Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.lfs.2019.05.016 http://hdl.handle.net/11449/187678 |
Resumo: | Aims: The present study aimed to verify changes in cerebellar and plasmatic expression of miRNAs after the chronic consumption of ethanol and caffeine in the UChB rat, an experimental model for alcoholism. Material and methods: Male rats at 5 months of age, were divided into the following groups (n = 10/group): 1. Ethanol (UChB rats receiving 10% ethanol solution and water ad libitum); 2. Ethanol + caffeine (UChB rats receiving 10% ethanol solution + 3g/l caffeine and water ad libitum); 3. Control (rats receiving water ad libitum). The cerebellum and plasma of the animals were collected and processed by RT-PCR for the miRNAs-155-5p, -146a-5p, -126-3p, -132-3p, -339-5p. Key findings: Ethanol and caffeine were capable of regulating the expression of miRNAs associated with the inflammatory process in the tissue and plasma of the UChB rats. Increased expression of the analyzed miRNAs-155-5p, -146a-5p, -126-3p, -132-3p was observed for the cerebellar tissue in the Ethanol group and reduced expression of them in the Ethanol + caffeine group. In plasma, caffeine significantly elevated the miR-126-3p and miR-132-3p levels and decreased miR-155-5p levels. Ethanol consumption increased miR-146a-5p expression and decreased miR-339-5p levels. In brief, altered plasmatic levels of the miRNAs did not reflect the miRNAs levels found in cerebellar tissue. Significance: Considering the results herein, we concluded that ethanol predisposes to an inflammatory process while caffeine has a neuroprotective effect on the cerebellar tissue. |
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Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB ratsCaffeineCerebellumEthanolmiRNAPlasmaUChB ratsAims: The present study aimed to verify changes in cerebellar and plasmatic expression of miRNAs after the chronic consumption of ethanol and caffeine in the UChB rat, an experimental model for alcoholism. Material and methods: Male rats at 5 months of age, were divided into the following groups (n = 10/group): 1. Ethanol (UChB rats receiving 10% ethanol solution and water ad libitum); 2. Ethanol + caffeine (UChB rats receiving 10% ethanol solution + 3g/l caffeine and water ad libitum); 3. Control (rats receiving water ad libitum). The cerebellum and plasma of the animals were collected and processed by RT-PCR for the miRNAs-155-5p, -146a-5p, -126-3p, -132-3p, -339-5p. Key findings: Ethanol and caffeine were capable of regulating the expression of miRNAs associated with the inflammatory process in the tissue and plasma of the UChB rats. Increased expression of the analyzed miRNAs-155-5p, -146a-5p, -126-3p, -132-3p was observed for the cerebellar tissue in the Ethanol group and reduced expression of them in the Ethanol + caffeine group. In plasma, caffeine significantly elevated the miR-126-3p and miR-132-3p levels and decreased miR-155-5p levels. Ethanol consumption increased miR-146a-5p expression and decreased miR-339-5p levels. In brief, altered plasmatic levels of the miRNAs did not reflect the miRNAs levels found in cerebellar tissue. Significance: Considering the results herein, we concluded that ethanol predisposes to an inflammatory process while caffeine has a neuroprotective effect on the cerebellar tissue.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Structural and Functional Biology University of Campinas (UNICAMP)Department of Surgery and Anatomy University of São Paulo (USP)Department of Morphology e Pathology Federal University of São Carlos (UFSCar)Department of Anatomy State University of São Paulo (UNESP)Department of Anatomy State University of São Paulo (UNESP)FAPESP: 2016/07410-8Universidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Rossetto, I. M.U.Cagnon, V. H.A.Lizarte, F. S.N.Tirapelli, L. F.Tirapelli, D. P.C.Arantes, R. M.S.Chuffa, L. G.A. [UNESP]Martinez, F. E. [UNESP]Martinez, M.2019-10-06T15:43:56Z2019-10-06T15:43:56Z2019-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article180-186http://dx.doi.org/10.1016/j.lfs.2019.05.016Life Sciences, v. 229, p. 180-186.1879-06310024-3205http://hdl.handle.net/11449/18767810.1016/j.lfs.2019.05.0162-s2.0-85065872308Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLife Sciencesinfo:eu-repo/semantics/openAccess2021-10-23T11:59:59Zoai:repositorio.unesp.br:11449/187678Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:17:45.351078Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats |
title |
Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats |
spellingShingle |
Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats Rossetto, I. M.U. Caffeine Cerebellum Ethanol miRNA Plasma UChB rats |
title_short |
Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats |
title_full |
Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats |
title_fullStr |
Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats |
title_full_unstemmed |
Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats |
title_sort |
Ethanol and caffeine consumption modulates the expression of miRNAs in the cerebellum and plasma of UChB rats |
author |
Rossetto, I. M.U. |
author_facet |
Rossetto, I. M.U. Cagnon, V. H.A. Lizarte, F. S.N. Tirapelli, L. F. Tirapelli, D. P.C. Arantes, R. M.S. Chuffa, L. G.A. [UNESP] Martinez, F. E. [UNESP] Martinez, M. |
author_role |
author |
author2 |
Cagnon, V. H.A. Lizarte, F. S.N. Tirapelli, L. F. Tirapelli, D. P.C. Arantes, R. M.S. Chuffa, L. G.A. [UNESP] Martinez, F. E. [UNESP] Martinez, M. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade de São Paulo (USP) Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rossetto, I. M.U. Cagnon, V. H.A. Lizarte, F. S.N. Tirapelli, L. F. Tirapelli, D. P.C. Arantes, R. M.S. Chuffa, L. G.A. [UNESP] Martinez, F. E. [UNESP] Martinez, M. |
dc.subject.por.fl_str_mv |
Caffeine Cerebellum Ethanol miRNA Plasma UChB rats |
topic |
Caffeine Cerebellum Ethanol miRNA Plasma UChB rats |
description |
Aims: The present study aimed to verify changes in cerebellar and plasmatic expression of miRNAs after the chronic consumption of ethanol and caffeine in the UChB rat, an experimental model for alcoholism. Material and methods: Male rats at 5 months of age, were divided into the following groups (n = 10/group): 1. Ethanol (UChB rats receiving 10% ethanol solution and water ad libitum); 2. Ethanol + caffeine (UChB rats receiving 10% ethanol solution + 3g/l caffeine and water ad libitum); 3. Control (rats receiving water ad libitum). The cerebellum and plasma of the animals were collected and processed by RT-PCR for the miRNAs-155-5p, -146a-5p, -126-3p, -132-3p, -339-5p. Key findings: Ethanol and caffeine were capable of regulating the expression of miRNAs associated with the inflammatory process in the tissue and plasma of the UChB rats. Increased expression of the analyzed miRNAs-155-5p, -146a-5p, -126-3p, -132-3p was observed for the cerebellar tissue in the Ethanol group and reduced expression of them in the Ethanol + caffeine group. In plasma, caffeine significantly elevated the miR-126-3p and miR-132-3p levels and decreased miR-155-5p levels. Ethanol consumption increased miR-146a-5p expression and decreased miR-339-5p levels. In brief, altered plasmatic levels of the miRNAs did not reflect the miRNAs levels found in cerebellar tissue. Significance: Considering the results herein, we concluded that ethanol predisposes to an inflammatory process while caffeine has a neuroprotective effect on the cerebellar tissue. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:43:56Z 2019-10-06T15:43:56Z 2019-07-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.lfs.2019.05.016 Life Sciences, v. 229, p. 180-186. 1879-0631 0024-3205 http://hdl.handle.net/11449/187678 10.1016/j.lfs.2019.05.016 2-s2.0-85065872308 |
url |
http://dx.doi.org/10.1016/j.lfs.2019.05.016 http://hdl.handle.net/11449/187678 |
identifier_str_mv |
Life Sciences, v. 229, p. 180-186. 1879-0631 0024-3205 10.1016/j.lfs.2019.05.016 2-s2.0-85065872308 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Life Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
180-186 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128342155591680 |