Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0103-8478cr20170848 http://hdl.handle.net/11449/171181 |
Resumo: | The study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divided into variables related to the equipment: repeatability and reproducibility, and variables that could influencing the results as: use of preservatives, temperature, time between collection and analysis, breed and milk composition, besides analysis to relate the Ekomilk Scan® with the standard method-direct microscopy and reference-flow cytometry. As the result, for samples analysis, it shouldn’t be added preservative and these should be conducted on the same day of collection; however, temperature sample did not significantly influence results. Furthermore, Ekomilk Scan® did not show good correlation of results with the method of direct microscopy; however, it was necessary to generate equations for a positive correlation between flow cytometry and Ekomilk Scan®. Therefore, it is concluded that the equipment tested is not accurate but it can be an alternative for SCC monitoring in productive units since it uses calibration equations of results. |
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Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysisContagem de células somáticas obtidas pelo equipamento Ekomilk Scan® e suas correlações com outros métodos de análiseAlternative methodMonitoringSomatic cell countUdder healthThe study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divided into variables related to the equipment: repeatability and reproducibility, and variables that could influencing the results as: use of preservatives, temperature, time between collection and analysis, breed and milk composition, besides analysis to relate the Ekomilk Scan® with the standard method-direct microscopy and reference-flow cytometry. As the result, for samples analysis, it shouldn’t be added preservative and these should be conducted on the same day of collection; however, temperature sample did not significantly influence results. Furthermore, Ekomilk Scan® did not show good correlation of results with the method of direct microscopy; however, it was necessary to generate equations for a positive correlation between flow cytometry and Ekomilk Scan®. Therefore, it is concluded that the equipment tested is not accurate but it can be an alternative for SCC monitoring in productive units since it uses calibration equations of results.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESPInstituto de ZootecniaDepartamento de Engenharia de Alimentos Faculdade de Zootecnia e Engenharia de Alimentos Universidade de São Paulo (USP)Departamento de Medicina Veterinária Faculdade de Zootecnia e Engenharia de Alimentos Universidade de São Paulo (USP)Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESPUniversidade Estadual Paulista (Unesp)Instituto de ZootecniaUniversidade de São Paulo (USP)Gonçalves, Ana Carolina Siqueira [UNESP]Roma Júnior, Luiz CarlosPrivatti, Rafaela TorrezanSalles, Márcia Saladini VieiraDe Paz, Claudia Cristina ParoZadra, Lenira El FaroVidal, Ana Maria Centola2018-12-11T16:54:16Z2018-12-11T16:54:16Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/0103-8478cr20170848Ciencia Rural, v. 48, n. 6, 2018.1678-45960103-8478http://hdl.handle.net/11449/17118110.1590/0103-8478cr20170848S0103-847820180006004542-s2.0-85049506002S0103-84782018000600454.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCiencia Rural0,337info:eu-repo/semantics/openAccess2023-10-19T06:06:53Zoai:repositorio.unesp.br:11449/171181Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:20:49.634517Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis Contagem de células somáticas obtidas pelo equipamento Ekomilk Scan® e suas correlações com outros métodos de análise |
title |
Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis |
spellingShingle |
Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis Gonçalves, Ana Carolina Siqueira [UNESP] Alternative method Monitoring Somatic cell count Udder health |
title_short |
Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis |
title_full |
Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis |
title_fullStr |
Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis |
title_full_unstemmed |
Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis |
title_sort |
Somatic cell count obtained by ekomilk scan® and correlations with other methods of analysis |
author |
Gonçalves, Ana Carolina Siqueira [UNESP] |
author_facet |
Gonçalves, Ana Carolina Siqueira [UNESP] Roma Júnior, Luiz Carlos Privatti, Rafaela Torrezan Salles, Márcia Saladini Vieira De Paz, Claudia Cristina Paro Zadra, Lenira El Faro Vidal, Ana Maria Centola |
author_role |
author |
author2 |
Roma Júnior, Luiz Carlos Privatti, Rafaela Torrezan Salles, Márcia Saladini Vieira De Paz, Claudia Cristina Paro Zadra, Lenira El Faro Vidal, Ana Maria Centola |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Instituto de Zootecnia Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Gonçalves, Ana Carolina Siqueira [UNESP] Roma Júnior, Luiz Carlos Privatti, Rafaela Torrezan Salles, Márcia Saladini Vieira De Paz, Claudia Cristina Paro Zadra, Lenira El Faro Vidal, Ana Maria Centola |
dc.subject.por.fl_str_mv |
Alternative method Monitoring Somatic cell count Udder health |
topic |
Alternative method Monitoring Somatic cell count Udder health |
description |
The study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divided into variables related to the equipment: repeatability and reproducibility, and variables that could influencing the results as: use of preservatives, temperature, time between collection and analysis, breed and milk composition, besides analysis to relate the Ekomilk Scan® with the standard method-direct microscopy and reference-flow cytometry. As the result, for samples analysis, it shouldn’t be added preservative and these should be conducted on the same day of collection; however, temperature sample did not significantly influence results. Furthermore, Ekomilk Scan® did not show good correlation of results with the method of direct microscopy; however, it was necessary to generate equations for a positive correlation between flow cytometry and Ekomilk Scan®. Therefore, it is concluded that the equipment tested is not accurate but it can be an alternative for SCC monitoring in productive units since it uses calibration equations of results. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:54:16Z 2018-12-11T16:54:16Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0103-8478cr20170848 Ciencia Rural, v. 48, n. 6, 2018. 1678-4596 0103-8478 http://hdl.handle.net/11449/171181 10.1590/0103-8478cr20170848 S0103-84782018000600454 2-s2.0-85049506002 S0103-84782018000600454.pdf |
url |
http://dx.doi.org/10.1590/0103-8478cr20170848 http://hdl.handle.net/11449/171181 |
identifier_str_mv |
Ciencia Rural, v. 48, n. 6, 2018. 1678-4596 0103-8478 10.1590/0103-8478cr20170848 S0103-84782018000600454 2-s2.0-85049506002 S0103-84782018000600454.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ciencia Rural 0,337 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128499582500864 |