Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses

Detalhes bibliográficos
Autor(a) principal: Poletto, Andréia B. [UNESP]
Data de Publicação: 2008
Outros Autores: Wasko, Adriane Pinto [UNESP], Oliveira, Claudio [UNESP], Azevedo, Alexandre [UNESP], Carvalho, Robson F. [UNESP], Silva, Maeli Dal Pai [UNESP], Foresti, Fausto [UNESP], Martins, Cesar [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1415-47572008000200031
http://hdl.handle.net/11449/130606
Resumo: Actin-encoding cDNAs of Nile tilapia (Oreochromis niloticus) were isolated by RT-PCR using total RNA samples of different tissues and further characterized by nucleotide sequencing and in silico amino acid (aa) sequence analysis. Comparisons among the actin gene sequences of O. niloticus and those of other species evidenced that the isolated genes present a high similarity to other fish and other vertebrate actin genes. The highest nucleotide resemblance was observed between O. niloticus and O. mossambicus a-actin and b-actin genes. Analysis of the predicted aa sequences revealed two distinct types of cytoplasmic actins, one cardiac muscle actin type and one skeletal muscle actin type that were expressed in different tissues of Nile tilapia. The evolutionary relationships between the Nile tilapia actin genes and diverse other organisms is discussed.
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spelling Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analysesactinexpression patternNile tilapiaOreochromis niloticusActin-encoding cDNAs of Nile tilapia (Oreochromis niloticus) were isolated by RT-PCR using total RNA samples of different tissues and further characterized by nucleotide sequencing and in silico amino acid (aa) sequence analysis. Comparisons among the actin gene sequences of O. niloticus and those of other species evidenced that the isolated genes present a high similarity to other fish and other vertebrate actin genes. The highest nucleotide resemblance was observed between O. niloticus and O. mossambicus a-actin and b-actin genes. Analysis of the predicted aa sequences revealed two distinct types of cytoplasmic actins, one cardiac muscle actin type and one skeletal muscle actin type that were expressed in different tissues of Nile tilapia. The evolutionary relationships between the Nile tilapia actin genes and diverse other organisms is discussed.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista Instituto de Biociências Departamento de MorfologiaUniversidade Estadual Paulista Instituto de Biociências Departamento de GenéticaUniversidade Estadual Paulista Instituto de Biociências Departamento de MorfologiaUniversidade Estadual Paulista Instituto de Biociências Departamento de GenéticaSociedade Brasileira de GenéticaUniversidade Estadual Paulista (Unesp)Poletto, Andréia B. [UNESP]Wasko, Adriane Pinto [UNESP]Oliveira, Claudio [UNESP]Azevedo, Alexandre [UNESP]Carvalho, Robson F. [UNESP]Silva, Maeli Dal Pai [UNESP]Foresti, Fausto [UNESP]Martins, Cesar [UNESP]2014-05-20T13:51:44Z2014-05-20T13:51:44Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article325-356application/pdfhttp://dx.doi.org/10.1590/S1415-47572008000200031Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 31, n. 1, p. 325-356, 2008.1415-4757http://hdl.handle.net/11449/13060610.1590/S1415-47572008000200031S1415-47572008000200031WOS:000257238500031S1415-47572008000200031.pdf1926863917378909080479394484636788588006994253520000-0002-4901-77140000-0003-4600-03670000-0003-3534-974XSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-12-07T06:21:17Zoai:repositorio.unesp.br:11449/130606Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:44:30.602232Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses
title Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses
spellingShingle Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses
Poletto, Andréia B. [UNESP]
actin
expression pattern
Nile tilapia
Oreochromis niloticus
title_short Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses
title_full Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses
title_fullStr Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses
title_full_unstemmed Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses
title_sort Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses
author Poletto, Andréia B. [UNESP]
author_facet Poletto, Andréia B. [UNESP]
Wasko, Adriane Pinto [UNESP]
Oliveira, Claudio [UNESP]
Azevedo, Alexandre [UNESP]
Carvalho, Robson F. [UNESP]
Silva, Maeli Dal Pai [UNESP]
Foresti, Fausto [UNESP]
Martins, Cesar [UNESP]
author_role author
author2 Wasko, Adriane Pinto [UNESP]
Oliveira, Claudio [UNESP]
Azevedo, Alexandre [UNESP]
Carvalho, Robson F. [UNESP]
Silva, Maeli Dal Pai [UNESP]
Foresti, Fausto [UNESP]
Martins, Cesar [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Poletto, Andréia B. [UNESP]
Wasko, Adriane Pinto [UNESP]
Oliveira, Claudio [UNESP]
Azevedo, Alexandre [UNESP]
Carvalho, Robson F. [UNESP]
Silva, Maeli Dal Pai [UNESP]
Foresti, Fausto [UNESP]
Martins, Cesar [UNESP]
dc.subject.por.fl_str_mv actin
expression pattern
Nile tilapia
Oreochromis niloticus
topic actin
expression pattern
Nile tilapia
Oreochromis niloticus
description Actin-encoding cDNAs of Nile tilapia (Oreochromis niloticus) were isolated by RT-PCR using total RNA samples of different tissues and further characterized by nucleotide sequencing and in silico amino acid (aa) sequence analysis. Comparisons among the actin gene sequences of O. niloticus and those of other species evidenced that the isolated genes present a high similarity to other fish and other vertebrate actin genes. The highest nucleotide resemblance was observed between O. niloticus and O. mossambicus a-actin and b-actin genes. Analysis of the predicted aa sequences revealed two distinct types of cytoplasmic actins, one cardiac muscle actin type and one skeletal muscle actin type that were expressed in different tissues of Nile tilapia. The evolutionary relationships between the Nile tilapia actin genes and diverse other organisms is discussed.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
2014-05-20T13:51:44Z
2014-05-20T13:51:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572008000200031
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 31, n. 1, p. 325-356, 2008.
1415-4757
http://hdl.handle.net/11449/130606
10.1590/S1415-47572008000200031
S1415-47572008000200031
WOS:000257238500031
S1415-47572008000200031.pdf
1926863917378909
0804793944846367
8858800699425352
0000-0002-4901-7714
0000-0003-4600-0367
0000-0003-3534-974X
url http://dx.doi.org/10.1590/S1415-47572008000200031
http://hdl.handle.net/11449/130606
identifier_str_mv Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 31, n. 1, p. 325-356, 2008.
1415-4757
10.1590/S1415-47572008000200031
S1415-47572008000200031
WOS:000257238500031
S1415-47572008000200031.pdf
1926863917378909
0804793944846367
8858800699425352
0000-0002-4901-7714
0000-0003-4600-0367
0000-0003-3534-974X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Biology
1.493
0,638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 325-356
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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