Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10787-019-00649-7 http://hdl.handle.net/11449/199683 |
Resumo: | Green propolis is a resinous substance used in folk medicine given its anti-inflammatory, antibacterial, and anti-ulcer effects. Our research group has already confirmed the gastroprotective activity of hydroalcoholic extract from green propolis (HEGP), as well as of its main isolated compounds. In continuity, this study evaluated the antioxidant mode of action involved in the preventive effect induced by HEGP, and its therapeutic gastric healing potential on installed ulcers. In addition, the healing effect of its main compound Artepillin C was also investigated. Acute and chronic ulcers were induced in rats by given ethanol or acetic acid, respectively. In acute model, the rats were orally pre-treated with vehicle (water plus 1% Tween, 1 mL/kg), HEGP (30–300 mg/kg), or carbenoxolone (200 mg/kg) 1 h prior the ulcer induction. In the chronic ulcer protocol, the rats received vehicle (water plus 1% Tween, 1 mL/kg), HEGP (300 mg/kg), or omeprazole (20 mg/kg) twice a day by 7 days, whereas groups of mice received vehicle (water plus 1% Tween, 1 mL/kg), Artepillin C (18 mg/kg), or ranitidine (20 mg/kg) twice a day by 4 days. Ulcerated tissue was collected for histological, histochemical, immunostaining, oxidative, and inflammatory analyses. The in vitro scavenger activity of HEGP was also verified using the DPPH assay. The oral pre-treatment with HEGP (100 and 300 mg/kg) prevented the gastric epithelial damage promoted by ethanol. Besides, HEGP (100 and 300 mg/kg) reduced SOD activity about 11% and 26%, respectively, and increased the activity of GST around 20% and CAT in 80%. HEGP (300 mg/kg) also reduced the production of reactive oxygen species, as well as lipoperoxidation levels in the ethanol-ulcerated tissue. In the acetic acid-induced chronic ulcer, the daily treatment with HEGP (300 mg/kg) accelerates the healing process by 71%. In this model, HEGP normalized SOD and CAT activity and increased GST activity by 109% when compared to non-ulcerated rats. In both models, the extract administration increased the mucin PAS staining and reduced the myeloperoxidase activity at the ulcer site. Moreover, the treatment with HEGP enhanced the PCNA immunostaining, but did not alter the concentration of collagen in the acetic acid-ulcerated tissue. The extract had a direct DPPH radical-scavenging ability (LogIC50: 0.56). Besides, as expected, HPLC analysis showed Artepillin C as a major compound and its administration at 18 mg/kg also accelerated the gastric healing ulcer process in mice. Our findings confirm that HEGP displays both gastroprotective and gastric healing properties, contributing to the validation of its popular use as preventive and therapeutic approaches. These actions occur through the increase in mucin production and the reestablishment of the oxidative balance due to a reduction in gastric inflammation. |
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Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin CGastric inflammationMucinOxidative stressPropolisGreen propolis is a resinous substance used in folk medicine given its anti-inflammatory, antibacterial, and anti-ulcer effects. Our research group has already confirmed the gastroprotective activity of hydroalcoholic extract from green propolis (HEGP), as well as of its main isolated compounds. In continuity, this study evaluated the antioxidant mode of action involved in the preventive effect induced by HEGP, and its therapeutic gastric healing potential on installed ulcers. In addition, the healing effect of its main compound Artepillin C was also investigated. Acute and chronic ulcers were induced in rats by given ethanol or acetic acid, respectively. In acute model, the rats were orally pre-treated with vehicle (water plus 1% Tween, 1 mL/kg), HEGP (30–300 mg/kg), or carbenoxolone (200 mg/kg) 1 h prior the ulcer induction. In the chronic ulcer protocol, the rats received vehicle (water plus 1% Tween, 1 mL/kg), HEGP (300 mg/kg), or omeprazole (20 mg/kg) twice a day by 7 days, whereas groups of mice received vehicle (water plus 1% Tween, 1 mL/kg), Artepillin C (18 mg/kg), or ranitidine (20 mg/kg) twice a day by 4 days. Ulcerated tissue was collected for histological, histochemical, immunostaining, oxidative, and inflammatory analyses. The in vitro scavenger activity of HEGP was also verified using the DPPH assay. The oral pre-treatment with HEGP (100 and 300 mg/kg) prevented the gastric epithelial damage promoted by ethanol. Besides, HEGP (100 and 300 mg/kg) reduced SOD activity about 11% and 26%, respectively, and increased the activity of GST around 20% and CAT in 80%. HEGP (300 mg/kg) also reduced the production of reactive oxygen species, as well as lipoperoxidation levels in the ethanol-ulcerated tissue. In the acetic acid-induced chronic ulcer, the daily treatment with HEGP (300 mg/kg) accelerates the healing process by 71%. In this model, HEGP normalized SOD and CAT activity and increased GST activity by 109% when compared to non-ulcerated rats. In both models, the extract administration increased the mucin PAS staining and reduced the myeloperoxidase activity at the ulcer site. Moreover, the treatment with HEGP enhanced the PCNA immunostaining, but did not alter the concentration of collagen in the acetic acid-ulcerated tissue. The extract had a direct DPPH radical-scavenging ability (LogIC50: 0.56). Besides, as expected, HPLC analysis showed Artepillin C as a major compound and its administration at 18 mg/kg also accelerated the gastric healing ulcer process in mice. Our findings confirm that HEGP displays both gastroprotective and gastric healing properties, contributing to the validation of its popular use as preventive and therapeutic approaches. These actions occur through the increase in mucin production and the reestablishment of the oxidative balance due to a reduction in gastric inflammation.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Programa de Pós-Graduação em Ciências Farmacêuticas Núcleo de Investigações Químico-Farmacêuticas (NIQFAR) Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458Departamento de Morfologia Universidade do Estado de São Paulo (Unesp) Instituto de Biociências Botucatu, Rua Professor Antônio Celso Wagner Zanin s/nFaculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo (USP), Avenida do Café, s/nDepartamento de Morfologia Universidade do Estado de São Paulo (Unesp) Instituto de Biociências Botucatu, Rua Professor Antônio Celso Wagner Zanin s/nCAPES: 001FAPESP: 2017/04138-8Universidade do Vale do Itajaí (UNIVALI)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Costa, Philipe [UNESP]Somensi, Lincon Bordignonda Silva, Rita de Cássia Melo Vilhena de Andrade FonsecaMariano, Luísa Nathalia BoldaBoeing, ThaiseLongo, Bruna [UNESP]Perfoll, Ellende Souza, PriscilaGushiken, Lucas Fernando Sérgio [UNESP]Pellizzon, Cláudia Helena [UNESP]Rodrigues, Débora MunhozBastos, Jairo Kenuppde Andrade, Sérgio Falonida Silva, Luísa Mota2020-12-12T01:46:27Z2020-12-12T01:46:27Z2020-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1009-1025http://dx.doi.org/10.1007/s10787-019-00649-7Inflammopharmacology, v. 28, n. 4, p. 1009-1025, 2020.1568-56080925-4692http://hdl.handle.net/11449/19968310.1007/s10787-019-00649-72-s2.0-8507525958300193937798010690000-0002-4494-4180Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInflammopharmacologyinfo:eu-repo/semantics/openAccess2021-10-28T16:42:18Zoai:repositorio.unesp.br:11449/199683Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:13:40.892872Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C |
title |
Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C |
spellingShingle |
Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C Costa, Philipe [UNESP] Gastric inflammation Mucin Oxidative stress Propolis |
title_short |
Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C |
title_full |
Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C |
title_fullStr |
Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C |
title_full_unstemmed |
Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C |
title_sort |
Role of the antioxidant properties in the gastroprotective and gastric healing activity promoted by Brazilian green propolis and the healing efficacy of Artepillin C |
author |
Costa, Philipe [UNESP] |
author_facet |
Costa, Philipe [UNESP] Somensi, Lincon Bordignon da Silva, Rita de Cássia Melo Vilhena de Andrade Fonseca Mariano, Luísa Nathalia Bolda Boeing, Thaise Longo, Bruna [UNESP] Perfoll, Ellen de Souza, Priscila Gushiken, Lucas Fernando Sérgio [UNESP] Pellizzon, Cláudia Helena [UNESP] Rodrigues, Débora Munhoz Bastos, Jairo Kenupp de Andrade, Sérgio Faloni da Silva, Luísa Mota |
author_role |
author |
author2 |
Somensi, Lincon Bordignon da Silva, Rita de Cássia Melo Vilhena de Andrade Fonseca Mariano, Luísa Nathalia Bolda Boeing, Thaise Longo, Bruna [UNESP] Perfoll, Ellen de Souza, Priscila Gushiken, Lucas Fernando Sérgio [UNESP] Pellizzon, Cláudia Helena [UNESP] Rodrigues, Débora Munhoz Bastos, Jairo Kenupp de Andrade, Sérgio Faloni da Silva, Luísa Mota |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Vale do Itajaí (UNIVALI) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Costa, Philipe [UNESP] Somensi, Lincon Bordignon da Silva, Rita de Cássia Melo Vilhena de Andrade Fonseca Mariano, Luísa Nathalia Bolda Boeing, Thaise Longo, Bruna [UNESP] Perfoll, Ellen de Souza, Priscila Gushiken, Lucas Fernando Sérgio [UNESP] Pellizzon, Cláudia Helena [UNESP] Rodrigues, Débora Munhoz Bastos, Jairo Kenupp de Andrade, Sérgio Faloni da Silva, Luísa Mota |
dc.subject.por.fl_str_mv |
Gastric inflammation Mucin Oxidative stress Propolis |
topic |
Gastric inflammation Mucin Oxidative stress Propolis |
description |
Green propolis is a resinous substance used in folk medicine given its anti-inflammatory, antibacterial, and anti-ulcer effects. Our research group has already confirmed the gastroprotective activity of hydroalcoholic extract from green propolis (HEGP), as well as of its main isolated compounds. In continuity, this study evaluated the antioxidant mode of action involved in the preventive effect induced by HEGP, and its therapeutic gastric healing potential on installed ulcers. In addition, the healing effect of its main compound Artepillin C was also investigated. Acute and chronic ulcers were induced in rats by given ethanol or acetic acid, respectively. In acute model, the rats were orally pre-treated with vehicle (water plus 1% Tween, 1 mL/kg), HEGP (30–300 mg/kg), or carbenoxolone (200 mg/kg) 1 h prior the ulcer induction. In the chronic ulcer protocol, the rats received vehicle (water plus 1% Tween, 1 mL/kg), HEGP (300 mg/kg), or omeprazole (20 mg/kg) twice a day by 7 days, whereas groups of mice received vehicle (water plus 1% Tween, 1 mL/kg), Artepillin C (18 mg/kg), or ranitidine (20 mg/kg) twice a day by 4 days. Ulcerated tissue was collected for histological, histochemical, immunostaining, oxidative, and inflammatory analyses. The in vitro scavenger activity of HEGP was also verified using the DPPH assay. The oral pre-treatment with HEGP (100 and 300 mg/kg) prevented the gastric epithelial damage promoted by ethanol. Besides, HEGP (100 and 300 mg/kg) reduced SOD activity about 11% and 26%, respectively, and increased the activity of GST around 20% and CAT in 80%. HEGP (300 mg/kg) also reduced the production of reactive oxygen species, as well as lipoperoxidation levels in the ethanol-ulcerated tissue. In the acetic acid-induced chronic ulcer, the daily treatment with HEGP (300 mg/kg) accelerates the healing process by 71%. In this model, HEGP normalized SOD and CAT activity and increased GST activity by 109% when compared to non-ulcerated rats. In both models, the extract administration increased the mucin PAS staining and reduced the myeloperoxidase activity at the ulcer site. Moreover, the treatment with HEGP enhanced the PCNA immunostaining, but did not alter the concentration of collagen in the acetic acid-ulcerated tissue. The extract had a direct DPPH radical-scavenging ability (LogIC50: 0.56). Besides, as expected, HPLC analysis showed Artepillin C as a major compound and its administration at 18 mg/kg also accelerated the gastric healing ulcer process in mice. Our findings confirm that HEGP displays both gastroprotective and gastric healing properties, contributing to the validation of its popular use as preventive and therapeutic approaches. These actions occur through the increase in mucin production and the reestablishment of the oxidative balance due to a reduction in gastric inflammation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:46:27Z 2020-12-12T01:46:27Z 2020-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10787-019-00649-7 Inflammopharmacology, v. 28, n. 4, p. 1009-1025, 2020. 1568-5608 0925-4692 http://hdl.handle.net/11449/199683 10.1007/s10787-019-00649-7 2-s2.0-85075259583 0019393779801069 0000-0002-4494-4180 |
url |
http://dx.doi.org/10.1007/s10787-019-00649-7 http://hdl.handle.net/11449/199683 |
identifier_str_mv |
Inflammopharmacology, v. 28, n. 4, p. 1009-1025, 2020. 1568-5608 0925-4692 10.1007/s10787-019-00649-7 2-s2.0-85075259583 0019393779801069 0000-0002-4494-4180 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Inflammopharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1009-1025 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128621787742208 |