Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0074-02762011000600002 http://hdl.handle.net/11449/226561 |
Resumo: | Candida parapsilosis, currently divided into three distinct species, proliferates in glucose-rich solutions and has been associated with infections resulting from the use of medical devices made of plastic, an environment common in dialysis centres. The aims of this study were (i) to screen for Candida orthopsilosis and Candida metapsilosis (100 environmental isolates previously identified as C. parapsilosis), (ii) to test the ability of these isolates to form biofilm and (iii) to investigate the in vitro susceptibility of Candida spp biofilms to the antifungal agents, fluconazole (FLC) and amphotericin B (AMB). Isolates were obtained from a hydraulic circuit collected from a haemodialysis unit. Based on molecular criteria, 47 strains were re-identified as C. orthopsilosis and 53 as C. parapsilosis. Analyses using a formazan salt reduction assay and total viable count, together with microscopy studies, revealed that 72 strains were able to form biofilm that was structurally similar, but with minor differences in morphology. A microtitre-based colorimetric assay used to test the susceptibility of fungal biofilms to AMB and FLC demonstrated that the C. parapsilosis complex displayed an increased resistance to these antifungal agents. The results from these analyses may provide a basis for implementing quality controls and monitoring to ensure the microbiological purity of dialysis water, including the presence of yeast. |
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Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungalsBiofilmCandida orthopsilosisCandida parapsilosisHaemodialysis solutionCandida parapsilosis, currently divided into three distinct species, proliferates in glucose-rich solutions and has been associated with infections resulting from the use of medical devices made of plastic, an environment common in dialysis centres. The aims of this study were (i) to screen for Candida orthopsilosis and Candida metapsilosis (100 environmental isolates previously identified as C. parapsilosis), (ii) to test the ability of these isolates to form biofilm and (iii) to investigate the in vitro susceptibility of Candida spp biofilms to the antifungal agents, fluconazole (FLC) and amphotericin B (AMB). Isolates were obtained from a hydraulic circuit collected from a haemodialysis unit. Based on molecular criteria, 47 strains were re-identified as C. orthopsilosis and 53 as C. parapsilosis. Analyses using a formazan salt reduction assay and total viable count, together with microscopy studies, revealed that 72 strains were able to form biofilm that was structurally similar, but with minor differences in morphology. A microtitre-based colorimetric assay used to test the susceptibility of fungal biofilms to AMB and FLC demonstrated that the C. parapsilosis complex displayed an increased resistance to these antifungal agents. The results from these analyses may provide a basis for implementing quality controls and monitoring to ensure the microbiological purity of dialysis water, including the presence of yeast.Departamento de Análises Clínicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, Araraquara, SPDepartamento de Fitossanidade Vegetal Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, Jaboticabal, SPLaboratório de Pesquisa em Microbiologia Aplicada Universidade de Franca, Franca, SPDepartamento de Análises Clínicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, Araraquara, SPDepartamento de Fitossanidade Vegetal Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, Jaboticabal, SPUniversidade Estadual Paulista (UNESP)Universidade de FrancaPires, Regina Helena [UNESP]dos Santos, Jaime Maia [UNESP]Zaia, José EduardoMartins, Carlos Henrique GomesMendes-Giannini, Maria José Soares [UNESP]2022-04-29T01:14:05Z2022-04-29T01:14:05Z2011-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article646-654http://dx.doi.org/10.1590/S0074-02762011000600002Memorias do Instituto Oswaldo Cruz, v. 106, n. 6, p. 646-654, 2011.1678-80600074-0276http://hdl.handle.net/11449/22656110.1590/S0074-027620110006000022-s2.0-80053996906Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemorias do Instituto Oswaldo Cruzinfo:eu-repo/semantics/openAccess2022-04-29T01:14:05Zoai:repositorio.unesp.br:11449/226561Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T01:14:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals |
title |
Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals |
spellingShingle |
Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals Pires, Regina Helena [UNESP] Biofilm Candida orthopsilosis Candida parapsilosis Haemodialysis solution |
title_short |
Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals |
title_full |
Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals |
title_fullStr |
Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals |
title_full_unstemmed |
Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals |
title_sort |
Candida parapsilosis complex water isolates from a haemodialysis unit: Biofilm production and in vitro evaluation of the use of clinical antifungals |
author |
Pires, Regina Helena [UNESP] |
author_facet |
Pires, Regina Helena [UNESP] dos Santos, Jaime Maia [UNESP] Zaia, José Eduardo Martins, Carlos Henrique Gomes Mendes-Giannini, Maria José Soares [UNESP] |
author_role |
author |
author2 |
dos Santos, Jaime Maia [UNESP] Zaia, José Eduardo Martins, Carlos Henrique Gomes Mendes-Giannini, Maria José Soares [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de Franca |
dc.contributor.author.fl_str_mv |
Pires, Regina Helena [UNESP] dos Santos, Jaime Maia [UNESP] Zaia, José Eduardo Martins, Carlos Henrique Gomes Mendes-Giannini, Maria José Soares [UNESP] |
dc.subject.por.fl_str_mv |
Biofilm Candida orthopsilosis Candida parapsilosis Haemodialysis solution |
topic |
Biofilm Candida orthopsilosis Candida parapsilosis Haemodialysis solution |
description |
Candida parapsilosis, currently divided into three distinct species, proliferates in glucose-rich solutions and has been associated with infections resulting from the use of medical devices made of plastic, an environment common in dialysis centres. The aims of this study were (i) to screen for Candida orthopsilosis and Candida metapsilosis (100 environmental isolates previously identified as C. parapsilosis), (ii) to test the ability of these isolates to form biofilm and (iii) to investigate the in vitro susceptibility of Candida spp biofilms to the antifungal agents, fluconazole (FLC) and amphotericin B (AMB). Isolates were obtained from a hydraulic circuit collected from a haemodialysis unit. Based on molecular criteria, 47 strains were re-identified as C. orthopsilosis and 53 as C. parapsilosis. Analyses using a formazan salt reduction assay and total viable count, together with microscopy studies, revealed that 72 strains were able to form biofilm that was structurally similar, but with minor differences in morphology. A microtitre-based colorimetric assay used to test the susceptibility of fungal biofilms to AMB and FLC demonstrated that the C. parapsilosis complex displayed an increased resistance to these antifungal agents. The results from these analyses may provide a basis for implementing quality controls and monitoring to ensure the microbiological purity of dialysis water, including the presence of yeast. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 2022-04-29T01:14:05Z 2022-04-29T01:14:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762011000600002 Memorias do Instituto Oswaldo Cruz, v. 106, n. 6, p. 646-654, 2011. 1678-8060 0074-0276 http://hdl.handle.net/11449/226561 10.1590/S0074-02762011000600002 2-s2.0-80053996906 |
url |
http://dx.doi.org/10.1590/S0074-02762011000600002 http://hdl.handle.net/11449/226561 |
identifier_str_mv |
Memorias do Instituto Oswaldo Cruz, v. 106, n. 6, p. 646-654, 2011. 1678-8060 0074-0276 10.1590/S0074-02762011000600002 2-s2.0-80053996906 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memorias do Instituto Oswaldo Cruz |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
646-654 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965018556465152 |