Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats

Detalhes bibliográficos
Autor(a) principal: Rossetto, I. M.U.
Data de Publicação: 2019
Outros Autores: Cagnon, V. H.A., Lizarte, F. S.N., Tirapelli, L. F., Tirapelli, D. P.C., Arantes, R. M.S., Chuffa, L. G.A. [UNESP], Martinez, F. E. [UNESP], Martinez, M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.lfs.2019.05.016
http://hdl.handle.net/11449/187678
Resumo: Aims: The present study aimed to verify changes in cerebellar and plasmatic expression of miRNAs after the chronic consumption of ethanol and caffeine in the UChB rat, an experimental model for alcoholism. Material and methods: Male rats at 5 months of age, were divided into the following groups (n = 10/group): 1. Ethanol (UChB rats receiving 10% ethanol solution and water ad libitum); 2. Ethanol + caffeine (UChB rats receiving 10% ethanol solution + 3g/l caffeine and water ad libitum); 3. Control (rats receiving water ad libitum). The cerebellum and plasma of the animals were collected and processed by RT-PCR for the miRNAs-155-5p, -146a-5p, -126-3p, -132-3p, -339-5p. Key findings: Ethanol and caffeine were capable of regulating the expression of miRNAs associated with the inflammatory process in the tissue and plasma of the UChB rats. Increased expression of the analyzed miRNAs-155-5p, -146a-5p, -126-3p, -132-3p was observed for the cerebellar tissue in the Ethanol group and reduced expression of them in the Ethanol + caffeine group. In plasma, caffeine significantly elevated the miR-126-3p and miR-132-3p levels and decreased miR-155-5p levels. Ethanol consumption increased miR-146a-5p expression and decreased miR-339-5p levels. In brief, altered plasmatic levels of the miRNAs did not reflect the miRNAs levels found in cerebellar tissue. Significance: Considering the results herein, we concluded that ethanol predisposes to an inflammatory process while caffeine has a neuroprotective effect on the cerebellar tissue.
id UNSP_7fd28b25f35bf57f39d453b04f2c8da3
oai_identifier_str oai:repositorio.unesp.br:11449/187678
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB ratsCaffeineCerebellumEthanolmiRNAPlasmaUChB ratsAims: The present study aimed to verify changes in cerebellar and plasmatic expression of miRNAs after the chronic consumption of ethanol and caffeine in the UChB rat, an experimental model for alcoholism. Material and methods: Male rats at 5 months of age, were divided into the following groups (n = 10/group): 1. Ethanol (UChB rats receiving 10% ethanol solution and water ad libitum); 2. Ethanol + caffeine (UChB rats receiving 10% ethanol solution + 3g/l caffeine and water ad libitum); 3. Control (rats receiving water ad libitum). The cerebellum and plasma of the animals were collected and processed by RT-PCR for the miRNAs-155-5p, -146a-5p, -126-3p, -132-3p, -339-5p. Key findings: Ethanol and caffeine were capable of regulating the expression of miRNAs associated with the inflammatory process in the tissue and plasma of the UChB rats. Increased expression of the analyzed miRNAs-155-5p, -146a-5p, -126-3p, -132-3p was observed for the cerebellar tissue in the Ethanol group and reduced expression of them in the Ethanol + caffeine group. In plasma, caffeine significantly elevated the miR-126-3p and miR-132-3p levels and decreased miR-155-5p levels. Ethanol consumption increased miR-146a-5p expression and decreased miR-339-5p levels. In brief, altered plasmatic levels of the miRNAs did not reflect the miRNAs levels found in cerebellar tissue. Significance: Considering the results herein, we concluded that ethanol predisposes to an inflammatory process while caffeine has a neuroprotective effect on the cerebellar tissue.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Structural and Functional Biology University of Campinas (UNICAMP)Department of Surgery and Anatomy University of São Paulo (USP)Department of Morphology e Pathology Federal University of São Carlos (UFSCar)Department of Anatomy State University of São Paulo (UNESP)Department of Anatomy State University of São Paulo (UNESP)FAPESP: 2016/07410-8Universidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Rossetto, I. M.U.Cagnon, V. H.A.Lizarte, F. S.N.Tirapelli, L. F.Tirapelli, D. P.C.Arantes, R. M.S.Chuffa, L. G.A. [UNESP]Martinez, F. E. [UNESP]Martinez, M.2019-10-06T15:43:56Z2019-10-06T15:43:56Z2019-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article180-186http://dx.doi.org/10.1016/j.lfs.2019.05.016Life Sciences, v. 229, p. 180-186.1879-06310024-3205http://hdl.handle.net/11449/18767810.1016/j.lfs.2019.05.0162-s2.0-85065872308Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLife Sciencesinfo:eu-repo/semantics/openAccess2021-10-23T11:59:59Zoai:repositorio.unesp.br:11449/187678Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:17:45.351078Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats
title Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats
spellingShingle Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats
Rossetto, I. M.U.
Caffeine
Cerebellum
Ethanol
miRNA
Plasma
UChB rats
title_short Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats
title_full Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats
title_fullStr Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats
title_full_unstemmed Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats
title_sort Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats
author Rossetto, I. M.U.
author_facet Rossetto, I. M.U.
Cagnon, V. H.A.
Lizarte, F. S.N.
Tirapelli, L. F.
Tirapelli, D. P.C.
Arantes, R. M.S.
Chuffa, L. G.A. [UNESP]
Martinez, F. E. [UNESP]
Martinez, M.
author_role author
author2 Cagnon, V. H.A.
Lizarte, F. S.N.
Tirapelli, L. F.
Tirapelli, D. P.C.
Arantes, R. M.S.
Chuffa, L. G.A. [UNESP]
Martinez, F. E. [UNESP]
Martinez, M.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Universidade Federal de São Carlos (UFSCar)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Rossetto, I. M.U.
Cagnon, V. H.A.
Lizarte, F. S.N.
Tirapelli, L. F.
Tirapelli, D. P.C.
Arantes, R. M.S.
Chuffa, L. G.A. [UNESP]
Martinez, F. E. [UNESP]
Martinez, M.
dc.subject.por.fl_str_mv Caffeine
Cerebellum
Ethanol
miRNA
Plasma
UChB rats
topic Caffeine
Cerebellum
Ethanol
miRNA
Plasma
UChB rats
description Aims: The present study aimed to verify changes in cerebellar and plasmatic expression of miRNAs after the chronic consumption of ethanol and caffeine in the UChB rat, an experimental model for alcoholism. Material and methods: Male rats at 5 months of age, were divided into the following groups (n = 10/group): 1. Ethanol (UChB rats receiving 10% ethanol solution and water ad libitum); 2. Ethanol + caffeine (UChB rats receiving 10% ethanol solution + 3g/l caffeine and water ad libitum); 3. Control (rats receiving water ad libitum). The cerebellum and plasma of the animals were collected and processed by RT-PCR for the miRNAs-155-5p, -146a-5p, -126-3p, -132-3p, -339-5p. Key findings: Ethanol and caffeine were capable of regulating the expression of miRNAs associated with the inflammatory process in the tissue and plasma of the UChB rats. Increased expression of the analyzed miRNAs-155-5p, -146a-5p, -126-3p, -132-3p was observed for the cerebellar tissue in the Ethanol group and reduced expression of them in the Ethanol + caffeine group. In plasma, caffeine significantly elevated the miR-126-3p and miR-132-3p levels and decreased miR-155-5p levels. Ethanol consumption increased miR-146a-5p expression and decreased miR-339-5p levels. In brief, altered plasmatic levels of the miRNAs did not reflect the miRNAs levels found in cerebellar tissue. Significance: Considering the results herein, we concluded that ethanol predisposes to an inflammatory process while caffeine has a neuroprotective effect on the cerebellar tissue.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T15:43:56Z
2019-10-06T15:43:56Z
2019-07-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.lfs.2019.05.016
Life Sciences, v. 229, p. 180-186.
1879-0631
0024-3205
http://hdl.handle.net/11449/187678
10.1016/j.lfs.2019.05.016
2-s2.0-85065872308
url http://dx.doi.org/10.1016/j.lfs.2019.05.016
http://hdl.handle.net/11449/187678
identifier_str_mv Life Sciences, v. 229, p. 180-186.
1879-0631
0024-3205
10.1016/j.lfs.2019.05.016
2-s2.0-85065872308
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Life Sciences
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 180-186
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128342155591680