Heterologous microsatellite primer pairs informative for the whole genus Arachis
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572006000400016 http://hdl.handle.net/11449/17948 |
Resumo: | The genus Arachis currently comprises 69 described species, some of which have potential and real value as human and animal foods. These Arachis species have been collected and maintained in germplasm banks to provide material that can be used as sources of genes in breeding programs and for the selection of new cultivars. One of the principal objectives of germplasm conservation is the evaluation of genetic variability, which is best conducted using molecular markers. We investigated the use of heterologous primers to amplify microsatellite loci that could be used to evaluate genetic variability in Arachis germplasm. Fifteen microsatellite primer pairs were tested in 76 accessions of 34 species from the nine Arachis sections. The data indicated that heterologous primers were very useful in Arachis since they had high transferability among the species (91%) and allowed the amplification of very polymorphic putative loci, which allowed both the characterization of most accessions and to make inferences about the mating systems of some species analyzed. Our data also revealed that the germplasm analyzed showed high variability, even when represented by few accessions. |
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Repositório Institucional da UNESP |
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Heterologous microsatellite primer pairs informative for the whole genus Arachispeanutgenetic relationshipmolecular markerswild relativesThe genus Arachis currently comprises 69 described species, some of which have potential and real value as human and animal foods. These Arachis species have been collected and maintained in germplasm banks to provide material that can be used as sources of genes in breeding programs and for the selection of new cultivars. One of the principal objectives of germplasm conservation is the evaluation of genetic variability, which is best conducted using molecular markers. We investigated the use of heterologous primers to amplify microsatellite loci that could be used to evaluate genetic variability in Arachis germplasm. Fifteen microsatellite primer pairs were tested in 76 accessions of 34 species from the nine Arachis sections. The data indicated that heterologous primers were very useful in Arachis since they had high transferability among the species (91%) and allowed the amplification of very polymorphic putative loci, which allowed both the characterization of most accessions and to make inferences about the mating systems of some species analyzed. Our data also revealed that the germplasm analyzed showed high variability, even when represented by few accessions.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Instituto de Biociências Departamento de GenéticaInstituto Agronômico de Campinas Centro de Pesquisa e Desenvolvimento de Recursos Genéticos e VegetaisUniversidade Estadual Paulista Instituto de Biociências Departamento de GenéticaSociedade Brasileira de GenéticaUniversidade Estadual Paulista (Unesp)Instituto Agronômico (IAC)Hoshino, Andrea Akemi [UNESP]Bravo, Juliana Pereira [UNESP]Angelici, Carla M.L.C.D. [UNESP]Barbosa, Andréa V. Gobbi [UNESP]Lopes, Catalina Romero [UNESP]Gimenes, Marcos A. [UNESP]2014-05-20T13:50:16Z2014-05-20T13:50:16Z2006-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article665-675application/pdfhttp://dx.doi.org/10.1590/S1415-47572006000400016Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 29, n. 4, p. 665-675, 2006.1415-4757http://hdl.handle.net/11449/1794810.1590/S1415-47572006000400016S1415-47572006000400016S1415-47572006000400016.pdf6903215575686010SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-10-18T06:05:54Zoai:repositorio.unesp.br:11449/17948Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:14:06.545998Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Heterologous microsatellite primer pairs informative for the whole genus Arachis |
title |
Heterologous microsatellite primer pairs informative for the whole genus Arachis |
spellingShingle |
Heterologous microsatellite primer pairs informative for the whole genus Arachis Hoshino, Andrea Akemi [UNESP] peanut genetic relationship molecular markers wild relatives |
title_short |
Heterologous microsatellite primer pairs informative for the whole genus Arachis |
title_full |
Heterologous microsatellite primer pairs informative for the whole genus Arachis |
title_fullStr |
Heterologous microsatellite primer pairs informative for the whole genus Arachis |
title_full_unstemmed |
Heterologous microsatellite primer pairs informative for the whole genus Arachis |
title_sort |
Heterologous microsatellite primer pairs informative for the whole genus Arachis |
author |
Hoshino, Andrea Akemi [UNESP] |
author_facet |
Hoshino, Andrea Akemi [UNESP] Bravo, Juliana Pereira [UNESP] Angelici, Carla M.L.C.D. [UNESP] Barbosa, Andréa V. Gobbi [UNESP] Lopes, Catalina Romero [UNESP] Gimenes, Marcos A. [UNESP] |
author_role |
author |
author2 |
Bravo, Juliana Pereira [UNESP] Angelici, Carla M.L.C.D. [UNESP] Barbosa, Andréa V. Gobbi [UNESP] Lopes, Catalina Romero [UNESP] Gimenes, Marcos A. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Instituto Agronômico (IAC) |
dc.contributor.author.fl_str_mv |
Hoshino, Andrea Akemi [UNESP] Bravo, Juliana Pereira [UNESP] Angelici, Carla M.L.C.D. [UNESP] Barbosa, Andréa V. Gobbi [UNESP] Lopes, Catalina Romero [UNESP] Gimenes, Marcos A. [UNESP] |
dc.subject.por.fl_str_mv |
peanut genetic relationship molecular markers wild relatives |
topic |
peanut genetic relationship molecular markers wild relatives |
description |
The genus Arachis currently comprises 69 described species, some of which have potential and real value as human and animal foods. These Arachis species have been collected and maintained in germplasm banks to provide material that can be used as sources of genes in breeding programs and for the selection of new cultivars. One of the principal objectives of germplasm conservation is the evaluation of genetic variability, which is best conducted using molecular markers. We investigated the use of heterologous primers to amplify microsatellite loci that could be used to evaluate genetic variability in Arachis germplasm. Fifteen microsatellite primer pairs were tested in 76 accessions of 34 species from the nine Arachis sections. The data indicated that heterologous primers were very useful in Arachis since they had high transferability among the species (91%) and allowed the amplification of very polymorphic putative loci, which allowed both the characterization of most accessions and to make inferences about the mating systems of some species analyzed. Our data also revealed that the germplasm analyzed showed high variability, even when represented by few accessions. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 2014-05-20T13:50:16Z 2014-05-20T13:50:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572006000400016 Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 29, n. 4, p. 665-675, 2006. 1415-4757 http://hdl.handle.net/11449/17948 10.1590/S1415-47572006000400016 S1415-47572006000400016 S1415-47572006000400016.pdf 6903215575686010 |
url |
http://dx.doi.org/10.1590/S1415-47572006000400016 http://hdl.handle.net/11449/17948 |
identifier_str_mv |
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 29, n. 4, p. 665-675, 2006. 1415-4757 10.1590/S1415-47572006000400016 S1415-47572006000400016 S1415-47572006000400016.pdf 6903215575686010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
665-675 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128484534386688 |