Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples

Detalhes bibliográficos
Autor(a) principal: Rocha, Giovanna S. [UNESP]
Data de Publicação: 2020
Outros Autores: Silva, Martin K. L. [UNESP], Cesarino, Ivana [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/ma13071751
http://hdl.handle.net/11449/198866
Resumo: A simple, cheap, and less aggressive immobilization procedure for biomolecules using reduced graphene oxide (rGO) was employed to prepare an impedimetric immunosensor for detection of staphylococcal enterotoxin A (SEA) from Staphylococcus aureus in milk samples. The scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS) were used to monitor the single steps of the electrode assembly process. The glassy carbon (GC)/rGO platform detected the antigen-antibody binding procedures of SEA with concentrations of 0.5 to 3.5 mg L-1 via impedance changes in a low frequency range. The impedimetric immunosensor was successfully applied for the determination of SEA in milk samples.
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spelling Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk SamplesEnterotoxinAImpedimetric immunosensorReduced graphene oxideStaphylococcus aureusA simple, cheap, and less aggressive immobilization procedure for biomolecules using reduced graphene oxide (rGO) was employed to prepare an impedimetric immunosensor for detection of staphylococcal enterotoxin A (SEA) from Staphylococcus aureus in milk samples. The scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS) were used to monitor the single steps of the electrode assembly process. The glassy carbon (GC)/rGO platform detected the antigen-antibody binding procedures of SEA with concentrations of 0.5 to 3.5 mg L-1 via impedance changes in a low frequency range. The impedimetric immunosensor was successfully applied for the determination of SEA in milk samples.Department of Bioprocesses and Biotechnology School of Agriculture São Paulo State University (UNESP)Department of Bioprocesses and Biotechnology School of Agriculture São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Rocha, Giovanna S. [UNESP]Silva, Martin K. L. [UNESP]Cesarino, Ivana [UNESP]2020-12-12T01:24:05Z2020-12-12T01:24:05Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ma13071751Materials, v. 13, n. 7, 2020.1996-1944http://hdl.handle.net/11449/19886610.3390/ma130717512-s2.0-85085087784Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterialsinfo:eu-repo/semantics/openAccess2021-10-22T20:42:55Zoai:repositorio.unesp.br:11449/198866Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T20:42:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples
title Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples
spellingShingle Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples
Rocha, Giovanna S. [UNESP]
EnterotoxinA
Impedimetric immunosensor
Reduced graphene oxide
Staphylococcus aureus
title_short Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples
title_full Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples
title_fullStr Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples
title_full_unstemmed Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples
title_sort Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples
author Rocha, Giovanna S. [UNESP]
author_facet Rocha, Giovanna S. [UNESP]
Silva, Martin K. L. [UNESP]
Cesarino, Ivana [UNESP]
author_role author
author2 Silva, Martin K. L. [UNESP]
Cesarino, Ivana [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Rocha, Giovanna S. [UNESP]
Silva, Martin K. L. [UNESP]
Cesarino, Ivana [UNESP]
dc.subject.por.fl_str_mv EnterotoxinA
Impedimetric immunosensor
Reduced graphene oxide
Staphylococcus aureus
topic EnterotoxinA
Impedimetric immunosensor
Reduced graphene oxide
Staphylococcus aureus
description A simple, cheap, and less aggressive immobilization procedure for biomolecules using reduced graphene oxide (rGO) was employed to prepare an impedimetric immunosensor for detection of staphylococcal enterotoxin A (SEA) from Staphylococcus aureus in milk samples. The scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS) were used to monitor the single steps of the electrode assembly process. The glassy carbon (GC)/rGO platform detected the antigen-antibody binding procedures of SEA with concentrations of 0.5 to 3.5 mg L-1 via impedance changes in a low frequency range. The impedimetric immunosensor was successfully applied for the determination of SEA in milk samples.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:24:05Z
2020-12-12T01:24:05Z
2020-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/ma13071751
Materials, v. 13, n. 7, 2020.
1996-1944
http://hdl.handle.net/11449/198866
10.3390/ma13071751
2-s2.0-85085087784
url http://dx.doi.org/10.3390/ma13071751
http://hdl.handle.net/11449/198866
identifier_str_mv Materials, v. 13, n. 7, 2020.
1996-1944
10.3390/ma13071751
2-s2.0-85085087784
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Materials
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799964407527112704

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