Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ma13071751 http://hdl.handle.net/11449/198866 |
Resumo: | A simple, cheap, and less aggressive immobilization procedure for biomolecules using reduced graphene oxide (rGO) was employed to prepare an impedimetric immunosensor for detection of staphylococcal enterotoxin A (SEA) from Staphylococcus aureus in milk samples. The scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS) were used to monitor the single steps of the electrode assembly process. The glassy carbon (GC)/rGO platform detected the antigen-antibody binding procedures of SEA with concentrations of 0.5 to 3.5 mg L-1 via impedance changes in a low frequency range. The impedimetric immunosensor was successfully applied for the determination of SEA in milk samples. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk SamplesEnterotoxinAImpedimetric immunosensorReduced graphene oxideStaphylococcus aureusA simple, cheap, and less aggressive immobilization procedure for biomolecules using reduced graphene oxide (rGO) was employed to prepare an impedimetric immunosensor for detection of staphylococcal enterotoxin A (SEA) from Staphylococcus aureus in milk samples. The scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS) were used to monitor the single steps of the electrode assembly process. The glassy carbon (GC)/rGO platform detected the antigen-antibody binding procedures of SEA with concentrations of 0.5 to 3.5 mg L-1 via impedance changes in a low frequency range. The impedimetric immunosensor was successfully applied for the determination of SEA in milk samples.Department of Bioprocesses and Biotechnology School of Agriculture São Paulo State University (UNESP)Department of Bioprocesses and Biotechnology School of Agriculture São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Rocha, Giovanna S. [UNESP]Silva, Martin K. L. [UNESP]Cesarino, Ivana [UNESP]2020-12-12T01:24:05Z2020-12-12T01:24:05Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ma13071751Materials, v. 13, n. 7, 2020.1996-1944http://hdl.handle.net/11449/19886610.3390/ma130717512-s2.0-85085087784Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterialsinfo:eu-repo/semantics/openAccess2021-10-22T20:42:55Zoai:repositorio.unesp.br:11449/198866Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:48:22.100907Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples |
title |
Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples |
spellingShingle |
Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples Rocha, Giovanna S. [UNESP] EnterotoxinA Impedimetric immunosensor Reduced graphene oxide Staphylococcus aureus |
title_short |
Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples |
title_full |
Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples |
title_fullStr |
Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples |
title_full_unstemmed |
Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples |
title_sort |
Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples |
author |
Rocha, Giovanna S. [UNESP] |
author_facet |
Rocha, Giovanna S. [UNESP] Silva, Martin K. L. [UNESP] Cesarino, Ivana [UNESP] |
author_role |
author |
author2 |
Silva, Martin K. L. [UNESP] Cesarino, Ivana [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rocha, Giovanna S. [UNESP] Silva, Martin K. L. [UNESP] Cesarino, Ivana [UNESP] |
dc.subject.por.fl_str_mv |
EnterotoxinA Impedimetric immunosensor Reduced graphene oxide Staphylococcus aureus |
topic |
EnterotoxinA Impedimetric immunosensor Reduced graphene oxide Staphylococcus aureus |
description |
A simple, cheap, and less aggressive immobilization procedure for biomolecules using reduced graphene oxide (rGO) was employed to prepare an impedimetric immunosensor for detection of staphylococcal enterotoxin A (SEA) from Staphylococcus aureus in milk samples. The scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS) were used to monitor the single steps of the electrode assembly process. The glassy carbon (GC)/rGO platform detected the antigen-antibody binding procedures of SEA with concentrations of 0.5 to 3.5 mg L-1 via impedance changes in a low frequency range. The impedimetric immunosensor was successfully applied for the determination of SEA in milk samples. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:24:05Z 2020-12-12T01:24:05Z 2020-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ma13071751 Materials, v. 13, n. 7, 2020. 1996-1944 http://hdl.handle.net/11449/198866 10.3390/ma13071751 2-s2.0-85085087784 |
url |
http://dx.doi.org/10.3390/ma13071751 http://hdl.handle.net/11449/198866 |
identifier_str_mv |
Materials, v. 13, n. 7, 2020. 1996-1944 10.3390/ma13071751 2-s2.0-85085087784 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128278257467392 |