Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/08927014.2018.1504926 http://hdl.handle.net/11449/189817 |
Resumo: | This study evaluated the antibacterial activity of terpinen-4-ol against Streptococcus mutans and Lactobacillus acidophilus and its influence on gbpA (S. mutans) and slpA (L. acidophilus) gene expression. As measured by XTT assay, the concentrations of terpinen-4-ol that effectively inhibited the biofilm were 0.24% and 0.95% for S. mutans and L. acidophilus, respectively. Confocal microscopy revealed the presence of a biofilm attached to the enamel and dentin block surfaces with significant terpinen-4-ol effects against these microorganisms. The expression of the gbpA and slpA genes involved in adherence and biofilm formation was investigated using RT-PCR. Expression of these genes decreased after 15 min with 0.24% and 0.95% terpinen-4-ol in S. mutans and L. acidophilus, respectively. These findings demonstrate the antimicrobial activity of terpinen-4-ol and its ability to modulate the expression of gbpA and slpA genes, emphasizing the therapeutic capacity of terpinen-4-ol as an alternative to inhibit adherence in biofilm. |
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Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expressionbiofilmsMicroorganismsterpinen-4-olThis study evaluated the antibacterial activity of terpinen-4-ol against Streptococcus mutans and Lactobacillus acidophilus and its influence on gbpA (S. mutans) and slpA (L. acidophilus) gene expression. As measured by XTT assay, the concentrations of terpinen-4-ol that effectively inhibited the biofilm were 0.24% and 0.95% for S. mutans and L. acidophilus, respectively. Confocal microscopy revealed the presence of a biofilm attached to the enamel and dentin block surfaces with significant terpinen-4-ol effects against these microorganisms. The expression of the gbpA and slpA genes involved in adherence and biofilm formation was investigated using RT-PCR. Expression of these genes decreased after 15 min with 0.24% and 0.95% terpinen-4-ol in S. mutans and L. acidophilus, respectively. These findings demonstrate the antimicrobial activity of terpinen-4-ol and its ability to modulate the expression of gbpA and slpA genes, emphasizing the therapeutic capacity of terpinen-4-ol as an alternative to inhibit adherence in biofilm.Department of Physiology and Pathology State University of Sao Paulo/UNESPDepartment of Periodontology Universidade Nove de JulhoPhysics Institute of São Carlos University of São Paulo USPDepartment of Physiology and Pathology State University of Sao Paulo/UNESPUniversidade Estadual Paulista (Unesp)Universidade Nove de JulhoUniversidade de São Paulo (USP)Bordini, Ester Alves Ferreira [UNESP]Tonon, Caroline Coradi [UNESP]Francisconi, Renata Serignoli [UNESP]Magalhães, Fernando Augusto Cintra [UNESP]Huacho, Patrícia Milagros Maquera [UNESP]Bedran, Telma LombardoPratavieira, SebastiãoSpolidorio, Luis Carlos [UNESP]Spolidorio, Denise Palomari [UNESP]2019-10-06T16:53:08Z2019-10-06T16:53:08Z2018-08-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article815-825http://dx.doi.org/10.1080/08927014.2018.1504926Biofouling, v. 34, n. 7, p. 815-825, 2018.1029-24540892-7014http://hdl.handle.net/11449/18981710.1080/08927014.2018.15049262-s2.0-850550848832640929291808415Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiofoulinginfo:eu-repo/semantics/openAccess2021-10-23T18:56:51Zoai:repositorio.unesp.br:11449/189817Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:05:52.854238Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression |
title |
Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression |
spellingShingle |
Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression Bordini, Ester Alves Ferreira [UNESP] biofilms Microorganisms terpinen-4-ol |
title_short |
Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression |
title_full |
Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression |
title_fullStr |
Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression |
title_full_unstemmed |
Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression |
title_sort |
Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression |
author |
Bordini, Ester Alves Ferreira [UNESP] |
author_facet |
Bordini, Ester Alves Ferreira [UNESP] Tonon, Caroline Coradi [UNESP] Francisconi, Renata Serignoli [UNESP] Magalhães, Fernando Augusto Cintra [UNESP] Huacho, Patrícia Milagros Maquera [UNESP] Bedran, Telma Lombardo Pratavieira, Sebastião Spolidorio, Luis Carlos [UNESP] Spolidorio, Denise Palomari [UNESP] |
author_role |
author |
author2 |
Tonon, Caroline Coradi [UNESP] Francisconi, Renata Serignoli [UNESP] Magalhães, Fernando Augusto Cintra [UNESP] Huacho, Patrícia Milagros Maquera [UNESP] Bedran, Telma Lombardo Pratavieira, Sebastião Spolidorio, Luis Carlos [UNESP] Spolidorio, Denise Palomari [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Nove de Julho Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Bordini, Ester Alves Ferreira [UNESP] Tonon, Caroline Coradi [UNESP] Francisconi, Renata Serignoli [UNESP] Magalhães, Fernando Augusto Cintra [UNESP] Huacho, Patrícia Milagros Maquera [UNESP] Bedran, Telma Lombardo Pratavieira, Sebastião Spolidorio, Luis Carlos [UNESP] Spolidorio, Denise Palomari [UNESP] |
dc.subject.por.fl_str_mv |
biofilms Microorganisms terpinen-4-ol |
topic |
biofilms Microorganisms terpinen-4-ol |
description |
This study evaluated the antibacterial activity of terpinen-4-ol against Streptococcus mutans and Lactobacillus acidophilus and its influence on gbpA (S. mutans) and slpA (L. acidophilus) gene expression. As measured by XTT assay, the concentrations of terpinen-4-ol that effectively inhibited the biofilm were 0.24% and 0.95% for S. mutans and L. acidophilus, respectively. Confocal microscopy revealed the presence of a biofilm attached to the enamel and dentin block surfaces with significant terpinen-4-ol effects against these microorganisms. The expression of the gbpA and slpA genes involved in adherence and biofilm formation was investigated using RT-PCR. Expression of these genes decreased after 15 min with 0.24% and 0.95% terpinen-4-ol in S. mutans and L. acidophilus, respectively. These findings demonstrate the antimicrobial activity of terpinen-4-ol and its ability to modulate the expression of gbpA and slpA genes, emphasizing the therapeutic capacity of terpinen-4-ol as an alternative to inhibit adherence in biofilm. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-08-09 2019-10-06T16:53:08Z 2019-10-06T16:53:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/08927014.2018.1504926 Biofouling, v. 34, n. 7, p. 815-825, 2018. 1029-2454 0892-7014 http://hdl.handle.net/11449/189817 10.1080/08927014.2018.1504926 2-s2.0-85055084883 2640929291808415 |
url |
http://dx.doi.org/10.1080/08927014.2018.1504926 http://hdl.handle.net/11449/189817 |
identifier_str_mv |
Biofouling, v. 34, n. 7, p. 815-825, 2018. 1029-2454 0892-7014 10.1080/08927014.2018.1504926 2-s2.0-85055084883 2640929291808415 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biofouling |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
815-825 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129159319257088 |