Nucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the Tilapiine cichlid fish, Oreochromis niloticus

Detalhes bibliográficos
Autor(a) principal: Martins, Cesar
Data de Publicação: 2000
Outros Autores: Wasko, Adriane P., Oliveira, Claudio [UNESP], Wright, Jonathan M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/j.1601-5223.2000.00039.x
http://hdl.handle.net/11449/66345
Resumo: In this study, we report the cloning and nucleotide sequence of PCR-generated 5S rDNA from the Tilapiine cichlid fish, Oreochromis niloticus. Two types of 5S rDNA were detected that differed by insertions and/or deletions and base substitutions within the non-transcribed spacer (NTS). Two 5S rDNA loci were observed by fluorescent in situ hybridization (FISH) in metaphase spreads of tilapia chromosomes. FISH using an 18S rDNA probe and silver nitrate sequential staining of 5S-FISH slides showed three 18S rDNA loci that are not syntenic to the 5S rDNA loci.
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spelling Nucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the Tilapiine cichlid fish, Oreochromis niloticusnucleotideribosome DNARNAspacer DNAanimal geneticsfemalegene deletiongene insertiongene locusgenetic analysismalemetaphase chromosomenonhumannucleotide sequenceTilapiaAnimalsBase SequenceBlotting, SouthernChromosome MappingChromosomesDNA PrimersDNA, RibosomalElectrophoresis, Agar GelIn Situ Hybridization, FluorescenceMetaphaseMolecular Sequence DataPerchesPolymerase Chain ReactionRNA, Ribosomal, 5SSequence Analysis, DNASequence Homology, Nucleic AcidAnimaliaCichlidaeOreochromis niloticusIn this study, we report the cloning and nucleotide sequence of PCR-generated 5S rDNA from the Tilapiine cichlid fish, Oreochromis niloticus. Two types of 5S rDNA were detected that differed by insertions and/or deletions and base substitutions within the non-transcribed spacer (NTS). Two 5S rDNA loci were observed by fluorescent in situ hybridization (FISH) in metaphase spreads of tilapia chromosomes. FISH using an 18S rDNA probe and silver nitrate sequential staining of 5S-FISH slides showed three 18S rDNA loci that are not syntenic to the 5S rDNA loci.Department of Biology Dalhousie University, Halifax, NS B3H 4J1Departamento de Genktica e Evoluqlo Universidade Federal de Slo Carlos, Slo Carlos, SPDepartamento de Morfologia Instituto de Biocihcias Universidade Estadual Paulista, Botucatu, SPDepartamento de Morfologia Instituto de Biocihcias Universidade Estadual Paulista, Botucatu, SPDalhousie UniversityUniversidade Federal de Slo CarlosUniversidade Estadual Paulista (Unesp)Martins, CesarWasko, Adriane P.Oliveira, Claudio [UNESP]Wright, Jonathan M.2014-05-27T11:19:59Z2014-05-27T11:19:59Z2000-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article39-46application/pdfhttp://dx.doi.org/10.1111/j.1601-5223.2000.00039.xHereditas, v. 133, n. 1, p. 39-46, 2000.0018-0661http://hdl.handle.net/11449/6634510.1111/j.1601-5223.2000.00039.x2-s2.0-00345105582-s2.0-0034510558.pdf885880069942535219268639173789090000-0003-4600-03670000-0003-3534-974XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHereditas0,278info:eu-repo/semantics/openAccess2023-11-06T06:06:35Zoai:repositorio.unesp.br:11449/66345Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-06T06:06:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Nucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the Tilapiine cichlid fish, Oreochromis niloticus
title Nucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the Tilapiine cichlid fish, Oreochromis niloticus
spellingShingle Nucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the Tilapiine cichlid fish, Oreochromis niloticus
Martins, Cesar
nucleotide
ribosome DNA
RNA
spacer DNA
animal genetics
female
gene deletion
gene insertion
gene locus
genetic analysis
male
metaphase chromosome
nonhuman
nucleotide sequence
Tilapia
Animals
Base Sequence
Blotting, Southern
Chromosome Mapping
Chromosomes
DNA Primers
DNA, Ribosomal
Electrophoresis, Agar Gel
In Situ Hybridization, Fluorescence
Metaphase
Molecular Sequence Data
Perches
Polymerase Chain Reaction
RNA, Ribosomal, 5S
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Animalia
Cichlidae
Oreochromis niloticus
title_short Nucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the Tilapiine cichlid fish, Oreochromis niloticus
title_full Nucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the Tilapiine cichlid fish, Oreochromis niloticus
title_fullStr Nucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the Tilapiine cichlid fish, Oreochromis niloticus
title_full_unstemmed Nucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the Tilapiine cichlid fish, Oreochromis niloticus
title_sort Nucleotide sequence of 5S rDNA and localization of the ribosomal RNA genes to metaphase chromosomes of the Tilapiine cichlid fish, Oreochromis niloticus
author Martins, Cesar
author_facet Martins, Cesar
Wasko, Adriane P.
Oliveira, Claudio [UNESP]
Wright, Jonathan M.
author_role author
author2 Wasko, Adriane P.
Oliveira, Claudio [UNESP]
Wright, Jonathan M.
author2_role author
author
author
dc.contributor.none.fl_str_mv Dalhousie University
Universidade Federal de Slo Carlos
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Martins, Cesar
Wasko, Adriane P.
Oliveira, Claudio [UNESP]
Wright, Jonathan M.
dc.subject.por.fl_str_mv nucleotide
ribosome DNA
RNA
spacer DNA
animal genetics
female
gene deletion
gene insertion
gene locus
genetic analysis
male
metaphase chromosome
nonhuman
nucleotide sequence
Tilapia
Animals
Base Sequence
Blotting, Southern
Chromosome Mapping
Chromosomes
DNA Primers
DNA, Ribosomal
Electrophoresis, Agar Gel
In Situ Hybridization, Fluorescence
Metaphase
Molecular Sequence Data
Perches
Polymerase Chain Reaction
RNA, Ribosomal, 5S
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Animalia
Cichlidae
Oreochromis niloticus
topic nucleotide
ribosome DNA
RNA
spacer DNA
animal genetics
female
gene deletion
gene insertion
gene locus
genetic analysis
male
metaphase chromosome
nonhuman
nucleotide sequence
Tilapia
Animals
Base Sequence
Blotting, Southern
Chromosome Mapping
Chromosomes
DNA Primers
DNA, Ribosomal
Electrophoresis, Agar Gel
In Situ Hybridization, Fluorescence
Metaphase
Molecular Sequence Data
Perches
Polymerase Chain Reaction
RNA, Ribosomal, 5S
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Animalia
Cichlidae
Oreochromis niloticus
description In this study, we report the cloning and nucleotide sequence of PCR-generated 5S rDNA from the Tilapiine cichlid fish, Oreochromis niloticus. Two types of 5S rDNA were detected that differed by insertions and/or deletions and base substitutions within the non-transcribed spacer (NTS). Two 5S rDNA loci were observed by fluorescent in situ hybridization (FISH) in metaphase spreads of tilapia chromosomes. FISH using an 18S rDNA probe and silver nitrate sequential staining of 5S-FISH slides showed three 18S rDNA loci that are not syntenic to the 5S rDNA loci.
publishDate 2000
dc.date.none.fl_str_mv 2000-12-01
2014-05-27T11:19:59Z
2014-05-27T11:19:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/j.1601-5223.2000.00039.x
Hereditas, v. 133, n. 1, p. 39-46, 2000.
0018-0661
http://hdl.handle.net/11449/66345
10.1111/j.1601-5223.2000.00039.x
2-s2.0-0034510558
2-s2.0-0034510558.pdf
8858800699425352
1926863917378909
0000-0003-4600-0367
0000-0003-3534-974X
url http://dx.doi.org/10.1111/j.1601-5223.2000.00039.x
http://hdl.handle.net/11449/66345
identifier_str_mv Hereditas, v. 133, n. 1, p. 39-46, 2000.
0018-0661
10.1111/j.1601-5223.2000.00039.x
2-s2.0-0034510558
2-s2.0-0034510558.pdf
8858800699425352
1926863917378909
0000-0003-4600-0367
0000-0003-3534-974X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Hereditas
0,278
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 39-46
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799964833510064128

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