Anti-Helicobacter pylori activity and immunostimulatory effect of extracts from Byrsonima crassa Nied. (Malpighiaceae)
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1472-6882-9-2 http://hdl.handle.net/11449/7372 |
Resumo: | Background: Several in vitro studies have looked at the effect of medicinal plant extracts against Helicobacter pylori (H. pylori). Regardless of the popular use of Byrsonima crassa (B. crassa) as antiemetic, diuretic, febrifuge, to treat diarrhea, gastritis and ulcers, there is no data on its effects against H. pylori. In this study, we evaluated the anti-H. pylori of B. crassa leaves extracts and its effects on reactive oxygen/nitrogen intermediates induction by murine peritoneal macrophages.Methods: The minimal inhibitory concentration (MIC) was determined by broth microdilution method and the production of hydrogen peroxide (H(2)O(2)) and nitric oxide (NO) by the horseradish peroxidase-dependent oxidation of phenol red and Griess reaction, respectively.Results: The methanolic (MeOH) and chloroformic (CHCl(3)) extracts inhibit, in vitro, the growth of H. pylori with MIC value of 1024 mu g/ml. The MeOH extract induced the production H(2)O(2) and NO, but CHCl(3) extract only NO.Conclusion: Based in our results, B. crassa can be considered a source of compounds with anti-H. pylori activity, but its use should be done with caution in treatment of the gastritis and peptic ulcers, since the reactive oxygen/nitrogen intermediates are involved in the pathogenesis of gastric mucosal injury induced by ulcerogenic agents and H. pylori infections. |
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Anti-Helicobacter pylori activity and immunostimulatory effect of extracts from Byrsonima crassa Nied. (Malpighiaceae)Background: Several in vitro studies have looked at the effect of medicinal plant extracts against Helicobacter pylori (H. pylori). Regardless of the popular use of Byrsonima crassa (B. crassa) as antiemetic, diuretic, febrifuge, to treat diarrhea, gastritis and ulcers, there is no data on its effects against H. pylori. In this study, we evaluated the anti-H. pylori of B. crassa leaves extracts and its effects on reactive oxygen/nitrogen intermediates induction by murine peritoneal macrophages.Methods: The minimal inhibitory concentration (MIC) was determined by broth microdilution method and the production of hydrogen peroxide (H(2)O(2)) and nitric oxide (NO) by the horseradish peroxidase-dependent oxidation of phenol red and Griess reaction, respectively.Results: The methanolic (MeOH) and chloroformic (CHCl(3)) extracts inhibit, in vitro, the growth of H. pylori with MIC value of 1024 mu g/ml. The MeOH extract induced the production H(2)O(2) and NO, but CHCl(3) extract only NO.Conclusion: Based in our results, B. crassa can be considered a source of compounds with anti-H. pylori activity, but its use should be done with caution in treatment of the gastritis and peptic ulcers, since the reactive oxygen/nitrogen intermediates are involved in the pathogenesis of gastric mucosal injury induced by ulcerogenic agents and H. pylori infections.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Anal Clin, BR-14801960 Araraquara, SP, BrazilUniv Estadual Paulista, Inst Quim, Dept Quim Organ, BR-14800900 Araraquara, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut, Dept Anal Clin, BR-14801960 Araraquara, SP, BrazilUniv Estadual Paulista, Inst Quim, Dept Quim Organ, BR-14800900 Araraquara, SP, BrazilBiomed Central Ltd.Universidade Estadual Paulista (Unesp)Bonacorsi, Cibele [UNESP]Raddi, Maria Stella G. [UNESP]Carlos, Iracilda Z. [UNESP]Sannomiya, Miriam [UNESP]Vilegas, Wagner [UNESP]2014-05-20T13:24:02Z2014-05-20T13:24:02Z2009-01-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://dx.doi.org/10.1186/1472-6882-9-2Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 9, p. 7, 2009.1472-6882http://hdl.handle.net/11449/737210.1186/1472-6882-9-2WOS:000264034500001WOS000264034500001.pdf0000-0003-3032-2556Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Complementary and Alternative Medicine2.1090,858info:eu-repo/semantics/openAccess2024-06-21T15:18:33Zoai:repositorio.unesp.br:11449/7372Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:05:59.777147Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Anti-Helicobacter pylori activity and immunostimulatory effect of extracts from Byrsonima crassa Nied. (Malpighiaceae) |
title |
Anti-Helicobacter pylori activity and immunostimulatory effect of extracts from Byrsonima crassa Nied. (Malpighiaceae) |
spellingShingle |
Anti-Helicobacter pylori activity and immunostimulatory effect of extracts from Byrsonima crassa Nied. (Malpighiaceae) Bonacorsi, Cibele [UNESP] |
title_short |
Anti-Helicobacter pylori activity and immunostimulatory effect of extracts from Byrsonima crassa Nied. (Malpighiaceae) |
title_full |
Anti-Helicobacter pylori activity and immunostimulatory effect of extracts from Byrsonima crassa Nied. (Malpighiaceae) |
title_fullStr |
Anti-Helicobacter pylori activity and immunostimulatory effect of extracts from Byrsonima crassa Nied. (Malpighiaceae) |
title_full_unstemmed |
Anti-Helicobacter pylori activity and immunostimulatory effect of extracts from Byrsonima crassa Nied. (Malpighiaceae) |
title_sort |
Anti-Helicobacter pylori activity and immunostimulatory effect of extracts from Byrsonima crassa Nied. (Malpighiaceae) |
author |
Bonacorsi, Cibele [UNESP] |
author_facet |
Bonacorsi, Cibele [UNESP] Raddi, Maria Stella G. [UNESP] Carlos, Iracilda Z. [UNESP] Sannomiya, Miriam [UNESP] Vilegas, Wagner [UNESP] |
author_role |
author |
author2 |
Raddi, Maria Stella G. [UNESP] Carlos, Iracilda Z. [UNESP] Sannomiya, Miriam [UNESP] Vilegas, Wagner [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bonacorsi, Cibele [UNESP] Raddi, Maria Stella G. [UNESP] Carlos, Iracilda Z. [UNESP] Sannomiya, Miriam [UNESP] Vilegas, Wagner [UNESP] |
description |
Background: Several in vitro studies have looked at the effect of medicinal plant extracts against Helicobacter pylori (H. pylori). Regardless of the popular use of Byrsonima crassa (B. crassa) as antiemetic, diuretic, febrifuge, to treat diarrhea, gastritis and ulcers, there is no data on its effects against H. pylori. In this study, we evaluated the anti-H. pylori of B. crassa leaves extracts and its effects on reactive oxygen/nitrogen intermediates induction by murine peritoneal macrophages.Methods: The minimal inhibitory concentration (MIC) was determined by broth microdilution method and the production of hydrogen peroxide (H(2)O(2)) and nitric oxide (NO) by the horseradish peroxidase-dependent oxidation of phenol red and Griess reaction, respectively.Results: The methanolic (MeOH) and chloroformic (CHCl(3)) extracts inhibit, in vitro, the growth of H. pylori with MIC value of 1024 mu g/ml. The MeOH extract induced the production H(2)O(2) and NO, but CHCl(3) extract only NO.Conclusion: Based in our results, B. crassa can be considered a source of compounds with anti-H. pylori activity, but its use should be done with caution in treatment of the gastritis and peptic ulcers, since the reactive oxygen/nitrogen intermediates are involved in the pathogenesis of gastric mucosal injury induced by ulcerogenic agents and H. pylori infections. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-16 2014-05-20T13:24:02Z 2014-05-20T13:24:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1472-6882-9-2 Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 9, p. 7, 2009. 1472-6882 http://hdl.handle.net/11449/7372 10.1186/1472-6882-9-2 WOS:000264034500001 WOS000264034500001.pdf 0000-0003-3032-2556 |
url |
http://dx.doi.org/10.1186/1472-6882-9-2 http://hdl.handle.net/11449/7372 |
identifier_str_mv |
Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 9, p. 7, 2009. 1472-6882 10.1186/1472-6882-9-2 WOS:000264034500001 WOS000264034500001.pdf 0000-0003-3032-2556 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Complementary and Alternative Medicine 2.109 0,858 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128461373440000 |