Electrical stimulation enhances early palatal wound healing in mice
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2020.105028 http://hdl.handle.net/11449/208284 |
Resumo: | Background/objective: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. Methods: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 μA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1β, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. Results: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups. Conclusion: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers. |
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Electrical stimulation enhances early palatal wound healing in miceElectrical stimulationGingival recessionHard palateOral mucosaRe-epithelializationWound healingBackground/objective: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. Methods: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 μA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1β, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. Results: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups. Conclusion: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers.Postgraduate Student Oral Biopathology Program São Paulo State University (UNESP) College of Dentistry, Periodontics AreaSão Paulo State University (UNESP) Division of Periodontics Institute of Science and TechnologyUniversity of Campinas (UNICAMP) Department of Prosthodontics and Periodontics Division of Periodontics Piracicaba Dental SchoolFEI – College of Industrial EngineeringUniversity of Michigan School of Dentistry Department of Biologic and Materials Sciences & ProsthodonticsPostgraduate Student Oral Biopathology Program São Paulo State University (UNESP) College of Dentistry, Periodontics AreaSão Paulo State University (UNESP) Division of Periodontics Institute of Science and TechnologyUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)FEI – College of Industrial EngineeringSchool of DentistryFerreira, Camila Lopes [UNESP]Neves Jardini, Maria Aparecida [UNESP]Moretto Nunes, Camilla Magnoni [UNESP]Bernardo, Daniella Vicensotto [UNESP]Viana Casarin, Renato Corrêados Santos Gedraite, EstevãoMathias, Márcio AntônioLiu, FeiMendonça, GustavoSilveira Mendonça, Daniela BaccelliSantamaria, Mauro Pedrine [UNESP]2021-06-25T11:09:39Z2021-06-25T11:09:39Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.archoralbio.2020.105028Archives of Oral Biology, v. 122.1879-15060003-9969http://hdl.handle.net/11449/20828410.1016/j.archoralbio.2020.1050282-s2.0-85098602226Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biologyinfo:eu-repo/semantics/openAccess2021-10-23T18:56:56Zoai:repositorio.unesp.br:11449/208284Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:05:15.993157Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Electrical stimulation enhances early palatal wound healing in mice |
title |
Electrical stimulation enhances early palatal wound healing in mice |
spellingShingle |
Electrical stimulation enhances early palatal wound healing in mice Ferreira, Camila Lopes [UNESP] Electrical stimulation Gingival recession Hard palate Oral mucosa Re-epithelialization Wound healing |
title_short |
Electrical stimulation enhances early palatal wound healing in mice |
title_full |
Electrical stimulation enhances early palatal wound healing in mice |
title_fullStr |
Electrical stimulation enhances early palatal wound healing in mice |
title_full_unstemmed |
Electrical stimulation enhances early palatal wound healing in mice |
title_sort |
Electrical stimulation enhances early palatal wound healing in mice |
author |
Ferreira, Camila Lopes [UNESP] |
author_facet |
Ferreira, Camila Lopes [UNESP] Neves Jardini, Maria Aparecida [UNESP] Moretto Nunes, Camilla Magnoni [UNESP] Bernardo, Daniella Vicensotto [UNESP] Viana Casarin, Renato Corrêa dos Santos Gedraite, Estevão Mathias, Márcio Antônio Liu, Fei Mendonça, Gustavo Silveira Mendonça, Daniela Baccelli Santamaria, Mauro Pedrine [UNESP] |
author_role |
author |
author2 |
Neves Jardini, Maria Aparecida [UNESP] Moretto Nunes, Camilla Magnoni [UNESP] Bernardo, Daniella Vicensotto [UNESP] Viana Casarin, Renato Corrêa dos Santos Gedraite, Estevão Mathias, Márcio Antônio Liu, Fei Mendonça, Gustavo Silveira Mendonça, Daniela Baccelli Santamaria, Mauro Pedrine [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) FEI – College of Industrial Engineering School of Dentistry |
dc.contributor.author.fl_str_mv |
Ferreira, Camila Lopes [UNESP] Neves Jardini, Maria Aparecida [UNESP] Moretto Nunes, Camilla Magnoni [UNESP] Bernardo, Daniella Vicensotto [UNESP] Viana Casarin, Renato Corrêa dos Santos Gedraite, Estevão Mathias, Márcio Antônio Liu, Fei Mendonça, Gustavo Silveira Mendonça, Daniela Baccelli Santamaria, Mauro Pedrine [UNESP] |
dc.subject.por.fl_str_mv |
Electrical stimulation Gingival recession Hard palate Oral mucosa Re-epithelialization Wound healing |
topic |
Electrical stimulation Gingival recession Hard palate Oral mucosa Re-epithelialization Wound healing |
description |
Background/objective: Electrical stimulation (ES) has been used to treat chronic wound and other clinical applications, showing favorable results in wound closure. It was hypothesized that ES can present a positive effect on oral mucosa healing. The aim of this study was to investigate the effects of ES during the palatal mucosa early healing process in Swiss mice. Methods: Ninety animals were divided into two groups: Control (C; n = 45), which received Sham ES applications, and Test (ES; n = 45), which received ES (100 μA; 9 kHz; 660 mVpp) once a day for 3 days. A full thickness wound was performed with a 1.5 mm diameter biopsy punch in the hard palate. Histologically, the following parameters were evaluated: palatal wound closure and epithelial and connective wound edge distance (EED and CED). Furthermore, IL-1β, IL-6, IL-10 TNF-α, and VEGF cytokine levels were evaluated by multiplex assay. The percentage of collagen fibers was assessed using the polarization method and the Smad proteins using the immunofluorescence method. Results: Palatal wound closure presented a significant reduction on day 5 in the ES group (p = 0.01). Additionally, both EED and CED were shorter for all time points in the ES group (p < 0.05), and the inflammatory markers IL-6, IL-10, TNF-α, and VEGF were reduced (p < 0.05). There were no differences in collagen fibers and phospho-Smad2 between the groups. Conclusion: ES had a positive effect on early palatal wound closure outcomes, as well as on inflammatory markers. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:09:39Z 2021-06-25T11:09:39Z 2021-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2020.105028 Archives of Oral Biology, v. 122. 1879-1506 0003-9969 http://hdl.handle.net/11449/208284 10.1016/j.archoralbio.2020.105028 2-s2.0-85098602226 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2020.105028 http://hdl.handle.net/11449/208284 |
identifier_str_mv |
Archives of Oral Biology, v. 122. 1879-1506 0003-9969 10.1016/j.archoralbio.2020.105028 2-s2.0-85098602226 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128892409479168 |