Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals

Detalhes bibliográficos
Autor(a) principal: Pires, Regina Helena [UNESP]
Data de Publicação: 2011
Outros Autores: dos Santos, Jaime Maia [UNESP], Zaia, José Eduardo, Martins, Carlos Henrique Gomes, Mendes-Giannini, Maria José Soares [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0074-02762011000600002
http://hdl.handle.net/11449/226561
Resumo: Candida parapsilosis, currently divided into three distinct species, proliferates in glucose-rich solutions and has been associated with infections resulting from the use of medical devices made of plastic, an environment common in dialysis centres. The aims of this study were (i) to screen for Candida orthopsilosis and Candida metapsilosis (100 environmental isolates previously identified as C. parapsilosis), (ii) to test the ability of these isolates to form biofilm and (iii) to investigate the in vitro susceptibility of Candida spp biofilms to the antifungal agents, fluconazole (FLC) and amphotericin B (AMB). Isolates were obtained from a hydraulic circuit collected from a haemodialysis unit. Based on molecular criteria, 47 strains were re-identified as C. orthopsilosis and 53 as C. parapsilosis. Analyses using a formazan salt reduction assay and total viable count, together with microscopy studies, revealed that 72 strains were able to form biofilm that was structurally similar, but with minor differences in morphology. A microtitre-based colorimetric assay used to test the susceptibility of fungal biofilms to AMB and FLC demonstrated that the C. parapsilosis complex displayed an increased resistance to these antifungal agents. The results from these analyses may provide a basis for implementing quality controls and monitoring to ensure the microbiological purity of dialysis water, including the presence of yeast.
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spelling Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungalsBiofilmCandida orthopsilosisCandida parapsilosisHaemodialysis solutionCandida parapsilosis, currently divided into three distinct species, proliferates in glucose-rich solutions and has been associated with infections resulting from the use of medical devices made of plastic, an environment common in dialysis centres. The aims of this study were (i) to screen for Candida orthopsilosis and Candida metapsilosis (100 environmental isolates previously identified as C. parapsilosis), (ii) to test the ability of these isolates to form biofilm and (iii) to investigate the in vitro susceptibility of Candida spp biofilms to the antifungal agents, fluconazole (FLC) and amphotericin B (AMB). Isolates were obtained from a hydraulic circuit collected from a haemodialysis unit. Based on molecular criteria, 47 strains were re-identified as C. orthopsilosis and 53 as C. parapsilosis. Analyses using a formazan salt reduction assay and total viable count, together with microscopy studies, revealed that 72 strains were able to form biofilm that was structurally similar, but with minor differences in morphology. A microtitre-based colorimetric assay used to test the susceptibility of fungal biofilms to AMB and FLC demonstrated that the C. parapsilosis complex displayed an increased resistance to these antifungal agents. The results from these analyses may provide a basis for implementing quality controls and monitoring to ensure the microbiological purity of dialysis water, including the presence of yeast.Departamento de Análises Clínicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, Araraquara, SPDepartamento de Fitossanidade Vegetal Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, Jaboticabal, SPLaboratório de Pesquisa em Microbiologia Aplicada Universidade de Franca, Franca, SPDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, Araraquara, SPDepartamento de Fitossanidade Vegetal Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, Jaboticabal, SPUniversidade Estadual Paulista (UNESP)Universidade de FrancaPires, Regina Helena [UNESP]dos Santos, Jaime Maia [UNESP]Zaia, José EduardoMartins, Carlos Henrique GomesMendes-Giannini, Maria José Soares [UNESP]2022-04-29T01:14:05Z2022-04-29T01:14:05Z2011-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article646-654http://dx.doi.org/10.1590/S0074-02762011000600002Memorias do Instituto Oswaldo Cruz, v. 106, n. 6, p. 646-654, 2011.1678-80600074-0276http://hdl.handle.net/11449/22656110.1590/S0074-027620110006000022-s2.0-80053996906Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemorias do Instituto Oswaldo Cruzinfo:eu-repo/semantics/openAccess2022-04-29T01:14:05Zoai:repositorio.unesp.br:11449/226561Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T01:14:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals
title Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals
spellingShingle Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals
Pires, Regina Helena [UNESP]
Biofilm
Candida orthopsilosis
Candida parapsilosis
Haemodialysis solution
title_short Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals
title_full Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals
title_fullStr Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals
title_full_unstemmed Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals
title_sort Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals
author Pires, Regina Helena [UNESP]
author_facet Pires, Regina Helena [UNESP]
dos Santos, Jaime Maia [UNESP]
Zaia, José Eduardo
Martins, Carlos Henrique Gomes
Mendes-Giannini, Maria José Soares [UNESP]
author_role author
author2 dos Santos, Jaime Maia [UNESP]
Zaia, José Eduardo
Martins, Carlos Henrique Gomes
Mendes-Giannini, Maria José Soares [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade de Franca
dc.contributor.author.fl_str_mv Pires, Regina Helena [UNESP]
dos Santos, Jaime Maia [UNESP]
Zaia, José Eduardo
Martins, Carlos Henrique Gomes
Mendes-Giannini, Maria José Soares [UNESP]
dc.subject.por.fl_str_mv Biofilm
Candida orthopsilosis
Candida parapsilosis
Haemodialysis solution
topic Biofilm
Candida orthopsilosis
Candida parapsilosis
Haemodialysis solution
description Candida parapsilosis, currently divided into three distinct species, proliferates in glucose-rich solutions and has been associated with infections resulting from the use of medical devices made of plastic, an environment common in dialysis centres. The aims of this study were (i) to screen for Candida orthopsilosis and Candida metapsilosis (100 environmental isolates previously identified as C. parapsilosis), (ii) to test the ability of these isolates to form biofilm and (iii) to investigate the in vitro susceptibility of Candida spp biofilms to the antifungal agents, fluconazole (FLC) and amphotericin B (AMB). Isolates were obtained from a hydraulic circuit collected from a haemodialysis unit. Based on molecular criteria, 47 strains were re-identified as C. orthopsilosis and 53 as C. parapsilosis. Analyses using a formazan salt reduction assay and total viable count, together with microscopy studies, revealed that 72 strains were able to form biofilm that was structurally similar, but with minor differences in morphology. A microtitre-based colorimetric assay used to test the susceptibility of fungal biofilms to AMB and FLC demonstrated that the C. parapsilosis complex displayed an increased resistance to these antifungal agents. The results from these analyses may provide a basis for implementing quality controls and monitoring to ensure the microbiological purity of dialysis water, including the presence of yeast.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
2022-04-29T01:14:05Z
2022-04-29T01:14:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0074-02762011000600002
Memorias do Instituto Oswaldo Cruz, v. 106, n. 6, p. 646-654, 2011.
1678-8060
0074-0276
http://hdl.handle.net/11449/226561
10.1590/S0074-02762011000600002
2-s2.0-80053996906
url http://dx.doi.org/10.1590/S0074-02762011000600002
http://hdl.handle.net/11449/226561
identifier_str_mv Memorias do Instituto Oswaldo Cruz, v. 106, n. 6, p. 646-654, 2011.
1678-8060
0074-0276
10.1590/S0074-02762011000600002
2-s2.0-80053996906
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memorias do Instituto Oswaldo Cruz
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 646-654
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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