Anticancer effects of geopropolis produced by stingless bees on canine osteosarcoma cells in vitro
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2013/737386 http://hdl.handle.net/11449/75387 |
Resumo: | Geopropolis is produced by indigenous stingless bees from the resinous material of plants, adding soil or clay. Its biological properties have not been investigated, such as propolis, and herein its cytotoxic action on canine osteosarcoma (OSA) cells was evaluated. OSA is a primary bone neoplasm diagnosed in dogs being an excellent model in vivo to study human OSA. spOS-2 primary cultures were isolated from the tumor of a dog with osteosarcoma and incubated with geopropolis, 70% ethanol (geopropolis solvent), and carboplatin after 6, 24, 48, and 72 hours. Cell viability was analyzed by the crystal violet method. Geopropolis was efficient against canine OSA cells in a dose- and time-dependent way, leading to a distinct morphology compared to control. Geopropolis cytotoxic action was exclusively due to its constituents since 70% ethanol (its solvent) had no effect on cell viability. Carboplatin had no effect on OSA cells. Geopropolis exerted a cytotoxic effect on canine osteosarcoma, and its introduction as a possible therapeutic agent in vivo could be investigated, providing a new contribution to OSA treatment. © 2013 Naiara Costa Cinegaglia et al. |
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Anticancer effects of geopropolis produced by stingless bees on canine osteosarcoma cells in vitroalcoholantineoplastic agentcarboplatincrystal violetgeopropolispropolissolventunclassified druganimal cellanimal experimentanimal modelanimal tissueantineoplastic activitybeebone cancercancer cellcancer cell culturecell structurecell viabilitycontrolled studydog diseasedrug cytotoxicityin vitro studyin vivo studynonhumanosteosarcomapriority journalGeopropolis is produced by indigenous stingless bees from the resinous material of plants, adding soil or clay. Its biological properties have not been investigated, such as propolis, and herein its cytotoxic action on canine osteosarcoma (OSA) cells was evaluated. OSA is a primary bone neoplasm diagnosed in dogs being an excellent model in vivo to study human OSA. spOS-2 primary cultures were isolated from the tumor of a dog with osteosarcoma and incubated with geopropolis, 70% ethanol (geopropolis solvent), and carboplatin after 6, 24, 48, and 72 hours. Cell viability was analyzed by the crystal violet method. Geopropolis was efficient against canine OSA cells in a dose- and time-dependent way, leading to a distinct morphology compared to control. Geopropolis cytotoxic action was exclusively due to its constituents since 70% ethanol (its solvent) had no effect on cell viability. Carboplatin had no effect on OSA cells. Geopropolis exerted a cytotoxic effect on canine osteosarcoma, and its introduction as a possible therapeutic agent in vivo could be investigated, providing a new contribution to OSA treatment. © 2013 Naiara Costa Cinegaglia et al.Surgery and Orthopedics Department Medical School São Paulo State University (UNESP), 18618-970 Botucatu, SPInvestigative and Comparative Pathology Laboratory College of Veterinary Medicine and Animal Husbandry São Paulo State University (UNESP), 18618-970 Botucatu, SPDepartment of Microbiology and Immunology Biosciences Institute São Paulo State University (UNESP), 18618-970 Botucatu, SPSurgery and Orthopedics Department Medical School São Paulo State University (UNESP), 18618-970 Botucatu, SPInvestigative and Comparative Pathology Laboratory College of Veterinary Medicine and Animal Husbandry São Paulo State University (UNESP), 18618-970 Botucatu, SPDepartment of Microbiology and Immunology Biosciences Institute São Paulo State University (UNESP), 18618-970 Botucatu, SPUniversidade Estadual Paulista (Unesp)Cinegaglia, Naiara Costa [UNESP]Bersano, Paulo Ricardo Oliveira [UNESP]Araújo, Maria José Abigail Mendes [UNESP]Búfalo, Michelle Cristiane [UNESP]Sforcin, José Mauricio [UNESP]2014-05-27T11:29:29Z2014-05-27T11:29:29Z2013-05-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2013/737386Evidence-based Complementary and Alternative Medicine, v. 2013.1741-427X1741-4288http://hdl.handle.net/11449/7538710.1155/2013/737386WOS:0003180960000012-s2.0-848772491242-s2.0-84877249124.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEvidence-based Complementary and Alternative Medicine2.0640,683info:eu-repo/semantics/openAccess2024-01-12T06:22:15Zoai:repositorio.unesp.br:11449/75387Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-12T06:22:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Anticancer effects of geopropolis produced by stingless bees on canine osteosarcoma cells in vitro |
title |
Anticancer effects of geopropolis produced by stingless bees on canine osteosarcoma cells in vitro |
spellingShingle |
Anticancer effects of geopropolis produced by stingless bees on canine osteosarcoma cells in vitro Cinegaglia, Naiara Costa [UNESP] alcohol antineoplastic agent carboplatin crystal violet geopropolis propolis solvent unclassified drug animal cell animal experiment animal model animal tissue antineoplastic activity bee bone cancer cancer cell cancer cell culture cell structure cell viability controlled study dog disease drug cytotoxicity in vitro study in vivo study nonhuman osteosarcoma priority journal |
title_short |
Anticancer effects of geopropolis produced by stingless bees on canine osteosarcoma cells in vitro |
title_full |
Anticancer effects of geopropolis produced by stingless bees on canine osteosarcoma cells in vitro |
title_fullStr |
Anticancer effects of geopropolis produced by stingless bees on canine osteosarcoma cells in vitro |
title_full_unstemmed |
Anticancer effects of geopropolis produced by stingless bees on canine osteosarcoma cells in vitro |
title_sort |
Anticancer effects of geopropolis produced by stingless bees on canine osteosarcoma cells in vitro |
author |
Cinegaglia, Naiara Costa [UNESP] |
author_facet |
Cinegaglia, Naiara Costa [UNESP] Bersano, Paulo Ricardo Oliveira [UNESP] Araújo, Maria José Abigail Mendes [UNESP] Búfalo, Michelle Cristiane [UNESP] Sforcin, José Mauricio [UNESP] |
author_role |
author |
author2 |
Bersano, Paulo Ricardo Oliveira [UNESP] Araújo, Maria José Abigail Mendes [UNESP] Búfalo, Michelle Cristiane [UNESP] Sforcin, José Mauricio [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cinegaglia, Naiara Costa [UNESP] Bersano, Paulo Ricardo Oliveira [UNESP] Araújo, Maria José Abigail Mendes [UNESP] Búfalo, Michelle Cristiane [UNESP] Sforcin, José Mauricio [UNESP] |
dc.subject.por.fl_str_mv |
alcohol antineoplastic agent carboplatin crystal violet geopropolis propolis solvent unclassified drug animal cell animal experiment animal model animal tissue antineoplastic activity bee bone cancer cancer cell cancer cell culture cell structure cell viability controlled study dog disease drug cytotoxicity in vitro study in vivo study nonhuman osteosarcoma priority journal |
topic |
alcohol antineoplastic agent carboplatin crystal violet geopropolis propolis solvent unclassified drug animal cell animal experiment animal model animal tissue antineoplastic activity bee bone cancer cancer cell cancer cell culture cell structure cell viability controlled study dog disease drug cytotoxicity in vitro study in vivo study nonhuman osteosarcoma priority journal |
description |
Geopropolis is produced by indigenous stingless bees from the resinous material of plants, adding soil or clay. Its biological properties have not been investigated, such as propolis, and herein its cytotoxic action on canine osteosarcoma (OSA) cells was evaluated. OSA is a primary bone neoplasm diagnosed in dogs being an excellent model in vivo to study human OSA. spOS-2 primary cultures were isolated from the tumor of a dog with osteosarcoma and incubated with geopropolis, 70% ethanol (geopropolis solvent), and carboplatin after 6, 24, 48, and 72 hours. Cell viability was analyzed by the crystal violet method. Geopropolis was efficient against canine OSA cells in a dose- and time-dependent way, leading to a distinct morphology compared to control. Geopropolis cytotoxic action was exclusively due to its constituents since 70% ethanol (its solvent) had no effect on cell viability. Carboplatin had no effect on OSA cells. Geopropolis exerted a cytotoxic effect on canine osteosarcoma, and its introduction as a possible therapeutic agent in vivo could be investigated, providing a new contribution to OSA treatment. © 2013 Naiara Costa Cinegaglia et al. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05-13 2014-05-27T11:29:29Z 2014-05-27T11:29:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2013/737386 Evidence-based Complementary and Alternative Medicine, v. 2013. 1741-427X 1741-4288 http://hdl.handle.net/11449/75387 10.1155/2013/737386 WOS:000318096000001 2-s2.0-84877249124 2-s2.0-84877249124.pdf |
url |
http://dx.doi.org/10.1155/2013/737386 http://hdl.handle.net/11449/75387 |
identifier_str_mv |
Evidence-based Complementary and Alternative Medicine, v. 2013. 1741-427X 1741-4288 10.1155/2013/737386 WOS:000318096000001 2-s2.0-84877249124 2-s2.0-84877249124.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Evidence-based Complementary and Alternative Medicine 2.064 0,683 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965591332716544 |