Isolation and identification of black yeasts by enrichment on atmospheres of monoaromatic hydrocarbons
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00248-010-9651-4 http://hdl.handle.net/11449/71608 |
Resumo: | Black yeast members of the Herpotrichiellaceae present a complex ecological behavior: They are often isolated from rather extreme environments polluted with aromatic hydrocarbons, while they are also regularly involved in human opportunistic infections. A selective technique to promote the in vitro growth of herpotrichiellaceous fungi was applied to investigate their ecophysiology. Samples from natural ecological niches and man-made environments that might contain black yeasts were enriched on an inert solid support at low humidity and under a controlled atmosphere rich in volatile aromatic hydrocarbons. Benzene, toluene, and xylene were provided separately as the sole carbon and energy source via the gas phase. The assayed isolation protocol was highly specific toward mesophilic Exophiala species (70 strains of this genus out of 71 isolates). Those were obtained predominantly from creosote-treated railway ties (53 strains), but isolates were also found on wild berries (11 strains) and in guano-rich soil samples (six strains). Most of the isolates were obtained on toluene (43 strains), but enrichments on xylene and benzene also yielded herpotrichiellaceous fungi (17 and 10 isolates, respectively). Based upon morphological characterizations and DNA sequences of the full internal transcriber spacers (ITS) and the 8.5S rRNA genes, the majority of the obtained isolates were affiliated to the recently described species Exophiala xenobiotica (32 strains) and Exophiala bergeri (nine strains). Members of two other phylogenetic groups (24 and two strains, respectively) somewhat related to E. bergeri were also found, and a last group (three strains) corresponded to an undescribed Exophiala species. © 2010 The Author(s). |
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Isolation and identification of black yeasts by enrichment on atmospheres of monoaromatic hydrocarbonsaromatic hydrocarbonfungal DNAribosomal spacer DNAribosome RNAclassificationculture mediumDNA sequencegeneticsisolation and purificationmetabolismmicrobiologyphylogenyyeastCulture MediaDNA, FungalDNA, Ribosomal SpacerEnvironmental MicrobiologyHydrocarbons, AromaticPhylogenyRNA, RibosomalSequence Analysis, DNAYeastsChaetothyrialesExophialaExophiala bergeriFungiHerpotrichiellaceaeBlack yeast members of the Herpotrichiellaceae present a complex ecological behavior: They are often isolated from rather extreme environments polluted with aromatic hydrocarbons, while they are also regularly involved in human opportunistic infections. A selective technique to promote the in vitro growth of herpotrichiellaceous fungi was applied to investigate their ecophysiology. Samples from natural ecological niches and man-made environments that might contain black yeasts were enriched on an inert solid support at low humidity and under a controlled atmosphere rich in volatile aromatic hydrocarbons. Benzene, toluene, and xylene were provided separately as the sole carbon and energy source via the gas phase. The assayed isolation protocol was highly specific toward mesophilic Exophiala species (70 strains of this genus out of 71 isolates). Those were obtained predominantly from creosote-treated railway ties (53 strains), but isolates were also found on wild berries (11 strains) and in guano-rich soil samples (six strains). Most of the isolates were obtained on toluene (43 strains), but enrichments on xylene and benzene also yielded herpotrichiellaceous fungi (17 and 10 isolates, respectively). Based upon morphological characterizations and DNA sequences of the full internal transcriber spacers (ITS) and the 8.5S rRNA genes, the majority of the obtained isolates were affiliated to the recently described species Exophiala xenobiotica (32 strains) and Exophiala bergeri (nine strains). Members of two other phylogenetic groups (24 and two strains, respectively) somewhat related to E. bergeri were also found, and a last group (three strains) corresponded to an undescribed Exophiala species. © 2010 The Author(s).Department of Dermatology Fujian Medical University Affiliated Union Hospital, FuzhouDepartment of Dermatology and Venereology, Union Hospital, Tongji Medical College Huazhong Science and Technology University, Jiefang Dadao, Wuhan, HubeiCBS-KNAW Fungal Biodiversity Centre, UtrechtInstitute of Biodiversity and Ecosystem Dynamics University of Amsterdam, AmsterdamResearch Center for Medical Mycology Peking University Health Science Center, BeijingUNESP Department of Biochemistry and Microbiology Institute of Biosciences, Rio Claro, SPGIRO Technological Centre, Pompeu Fabra 1, 08100 Mollet del Vallès, Barcelona, CataloniaIRTA, BarcelonaUNESP Department of Biochemistry and Microbiology Institute of Biosciences, Rio Claro, SPFujian Medical University Affiliated Union HospitalHuazhong Science and Technology UniversityCBS-KNAW Fungal Biodiversity CentreUniversity of AmsterdamPeking University Health Science CenterUniversidade Estadual Paulista (Unesp)GIRO Technological CentreIRTAZhao, JingjunZeng, Jingside Hoog, G. SybrenAttili-Angelis, Derlene [UNESP]Prenafeta-Boldú, Francesc X.2014-05-27T11:24:39Z2014-05-27T11:24:39Z2010-03-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article149-156application/pdfhttp://dx.doi.org/10.1007/s00248-010-9651-4Microbial Ecology, v. 60, n. 1, p. 149-156, 2010.0095-3628http://hdl.handle.net/11449/7160810.1007/s00248-010-9651-42-s2.0-779554173882-s2.0-77955417388.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Ecology3.6141,272info:eu-repo/semantics/openAccess2023-10-31T06:11:26Zoai:repositorio.unesp.br:11449/71608Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:32:47.653745Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Isolation and identification of black yeasts by enrichment on atmospheres of monoaromatic hydrocarbons |
title |
Isolation and identification of black yeasts by enrichment on atmospheres of monoaromatic hydrocarbons |
spellingShingle |
Isolation and identification of black yeasts by enrichment on atmospheres of monoaromatic hydrocarbons Zhao, Jingjun aromatic hydrocarbon fungal DNA ribosomal spacer DNA ribosome RNA classification culture medium DNA sequence genetics isolation and purification metabolism microbiology phylogeny yeast Culture Media DNA, Fungal DNA, Ribosomal Spacer Environmental Microbiology Hydrocarbons, Aromatic Phylogeny RNA, Ribosomal Sequence Analysis, DNA Yeasts Chaetothyriales Exophiala Exophiala bergeri Fungi Herpotrichiellaceae |
title_short |
Isolation and identification of black yeasts by enrichment on atmospheres of monoaromatic hydrocarbons |
title_full |
Isolation and identification of black yeasts by enrichment on atmospheres of monoaromatic hydrocarbons |
title_fullStr |
Isolation and identification of black yeasts by enrichment on atmospheres of monoaromatic hydrocarbons |
title_full_unstemmed |
Isolation and identification of black yeasts by enrichment on atmospheres of monoaromatic hydrocarbons |
title_sort |
Isolation and identification of black yeasts by enrichment on atmospheres of monoaromatic hydrocarbons |
author |
Zhao, Jingjun |
author_facet |
Zhao, Jingjun Zeng, Jingsi de Hoog, G. Sybren Attili-Angelis, Derlene [UNESP] Prenafeta-Boldú, Francesc X. |
author_role |
author |
author2 |
Zeng, Jingsi de Hoog, G. Sybren Attili-Angelis, Derlene [UNESP] Prenafeta-Boldú, Francesc X. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Fujian Medical University Affiliated Union Hospital Huazhong Science and Technology University CBS-KNAW Fungal Biodiversity Centre University of Amsterdam Peking University Health Science Center Universidade Estadual Paulista (Unesp) GIRO Technological Centre IRTA |
dc.contributor.author.fl_str_mv |
Zhao, Jingjun Zeng, Jingsi de Hoog, G. Sybren Attili-Angelis, Derlene [UNESP] Prenafeta-Boldú, Francesc X. |
dc.subject.por.fl_str_mv |
aromatic hydrocarbon fungal DNA ribosomal spacer DNA ribosome RNA classification culture medium DNA sequence genetics isolation and purification metabolism microbiology phylogeny yeast Culture Media DNA, Fungal DNA, Ribosomal Spacer Environmental Microbiology Hydrocarbons, Aromatic Phylogeny RNA, Ribosomal Sequence Analysis, DNA Yeasts Chaetothyriales Exophiala Exophiala bergeri Fungi Herpotrichiellaceae |
topic |
aromatic hydrocarbon fungal DNA ribosomal spacer DNA ribosome RNA classification culture medium DNA sequence genetics isolation and purification metabolism microbiology phylogeny yeast Culture Media DNA, Fungal DNA, Ribosomal Spacer Environmental Microbiology Hydrocarbons, Aromatic Phylogeny RNA, Ribosomal Sequence Analysis, DNA Yeasts Chaetothyriales Exophiala Exophiala bergeri Fungi Herpotrichiellaceae |
description |
Black yeast members of the Herpotrichiellaceae present a complex ecological behavior: They are often isolated from rather extreme environments polluted with aromatic hydrocarbons, while they are also regularly involved in human opportunistic infections. A selective technique to promote the in vitro growth of herpotrichiellaceous fungi was applied to investigate their ecophysiology. Samples from natural ecological niches and man-made environments that might contain black yeasts were enriched on an inert solid support at low humidity and under a controlled atmosphere rich in volatile aromatic hydrocarbons. Benzene, toluene, and xylene were provided separately as the sole carbon and energy source via the gas phase. The assayed isolation protocol was highly specific toward mesophilic Exophiala species (70 strains of this genus out of 71 isolates). Those were obtained predominantly from creosote-treated railway ties (53 strains), but isolates were also found on wild berries (11 strains) and in guano-rich soil samples (six strains). Most of the isolates were obtained on toluene (43 strains), but enrichments on xylene and benzene also yielded herpotrichiellaceous fungi (17 and 10 isolates, respectively). Based upon morphological characterizations and DNA sequences of the full internal transcriber spacers (ITS) and the 8.5S rRNA genes, the majority of the obtained isolates were affiliated to the recently described species Exophiala xenobiotica (32 strains) and Exophiala bergeri (nine strains). Members of two other phylogenetic groups (24 and two strains, respectively) somewhat related to E. bergeri were also found, and a last group (three strains) corresponded to an undescribed Exophiala species. © 2010 The Author(s). |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-03-23 2014-05-27T11:24:39Z 2014-05-27T11:24:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00248-010-9651-4 Microbial Ecology, v. 60, n. 1, p. 149-156, 2010. 0095-3628 http://hdl.handle.net/11449/71608 10.1007/s00248-010-9651-4 2-s2.0-77955417388 2-s2.0-77955417388.pdf |
url |
http://dx.doi.org/10.1007/s00248-010-9651-4 http://hdl.handle.net/11449/71608 |
identifier_str_mv |
Microbial Ecology, v. 60, n. 1, p. 149-156, 2010. 0095-3628 10.1007/s00248-010-9651-4 2-s2.0-77955417388 2-s2.0-77955417388.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microbial Ecology 3.614 1,272 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
149-156 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128669264117760 |