Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C

Detalhes bibliográficos
Autor(a) principal: Costa, Philipe [UNESP]
Data de Publicação: 2020
Outros Autores: Somensi, Lincon Bordignon, da Silva, Rita de Cássia Melo Vilhena de Andrade Fonseca, Mariano, Luísa Nathalia Bolda, Boeing, Thaise, Longo, Bruna [UNESP], Perfoll, Ellen, de Souza, Priscila, Gushiken, Lucas Fernando Sérgio [UNESP], Pellizzon, Cláudia Helena [UNESP], Rodrigues, Débora Munhoz, Bastos, Jairo Kenupp, de Andrade, Sérgio Faloni, da Silva, Luísa Mota
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10787-019-00649-7
http://hdl.handle.net/11449/199683
Resumo: Green propolis is a resinous substance used in folk medicine given its anti-inflammatory, antibacterial, and anti-ulcer effects. Our research group has already confirmed the gastroprotective activity of hydroalcoholic extract from green propolis (HEGP), as well as of its main isolated compounds. In continuity, this study evaluated the antioxidant mode of action involved in the preventive effect induced by HEGP, and its therapeutic gastric healing potential on installed ulcers. In addition, the healing effect of its main compound Artepillin C was also investigated. Acute and chronic ulcers were induced in rats by given ethanol or acetic acid, respectively. In acute model, the rats were orally pre-treated with vehicle (water plus 1% Tween, 1 mL/kg), HEGP (30–300 mg/kg), or carbenoxolone (200 mg/kg) 1 h prior the ulcer induction. In the chronic ulcer protocol, the rats received vehicle (water plus 1% Tween, 1 mL/kg), HEGP (300 mg/kg), or omeprazole (20 mg/kg) twice a day by 7 days, whereas groups of mice received vehicle (water plus 1% Tween, 1 mL/kg), Artepillin C (18 mg/kg), or ranitidine (20 mg/kg) twice a day by 4 days. Ulcerated tissue was collected for histological, histochemical, immunostaining, oxidative, and inflammatory analyses. The in vitro scavenger activity of HEGP was also verified using the DPPH assay. The oral pre-treatment with HEGP (100 and 300 mg/kg) prevented the gastric epithelial damage promoted by ethanol. Besides, HEGP (100 and 300 mg/kg) reduced SOD activity about 11% and 26%, respectively, and increased the activity of GST around 20% and CAT in 80%. HEGP (300 mg/kg) also reduced the production of reactive oxygen species, as well as lipoperoxidation levels in the ethanol-ulcerated tissue. In the acetic acid-induced chronic ulcer, the daily treatment with HEGP (300 mg/kg) accelerates the healing process by 71%. In this model, HEGP normalized SOD and CAT activity and increased GST activity by 109% when compared to non-ulcerated rats. In both models, the extract administration increased the mucin PAS staining and reduced the myeloperoxidase activity at the ulcer site. Moreover, the treatment with HEGP enhanced the PCNA immunostaining, but did not alter the concentration of collagen in the acetic acid-ulcerated tissue. The extract had a direct DPPH radical-scavenging ability (LogIC50: 0.56). Besides, as expected, HPLC analysis showed Artepillin C as a major compound and its administration at 18 mg/kg also accelerated the gastric healing ulcer process in mice. Our findings confirm that HEGP displays both gastroprotective and gastric healing properties, contributing to the validation of its popular use as preventive and therapeutic approaches. These actions occur through the increase in mucin production and the reestablishment of the oxidative balance due to a reduction in gastric inflammation.
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spelling Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin CGastric inflammationMucinOxidative stressPropolisGreen propolis is a resinous substance used in folk medicine given its anti-inflammatory, antibacterial, and anti-ulcer effects. Our research group has already confirmed the gastroprotective activity of hydroalcoholic extract from green propolis (HEGP), as well as of its main isolated compounds. In continuity, this study evaluated the antioxidant mode of action involved in the preventive effect induced by HEGP, and its therapeutic gastric healing potential on installed ulcers. In addition, the healing effect of its main compound Artepillin C was also investigated. Acute and chronic ulcers were induced in rats by given ethanol or acetic acid, respectively. In acute model, the rats were orally pre-treated with vehicle (water plus 1% Tween, 1 mL/kg), HEGP (30–300 mg/kg), or carbenoxolone (200 mg/kg) 1 h prior the ulcer induction. In the chronic ulcer protocol, the rats received vehicle (water plus 1% Tween, 1 mL/kg), HEGP (300 mg/kg), or omeprazole (20 mg/kg) twice a day by 7 days, whereas groups of mice received vehicle (water plus 1% Tween, 1 mL/kg), Artepillin C (18 mg/kg), or ranitidine (20 mg/kg) twice a day by 4 days. Ulcerated tissue was collected for histological, histochemical, immunostaining, oxidative, and inflammatory analyses. The in vitro scavenger activity of HEGP was also verified using the DPPH assay. The oral pre-treatment with HEGP (100 and 300 mg/kg) prevented the gastric epithelial damage promoted by ethanol. Besides, HEGP (100 and 300 mg/kg) reduced SOD activity about 11% and 26%, respectively, and increased the activity of GST around 20% and CAT in 80%. HEGP (300 mg/kg) also reduced the production of reactive oxygen species, as well as lipoperoxidation levels in the ethanol-ulcerated tissue. In the acetic acid-induced chronic ulcer, the daily treatment with HEGP (300 mg/kg) accelerates the healing process by 71%. In this model, HEGP normalized SOD and CAT activity and increased GST activity by 109% when compared to non-ulcerated rats. In both models, the extract administration increased the mucin PAS staining and reduced the myeloperoxidase activity at the ulcer site. Moreover, the treatment with HEGP enhanced the PCNA immunostaining, but did not alter the concentration of collagen in the acetic acid-ulcerated tissue. The extract had a direct DPPH radical-scavenging ability (LogIC50: 0.56). Besides, as expected, HPLC analysis showed Artepillin C as a major compound and its administration at 18 mg/kg also accelerated the gastric healing ulcer process in mice. Our findings confirm that HEGP displays both gastroprotective and gastric healing properties, contributing to the validation of its popular use as preventive and therapeutic approaches. These actions occur through the increase in mucin production and the reestablishment of the oxidative balance due to a reduction in gastric inflammation.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Programa de Pós-Graduação em Ciências Farmacêuticas Núcleo de Investigações Químico-Farmacêuticas (NIQFAR) Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458Departamento de Morfologia Universidade do Estado de São Paulo (Unesp) Instituto de Biociências Botucatu, Rua Professor Antônio Celso Wagner Zanin s/nFaculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo (USP), Avenida do Café, s/nDepartamento de Morfologia Universidade do Estado de São Paulo (Unesp) Instituto de Biociências Botucatu, Rua Professor Antônio Celso Wagner Zanin s/nCAPES: 001FAPESP: 2017/04138-8Universidade do Vale do Itajaí (UNIVALI)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Costa, Philipe [UNESP]Somensi, Lincon Bordignonda Silva, Rita de Cássia Melo Vilhena de Andrade FonsecaMariano, Luísa Nathalia BoldaBoeing, ThaiseLongo, Bruna [UNESP]Perfoll, Ellende Souza, PriscilaGushiken, Lucas Fernando Sérgio [UNESP]Pellizzon, Cláudia Helena [UNESP]Rodrigues, Débora MunhozBastos, Jairo Kenuppde Andrade, Sérgio Falonida Silva, Luísa Mota2020-12-12T01:46:27Z2020-12-12T01:46:27Z2020-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1009-1025http://dx.doi.org/10.1007/s10787-019-00649-7Inflammopharmacology, v. 28, n. 4, p. 1009-1025, 2020.1568-56080925-4692http://hdl.handle.net/11449/19968310.1007/s10787-019-00649-72-s2.0-8507525958300193937798010690000-0002-4494-4180Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInflammopharmacologyinfo:eu-repo/semantics/openAccess2021-10-28T16:42:18Zoai:repositorio.unesp.br:11449/199683Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-28T16:42:18Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C
title Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C
spellingShingle Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C
Costa, Philipe [UNESP]
Gastric inflammation
Mucin
Oxidative stress
Propolis
title_short Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C
title_full Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C
title_fullStr Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C
title_full_unstemmed Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C
title_sort Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C
author Costa, Philipe [UNESP]
author_facet Costa, Philipe [UNESP]
Somensi, Lincon Bordignon
da Silva, Rita de Cássia Melo Vilhena de Andrade Fonseca
Mariano, Luísa Nathalia Bolda
Boeing, Thaise
Longo, Bruna [UNESP]
Perfoll, Ellen
de Souza, Priscila
Gushiken, Lucas Fernando Sérgio [UNESP]
Pellizzon, Cláudia Helena [UNESP]
Rodrigues, Débora Munhoz
Bastos, Jairo Kenupp
de Andrade, Sérgio Faloni
da Silva, Luísa Mota
author_role author
author2 Somensi, Lincon Bordignon
da Silva, Rita de Cássia Melo Vilhena de Andrade Fonseca
Mariano, Luísa Nathalia Bolda
Boeing, Thaise
Longo, Bruna [UNESP]
Perfoll, Ellen
de Souza, Priscila
Gushiken, Lucas Fernando Sérgio [UNESP]
Pellizzon, Cláudia Helena [UNESP]
Rodrigues, Débora Munhoz
Bastos, Jairo Kenupp
de Andrade, Sérgio Faloni
da Silva, Luísa Mota
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Vale do Itajaí (UNIVALI)
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Costa, Philipe [UNESP]
Somensi, Lincon Bordignon
da Silva, Rita de Cássia Melo Vilhena de Andrade Fonseca
Mariano, Luísa Nathalia Bolda
Boeing, Thaise
Longo, Bruna [UNESP]
Perfoll, Ellen
de Souza, Priscila
Gushiken, Lucas Fernando Sérgio [UNESP]
Pellizzon, Cláudia Helena [UNESP]
Rodrigues, Débora Munhoz
Bastos, Jairo Kenupp
de Andrade, Sérgio Faloni
da Silva, Luísa Mota
dc.subject.por.fl_str_mv Gastric inflammation
Mucin
Oxidative stress
Propolis
topic Gastric inflammation
Mucin
Oxidative stress
Propolis
description Green propolis is a resinous substance used in folk medicine given its anti-inflammatory, antibacterial, and anti-ulcer effects. Our research group has already confirmed the gastroprotective activity of hydroalcoholic extract from green propolis (HEGP), as well as of its main isolated compounds. In continuity, this study evaluated the antioxidant mode of action involved in the preventive effect induced by HEGP, and its therapeutic gastric healing potential on installed ulcers. In addition, the healing effect of its main compound Artepillin C was also investigated. Acute and chronic ulcers were induced in rats by given ethanol or acetic acid, respectively. In acute model, the rats were orally pre-treated with vehicle (water plus 1% Tween, 1 mL/kg), HEGP (30–300 mg/kg), or carbenoxolone (200 mg/kg) 1 h prior the ulcer induction. In the chronic ulcer protocol, the rats received vehicle (water plus 1% Tween, 1 mL/kg), HEGP (300 mg/kg), or omeprazole (20 mg/kg) twice a day by 7 days, whereas groups of mice received vehicle (water plus 1% Tween, 1 mL/kg), Artepillin C (18 mg/kg), or ranitidine (20 mg/kg) twice a day by 4 days. Ulcerated tissue was collected for histological, histochemical, immunostaining, oxidative, and inflammatory analyses. The in vitro scavenger activity of HEGP was also verified using the DPPH assay. The oral pre-treatment with HEGP (100 and 300 mg/kg) prevented the gastric epithelial damage promoted by ethanol. Besides, HEGP (100 and 300 mg/kg) reduced SOD activity about 11% and 26%, respectively, and increased the activity of GST around 20% and CAT in 80%. HEGP (300 mg/kg) also reduced the production of reactive oxygen species, as well as lipoperoxidation levels in the ethanol-ulcerated tissue. In the acetic acid-induced chronic ulcer, the daily treatment with HEGP (300 mg/kg) accelerates the healing process by 71%. In this model, HEGP normalized SOD and CAT activity and increased GST activity by 109% when compared to non-ulcerated rats. In both models, the extract administration increased the mucin PAS staining and reduced the myeloperoxidase activity at the ulcer site. Moreover, the treatment with HEGP enhanced the PCNA immunostaining, but did not alter the concentration of collagen in the acetic acid-ulcerated tissue. The extract had a direct DPPH radical-scavenging ability (LogIC50: 0.56). Besides, as expected, HPLC analysis showed Artepillin C as a major compound and its administration at 18 mg/kg also accelerated the gastric healing ulcer process in mice. Our findings confirm that HEGP displays both gastroprotective and gastric healing properties, contributing to the validation of its popular use as preventive and therapeutic approaches. These actions occur through the increase in mucin production and the reestablishment of the oxidative balance due to a reduction in gastric inflammation.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:46:27Z
2020-12-12T01:46:27Z
2020-08-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10787-019-00649-7
Inflammopharmacology, v. 28, n. 4, p. 1009-1025, 2020.
1568-5608
0925-4692
http://hdl.handle.net/11449/199683
10.1007/s10787-019-00649-7
2-s2.0-85075259583
0019393779801069
0000-0002-4494-4180
url http://dx.doi.org/10.1007/s10787-019-00649-7
http://hdl.handle.net/11449/199683
identifier_str_mv Inflammopharmacology, v. 28, n. 4, p. 1009-1025, 2020.
1568-5608
0925-4692
10.1007/s10787-019-00649-7
2-s2.0-85075259583
0019393779801069
0000-0002-4494-4180
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Inflammopharmacology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1009-1025
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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