Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Ana Carolina Siqueira [UNESP]
Data de Publicação: 2018
Outros Autores: Roma Júnior, Luiz Carlos, Privatti, Rafaela Torrezan, Salles, Márcia Saladini Vieira, De Paz, Claudia Cristina Paro, Zadra, Lenira El Faro, Vidal, Ana Maria Centola
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0103-8478cr20170848
http://hdl.handle.net/11449/171181
Resumo: The study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divided into variables related to the equipment: repeatability and reproducibility, and variables that could influencing the results as: use of preservatives, temperature, time between collection and analysis, breed and milk composition, besides analysis to relate the Ekomilk Scan® with the standard method-direct microscopy and reference-flow cytometry. As the result, for samples analysis, it shouldn’t be added preservative and these should be conducted on the same day of collection; however, temperature sample did not significantly influence results. Furthermore, Ekomilk Scan® did not show good correlation of results with the method of direct microscopy; however, it was necessary to generate equations for a positive correlation between flow cytometry and Ekomilk Scan®. Therefore, it is concluded that the equipment tested is not accurate but it can be an alternative for SCC monitoring in productive units since it uses calibration equations of results.
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spelling Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysisContagem de células somáticas obtidas pelo equipamento Ekomilk Scan® e suas correlações com outros métodos de análiseAlternative methodMonitoringSomatic cell countUdder healthThe study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divided into variables related to the equipment: repeatability and reproducibility, and variables that could influencing the results as: use of preservatives, temperature, time between collection and analysis, breed and milk composition, besides analysis to relate the Ekomilk Scan® with the standard method-direct microscopy and reference-flow cytometry. As the result, for samples analysis, it shouldn’t be added preservative and these should be conducted on the same day of collection; however, temperature sample did not significantly influence results. Furthermore, Ekomilk Scan® did not show good correlation of results with the method of direct microscopy; however, it was necessary to generate equations for a positive correlation between flow cytometry and Ekomilk Scan®. Therefore, it is concluded that the equipment tested is not accurate but it can be an alternative for SCC monitoring in productive units since it uses calibration equations of results.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESPInstituto de ZootecniaDepartamento de Engenharia de Alimentos Faculdade de Zootecnia e Engenharia de Alimentos Universidade de São Paulo (USP)Departamento de Medicina Veterinária Faculdade de Zootecnia e Engenharia de Alimentos Universidade de São Paulo (USP)Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESPUniversidade Estadual Paulista (Unesp)Instituto de ZootecniaUniversidade de São Paulo (USP)Gonçalves, Ana Carolina Siqueira [UNESP]Roma Júnior, Luiz CarlosPrivatti, Rafaela TorrezanSalles, Márcia Saladini VieiraDe Paz, Claudia Cristina ParoZadra, Lenira El FaroVidal, Ana Maria Centola2018-12-11T16:54:16Z2018-12-11T16:54:16Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/0103-8478cr20170848Ciencia Rural, v. 48, n. 6, 2018.1678-45960103-8478http://hdl.handle.net/11449/17118110.1590/0103-8478cr20170848S0103-847820180006004542-s2.0-85049506002S0103-84782018000600454.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCiencia Rural0,337info:eu-repo/semantics/openAccess2023-10-19T06:06:53Zoai:repositorio.unesp.br:11449/171181Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-19T06:06:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis
Contagem de células somáticas obtidas pelo equipamento Ekomilk Scan® e suas correlações com outros métodos de análise
title Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis
spellingShingle Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis
Gonçalves, Ana Carolina Siqueira [UNESP]
Alternative method
Monitoring
Somatic cell count
Udder health
title_short Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis
title_full Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis
title_fullStr Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis
title_full_unstemmed Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis
title_sort Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis
author Gonçalves, Ana Carolina Siqueira [UNESP]
author_facet Gonçalves, Ana Carolina Siqueira [UNESP]
Roma Júnior, Luiz Carlos
Privatti, Rafaela Torrezan
Salles, Márcia Saladini Vieira
De Paz, Claudia Cristina Paro
Zadra, Lenira El Faro
Vidal, Ana Maria Centola
author_role author
author2 Roma Júnior, Luiz Carlos
Privatti, Rafaela Torrezan
Salles, Márcia Saladini Vieira
De Paz, Claudia Cristina Paro
Zadra, Lenira El Faro
Vidal, Ana Maria Centola
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto de Zootecnia
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Gonçalves, Ana Carolina Siqueira [UNESP]
Roma Júnior, Luiz Carlos
Privatti, Rafaela Torrezan
Salles, Márcia Saladini Vieira
De Paz, Claudia Cristina Paro
Zadra, Lenira El Faro
Vidal, Ana Maria Centola
dc.subject.por.fl_str_mv Alternative method
Monitoring
Somatic cell count
Udder health
topic Alternative method
Monitoring
Somatic cell count
Udder health
description The study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divided into variables related to the equipment: repeatability and reproducibility, and variables that could influencing the results as: use of preservatives, temperature, time between collection and analysis, breed and milk composition, besides analysis to relate the Ekomilk Scan® with the standard method-direct microscopy and reference-flow cytometry. As the result, for samples analysis, it shouldn’t be added preservative and these should be conducted on the same day of collection; however, temperature sample did not significantly influence results. Furthermore, Ekomilk Scan® did not show good correlation of results with the method of direct microscopy; however, it was necessary to generate equations for a positive correlation between flow cytometry and Ekomilk Scan®. Therefore, it is concluded that the equipment tested is not accurate but it can be an alternative for SCC monitoring in productive units since it uses calibration equations of results.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T16:54:16Z
2018-12-11T16:54:16Z
2018-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0103-8478cr20170848
Ciencia Rural, v. 48, n. 6, 2018.
1678-4596
0103-8478
http://hdl.handle.net/11449/171181
10.1590/0103-8478cr20170848
S0103-84782018000600454
2-s2.0-85049506002
S0103-84782018000600454.pdf
url http://dx.doi.org/10.1590/0103-8478cr20170848
http://hdl.handle.net/11449/171181
identifier_str_mv Ciencia Rural, v. 48, n. 6, 2018.
1678-4596
0103-8478
10.1590/0103-8478cr20170848
S0103-84782018000600454
2-s2.0-85049506002
S0103-84782018000600454.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ciencia Rural
0,337
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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