Study of visceral antinociceptive potential of bee Apis mellifera venom
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5897/ajpp2013.3989 http://hdl.handle.net/11449/137316 |
Resumo: | Pain is one of the most common reasons for patients to seek medical care. Bee Apis mellifera venom (AMV) has traditionally been used to treat inflammatory diseases and the alleviation of pain. Herein, we aimed to investigate the visceral antinociceptive potential of A. mellifera bee venom and its possible mechanism of action. Acetic acid-induced writhing assay was used in mice to determine the degree of visceral antinociception. Visceral antinociceptive activity was expressed as the reduction in the number of abdominal constrictions. Mice received an intraperitoneal injection of acetic acid after administration of AMV (0.08 or 0.8 mg/kg; intraperitoneally (i.p.)). In mechanistic studies, separate experiments were realized to examine the role of α2-receptors, nitric oxide, calcium channels, K+ATP channel activation, TRPV1 and opioid receptors on the visceral antinociceptive effect of AMV (0.8 mg/kg), using appropriate antagonists, yohimbine (2 mg/kg), L-NG-Nitroarginine methyl ester (L-NAME, 10 mg/kg), verapamil (5 mg/kg), glibenclamide (5 mg/kg), ruthenium red (3 mg/kg) or naloxone (2 mg/kg). AMV presented visceral antinociceptive activity in both doses tested (0.08 and 0.8 mg/Kg). Visceral antinociceptive effect of AMV was resistant to all the antagonists used. Mice showed no significant alterations in locomotion frequency, indicating that the observed antinociception is not a consequence of motor abnormality. Although AMV efficient diminished the acetic acid-evoked pain-related behavior, its mechanism is unclear from this study and future studies are needed to verify how the venom exerts its antinociceptive action. |
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Study of visceral antinociceptive potential of bee Apis mellifera venomAppis mellifera venomAntinociceptiveVisceral painPain is one of the most common reasons for patients to seek medical care. Bee Apis mellifera venom (AMV) has traditionally been used to treat inflammatory diseases and the alleviation of pain. Herein, we aimed to investigate the visceral antinociceptive potential of A. mellifera bee venom and its possible mechanism of action. Acetic acid-induced writhing assay was used in mice to determine the degree of visceral antinociception. Visceral antinociceptive activity was expressed as the reduction in the number of abdominal constrictions. Mice received an intraperitoneal injection of acetic acid after administration of AMV (0.08 or 0.8 mg/kg; intraperitoneally (i.p.)). In mechanistic studies, separate experiments were realized to examine the role of α2-receptors, nitric oxide, calcium channels, K+ATP channel activation, TRPV1 and opioid receptors on the visceral antinociceptive effect of AMV (0.8 mg/kg), using appropriate antagonists, yohimbine (2 mg/kg), L-NG-Nitroarginine methyl ester (L-NAME, 10 mg/kg), verapamil (5 mg/kg), glibenclamide (5 mg/kg), ruthenium red (3 mg/kg) or naloxone (2 mg/kg). AMV presented visceral antinociceptive activity in both doses tested (0.08 and 0.8 mg/Kg). Visceral antinociceptive effect of AMV was resistant to all the antagonists used. Mice showed no significant alterations in locomotion frequency, indicating that the observed antinociception is not a consequence of motor abnormality. Although AMV efficient diminished the acetic acid-evoked pain-related behavior, its mechanism is unclear from this study and future studies are needed to verify how the venom exerts its antinociceptive action.Universidade Federal do Ceará, Fortaleza, Ceará, BrasilUniversidade de Fortaleza, Fortaleza, Ceará, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Ciências Biológicas, Instituto de Biociências - São Vicente, Sao Vicente, Praça Infante Dom Henrique s/nº - Parque Bitaru, Parque Bitaru, CEP 11330-900, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Ciências Biológicas, Instituto de Biociências - São Vicente, Sao Vicente, Praça Infante Dom Henrique s/nº - Parque Bitaru, Parque Bitaru, CEP 11330-900, SP, BrasilUniversidade Federal do Ceará (UFC)Universidade de Fortaleza (UNIFOR)Universidade Estadual Paulista (Unesp)Costa, Marcus F. B.Campos, Adriana R.Abdon, Ana P. V.Vasconcelos, Renata P.Castro, Carolina A.Toyama, Marcos Hikari [UNESP]Monteiro, Helena SaMartins, Alice M. C.2016-04-01T18:45:10Z2016-04-01T18:45:10Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article781-785application/pdfhttp://dx.doi.org/10.5897/ajpp2013.3989African Journal of Pharmacy and Pharmacology, v. 8, n. 30, p. 781-785, 2014.1996-0816http://hdl.handle.net/11449/13731610.5897/ajpp2013.3989ISSN1996-0816-2014-08-30-781-785.pdf8573195327542061Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAfrican Journal of Pharmacy and Pharmacologyinfo:eu-repo/semantics/openAccess2023-11-13T06:11:13Zoai:repositorio.unesp.br:11449/137316Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-13T06:11:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Study of visceral antinociceptive potential of bee Apis mellifera venom |
title |
Study of visceral antinociceptive potential of bee Apis mellifera venom |
spellingShingle |
Study of visceral antinociceptive potential of bee Apis mellifera venom Costa, Marcus F. B. Appis mellifera venom Antinociceptive Visceral pain |
title_short |
Study of visceral antinociceptive potential of bee Apis mellifera venom |
title_full |
Study of visceral antinociceptive potential of bee Apis mellifera venom |
title_fullStr |
Study of visceral antinociceptive potential of bee Apis mellifera venom |
title_full_unstemmed |
Study of visceral antinociceptive potential of bee Apis mellifera venom |
title_sort |
Study of visceral antinociceptive potential of bee Apis mellifera venom |
author |
Costa, Marcus F. B. |
author_facet |
Costa, Marcus F. B. Campos, Adriana R. Abdon, Ana P. V. Vasconcelos, Renata P. Castro, Carolina A. Toyama, Marcos Hikari [UNESP] Monteiro, Helena Sa Martins, Alice M. C. |
author_role |
author |
author2 |
Campos, Adriana R. Abdon, Ana P. V. Vasconcelos, Renata P. Castro, Carolina A. Toyama, Marcos Hikari [UNESP] Monteiro, Helena Sa Martins, Alice M. C. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Ceará (UFC) Universidade de Fortaleza (UNIFOR) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Costa, Marcus F. B. Campos, Adriana R. Abdon, Ana P. V. Vasconcelos, Renata P. Castro, Carolina A. Toyama, Marcos Hikari [UNESP] Monteiro, Helena Sa Martins, Alice M. C. |
dc.subject.por.fl_str_mv |
Appis mellifera venom Antinociceptive Visceral pain |
topic |
Appis mellifera venom Antinociceptive Visceral pain |
description |
Pain is one of the most common reasons for patients to seek medical care. Bee Apis mellifera venom (AMV) has traditionally been used to treat inflammatory diseases and the alleviation of pain. Herein, we aimed to investigate the visceral antinociceptive potential of A. mellifera bee venom and its possible mechanism of action. Acetic acid-induced writhing assay was used in mice to determine the degree of visceral antinociception. Visceral antinociceptive activity was expressed as the reduction in the number of abdominal constrictions. Mice received an intraperitoneal injection of acetic acid after administration of AMV (0.08 or 0.8 mg/kg; intraperitoneally (i.p.)). In mechanistic studies, separate experiments were realized to examine the role of α2-receptors, nitric oxide, calcium channels, K+ATP channel activation, TRPV1 and opioid receptors on the visceral antinociceptive effect of AMV (0.8 mg/kg), using appropriate antagonists, yohimbine (2 mg/kg), L-NG-Nitroarginine methyl ester (L-NAME, 10 mg/kg), verapamil (5 mg/kg), glibenclamide (5 mg/kg), ruthenium red (3 mg/kg) or naloxone (2 mg/kg). AMV presented visceral antinociceptive activity in both doses tested (0.08 and 0.8 mg/Kg). Visceral antinociceptive effect of AMV was resistant to all the antagonists used. Mice showed no significant alterations in locomotion frequency, indicating that the observed antinociception is not a consequence of motor abnormality. Although AMV efficient diminished the acetic acid-evoked pain-related behavior, its mechanism is unclear from this study and future studies are needed to verify how the venom exerts its antinociceptive action. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2016-04-01T18:45:10Z 2016-04-01T18:45:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5897/ajpp2013.3989 African Journal of Pharmacy and Pharmacology, v. 8, n. 30, p. 781-785, 2014. 1996-0816 http://hdl.handle.net/11449/137316 10.5897/ajpp2013.3989 ISSN1996-0816-2014-08-30-781-785.pdf 8573195327542061 |
url |
http://dx.doi.org/10.5897/ajpp2013.3989 http://hdl.handle.net/11449/137316 |
identifier_str_mv |
African Journal of Pharmacy and Pharmacology, v. 8, n. 30, p. 781-785, 2014. 1996-0816 10.5897/ajpp2013.3989 ISSN1996-0816-2014-08-30-781-785.pdf 8573195327542061 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
African Journal of Pharmacy and Pharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
781-785 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964919671554048 |