v/v) ethanol ad libitum + 3 g L-1 of caffeine); control group (rats drinking water used as the control for UChB). The treatment with caffeine occurred from day 95 to 150 days old, totalizing 55 days of ethanol + caffeine ingestion. The expressions of microRNAs (miR) -9-3p, -15b-5p, -16-5p, -21-5p, -200a-3p and -222-3p were detected by Real Time-PCR (RT-PCR). The expressions of miR-9-3p, -15b-5p, -16-5p and -222-3p were upregulated in the UChB group. Conversely, simultaneous ingestion of ethanol and caffeine significantly reversed these expressions to similar levels to control animals, thus emphasizing that caffeine had a protective effect in the presence of ethanol. In addition, miR-21-5p was downregulated with ethanol consumption whereas miR-222-3p was unchanged. Ethanol and caffeine consumption was capable of altering serum miRNAs, which are potential biomarkers for the systemic effects of these addictive substances. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 2020-12-12T01:08:23Z 2020-12-12T01:08:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1039/c9tx00069k Toxicology Research, v. 8, n. 6, p. 842-849, 2019. 2045-4538 2045-452X http://hdl.handle.net/11449/198277 10.1039/c9tx00069k 2-s2.0-85076484406 |
url |
http://dx.doi.org/10.1039/c9tx00069k http://hdl.handle.net/11449/198277 |
identifier_str_mv |
Toxicology Research, v. 8, n. 6, p. 842-849, 2019. 2045-4538 2045-452X 10.1039/c9tx00069k 2-s2.0-85076484406 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicology Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
842-849 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129598718738432 |